All Relations between blot and mtt

Publication Sentence Publish Date Extraction Date Species
Qianrui Huang, Mengling Wu, Yamin Pu, Junyou Zhou, Yiqian Zhang, Ru Li, Yong Xia, Yiwen Zhang, Yimei M. Inhibition of TNBC Cell Growth by Paroxetine: Induction of Apoptosis and Blockage of Autophagy Flux. Cancers. vol 16. issue 5. 2024-03-13. PMID:38473249. cell viability was measured using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h-tetrazolium bromide (mtt) assay, apoptosis was assessed through flow cytometry, and the effects on signaling pathways were analyzed using rna sequencing and western blot techniques. 2024-03-13 2024-03-15 Not clear
Haihua Hong, Dongxiao Ding, Yonghua Zhang, Yongbin Chen, Shiyuan Chen, Maofen Jiang, Hairong Zhang, Qinqin Wang, Yue Hu, Jianghong He, Jiawei Yua. Circ_BLNK is a Unique Molecular Marker in Non-small Cell Lung Cancer. Biochemical genetics. 2024-02-27. PMID:38411943. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-h-tetrazolium bromide (mtt) assay, 5-ethynyl-2'-deoxyuridine (edu) assay and colony formation assay detected cell proliferation; the protein expression levels were tested by western blot assay; cell apoptosis was measured by flow cytometry, and transwell assay detected cell migration and invasion. 2024-02-27 2024-03-02 Not clear
Lihong Zhang, Qingzi Guo, Ran An, Shuhan Shen, Lin Yi. In vitro ischemic preconditioning mediates the Ca Brain research. 2023-12-23. PMID:38141801. cell viability was detected by mtt, apoptosis by hoechst 33,258 staining, the levels of tnf-α and il-1β mrna by rt-qpcr and elisa, and the levels of can, nfat, cox-2 by rt-qpcr and western blot. 2023-12-23 2023-12-25 Not clear
Ling-Ray Tai, Yi-Fen Chiang, Ko-Chieh Huang, Hsin-Yuan Chen, Mohamed Ali, Shih-Min Hsi. Hinokitiol as a modulator of TLR4 signaling and apoptotic pathways in atopic dermatitis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 170. 2023-12-21. PMID:38128179. additionally, the cell culture model enabled the evaluation of apoptosis and ros levels using mtt assay, crystal violet staining, western blot, and dcfda assays. 2023-12-21 2023-12-24 Not clear
Ramadan F Abdelaziz, Ahmed M Hussein, Mohamed H Kotob, Christina Weiss, Krzysztof Chelminski, Tamara Stojanovic, Christian R Studenik, Mohammed Auf. Enhancement of Radiation Sensitivity by Cathepsin L Suppression in Colon Carcinoma Cells. International journal of molecular sciences. vol 24. issue 23. 2023-12-09. PMID:38069428. in addition, the study conducted protein expression analysis by western blot and immunofluorescence assay, cytotoxicity mtt, and apoptosis assays. 2023-12-09 2023-12-17 Not clear
Zhifang Tang, Jingyuan Li, Chuan L. Post-Transcriptional Regulator RBM47 Stabilizes FBXO2 mRNA to Advance Osteoarthritis Development: WGCNA Analysis and Experimental Validation. Biochemical genetics. 2023-12-09. PMID:38070024. functional analysis results from the mtt assay, flow cytometry, evaluation of ldh activities and inflammatory mediators, and western blot analysis of extracellular matrix (ecm) proteins, showed that rbm47 knockdown significantly alleviated inflammation, apoptosis, and ecm degradation in interleukin 1β (il-1β)-treated chondrocytes. 2023-12-09 2023-12-17 Not clear
Xixi Li, Zhipeng Huang, Hua Chao, Xunxun Li. The mechanism of Semen Persicae-Flos Carthami in treating hypertrophic scar: A study based on network pharmacological analysis and in vitro experiments. Chemical biology & drug design. 2023-12-06. PMID:38056934. besides, a modified explant culture method was applied to separate primary hypertrophic scar fibroblasts (hsfs); mtt assay to detect cell viability of hsfs after treatment by sp-fc for 24 h; annexin v-fitc/pi double staining combined with flow cytometry to test apoptosis; western blot to check the protein expression level of p53; and real-time fluorescence quantitative pcr to determine mrna level of p53. 2023-12-06 2023-12-10 Not clear
