| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| J Czuwara-Ladykowska, B Makiela, E A Smith, M Trojanowska, L Rudnick. The inhibitory effects of camptothecin, a topoisomerase I inhibitor, on collagen synthesis in fibroblasts from patients with systemic sclerosis. Arthritis research. vol 3. issue 5. 2001-10-25. PMID:11549373. |
production of collagenous proteins by fibroblasts was determined in cell and matrix layers by elisa and in conditioned media by [(3)h]proline incorporation, gel electrophoresis, and autoradiography. |
2001-10-25 |
2023-08-12 |
Not clear |
| S T Pattison, J Melrose, P Ghosh, T K Taylo. Regulation of gelatinase-A (MMP-2) production by ovine intervertebral disc nucleus pulposus cells grown in alginate bead culture by Transforming Growth Factor-beta(1)and insulin like growth factor-I. Cell biology international. vol 25. issue 7. 2001-10-04. PMID:11448107. |
ovine np cells were grown in alginate bead cultures in complete medium (10% foetal calf serum) for 7 days, established in serum-free conditions for 24 h, then stimulated with tgf-beta 1 (0.1 or 10 ng/ml) or igf-i (2 or 50 ng/ml) +/-concanavalin a (20 microg/ml) for an additional 48 h. conditioned medium was examined for matrix metalloproteases using gelatin zymography, tissue inhibitor of metalloproteinase 2 (timp-2) and membrane type 1 matrix metalloproteinase (mt1-mmp) were immunolocalised in beads. |
2001-10-04 |
2023-08-12 |
Not clear |
| K H Chakrabarty, M Heaton, A J Dalley, R A Dawson, E Freedlander, P T Khaw, S Mac Nei. Keratinocyte-driven contraction of reconstructed human skin. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society. vol 9. issue 2. 2001-07-26. PMID:11350647. |
however, galardin, a matrix metalloproteinase inhibitor and keratinocyte conditioned media, both inhibited contraction. |
2001-07-26 |
2023-08-12 |
human |
| J Ohnishi, E Ohnishi, M Jin, W Hirano, D Nakane, H Matsui, A Kimura, H Sawa, K Nakayama, H Shibuya, K Nagashima, T Takahash. Cloning and characterization of a rat ortholog of MMP-23 (matrix metalloproteinase-23), a unique type of membrane-anchored matrix metalloproteinase and conditioned switching of its expression during the ovarian follicular development. Molecular endocrinology (Baltimore, Md.). vol 15. issue 5. 2001-07-19. PMID:11328856. |
cloning and characterization of a rat ortholog of mmp-23 (matrix metalloproteinase-23), a unique type of membrane-anchored matrix metalloproteinase and conditioned switching of its expression during the ovarian follicular development. |
2001-07-19 |
2023-08-12 |
mouse |
| John Martin, Lisa Eynstone, Malcolm Davies, Robert Steadma. Induction of metalloproteinases by glomerular mesangial cells stimulated by proteins of the extracellular matrix. Journal of the American Society of Nephrology : JASN. vol 12. issue 1. 2001-05-24. PMID:11134254. |
after the addition of these matrix proteins (0.1 microg/ml to 100 microg/ml) to growth-arrested hmc for 72 h, zymography of the conditioned medium established that only fibronectin and collagens i and iv dose-dependently increased latent (72 kd) mmp2 secretion and activation. |
2001-05-24 |
2023-08-12 |
human |
| A B da Rocha, D R Mans, G Lenz, A K Fernandes, C de Lima, V F Monteiro, D Gonçalves, J C Moreira, A L Brunetto, R Rodnight, G Schwartsman. Protein kinase C-mediated in vitro invasion of human glioma cells through extracellular-signal-regulated kinase and ornithine decarboxylase. Pathobiology : journal of immunopathology, molecular and cellular biology. vol 68. issue 3. 2001-04-19. PMID:11174068. |
