All Relations between blot and mtt

Publication Sentence Publish Date Extraction Date Species
Shuyan Sheng, Wenzhuo Su, Deshen Mao, Conghan Li, Xinyang Hu, Wanyu Deng, Yong Yao, Yongsheng J. MicroRNA-21 induces cisplatin resistance in head and neck squamous cell carcinoma. PloS one. vol 17. issue 4. 2022-04-14. PMID:35421166. reverse transcription-pcr to detect expression, mtt and clonogenic assays to evaluate cell proliferation, apoptosis assays, resazurin cell viability assays, western blot and luciferase reporter assays to detect protein expression, and flow cytometry to analyse the cell cycle were adopted. 2022-04-14 2023-08-13 Not clear
Shaosheng Liu, Yuanyuan Li, Zhaoxia L. Salidroside suppresses the activation of nasopharyngeal carcinoma cells via targeting miR-4262/GRP78 axis. Cell cycle (Georgetown, Tex.). 2022-02-28. PMID:35220889. npc cells were cultured, mtt was used to detect the effect of salidroside on cell proliferation, apoptosis detected by flow cytometry assay, western blot was used to detect the related protein expression. 2022-02-28 2023-08-13 mouse
Lei Zou, Cuifen Shi, Dawei Wang, Juan Cheng, Qi Wang, Lei Wang, Guoya Yan. Long non-coding RNA-non-coding RNA activated by DNA damage inhibition suppresses hepatic stellate cell activation via microRNA-495-3p/sphingosine 1-phosphate receptor 3 axis. Bioengineered. vol 13. issue 3. 2022-02-24. PMID:35200103. the effects of pdgf-bb on lx-2 cell viability, migration, invasion, and apoptosis were evaluated using mtt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), transwell, flow cytometry, and western blot assays. 2022-02-24 2023-08-13 Not clear
T Tuerxun, X Li, F Lou, X Wang, L M. YBX1 Protects against Apoptosis Induced by Oxygen-Glucose Deprivation/Reoxygenation in PC12 Cells via Activation of the AKT/GSK3β Pathway. Folia biologica. vol 67. issue 4. 2022-02-12. PMID:35151240. next, the effect of ybx1 on the apoptosis and viability of ogd/r-treated pc12 cells was evaluated by flow cytometry, mtt assay, and western blot. 2022-02-12 2023-08-13 cat
Mansoureh Hashemi, Aida Abbasiazam, Saeed Oraee-Yazdani, Janice Lenze. Response of human glioblastoma cells to hyperthermia: Cellular apoptosis and molecular events. Tissue & cell. vol 75. 2022-02-10. PMID:35144007. cell survival rate by mtt assay and apoptosis using quantitative real-time rt-pcr and western blot were evaluated. 2022-02-10 2023-08-13 human
Hao Zha, Wei Miao, Wei Rong, Aimei Wang, Weiqi Jiang, Rui Liu, Lanqi Liu, Ying Wan. Remote ischaemic perconditioning reduces the infarct volume and improves the neurological function of acute ischaemic stroke partially through the miR-153-5p/TLR4/p65/IkBa signalling pathway. Folia neuropathologica. vol 59. issue 4. 2022-02-04. PMID:35114774. overexpressed or silenced mir-153-5p was transfected into the cells to analyse the effects of oxygen-glucose deprivation (ogd) treatment on neuro-2a cell viability, apoptosis, and related gene expressions by performing quantitative real-time polymerase chain reaction (qrt-pcr), mtt assay, flow cytometry, and western blot. 2022-02-04 2023-08-13 mouse
Xiaoling Zhang, Si Wang, Yuyan Cai, Weihong He, Qing Yang, Chen L. Regulatory Mechanism of MicroRNA-9 / Long Non-Coding RNA XIST Expression on Mouse Macrophage RAW264.7 Apoptosis Induced by Oxidized Low Density Lipoprotein. Bioengineered. vol 13. issue 2. 2022-02-03. PMID:35109760. methylthiazolyl tetrazolium (mtt) method, flow cytometry, and western blot were used to detect and compare the differences of cell proliferation, apoptosis, and the expression levels of apoptosis signal-regulating kinase 1 (ask1), c-jun n-terminal kinase (jnk), matrix metalloproteinase-9 (mmp-9), and b-cell lymphoma-2 (bcl-2) after ox-ldl induction and transfection of mir-9 mimic, mir-9 inhibitor and xist expression vector, respectively. 2022-02-03 2023-08-13 mouse