Jian Hao, Zhenhan Li, Li Xie, Bingbing Yu, Boyuan Ma, Yubiao Yang, Xuchen Ma, Bitao Wang, Xianhu Zho. Syringaresinol promotes the recovery of spinal cord injury by inhibiting neuron apoptosis via activating the ubiquitination factor E4B/AKT Serine/Threonine kinase signal pathway. Brain research. 2023-11-22. PMID:37992795. second, mtt was employed to evaluate cell proliferation rate, western blot was performed to detect protein expression, rt-qpcr was conducted to detect mrna expression levels, flow cytometry and 5-ethynyl-2'-deoxyuridine (edu) staining were used to determine cell apoptosis, and immunofluorescence and immunohistochemistry were used to estimate the expression of rna binding fox-1 homolog 3 and clipped caspase 3. 2023-11-22 2023-11-29 rat
Huifang Zhang, Yudan Zhang, Kaixuan Xu, Lawen Wang, Xin Zhou, Mingge Yang, Jiangli Xie, Hui L. Inhibition of FLT1 Attenuates Neurodevelopmental Abnormalities and Cognitive Impairment in Offspring Caused by Maternal Prenatal Stress. Applied biochemistry and biotechnology. 2023-11-18. PMID:37979086. the apoptosis level of hippocampal neurons and the expression of nmdars were detected by mtt assay, tunel assay, and western blot. 2023-11-18 2023-11-20 rat
Ying-Jie Zhu, Hu-Lin Chen, Xin-Jie Cai, Bang-le Zhan, Xiao-Ming Li. Naringin acts as a TRPV1 antagonist to attenuate UVB-induced senescence and damage in HaCaT cells. Chemical biology & drug design. 2023-11-09. PMID:37945519. analysis was performed using the mtt assay to assess cell viability, flow cytometry to measure the apoptosis level, sa-β-gal staining to observe cellular senescence, and western blot to assess protein levels of trpv1, p16, p53, p21, matrix metalloproteinase (mmp)-1, and mmp-9. 2023-11-09 2023-11-20 human
Linlin Yue, Xin Liu, Chaoyu Wu, Jiying Lai, Jie Wang, Huifeng Zhong, Feng Che. Toll-like receptor 4 promotes the inflammatory response in septic acute kidney injury by promoting p38 mitogen-activated protein kinase phosphorylation. Journal of bioenergetics and biomembranes. 2023-08-21. PMID:37605037. cell viability, inflammatory factor (tnf-α, il-6, il-4, il-1β, il-18) levels, pyroptotic cell number changes, lactate dehydrogenase (ldh) activity, myeloperoxidase (mop) concentration, and levels of pyroptosis-associated protein and myd88, trif and p38 mapk phosphorylation were determined by mtt, elisa, fam-flica caspase-1 detection kit, other corresponding kits, and western blot. 2023-08-21 2023-09-07 mouse
Mohammad Rasoul Samandari-Bahraseman, Babak Khorsand, Sara Zareei, Massoud Amanlou, Hanieh Rostamabad. Various concentrations of hesperetin induce different types of programmed cell death in human breast cancerous and normal cell lines in a ROS-dependent manner. Chemico-biological interactions. 2023-07-24. PMID:37487865. by using mtt assay, real-time pcr, western blot, and flow cytometry, we determined the effects of hst on cell viability, ros levels, and markers of cell death. 2023-07-24 2023-08-14 human
Yan Li, Ying Zhang, Lei Gua. MiR-708 regulates the biological behavior of childhood leukemia cells by binding to the 3'utr end of the target gene and reducing the level of the target gene. Cellular and molecular biology (Noisy-le-Grand, France). vol 69. issue 2. 2023-05-25. PMID:37224029. mtt assay was used to detect the cell proliferation inhibition rate, flow cytometry was used to detect the apoptosis rate and cell cycle change, the scratch test was used to detect the cell migration capacity, and western blot assay was used to detect the expression of cntfr, apoptosis and jak/stat pathway related proteins. 2023-05-25 2023-08-14 human
