All Relations between apoptosis and blot

Reference Sentence Publish Date Extraction Date Species
Xiao Han, Jiayan Huang, Jia Lu, Feng He, Fang Wang, Shuangfeng Li, Hao Zhong, Haiyang Yu, Weiling P. Active compounds and potential targets of Shuganning injection in the treatment of hepatocellular carcinoma by network pharmacology and in vitro validation. Naunyn-Schmiedeberg's archives of pharmacology vol issue 2023 36869905 the in vitro test of the effects and mechanism of vanillin and baicalein was elucidated by mtt, western blot, immunofluorescence, and apoptosis analysis. 2023-03-04 2023-03-06 Not clear
Jawun Choi, Min-Jae Yoo, Sang-Youel Park, Jae-Won Seo. Antitumor Effects of Esculetin, a Natural Coumarin Derivative, against Canine Mammary Gland Tumor Cells by Inducing Cell Cycle Arrest and Apoptosis. Veterinary sciences vol 10 issue 2 2023 36851388 these results were supported by a western blot analysis, which revealed upregulated protein expression of cleaved caspase-3, a marker of apoptosis, and downregulated cyclin-dependent kinase 4 and cyclin d1 protein, the cell cycle regulators. 2023-02-28 2023-03-06 Not clear
Corinna Seliger, Lisa Rauer, Anne-Louise W\\xc3\\xbcster, Sylvia Moeckel, Verena Leidgens, Birgit Jachnik, Laura-Marie Ammer, Simon Heckscher, Katja Dettmer, Markus J Riemenschneider, Peter J Oefner, Martin Proescholdt, Arabel Vollmann-Zwerenz, Peter Ha. Heterogeneity of Amino Acid Profiles of Proneural and Mesenchymal Brain-Tumor Initiating Cells. International journal of molecular sciences vol 24 issue 4 2023 36834608 effects of metformin on apoptosis and autophagy were determined using western blot, annexin v/7-aad facs-analyses and a vector containing the human lc3b gene fused to green fluorescent protein. 2023-02-25 2023-03-06 human
Changjie Ren, Peiran Zhou, Mingliang Zhang, Zihao Yu, Xiaomin Zhang, Joyce Tombran-Tink, Colin J Barnstable, Xiaorong L. Molecular Mechanisms of Oxidative Stress Relief by CAPE in ARPE-19 Cells. International journal of molecular sciences vol 24 issue 4 2023 36834980 we used in situ live cell staining with cellrox and mitosox to measure ros accumulation; annexin v-fitc/pi assay to evaluate cell apoptosis; zo-1 immunostaining to observe tight junction integrity in the cells; rna-seq to analyze changes in gene expression; q-pcr to validate the rna-seq data; and western blot to examine mapk signal pathway activation. 2023-02-25 2023-03-06 Not clear
Giordana Feriotto, Riccardo Rondanin, Paolo Marchetti, Federico Tagliati, Simone Beninati, Claudio Tabolacci, Elisa Grusi, Serena Aguzzi, Carlo Mischiat. Characterization of Stable Pyrazole Derivatives of Curcumin with Improved Cytotoxicity on Osteosarcoma Cell Lines. Life (Basel, Switzerland) vol 13 issue 2 2023 36836788 induction of apoptosis was evaluated by annexin v staining and western blot analysis. 2023-02-25 2023-03-06 Not clear
Chenyuan Song, Yufei Ji, Wenzhi Wang, Ning Ta. Ginger polysaccharide promotes myeloid-derived suppressor cell apoptosis by regulating lipid metabolism. Phytotherapy research : PTR vol issue 2023 36806265 expression levels of apoptosis proteins (caspase 9 and bcl-2) and lipid metabolism enzymes (fatty acid synthase (fasn) and diacylglycerol acyltransferase 2) in msc-2 cells treated with different concentrations of ginger polysaccharide were detected by western blot assay. 2023-02-22 2023-03-06 Not clear
Xiaoying Li, Li Zhou, Ruike Wang, Yangnan Zhang, Wei L. Dihydromyricetin suppresses tumor growth via downregulation of the EGFR/Akt/survivin signaling pathway. Journal of biochemical and molecular toxicology vol issue 2023 36807944 moreover, western blot analysis indicated that dhm induced cell apoptosis via suppression of the antiapoptotic protein, survivin. 2023-02-22 2023-03-06 Not clear
