| Reference |
Sentence |
Publish Date |
Extraction Date |
Species |
| Xiao Han, Jiayan Huang, Jia Lu, Feng He, Fang Wang, Shuangfeng Li, Hao Zhong, Haiyang Yu, Weiling P. Active compounds and potential targets of Shuganning injection in the treatment of hepatocellular carcinoma by network pharmacology and in vitro validation. Naunyn-Schmiedeberg's archives of pharmacology vol issue 2023 36869905 |
the in vitro test of the effects and mechanism of vanillin and baicalein was elucidated by mtt, western blot, immunofluorescence, and apoptosis analysis. |
2023-03-04 |
2023-03-06 |
Not clear |
| Hongguang Sheng, Jun Xiong, Danying Yan. Protective Effect of Sevoflurane Preconditioning on Cardiomyocytes Against Hypoxia/Reoxygenation Injury by Modulating Iron Homeostasis and Ferroptosis. Cardiovascular toxicology vol issue 2023 36800141 |
mtt was taken to assess the viability of each group of cells, flow cytometry to detect cell apoptosis, and qrt-pcr or western blot to detect the expression of iron metabolism-related proteins and apoptosis-related proteins in the cells. |
2023-02-17 |
2023-03-06 |
rat |
| Rajeev Shrestha, Hyun Ji Lee, Junmo Lim, Pallavi Gurung, Til Bahadur Thapa Magar, Young-Tak Kim, Kija Lee, Seulgi Bae, Yong-Wan Ki. Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors. Life (Basel, Switzerland) vol 12 issue 12 2022 36556469 |
cell proliferation and apoptosis were detected by the 4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) assay and western blot assay, respectively. |
2022-12-23 |
2023-01-30 |
mouse |
| Nan Zheng, Shan Liu, Huiting Zeng, Huajun Zhao, Lixu Ji. Molecular Mechanism of Curcumin Derivative on YAP Pathway against Ovarian Cancer. Journal of clinical medicine vol 11 issue 23 2022 36498794 |
mtt assay was used to detect the effect of wz10 on the proliferation of ovarian cancer ovcar3 cells; annexin v/pi double staining flow cytometry was used to detect the effect of wz10 on cell apoptosis; transwell method was used to detect the effect of wz10 on cell invasiveness; western blot was used to investigate the effect of wz10 mechanisms affecting ovcar3 activity in ovarian cancer in vitro. |
2022-12-11 |
2023-01-30 |
Not clear |
| Rui Zhang, Ziqi Meng, Xuwei Wu, Meihua Zhang, Zhengri Piao, Tiefeng Ji. PD-L1/p-STAT3 promotes the progression of NSCLC cells by regulating TAM polarization. Journal of cellular and molecular medicine vol issue 2022 36372977 |
after constructing the co-culture system and conditioned medium system, the proliferation, apoptosis, metastasis, angiogenesis, emt process and stemness of nsclc were detected by mtt, flow cytometry, transwell, endothelial cell tube formation and western blot assays. |
2022-11-14 |
2023-01-30 |
Not clear |
| Seda \\xc5\\x9eirin, Hatice G\\xc3\\xbcl Dursun, Canan Ero\\xc4\\x9flu G\\xc3\\xbcne\\xc5\\x9. Composition analysis of Salsola grandis and its effects on colon cancer cells. Anti-cancer agents in medicinal chemistry vol issue 2022 36305129 |
we applied 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide, the thiazolyl blue (mtt) method to evaluate its effects on cell viability, elisa assay, real time pcr and western blot method to reveal its effects on apoptosis. |
2022-10-28 |
2023-01-30 |
Not clear |
| Aleksandra Jovanovi\\xc4\\x87 Galovi\\xc4\\x87, Nata\\xc5\\xa1a Jovanovi\\xc4\\x87 Lje\\xc5\\xa1kovi\\xc4\\x87, Senka Vidovi\\xc4\\x87, Jelena Vladi\\xc4\\x87, Nikola Joji\\xc4\\x87, Milan Ili\\xc4\\x87, Tatjana Srdi\\xc4\\x87 Raji\\xc4\\x87, Vesna Koji\\xc4\\x87, Dimitar Jakimo. The Effects of Resveratrol-Rich Extracts of Pharmaceutics vol 14 issue 10 2022 36297452 |
