| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Rajeev Shrestha, Hyun Ji Lee, Junmo Lim, Pallavi Gurung, Til Bahadur Thapa Magar, Young-Tak Kim, Kija Lee, Seulgi Bae, Yong-Wan Ki. Effect of Photodynamic Therapy with Chlorin e6 on Canine Tumors. Life (Basel, Switzerland). vol 12. issue 12. 2022-12-23. PMID:36556469. |
cell proliferation and apoptosis were detected by the 4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) assay and western blot assay, respectively. |
2022-12-23 |
2023-08-14 |
mouse |
| Nan Zheng, Shan Liu, Huiting Zeng, Huajun Zhao, Lixu Ji. Molecular Mechanism of Curcumin Derivative on YAP Pathway against Ovarian Cancer. Journal of clinical medicine. vol 11. issue 23. 2022-12-11. PMID:36498794. |
mtt assay was used to detect the effect of wz10 on the proliferation of ovarian cancer ovcar3 cells; annexin v/pi double staining flow cytometry was used to detect the effect of wz10 on cell apoptosis; transwell method was used to detect the effect of wz10 on cell invasiveness; western blot was used to investigate the effect of wz10 mechanisms affecting ovcar3 activity in ovarian cancer in vitro. |
2022-12-11 |
2023-08-14 |
Not clear |
| Rui Zhang, Ziqi Meng, Xuwei Wu, Meihua Zhang, Zhengri Piao, Tiefeng Ji. PD-L1/p-STAT3 promotes the progression of NSCLC cells by regulating TAM polarization. Journal of cellular and molecular medicine. 2022-11-14. PMID:36372977. |
after constructing the co-culture system and conditioned medium system, the proliferation, apoptosis, metastasis, angiogenesis, emt process and stemness of nsclc were detected by mtt, flow cytometry, transwell, endothelial cell tube formation and western blot assays. |
2022-11-14 |
2023-08-14 |
Not clear |
| b' Seda \\xc5\\x9eirin, Hatice G\\xc3\\xbcl Dursun, Canan Ero\\xc4\\x9flu G\\xc3\\xbcne\\xc5\\x9. Composition analysis of Salsola grandis and its effects on colon cancer cells. Anti-cancer agents in medicinal chemistry. 2022-10-28. PMID:36305129.' |
we applied 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide, the thiazolyl blue (mtt) method to evaluate its effects on cell viability, elisa assay, real time pcr and western blot method to reveal its effects on apoptosis. |
2022-10-28 |
2023-08-14 |
Not clear |
| Aleksandra Jovanović Galović, Nataša Jovanović Lješković, Senka Vidović, Jelena Vladić, Nikola Jojić, Milan Ilić, Tatjana Srdić Rajić, Vesna Kojić, Dimitar Jakimo. The Effects of Resveratrol-Rich Extracts of Pharmaceutics. vol 14. issue 10. 2022-10-27. PMID:36297452. |
the cytotoxic effect is assessed by the mtt test and eb/ao staining, levels of apoptosis are determined by annexin v assay, autophagia by ulk-1 expression using western blot and nf-kb activation by p65 elisa. |
2022-10-27 |
2023-08-14 |
Not clear |
| Xianyan Wang, Hongmei Li, Ning Zhang, Tian Wu, Shaoqing Wan. FGFR2-CCDC6 fusion gene promotes the proliferation of Hucct-1 cells. Cellular and molecular biology (Noisy-le-Grand, France). vol 68. issue 6. 2022-10-13. PMID:36227665. |
the effect of the fusion gene on cell proliferation was examined by mtt and the expression of fgfr2/akt/signaling pathway proteins was detected by western blot. |
2022-10-13 |
2023-08-14 |
Not clear |
| Sicheng Chen, Mengjun Bie, Xiaowen Wang, Mengtian Fan, Bin Chen, Qiong Shi, Yingjiu Jian. PGRN exacerbates the progression of non-small cell lung cancer via PI3K/AKT/Bcl-2 antiapoptotic signaling. Genes & diseases. vol 9. issue 6. 2022-09-26. PMID:36157487. |
the proliferation, apoptosis, migration, and invasion of nsclc cells were assessed by the mtt assay, western blot, degree of wound healing, and transwell assays. |
2022-09-26 |
2023-08-14 |
mouse |
| Tao Zou, Shan Gao, Zhaolan Yu, Fuyong Zhang, Lan Yao, Mengyao Xu, Junxin Li, Zhigui Wu, Yilan Huang, Shurong Wan. Salvianolic acid B inhibits RAW264.7 cell polarization towards the M1 phenotype by inhibiting NF-κB and Akt/mTOR pathway activation. Scientific reports. vol 12. issue 1. 2022-08-16. PMID:35974091. |