Xiaoyan Hu, Lin Li, Jewel Nkwocha, Kanika Sharma, Liang Zhou, Steven Gran. Synergistic Interactions between the Hypomethylating Agent Thio-Deoxycytidine and Venetoclax in Myelodysplastic Syndrome Cells. Hematology reports vol 15 issue 1 2023 36810553 the cells were exposed to agents alone or in combination, after which apoptosis was assessed, and a western blot analysis was performed. 2023-02-22 2023-03-06 human
Hongguang Sheng, Jun Xiong, Danying Yan. Protective Effect of Sevoflurane Preconditioning on Cardiomyocytes Against Hypoxia/Reoxygenation Injury by Modulating Iron Homeostasis and Ferroptosis. Cardiovascular toxicology vol issue 2023 36800141 mtt was taken to assess the viability of each group of cells, flow cytometry to detect cell apoptosis, and qrt-pcr or western blot to detect the expression of iron metabolism-related proteins and apoptosis-related proteins in the cells. 2023-02-17 2023-03-06 rat
Mariam Sanjari-Pour, Nassim Faridi, Ping Wang, S Zahra Bathai. Protective effect of saffron carotenoids against amyloid beta-induced neurotoxicity in differentiated PC12 cells via the unfolded protein response and autophagy. Phytotherapy research : PTR vol issue 2023 36794286 rt-pcr and western blot results revealed the reduced eif2\xce\xb1 phosphorylation and increased spliced-xbp1, beclin1, lc3ii, and p62, which indicate the a\xce\xb2os-induced autophagic flux defect, autophagosome accumulation, and apoptosis. 2023-02-16 2023-03-06 Not clear
Sheng Fang, Hao Tang, Ming-Zhi Li, Jian-Jun Chu, Zong-Sheng Yin, Qi-Yu Ji. Identification of the CCL2 PI3K/Akt axis involved in autophagy and apoptosis after spinal cord injury. Metabolic brain disease vol issue 2023 36795287 we then used small interfering rna to inhibit the expression of ccl2 and pi3k to inhibit and activate the pi3k/akt/mtor signaling pathway; western blot, immunofluorescence, monodansylcadaverine, and cell flow techniques were used to detect the expression of key proteins involved in downstream autophagy and apoptosis. 2023-02-16 2023-03-06 Not clear
Yu-Hsuan Cheng, Chien-An Yao, Chih-Ching Yang, Shih-Ping Hsu, Chiang-Ting Chie. Sodium thiosulfate through preserving mitochondrial dynamics ameliorates oxidative stress induced renal apoptosis and ferroptosis in 5/6 nephrectomized rats with chronic kidney diseases. PloS one vol 18 issue 2 2023 36795670 we determined the sts effect on reactive oxygen species (ros) amount in vitro and in vivo by an ultrasensitive chemiluminescence-amplification method, ed-1 mediated inflammation, masson's trichrome stained fibrosis, mitochondrial dynamics (fission and fusion) and two types of programmed cell death, apoptosis and ferroptosis by western blot and immunohistochemistry. 2023-02-16 2023-03-06 rat
Tao Geng, Zesheng Xu, Jingxian Xing, Yonggang Yuan, Juan Li. Knockdown of lncRNA SNHG16 attenuates myocardial ischemia\\xe2\\x80\\x91reoxygenation injury via targeting miR\\xe2\\x80\\x91183/FOXO1 axis. Experimental and therapeutic medicine vol 25 issue 3 2023 36778043 cell counting kit-8, flow cytometric and western blot assays were conducted to detect cell viability, apoptosis and protein expression. 2023-02-13 2023-03-06 rat
Ozge Turna, Asuman Deveci Ozkan, Gamze Guney Eskiler, Aslihan Baykal, Ozge Ozten, Funda Yildirim, Elif Ilkay Armutak, Ali Furkan Kamanli, Hyun Soo Lim, Suleyman Kaleli, Guven Kasikci, Salih Zeki Yildi. Comparison of the anti-cancer activity of 5-aminolevulinic acid-mediated photodynamic therapy after continuous wave and pulse irradiation in different histological types of canine mammary sarcoma tumors. Lasers in medical science vol 38 issue 1 2023 36780027 however, its molecular mechanisms in the treatment of different histological types of cmst remain this context, we, for