the cytotoxic effect is assessed by the mtt test and eb/ao staining, levels of apoptosis are determined by annexin v assay, autophagia by ulk-1 expression using western blot and nf-kb activation by p65 elisa. |
2022-10-27 |
2023-01-30 |
Not clear |
| Xianyan Wang, Hongmei Li, Ning Zhang, Tian Wu, Shaoqing Wan. FGFR2-CCDC6 fusion gene promotes the proliferation of Hucct-1 cells. Cellular and molecular biology (Noisy-le-Grand, France) vol 68 issue 6 2022 36227665 |
the effect of the fusion gene on cell proliferation was examined by mtt and the expression of fgfr2/akt/signaling pathway proteins was detected by western blot. |
2022-10-13 |
2023-01-30 |
Not clear |
| Sicheng Chen, Mengjun Bie, Xiaowen Wang, Mengtian Fan, Bin Chen, Qiong Shi, Yingjiu Jian. PGRN exacerbates the progression of non-small cell lung cancer via PI3K/AKT/Bcl-2 antiapoptotic signaling. Genes & diseases vol 9 issue 6 2022 36157487 |
the proliferation, apoptosis, migration, and invasion of nsclc cells were assessed by the mtt assay, western blot, degree of wound healing, and transwell assays. |
2022-09-26 |
2023-01-30 |
mouse |
| Tao Zou, Shan Gao, Zhaolan Yu, Fuyong Zhang, Lan Yao, Mengyao Xu, Junxin Li, Zhigui Wu, Yilan Huang, Shurong Wan. Salvianolic acid B inhibits RAW264.7 cell polarization towards the M1 phenotype by inhibiting NF-\\xce\\xbaB and Akt/mTOR pathway activation. Scientific reports vol 12 issue 1 2022 35974091 |
the effects of different treatments on cell viability, gene expression and secretion of related proteins, phenotypic markers and cytokines were detected by mtt and western blot assays, rt\xe2\x80\x92qpcr and elisas. |
2022-08-16 |
2023-01-30 |
Not clear |
| Jiangqi Xu, Xiaohong Huang, Shixiang Liu, Dongdong Chen, Yufang Xie, Zhenwu Zha. The protective effects of lncRNA ZFAS1/miR-421-3p/MEF2C axis on cerebral ischemia-reperfusion injury. Cell cycle (Georgetown, Tex.) vol issue 2022 35880950 |
overexpressed or silenced plasmids of zfas1 were transfected into the cells to analyze the effects of oxygen-glucose deprivation/reperfusion (ogd/r) treatment on the viability, apoptosis and related gene expressions of neuro-2a cell by performing mtt assay, flow cytometry, qrt-pcr, and western blot. |
2022-07-26 |
2023-01-30 |
mouse |
| Yei-Jin Kang, Won-Geun Yang, Weon-Sik Chae, Dae-Won Kim, Seong-Gon Kim, Horatiu Rotar. Administration of 4\\xe2\\x80\\x91hexylresorcinol increases p53\\xe2\\x80\\x91mediated transcriptional activity in oral cancer cells with the p53 mutation. Oncology reports vol 48 issue 3 2022 35856441 |
to determine the effect of 4hr on p53 mutant carcinoma cells, western blot analysis, p53 transcriptional activity analysis, mtt assay and apoptosis immunocytochemistry were performed. |
2022-07-20 |
2023-01-30 |
human |
| Zhiguo Wu, Jian Tan, Lichang Lin, Wenting Zhang, Wanqiu Yua. microRNA-140-3p protects hippocampal neuron against pyroptosis to attenuate sevoflurane inhalation-induced post-operative cognitive dysfunction in rats via activation of HTR2A/ERK/Nrf2 axis by targeting DNMT1. Cell death discovery vol 8 issue 1 2022 35710537 |
in neurons, cell viability, apoptosis, and pyroptosis-related factors were tested by mtt, flow cytometry, and western blot analysis respectively. |
2022-06-16 |
2023-01-27 |
rat |
| Valeria Consoli, Valeria Sorrenti, Valeria Pittal\\xc3\\xa0, Khaled Greish, Agata Grazia D'Amico, Giuseppe Romeo, Sebastiano Intagliata, Loredana Salerno, Luca Vanell. Heme Oxygenase Modulation Drives Ferroptosis in TNBC Cells. International journal of molecular sciences vol 23 issue 10 2022 35628518 |
ho's effect on bc was evaluated by mtt tests, gene silencing, western blot analysis, and measurement of reactive oxygen species (ros), glutathione (gsh) and lipid hydroperoxide (looh) levels. |