the effects of different treatments on cell viability, gene expression and secretion of related proteins, phenotypic markers and cytokines were detected by mtt and western blot assays, rt‒qpcr and elisas. |
2022-08-16 |
2023-08-14 |
Not clear |
| Jiangqi Xu, Xiaohong Huang, Shixiang Liu, Dongdong Chen, Yufang Xie, Zhenwu Zha. The protective effects of lncRNA ZFAS1/miR-421-3p/MEF2C axis on cerebral ischemia-reperfusion injury. Cell cycle (Georgetown, Tex.). 2022-07-26. PMID:35880950. |
overexpressed or silenced plasmids of zfas1 were transfected into the cells to analyze the effects of oxygen-glucose deprivation/reperfusion (ogd/r) treatment on the viability, apoptosis and related gene expressions of neuro-2a cell by performing mtt assay, flow cytometry, qrt-pcr, and western blot. |
2022-07-26 |
2023-08-14 |
mouse |
| Yei-Jin Kang, Won-Geun Yang, Weon-Sik Chae, Dae-Won Kim, Seong-Gon Kim, Horatiu Rotar. Administration of 4‑hexylresorcinol increases p53‑mediated transcriptional activity in oral cancer cells with the p53 mutation. Oncology reports. vol 48. issue 3. 2022-07-20. PMID:35856441. |
to determine the effect of 4hr on p53 mutant carcinoma cells, western blot analysis, p53 transcriptional activity analysis, mtt assay and apoptosis immunocytochemistry were performed. |
2022-07-20 |
2023-08-14 |
human |
| Zhang Yang, Wang Yaling, Liu Tao, Rao Mingyue, Li Yongju. Cardioprotective effect of NRG-4 gene expression on spontaneous hypertension rats and its mechanism through mediating the activation of ErbB signaling pathway. Cellular and molecular biology (Noisy-le-Grand, France). vol 68. issue 1. 2022-07-09. PMID:35809324. |
quantitative real-time polymerase chain reaction (qrt-pcr) and western blot were used to detect the expression levels in addition, methyl thiazolyl tetrazolium salt (mtt) assay and flow cytometry were performed to detect the proliferation and apoptosis of cardiomyocytes, respectively. |
2022-07-09 |
2023-08-14 |
rat |
| Xin Zhang, Xuebing Zhou, Ming Gao, You Lyu, Ying Wang, Chunyu Yang, Yingshi Piao, Xiangshan Re. [Cordycepin inhibits the proliferation and migration of human gastric cancer cells by suppressing lipid metabolism via AMPK and MAPK activation]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 38. issue 6. 2022-06-22. PMID:35732609. |
the proliferation ability of mgc-803 and hgc-27 cells was detected by mtt assay and edu assay; the colony formation ability of cells was detected by colony formation assay; both wound healing assay and cell migration assay were applied to detect the cell migration ability of mgc-803 and hgc-27 cells; the chromatin agglutination was detected by hoechst 33342 staining; the apoptosis of gastric cancer cells was detected by annexin v-fitc/pi double labeling combined with flow cytometry; western blot was used to measure the protein expression levels of lipid metabolism-related proteins including sterol regulatory element binding transcription factor 1 (srebf1), fatty acid synthase (fasn), and acetyl coa carboxylase 1 (acc1), epithelial-mesenchymal transition (emt)-related proteins including e-cadherin, vimentin, snail, slug, matrix metalloproteinase 2 (mmp2), mmp9, ampk, and phosphorylated ampk (p-ampk), mapk signaling pathway-related proteins including jnk, phosphorylated jnk (p-jnk), p38 mapk, and p-p38 mapk, and apoptosis-related proteins including cleaved caspase-9 (c-caspase-9), c-caspase-3, and cleaved poly (adp-ribose) polymerase (c-parp). |
2022-06-22 |
2023-08-14 |
human |
| Yanghui Huang, Guangyu Zhen. Circ_UBE2D2 Attenuates the Progression of Septic Acute Kidney Injury in Rats by Targeting miR-370-3p/NR4A3 Axis. Journal of microbiology and biotechnology. vol 32. issue 6. 2022-06-20. PMID:35722711. |
after sh-circ_ube2d2 was transfected into cells, the transfection efficiency was detected by qrt-pcr, cell viability and apoptosis were determined by mtt assay and flow cytometry, and expressions of bcl-2, bax and cleaved-caspase 3 were quantified by western blot. |