the first time, assessed 5-aminolevulinic acid (5-ala)-pdt-mediated anti-cancer activity and its molecular mechanism after continuous wave (cw) and pulse radiation (pr) on three different histological types (liposarcoma, chondrosarcoma, and osteosarcoma) of cmst cells by wst-1, annexin v, ros, acridine orange/propidium iodide staining, rt-pcr, and western blot analysis.our findings showed that 5-ala/pdt significantly suppressed the proliferation of cmst cells (p\xe2\x80\x89<\xe2\x80\x890.01) and induced apoptosis via increased ros level and overexpression of caspase-9 and caspase-3 mrna and cleaved protein levels in especially liposarcoma and chondrosarcoma cells following cw and pr irradiation at 9\xc2\xa0j/cm 2023-02-13 2023-03-06 Not clear
Peiyan He, Ziyu Li, Junzhen Zhou, Jie Yang, Xiaoyi Wei, Ping Wu, Wendan Chen, Jinxia Cheng, Li Yang, Jie Tang, Qiang Li, Qing Zhang, Jianwei Jian. Sq-2, a biotinylated annonaceous acetogenin, induces apoptosis, autophagy and S-phase arrest by activating the MAPK pathway in breast cancer cells. Acta biochimica et biophysica Sinica vol issue 2023 36762500 flow cytometry and western blot analysis reveal that sq-2 induces cell apoptosis by increasing the expressions of cleaved caspase-3 and cleaved parp as well as the ratio of bax/bcl-2. 2023-02-10 2023-03-06 Not clear
Wei Wang, Da Liu, Liyun Yang, Lixia Chen, Mengdan Miao, Yongsheng Liu, Yajuan Yin, Mei Wei, Gang Liu, Yonghui An, Mingqi Zhen. Compound Kushen injection attenuates angiotensin II\\xe2\\x80\\x91mediated heart failure by inhibiting the PI3K/Akt pathway. International journal of molecular medicine vol 51 issue 3 2023 36734284 the rate of apoptosis was detected using tunel staining and flow cytometry (fcm), and the expression levels of apoptosis\xe2\x80\x91related proteins were measured using western blot (wb) analysis. 2023-02-03 2023-03-06 mouse
Yunqi Zhao, Ying Wang, Zimeng Ling, Yaxing Xue, Deji Luan, Jian Kang, Yong Zhang, Fusheng Qua. Low-density small extracellular vesicles in bovine follicular fluid carrying let-7i target FASLG to inhibit granulosa cells apoptosis. Theriogenology vol 199 issue 2023 36716593 flow cytometry, quantitative reverse transcription pcr (rt-qpcr), western blot, and other methods were used to detect the effect of ld-sevs on follicular granulosa cell apoptosis. 2023-01-30 2023-03-06 cattle
Xin Zhu, Mei Yang, Liu Yan. Isoflurane Postconditioning Alleviates Ischemic Neuronal Injury via MiR-384-5p Regulated Autophagy. Neuroscience vol issue 2023 36707017 tunel staining and western blot results confirmed that isoflurane post-conditioning could regulate mir-384-5p and inhibit apoptosis. 2023-01-27 2023-01-31 rat
Yalong Gao, Hejun Zhang, Jiwei Wang, Fanjian Li, Xiaotian Li, Tuo Li, Cong Wang, Lei Li, Ruilong Peng, Li Liu, Weiyun Cui, Shu Zhang, Jianning Zhan. Annexin A5 ameliorates traumatic brain injury-induced neuroinflammation and neuronal ferroptosis by modulating the NF-\\xc4\\xb8B/HMGB1 and Nrf2/HO-1 pathways. International immunopharmacology vol 114 issue 2023 36700781 two days after the traumatic brain injury, we collected injured cortex tissues for western blot, perl's staining, apoptosis staining, nissl staining, immunofluorescence/immunohistochemistry, and enzyme-linked immunosorbent assay. 2023-01-26 2023-01-31 mouse
Wenqiang Xu, Xiaofeng Liu, Wenqing Qu, Xin Wang, Hao Su, Wenliang Li, Yiheng Chen. Exosomes derived from fibrinogen-like protein 1-overexpressing bone marrow-derived mesenchymal stem cells ameliorates rheumatoid arthritis. Bioengineered vol 13 issue 6 2023 36694465 cell viability, apoptosis, inflammation factors, and protein levels were detected by cck-8, flow cytometry, enzyme-linked immunosorbent assay and western blot, respectively. 2023-01-25 2023-01-31 rat