subsequently, cells were assessed for membrane-type 1 matrix metalloproteinase (mt1-mmp) mrna contents, 72-kd latent, and 59/62-kd activated matrix metalloproteinase 2 (mmp-2) in conditioned media, as well as invasiveness. |
2001-04-19 |
2023-08-12 |
human |
| M Pohl, H Sakurai, K T Bush, S K Niga. Matrix metalloproteinases and their inhibitors regulate in vitro ureteric bud branching morphogenesis. American journal of physiology. Renal physiology. vol 279. issue 5. 2000-12-07. PMID:11053050. |
in a three-dimensional cell culture model, which employs mm cell-derived conditioned medium (bsn-cm) to induce ub cell branching morphogenesis in extracellular matrix (ecm) gels (sakurai h, barros ej, tsukamoto t, barasch j, and nigam sk. |
2000-12-07 |
2023-08-12 |
Not clear |
| U B Hofmann, J R Westphal, E T Waas, J C Becker, D J Ruiter, G N van Muije. Coexpression of integrin alpha(v)beta3 and matrix metalloproteinase-2 (MMP-2) coincides with MMP-2 activation: correlation with melanoma progression. The Journal of investigative dermatology. vol 115. issue 4. 2000-11-06. PMID:10998134. |
soluble active matrix metalloproteinase-2 was detectable only in the conditioned medium of alpha(v)beta3-negative cell lines and undetectable in the alpha(v)beta3-positive cell lines. |
2000-11-06 |
2023-08-12 |
human |
| C Trudel, V Faure-Desire, R Z Florkiewicz, A Bair. Translocation of FGF2 to the cell surface without release into conditioned media. Journal of cellular physiology. vol 185. issue 2. 2000-10-30. PMID:11025448. |
the observation that the translocation of fgf2 onto the cell surface is dissociated from its release into conditioned medium is consistent with fgf2's being rarely found in biological fluids but always cell associated and in the extracellular matrix. |
2000-10-30 |
2023-08-12 |
human |
| I Zervolea, D Kletsas, D Stathako. Autocrine regulation of proliferation and extracellular matrix homeostasis in human fibroblasts. Biochemical and biophysical research communications. vol 276. issue 2. 2000-10-27. PMID:11027548. |
furthermore, concerning extracellular matrix formation, conditioned medium has no effect on novel collagen synthesis, while it up-regulates collagenase mmp-1 only in cultures on plastic. |
2000-10-27 |
2023-08-12 |
human |
| H Izumi, C Takahashi, J Oh, M Nod. Tissue factor pathway inhibitor-2 suppresses the production of active matrix metalloproteinase-2 and is down-regulated in cells harboring activated ras oncogenes. FEBS letters. vol 481. issue 1. 2000-10-02. PMID:10984610. |
when dt cells were cultured in the presence of conditioned medium and extracellular matrix prepared from tfpi-2-transfected ht1080 cells, increased attachment and flat reversion were observed. |
2000-10-02 |
2023-08-12 |
human |
| S Nozaki, G W Sledge, H Nakshatr. Cancer cell-derived interleukin 1alpha contributes to autocrine and paracrine induction of pro-metastatic genes in breast cancer. Biochemical and biophysical research communications. vol 275. issue 1. 2000-09-21. PMID:10944441. |
in addition, this antibody also prevented induction of il-6, il-8, and matrix metalloproteinase 3 (mmp3) but not vascular endothelial growth factor (vegf) in fibroblasts by conditioned medium (cm) from il-1alpha-expressing breast cancer cells. |
2000-09-21 |
2023-08-12 |
Not clear |
| N Buisson-Legendre, H Emonard, P Bernard, W Hornebec. Relationship between cell-associated matrix metalloproteinase 9 and psoriatic keratinocyte growth. The Journal of investigative dermatology. vol 115. issue 2. 2000-09-14. PMID:10951238. |
whether cells were treated with sphingomyelinase or not, however, conditioned media from psoriatic keratinocytes contained higher levels of tissue inhibitor of metalloproteinase-1 compared with matrix metalloproteinase 9 and secreted only the proenzyme form. |