Xiaoxiao Tao, Yaping Jiang, Xian Zheng, Xiaoxiao Ji, Feifei Pen. Dihydromyricetin ameliorates oxygen‑glucose deprivation and re‑oxygenation‑induced injury in HT22 cells by activating the Wnt/β‑catenin signaling pathway. Molecular medicine reports. vol 25. issue 3. 2022-01-28. PMID:35088876. the effects of dmy on the viability, oxidative stress and apoptosis of ht22 cells following oxygen‑glucose deprivation and re‑oxygenation (ogd/r) were examined using mtt, lactate dehydrogenase (ldh), superoxide (sod), malondialdehyde (mda), western blot and tunel assays. 2022-01-28 2023-08-13 Not clear
Yixia Zhang, Xindan Meng, Keyu Li. The modulation of cAMP/PKA pathway by asiaticoside ameliorates high glucose-induced inflammation and apoptosis of retinal pigment epithelial cells. Journal of bioenergetics and biomembranes. 2022-01-17. PMID:35038080. then, cell survival rate, expression of inflammatory factors, oxidative stress, and apoptosis were measured by mtt method, western blot, oxidative stress detection kits and tunel respectively. 2022-01-17 2023-08-13 human
Lekshmi Krishna, Sagar Nilawar, Murugeswari Ponnalagu, Murali Subramani, Chaitra Jayadev, Rohit Shetty, Kaushik Chatterjee, Debashish Da. Fiber Diameter Differentially Regulates Function of Retinal Pigment and Corneal Epithelial Cells on Nanofibrous Tissue Scaffolds. ACS applied bio materials. vol 3. issue 2. 2022-01-12. PMID:35019286. the molecular changes in the cells in response to the different fibers were analyzed by qrt-pcr, western blot, immunofluorescence, elisa, flow cytometry, mtt assay, and sem to assess properties such as proliferation, apoptosis, membrane potential, epithelial-mesenchymal transition, stem cell population, vegf-a secretion, differentiation, and metabolic status of the cells. 2022-01-12 2023-08-13 human
Xin-Yu Lu, Zhong-Ze Wang, Si-Cheng Wan, Er-Hu Zhao, Hong-Juan Cu. [Effects of tetrandrine on proliferation, migration, and invasion of glioblastoma cells]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 46. issue 24. 2022-01-07. PMID:34994145. in this study, mtt and brdu assays were employed to detect the effect of tet on the proliferation of ln229 glioblastoma cells; flow cytometry was used to analyze the cycle distribution and apoptosis; plate cloning assay and soft agar colony formation assay were performed to study the colony formation ability of ln229 cells exposed to tet; scratch assay and transwell assay were conducted to detect the ability of migration and invasion; western blot was adopted to the exploration of the molecular mechanism. 2022-01-07 2023-08-13 Not clear
Dan Liu, Ye Cheng, Zhipeng Tang, Junliang Chen, Ying Xia, Chengbin Xu, Xiangyu Ca. Potential mechanisms of methylglyoxal-induced human embryonic kidney cells damage: Regulation of oxidative stress, DNA damage, and apoptosis. Chemistry & biodiversity. 2021-12-28. PMID:34962083. cell viability, apoptosis and the signaling pathways were measured with mtt, fluorescent staining and western blot experiments, the results showed that mgo could induce oxidative stress and cell inflammation, the level of reactive oxygen species (ros) increased, and p38mapk, jnk and nf-κb signaling pathways were activated. 2021-12-28 2023-08-13 human
Jin Zou, Zi-Lin Wan, Lan Yao, Meng-Yao Xu, Shu-Rong Wan. [Rhein inhibits hydrogen peroxide-induced apoptosis of human umbilical vein endothelial cells and its mechanism]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 46. issue 23. 2021-12-24. PMID:34951247. cell survival rate was detected by mtt assay, apoptosis by annexin v-fitc/pi double staining and hoechst 33258 fluorescence staining, autophagy by ad-mcherry-gfp-lc3 b adenovirus transfection, and protein expression by western blot. 2021-12-24 2023-08-13 human