Jing-Yu Qu, Hua-Tao Xie, Yu-Ting Xiao, Ying-Ying Zhang, Zhi-Xin Hu, Jia-Song Wang, Ming-Chang Zhang, Han X. The inhibition of p38 MAPK blocked inflammation to restore the functions of rat meibomian gland epithelial cells. Experimental eye research. 2023-04-14. PMID:37059216. cell proliferation, keratinization, lipid accumulation, and matrix metalloproteinases 9 (mmp9) expression were evaluated by mtt assay, polymerase chain reaction (pcr), immunofluorescence staining, apoptosis assay, lipid staining, and western blot analyses. 2023-04-14 2023-08-14 mouse
E Qin, Shuojia Gu, Yimin Guo, Liyan Wang, Guimei P. MiRNA-30a-5p/VCAN Arrests Tumor Metastasis via Modulating the Adhesion of Lung Adenocarcinoma Cells. Applied biochemistry and biotechnology. 2023-04-09. PMID:37032373. several in vitro experiments were applied for cell function detection: dual-luciferase assay for validating the targeting relationship between mirna-30a-5p and its target gene; quantitative real-time polymerase chain reaction for testing the expression of mirna-30a-5p and its target gene in luad cells; mtt, transwell, cell adhesion, flow cytometry and immunofluorescence assays for examining the capabilities of luad cells to proliferate, migrate, invade, adhere, apoptosis and epithelial-mesenchymal transition (emt) effect; western blot for determining the expression of adhesion-related proteins and emt-related proteins. 2023-04-09 2023-08-14 Not clear
Der-An Tsao, Yu-Chien Wang, Shang-Tao Chien, Shih-Yi Lin, Huoy-Rou Chan. Fucoidan enhances the effect of chemotherapeutic drug against drug-resistant lung cancer cells. Pakistan journal of pharmaceutical sciences. vol 36. issue 1. 2023-03-27. PMID:36967501. cell culture, mtt assay, wound healing assay, gelatin zymography assay, dna fragmentation assay, apoptosis assay, reverse transcription polymerase chain reaction (rt-pcr) western blot analysis was adopted. 2023-03-27 2023-08-14 Not clear
Pedro Barrios-Bernal, José Lucio-Lozada, Maritza Ramos-Ramírez, Norma Hernández-Pedro, Oscar Arriet. A Novel Combination of Sotorasib and Metformin Enhances Cytotoxicity and Apoptosis in KRAS-Mutated Non-Small Cell Lung Cancer Cell Lines through MAPK and P70S6K Inhibition. International journal of molecular sciences. vol 24. issue 5. 2023-03-11. PMID:36901764. cellular cytotoxicity was evaluated by an mtt assay, apoptosis induction through flow cytometry, and mapk and mtor pathways were assessed by western blot. 2023-03-11 2023-08-14 Not clear
Xiao Han, Jiayan Huang, Jia Lu, Feng He, Fang Wang, Shuangfeng Li, Hao Zhong, Haiyang Yu, Weiling P. Active compounds and potential targets of Shuganning injection in the treatment of hepatocellular carcinoma by network pharmacology and in vitro validation. Naunyn-Schmiedeberg's archives of pharmacology. 2023-03-04. PMID:36869905. the in vitro test of the effects and mechanism of vanillin and baicalein was elucidated by mtt, western blot, immunofluorescence, and apoptosis analysis. 2023-03-04 2023-08-14 Not clear
Hongguang Sheng, Jun Xiong, Danying Yan. Protective Effect of Sevoflurane Preconditioning on Cardiomyocytes Against Hypoxia/Reoxygenation Injury by Modulating Iron Homeostasis and Ferroptosis. Cardiovascular toxicology. 2023-02-17. PMID:36800141. mtt was taken to assess the viability of each group of cells, flow cytometry to detect cell apoptosis, and qrt-pcr or western blot to detect the expression of iron metabolism-related proteins and apoptosis-related proteins in the cells. 2023-02-17 2023-08-14 rat
Rajeev Shrestha, Hyun Ji Lee, Junmo Lim, Pallavi Gurung, Til Bahadur Thapa Magar, Young-Tak Kim, Kija Lee, Seulgi Bae, Yong-Wan Ki. Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors. Life (Basel, Switzerland). vol 12. issue 12. 2022-12-23. PMID:36556469. cell proliferation and apoptosis were detected by the 4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) assay and western blot assay, respectively. 2022-12-23 2023-08-14 mouse