2022-06-20 |
2023-08-14 |
rat |
| Zhiguo Wu, Jian Tan, Lichang Lin, Wenting Zhang, Wanqiu Yua. microRNA-140-3p protects hippocampal neuron against pyroptosis to attenuate sevoflurane inhalation-induced post-operative cognitive dysfunction in rats via activation of HTR2A/ERK/Nrf2 axis by targeting DNMT1. Cell death discovery. vol 8. issue 1. 2022-06-16. PMID:35710537. |
in neurons, cell viability, apoptosis, and pyroptosis-related factors were tested by mtt, flow cytometry, and western blot analysis respectively. |
2022-06-16 |
2023-08-14 |
rat |
| Ilan Zaffran, Nadine Landolina, Pratibha Gaur, Tihana Lenac Rovis, Jonjic Stipan, Ofer Mandelboim, Bernhard B Singer, Francesca Levi-Schaffe. Activation of CEACAM1 with an agonistic monoclonal antibody results in inhibition of melanoma cells. Cancer gene therapy. 2022-06-10. PMID:35681020. |
using this mab, we assessed the expression of ceacam1 on four different human melanoma cell lines by western blot and flow cytometry and determined its effect on cell viability in vitro by mtt assay. |
2022-06-10 |
2023-08-14 |
mouse |
| Xuan Zou, Kaitao Yu, Xiaoyang Chu, Lili Yan. Betanin alleviates inflammation and ameliorates apoptosis on human oral squamous cancer cells SCC131 and SCC4 through the NF-κB/PI3K/Akt signaling pathway. Journal of biochemical and molecular toxicology. 2022-06-01. PMID:35645143. |
the cytotoxicity assay, intracellular ros, mmp, cell apoptosis, and inflammatory mediators of betanin activity on scc131 and scc4 cells were evaluated by mtt assay, dcfh-da, rh-123, ao/eb, dapi, pi, analysis of western blot and rt-pcr. |
2022-06-01 |
2023-08-13 |
human |
| Song Qi, Li Xu, Yongyuan Han, Hongkun Chen, Anyuan Chen. miR-29a-3p mitigates the development of osteosarcoma through modulating IGF1 mediated PI3k/Akt/FOXO3 pathway by activating autophagy. Cell cycle (Georgetown, Tex.). 2022-05-16. PMID:35575588. |
then, the 3- (4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (mtt) assay, colony formation experiment, western blot and transwell assay were conducted to validate os cell proliferation, colony formation ability, apoptosis, migration and invasion. |
2022-05-16 |
2023-08-13 |
Not clear |
| Lin Liu, Tingting Zhou, Tao Li, Zhanhua Liang, Xiaoguang Lu. LncRNA DLX6-AS1 promotes microglial inflammatory response in Parkinson's disease by regulating the miR-223-3p/NRP1 axis. Behavioural brain research. 2022-05-13. PMID:35550840. |
meanwhile, the cell number of th+/caspase3+/iba1+ in substantia nigra, cell viability, and apoptosis rate of bv2 microglia, inflammatory levels, and nlr family pyrin domain containing 3 (nlrp3) inflammasome were determined using immunohistochemistry, mtt assay, flow cytometry, elisa assay, and western blot. |
2022-05-13 |
2023-08-13 |
mouse |
| Dingmei Deng, Xian Ye, Xiyue Wang, Guangning H. Retracted Article: Gemcitabine aggravates miR-199a-5p-mediated breast cancer cell apoptosis by promoting VEGFA downregulation RSC advances. vol 9. issue 35. 2022-05-06. PMID:35514680. |
functional experiments were performed to explore cell viability, apoptosis rate, and expressions of apoptosis-related genes: cell viability was assessed by mtt staining, apoptosis rate was recorded by flow cytometry, and western blot was used to evaluate the expressions of bcl-2, bax and cleaved caspase 3. |
2022-05-06 |
2023-08-13 |
Not clear |
| Ye Cao, Jiajia Zheng, Chentao L. Retracted Article: miR-199a-3p knockdown inhibits dedifferentiated liposarcoma (DDLPS) cell viability and enhances apoptosis through targeting casein kinase-1 alpha (CK1α). RSC advances. vol 9. issue 39. 2022-05-06. PMID:35519458. |
functionally, mtt assay, flow cytometry and western blot results demonstrated that knockdown of mir-199a-3p inhibited ddlps cell viability, enhanced apoptosis rate, and decreased expression of apoptosis-related genes bax and cleaved caspase 3, as well as increased bcl-2 expression |
2022-05-06 |
2023-08-13 |
Not clear |