2000-09-14 |
2023-08-12 |
Not clear |
| J I Fenton, K A Chlebek-Brown, T L Peters, J P Caron, M W Ort. Glucosamine HCl reduces equine articular cartilage degradation in explant culture. Osteoarthritis and cartilage. vol 8. issue 4. 2000-09-12. PMID:10903879. |
nitric oxide, proteoglycan and matrix metalloproteinases (mmp) released into conditioned media and tissue proteoglycan synthesis were measured as indicators of cartilage metabolism. |
2000-09-12 |
2023-08-12 |
human |
| K Meyer-Siegle. Macrophage migration inhibitory factor increases MMP-2 activity in DU-145 prostate cells. Cytokine. vol 12. issue 7. 2000-09-08. PMID:10880236. |
elisa was used to screen conditioned medium for the production of mif, matrix metalloproteinase-2 (mmp-2) and tissue inhibitor of metalloproteinase-2 (timp-2). |
2000-09-08 |
2023-08-12 |
Not clear |
| M Maeda-Yamamoto, H Kawahara, N Tahara, K Tsuji, Y Hara, M Isemur. Effects of tea polyphenols on the invasion and matrix metalloproteinases activities of human fibrosarcoma HT1080 cells. Journal of agricultural and food chemistry. vol 47. issue 6. 2000-08-16. PMID:10794635. |
ester-type catechins (ecg and egcg) and theaflavin strongly suppressed the gelatin degradation mediated by matrix metalloproteinase (mmp) 2 and mmp-9, which were secreted into the conditioned medium of ht1080 cells. |
2000-08-16 |
2023-08-12 |
human |
| E Toschi, R Rota, A Antonini, G Melillo, M C Capogross. Wild-type p53 gene transfer inhibits invasion and reduces matrix metalloproteinase-2 levels in p53-mutated human melanoma cells. The Journal of investigative dermatology. vol 114. issue 6. 2000-07-17. PMID:10844565. |
this reduced invasiveness was associated with decreased matrix metalloproteinase-2 levels in conditioned media whereas no changes were detected in matrix metalloproteinase-9 secreted levels. |
2000-07-17 |
2023-08-12 |
human |
| S Sanghi, R Kumar, S Walton, G W Lauri. Quantitation of rat lacrimal secretion: a novel sandwich ELISA with high sensitivity. Experimental eye research. vol 70. issue 5. 2000-07-10. PMID:10870523. |
functional screens of recombinant protein products arising from gene array technologies, or protein fractions derived from lacrimal conditioned media or extracellular matrix, will require a highly sensitive assay capable of monitoring tear protein secretion by small replicate cultures. |
2000-07-10 |
2023-08-12 |
human |
| O H Sanchez-Sweatman, F W Orr, G Sing. Human metastatic prostate PC3 cell lines degrade bone using matrix metalloproteinases. Invasion & metastasis. vol 18. issue 5-6. 2000-06-27. PMID:10729774. |
a role for matrix metalloproteinases in degradation was shown by: (1) stimulation by the phorbol ester tpa of pc3-induced matrix degradation and release of matrix metalloproteinase activity; (2) abrogation of matrix degradation by 1,10-phenanthroline, a metalloproteinase inhibitor, and (3) degradation of purified type i collagen by pc3 cells and their conditioned medium. |
2000-06-27 |
2023-08-12 |
human |
| M Mizuno, T Imai, R Fujisawa, H Tani, Y Kubok. Bone sialoprotein (BSP) is a crucial factor for the expression of osteoblastic phenotypes of bone marrow cells cultured on type I collagen matrix. Calcified tissue international. vol 66. issue 5. 2000-06-19. PMID:10773110. |
cells showed osteoblastic phenotypes (high alkaline phosphatase activity, osteocalcin synthesis, and responsiveness against parathyroid hormone) on collagen matrix gels at week 3 after the inoculation, and simultaneously, bsp was detected in the conditioned medium by western blotting using an anti-bsp monoclonal antibody. |
2000-06-19 |
2023-08-12 |
rat |