| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| G D Pins, M E Collins-Pavao, L Van De Water, M L Yarmush, J R Morga. Plasmin triggers rapid contraction and degradation of fibroblast-populated collagen lattices. The Journal of investigative dermatology. vol 114. issue 4. 2000-06-01. PMID:10733668. |
western blot analyses of conditioned medium demonstrated that fibroblasts in collagen lattices secreted the latent matrix metalloproteinase, mmp-1, which was subsequently cleaved by plasmin. |
2000-06-01 |
2023-08-12 |
human |
| E J Bates, N M Penno, P I Hyn. Wool follicle matrix cells: culture conditions and keratin expression in vitro. The British journal of dermatology. vol 140. issue 2. 2000-03-17. PMID:10733269. |
extracellular matrix from dermal papillae, conditioned media, separation of dermal papilla from bulb matrices by tissue culture inserts and feeder layers were unable to support matrix cell explantation. |
2000-03-17 |
2023-08-12 |
Not clear |
| J L Hao, T Nagano, M Nakamura, N Kumagai, H Mishima, T Nishid. Effect of galardin on collagen degradation by Pseudomonas aeruginosa. Experimental eye research. vol 69. issue 6. 2000-02-24. PMID:10620388. |
the conditioned media were also subjected to gelatin zymography and western blotting to analyse the activation, by p. aeruginosa factor(s), of matrix metalloproteinases (mmps) released by keratocytes. |
2000-02-24 |
2023-08-12 |
Not clear |
| M Yan, T G Nick, A R Sinnin. Retinoic acid inhibition of cardiac mesenchyme formation in vitro correlates with changes in the secretion of particulate matrix from the myocardium. The Anatomical record. vol 258. issue 2. 2000-02-18. PMID:10645966. |
initial experiments tested the effect of retinoic acid on mesenchymal cell formation first in atrioventricular canal and outflow tract explant cultures and then in av endothelial monolayer cultures using myocyte conditioned media or the particulate matrix fraction from retinoic acid treated myocyte cultures. |
2000-02-18 |
2023-08-12 |
mouse |
| J C Irwin, L F Suen, B H Cheng, R Martin, P Cannon, C L Deal, L C Giudic. Human placental trophoblasts secrete a disintegrin metalloproteinase very similar to the insulin-like growth factor binding protein-3 protease in human pregnancy serum. Endocrinology. vol 141. issue 2. 2000-02-10. PMID:10650948. |
igfbp-3 proteolysis by both pregnancy serum and trophoblast conditioned medium was also insensitive to tissue inhibitor of metalloproteinase-1, precluding the involvement of the matrix metalloproteinases. |
2000-02-10 |
2023-08-12 |
human |
| I Bernard-Pierrot, M Héroult, G Lemaître, D Barritault, J Courty, P E Milhie. Glycosaminoglycans promote HARP/PTN dimerization. Biochemical and biophysical research communications. vol 266. issue 2. 2000-01-18. PMID:10600521. |
the relevance of this process was supported by experiments demonstrating that harp is secreted as a dimer in conditioned medium of nih-3t3 cells that overexpressed this growth factor and is also associated to the cell surface or to the extracellular matrix. |
2000-01-18 |
2023-08-12 |
human |
| F A Mercuri, K J Doege, E C Arner, M A Pratta, K Last, A J Fosan. Recombinant human aggrecan G1-G2 exhibits native binding properties and substrate specificity for matrix metalloproteinases and aggrecanase. The Journal of biological chemistry. vol 274. issue 45. 1999-12-13. PMID:10542281. |
the size of rg1-g2 by sds-polyacrylamide gel electorphoresis was unchanged following digestion with keratanase and keratanase ii and reduced by only 2-5 kda following digestion with either o-glycosidase or n-glycosidase f. recombinant g1-g2 was digested with purified matrix metalloproteinases (mmp), isolated aggrecanase, purified atrolysin c, or proteinases present in conditioned medium from cartilage explant cultures, and the products analyzed on sds gels by silver stain and immunoblotting. |