2022-05-28 |
2023-01-27 |
Not clear |
| Song Qi, Li Xu, Yongyuan Han, Hongkun Chen, Anyuan Chen. miR-29a-3p mitigates the development of osteosarcoma through modulating IGF1 mediated PI3k/Akt/FOXO3 pathway by activating autophagy. Cell cycle (Georgetown, Tex.) vol issue 2022 35575588 |
then, the 3- (4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (mtt) assay, colony formation experiment, western blot and transwell assay were conducted to validate os cell proliferation, colony formation ability, apoptosis, migration and invasion. |
2022-05-16 |
2023-01-27 |
Not clear |
| Zhitao Yao, Wei An, Adili Moming, Maimaitituxun Tuerd. Long non-coding RNA TUG1 knockdown repressed the viability, migration and differentiation of osteoblasts by sponging miR-214. Experimental and therapeutic medicine vol 23 issue 3 2022 35126706 |
after transfection with small interfering (si)-tug1, microrna (mir)-214 mimic, mir-214 inhibitor, bone morphogenetic protein 2 (bmp2) mimic or a combination, the biological behavior of osteoblasts was evaluated using mtt, transwell assays, rt-qpcr, flow cytometry and western blot analysis. |
2022-02-07 |
2023-01-27 |
human |
| Xiaoxiao Tao, Yaping Jiang, Xian Zheng, Xiaoxiao Ji, Feifei Pen. Dihydromyricetin ameliorates oxygen\\xe2\\x80\\x91glucose deprivation and re\\xe2\\x80\\x91oxygenation\\xe2\\x80\\x91induced injury in HT22 cells by activating the Wnt/\\xce\\xb2\\xe2\\x80\\x91catenin signaling pathway. Molecular medicine reports vol 25 issue 3 2022 35088876 |
the effects of dmy on the viability, oxidative stress and apoptosis of ht22 cells following oxygen\xe2\x80\x91glucose deprivation and re\xe2\x80\x91oxygenation (ogd/r) were examined using mtt, lactate dehydrogenase (ldh), superoxide (sod), malondialdehyde (mda), western blot and tunel assays. |
2022-01-28 |
2023-01-27 |
Not clear |
| Dan Liu, Ye Cheng, Zhipeng Tang, Junliang Chen, Ying Xia, Chengbin Xu, Xiangyu Ca. Potential mechanisms of methylglyoxal-induced human embryonic kidney cells damage: Regulation of oxidative stress, DNA damage, and apoptosis. Chemistry & biodiversity vol issue 2021 34962083 |
cell viability, apoptosis and the signaling pathways were measured with mtt, fluorescent staining and western blot experiments, the results showed that mgo could induce oxidative stress and cell inflammation, the level of reactive oxygen species (ros) increased, and p38mapk, jnk and nf-\xce\xbab signaling pathways were activated. |
2021-12-28 |
2023-01-26 |
human |
| Ziheng Li, Ying Zhang, Yuan Zhou, Fuqian Wang, Chao Yin, Li Ding, Shunbo Zhan. Tanshinone IIA suppresses the progression of lung adenocarcinoma through regulating CCNA2-CDK2 complex and AURKA/PLK1 pathway. Scientific reports vol 11 issue 1 2021 34880385 |
our experiments in vitro focused on the anti-luad effects and mechanisms of tsa on luad cells (a549 and nci-h1975 cells) via mtt, plate cloning, annexin v-fitc and pi dual staining, flow cytometry, and western blot assays. |
2021-12-12 |
2023-01-26 |
Not clear |
| Li-Te Chin, Ke-Wei Liu, Yi-Han Chen, Shu-Ching Hsu, Lin Huan. Cell-based assays and molecular simulation reveal that the anti-cancer harmine is a specific matrix metalloproteinase-3 (MMP-3) inhibitor. Computational biology and chemistry vol 94 issue 2021 34384998 |
the human glioma cancer cell line, u-87 mg cells, was subjected to different concentrations (1-10 \xce\xbcm) of harmine for 24 h. methylthiazol tetrazolium (mtt) test, half maximal inhibitory concentration (ic50), western blot analysis, enzyme-linked immunosorbent assay and molecular docking through biovia discoverystudio\xe2\x84\xa2 were performed. |
2021-11-03 |
2023-01-26 |
Not clear |