Shuang Liu, Wen Yan, Yanbing Hu, Huiying W. Shikonin Alleviates Endothelial Cell Injury Induced by ox-LDL via AMPK/Nrf2/HO-1 Signaling Pathway. Evidence-based complementary and alternative medicine : eCAM. vol 2021. 2021-12-16. PMID:34912465. the mtt method was used to detect cell viability, flow cytometry was used to detect cell apoptosis and reactive oxygen species (ros) levels, and western blot was used to detect protein levels. 2021-12-16 2023-08-13 human
Sahar Sadegh-Nejadi, Reza Afrisham, Solaleh Emamgholipour, Pantea Izadi, Neda Eivazi, Behnoosh Tahbazlahafi, Maliheh Pakneja. Influence of plasma circulating exosomes obtained from obese women on tumorigenesis and tamoxifen resistance in MCF-7 cells. IUBMB life. vol 72. issue 9. 2021-11-22. PMID:32542981. for evaluating resistance to tamoxifen, the cell viability, apoptosis, and the p53 protein were evaluated using the mtt assay, flow cytometry, and western blot methods, respectively. 2021-11-22 2023-08-13 Not clear
Xiangyu Cao, Chengying Liu, Mengmeng Zhang, Ruochen Bi, Mingyang Fu, Elena Korik, Jiahe Chen, Jianyun Gao, Igor Semak, Jianli Li. Bovine lactoferrin and Lentinus edodes mycelia polysaccharide complex: The formation and the activity to protect islet β cells. International journal of biological macromolecules. vol 191. 2021-11-11. PMID:34592222. cell viability, reactive oxygen species (ros) level, apoptosis and the related signaling pathways were detected with thiazolyl blue tetrazolium bromide (mtt) assay, 2,7-dichlorofluorescin diacetate (dcfh-da) staining, hoechst 33258 staining and western blot methods respectively. 2021-11-11 2023-08-13 cattle
Wei Yang, Yunbin Chen, Chao Huang, Wenjian Wang, Congfu Huang, Yuanguang L. MiR-18a Inhibits PI3K/AKT Signaling Pathway to Regulate PDGF BB-Induced Airway Smooth Muscle Cell Proliferation and Phenotypic Transformation. Physiological research. vol 70. issue 6. 2021-10-30. PMID:34717064. cell proliferation, apoptosis and migration were detected by mtt, flow cytometry and transwell, respectively; the expression of contractile phenotype marker proteins (sm-22alpha, alpha-sm-actin, calponin) and key molecules of the phosphatidylinositol 3-kinase (pi3k)/akt pathway (pi3k, p-pi3k, akt and p-akt) in asmcs were detected by western blot. 2021-10-30 2023-08-13 human
Natalia Magdalena Lisiak, Izabela Lewicka, Mariusz Kaczmarek, Jacek Kujawski, Barbara Bednarczyk-Cwynar, Lucjusz Zaprutko, Blazej Rubi. Oleanolic Acid's Semisynthetic Derivatives HIMOXOL and Br-HIMOLID Show Proautophagic Potential and Inhibit Migration of HER2-Positive Breast Cancer Cells In Vitro. International journal of molecular sciences. vol 22. issue 20. 2021-10-26. PMID:34681931. viability tests, cell cycle assessment, evaluation of apoptosis, autophagy, and adhesion/migration processes were performed using mtt, clonogenic, cytofluorometry, western blot, and qpcr. 2021-10-26 2023-08-13 human
Jing Xu, Jinglin Xi. NRP-1 silencing suppresses hepatocellular carcinoma cell growth in vitro and in vivo. Experimental and therapeutic medicine. vol 5. issue 1. 2021-10-21. PMID:23251257. protein and mrna expression of nrp-1 were examined by western blot analysis and quantitative reverse transcription-polymerase chain reaction (rt-pcr), and the effect of the lentivirus on cell growth was determined using mtt assay. 2021-10-21 2023-08-12 mouse
Jie Lin, Tieliang Ma, Xiaodong Jiang, Zhijun Ge, Weiliang Ding, Yuanyuan Wu, Guojun Jiang, Jiake Feng, Guoxing Cui, Yongfei Ta. JWA regulates human esophageal squamous cell carcinoma and human esophageal cells through different mitogen-activated protein kinase signaling pathways. Experimental and therapeutic medicine. vol 7. issue 6. 2021-10-21. PMID:24926382. western blot analysis was conducted to observe the effect of jwa on apoptosis and the regulatory effect of jwa on proliferation was determined using a thiazolyl blue tetrazolium bromide (mtt) assay. 2021-10-21 2023-08-13 human