1999-12-13 |
2023-08-12 |
human |
| A Pavlova, R O Stuart, M Pohl, S K Niga. Evolution of gene expression patterns in a model of branching morphogenesis. The American journal of physiology. vol 277. issue 4. 1999-11-17. PMID:10516290. |
an established cell culture model for this process employs cells of ureteric bud origin (ub) cultured in extracellular matrix and stimulated with conditioned media (bsn-cm) from a metanephric mesenchymal cell line (h. sakurai, e. j. barros, t. tsukamoto, j. barasch, and s. k. nigam. |
1999-11-17 |
2023-08-12 |
Not clear |
| A N Neely, C E Clendening, J Gardner, D G Greenhalgh, G D Warde. Gelatinase activity in keloids and hypertrophic scars. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society. vol 7. issue 3. 1999-09-10. PMID:10417752. |
identity of matrix metalloproteinases was determined using a conditioned media reference standard, molecular weight ladders, and western blotting. |
1999-09-10 |
2023-08-12 |
Not clear |
| H Bismar, T Klöppinger, E M Schuster, S Balbach, I Diel, R Ziegler, J Pfeilschifte. Transforming growth factor beta (TGF-beta) levels in the conditioned media of human bone cells: relationship to donor age, bone volume, and concentration of TGF-beta in human bone matrix in vivo. Bone. vol 24. issue 6. 1999-08-20. PMID:10375198. |
transforming growth factor beta (tgf-beta) levels in the conditioned media of human bone cells: relationship to donor age, bone volume, and concentration of tgf-beta in human bone matrix in vivo. |
1999-08-20 |
2023-08-12 |
human |
| X H Pei, Y Nakanishi, K Takayama, F Bai, N Har. Granulocyte, granulocyte-macrophage, and macrophage colony-stimulating factors can stimulate the invasive capacity of human lung cancer cells. British journal of cancer. vol 79. issue 1. 1999-07-29. PMID:10408691. |
gelatin zymographs of conditioned media derived from human lung adenocarcinoma cell lines revealed two major bands of gelatinase activity at 68 and 92 kda, which were characterized as matrix metalloproteinase (mmp)-2 and mmp-9 respectively. |
1999-07-29 |
2023-08-12 |
human |
| I Fajardo, A R Quesada, I Núñez de Castro, F Sánchez-Jiménez, M A Medin. A comparative study of the effects of genistein and 2-methoxyestradiol on the proteolytic balance and tumour cell proliferation. British journal of cancer. vol 80. issue 1-2. 1999-07-07. PMID:10389972. |
since the degradation of the extracellular matrix is one of the essential steps in angiogenesis, the potential modulatory effects of both compounds on the proteolytic balance in media conditioned by different human tumour cells have been also investigated. |
1999-07-07 |
2023-08-12 |
human |
| C Festuccia, M Bologna, G L Gravina, F Guerra, A Angelucci, I Villanova, D Millimaggi, A Tet. Osteoblast conditioned media contain TGF-beta1 and modulate the migration of prostate tumor cells and their interactions with extracellular matrix components. International journal of cancer. vol 81. issue 3. 1999-04-29. PMID:10209954. |
osteoblast conditioned media contain tgf-beta1 and modulate the migration of prostate tumor cells and their interactions with extracellular matrix components. |
1999-04-29 |
2023-08-12 |
human |
| S Luikart, D Wahl, T Hinkel, M Masri, T Oegem. A fragment of alpha-actinin promotes monocyte/macrophage maturation in vitro. Experimental hematology. vol 27. issue 2. 1999-03-11. PMID:10029173. |
conditioned media (cm) from cultures of hl-60 myeloid leukemia cells grown on extracellular bone marrow matrix contains a factor that induces macrophage-like maturation of hl-60 cells. |
1999-03-11 |
2023-08-12 |
human |
| Y Wu, A J Palad, W J Wasilenko, P F Blackmore, W A Pincus, G L Schechter, J R Spoonster, E C Kohn, K D Somer. Inhibition of head and neck squamous cell carcinoma growth and invasion by the calcium influx inhibitor carboxyamido-triazole. Clinical cancer research : an official journal of the American Association for Cancer Research. vol 3. issue 11. 1999-02-25. PMID:9815580. |
conditioned medium from hnscc cell lines was analyzed by zymography for production of mr 72,000 type iv collagenase [matrix metalloproteinase (mmp)-2)] and mr 92,000 type iv collagenase (mmp-9). |
1999-02-25 |
2023-08-12 |
Not clear |
| F Kikkawa, A Nawa, K Shibata, N H Obata, K Tamakoshi, S Suzuki, S Mizutan. The different stimulatory effect of normal tissues on the secretion of matrix metalloproteinases and their inhibitors by human ovarian cancer cells. Anticancer research. vol 18. issue 6A. 1999-02-11. PMID:9891486. |
to investigate the influence of normal tissues on secretion of matrix metalloproteinases (mmps) and their inhibitors (timps) by ovarian cancer cells, conditioned medium of several normal human tissues (peritoneum, myometrium, and ovary) was added to ovarian cancer cells (nom1). |
1999-02-11 |
2023-08-12 |
human |
| M Mäkelä, T Salo, H Larjav. MMP-9 from TNF alpha-stimulated keratinocytes binds to cell membranes and type I collagen: a cause for extended matrix degradation in inflammation? Biochemical and biophysical research communications. vol 253. issue 2. 1999-01-20. PMID:9878537. |
type i and iv collagens and gelatin but no other purified extracellular matrix nor basement membrane proteins (types i and iv collagen, laminin-1 and -5, fibronectin) were able to bind mmp-9 from the conditioned medium. |
1999-01-20 |
2023-08-12 |
Not clear |
| C Moss, P W Beesley, M C Thorndyke, T Bollne. Preliminary observations on ascidian and echinoderm neurons and neural explants in vitro. Tissue & cell. vol 30. issue 5. 1998-12-22. PMID:9839474. |
attempts were made to stimulate neuron survival and outgrowth of echinoderm cultures with conditioned media containing growth factors or tissue extracts and with various substrates including extracellular matrix extracts from native tissue. |
1998-12-22 |
2023-08-12 |
Not clear |
| B S Weeks, M Nomizu, R S Ramchandran, Y Yamada, H K Kleinma. Laminin-1 and the RKRLQVQLSIRT laminin-1 alpha1 globular domain peptide stimulate matrix metalloproteinase secretion by PC12 cells. Experimental cell research. vol 243. issue 2. 1998-10-27. PMID:9743597. |
zymographic analysis of conditioned media from laminin-1-treated pc12 and ng108-15 cells revealed a 72-kda matrix metalloproteinase which was not secreted by untreated cells. |
1998-10-27 |
2023-08-12 |
Not clear |
| H R Lijnen, J Silence, G Lemmens, L Frederix, D Colle. Regulation of gelatinase activity in mice with targeted inactivation of components of the plasminogen/plasmin system. Thrombosis and haemostasis. vol 79. issue 6. 1998-10-16. PMID:9657444. |
to investigate a potential physiological role of the plasminogen/plasmin system in activation of the matrix metalloproteinase (mmp) system, the distribution of latent and active mmp-2 (gelatinase a) or mmp-9 (gelatinase b) was monitored in aorta extracts and in serum-free conditioned cell culture medium obtained from wild-type (wt) mice and from mice with deficiency of tissue-type plasminogen activator (t-pa(-/-)), urokinase-type plasminogen activator (u-pa(-/-)), plasminogen activator inhibitor-1 (pai-1(-/-)) or plasminogen (plg(-/-)). |
1998-10-16 |
2023-08-12 |
mouse |