| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Jie Lin, Tieliang Ma, Xiaodong Jiang, Zhijun Ge, Weiliang Ding, Yuanyuan Wu, Guojun Jiang, Jiake Feng, Guoxing Cui, Yongfei Ta. JWA regulates human esophageal squamous cell carcinoma and human esophageal cells through different mitogen-activated protein kinase signaling pathways. Experimental and therapeutic medicine. vol 7. issue 6. 2021-10-21. PMID:24926382. |
western blot analysis was conducted to observe the effect of jwa on apoptosis and the regulatory effect of jwa on proliferation was determined using a thiazolyl blue tetrazolium bromide (mtt) assay. |
2021-10-21 |
2023-08-13 |
human |
| Lianggeng Xiong, Binxin Tan, Xiubing Lei, Biao Zhang, Wenting Li, Daimei Liu, Tian Xi. SIRT6 through PI3K/Akt/mTOR signaling pathway to enhance radiosensitivity of non-Small cell lung cancer and inhibit tumor progression. IUBMB life. vol 73. issue 9. 2021-10-15. PMID:34033225. |
then, check the cell proliferation, migration, apoptosis and cell cycle by mtt, wound healing assay and flow cytometry, while detect the expression of pi3k/akt/mtor signaling pathway-related proteins by western blot. |
2021-10-15 |
2023-08-13 |
mouse |
| Liu-Kun Yang, Liang Lu, Jiao Yue, Xin-Shang Wang, Jing-Yu Qi, Fan Yang, Shui-Bing Li. Activation of microglial G‑protein-coupled receptor 30 protects neurons against excitotoxicity through NF-κB/MAPK pathways. Brain research bulletin. vol 172. 2021-10-07. PMID:33848615. |
in this study, mtt, western blot, immunocytochemical staining, phagocytosis assay and wound healing assay were employed to detect the effect of gpr30 in n9 microglial cells after exposure to glutamate. |
2021-10-07 |
2023-08-13 |
Not clear |
| Bingwu Liu, Xinfeng Yan, Zuojia Hou, Lei Zhang, Duwen Zhan. Impact of Bupivacaine on malignant proliferation, apoptosis and autophagy of human colorectal cancer SW480 cells through regulating NF-κB signaling path. Bioengineered. vol 12. issue 1. 2021-09-21. PMID:34151717. |
our work treated crc cells with bupivacaine, detected cell vitality through mtt assay, apoptosis through flow cytometry, cell migration through wound healing assay, nf-κb activity through immunofluorescence, inflammatory factor level, including tnf-α, il-1β as well as il-6 through eslia, apoptosis factor mrna expression, including bcl-2, bax and caspase-3q through qrt-pcr, and protein expression linking with nf-κb signaling pathway as well as autophagy-related proteins via western blot. |
2021-09-21 |
2023-08-13 |
human |
| Jingyun Zhang, Liu Yang, Xiaoxiao Han, Chenchi Li, Ranyang Liu, Zihua Ma, Bing Han, Rujia Xie, Qin Yan. [Endoplasmic reticulum stress in hepatic stellate cells induced by tunicamycin promotes apoptosis and cell cycle arrest]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 37. issue 9. 2021-09-20. PMID:34533126. |
hsc-t6 cells were randomized into control group, treatment group with 1 ml/l of dimethyl sulfoxide (dmso), and treatment group with 1 μg/ml of tunicamycin, and the cells were treated for 12 h. mtt assay was used to detect cell proliferation, flow cytometry to detect apoptosis and cell cycle, and western blot to detect the protein expressions of α-smooth muscle actin (α-sma), c/ebp camp homologous protein (chop), caspase-12, and cyclin d1. |
2021-09-20 |
2023-08-13 |
rat |
| Yuan Wang, Huazhang Wu, Nannan Dong, Xu Su, Mingxiu Duan, Yaqin Wei, Jun Wei, Gaofeng Liu, Qingjie Peng, Yunli Zha. Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells. Scientific reports. vol 11. issue 1. 2021-09-17. PMID:33510228. |
in the present study, we investigate the effects of sfn on cell proliferation, cell cycle, cell apoptosis, and also the expression of several cell cycle and apoptosis-related genes by mtt assay, flow cytometry and western blot analysis in gastric cancer (gc) cells. |
2021-09-17 |
2023-08-13 |
Not clear |
| Ke Liu, Jiang-Dong Ni, Wen-Zhao Li, Bai-Qi Pan, Yu-Ting Yang, Qin Xia, Jun Huan. The Sp1/FOXC1/HOTTIP/LATS2/YAP/β-catenin cascade promotes malignant and metastatic progression of osteosarcoma. Molecular oncology. vol 14. issue 10. 2021-09-10. PMID:32634265. |
the in vitro effects of foxc1 or hottip on cell viability, proliferation, migration, invasion, and expression of target genes were examined using mtt, colony-forming assay, wound-healing, transwell invasion, and western blot, respectively; the in vivo effects were examined using xenograft and experimental metastasis models. |
2021-09-10 |
2023-08-13 |
human |
| Man-Lin Guo, Xiang-Yu Ma, Yu-Qing Gong, Meng-Lin Feng, Yu-Wan Zhao, Leng-Xin Dua. [Protective effect of ethyl acetate extract from Bidens bipinnata on hepatocyte damage induced by endoplasmic reticulum stress]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 46. issue 15. 2021-09-03. PMID:34472265. |
methyl thiazolyl tetrazolium(mtt) colorimetric assay was used to investigate the survival rate of ethyl acetate extract from b. bipinnata in l02 cells injury induced by endoplasmic reticulum stress; the protein expressions of endoplasmic reticulum stress-related molecule glucose regulated protein 78(grp78), pkr-like er kinase(perk), eukaryotic initiation factor-2(eif2α), activating transcription factor 4(atf4), c/ebp homologous protein(chop), b-cell cll/lymphoma 2(bcl-2), bal-2 associated x apoptosis regulator(bax) were examined by wes-tern blot. |
2021-09-03 |
2023-08-13 |
Not clear |
| Yuangui Zhang, Qingqing Sun, Aixia Fan, Guimin Don. Isoflurane triggers the acute cognitive impairment of aged rats by damaging hippocampal neurons via the NR2B/CaMKII/CREB pathway. Behavioural brain research. vol 405. 2021-09-02. PMID:33636236. |
isoflurane also decreased the viabilities and strengthened the apoptotic potential of hippocampal neurons by damaging the mitochondria in a time-dependent manner compared with the control group which was reported by mtt, immunofluorescent assay, flow cytometry and western blot assay in vitro. |
2021-09-02 |
2023-08-13 |
rat |
| Wenjing Li, Feifei Wang, Fusheng Dong, Zhiyong Zhang, Peng Song, Huizhen Chen, Jie Wan. CGF Membrane Promotes Periodontal Tissue Regeneration Mediated by hUCMSCs through Upregulating TAZ and Osteogenic Differentiation Genes. Stem cells international. vol 2021. 2021-08-17. PMID:34394357. |
the effects of the extracted cgf membrane on cell viability, osteogenic differentiation ability, osteopontin (opn) expression, alkaline phosphatase (alp) content, and osteogenic differentiation-related genes (runt-related transcription factor 2 (runx2); osteocalcin (ocn); alp), tafazzin (taz) expression, and nuclear transfer were examined by mtt assay, alizarin red staining, immunofluorescence, enzyme-linked immunosorbent assay (elisa), quantitative real-time polymerase chain reaction (qrt-pcr), and western blot. |
2021-08-17 |
2023-08-13 |
human |
| Xiaohuan Ding, Jing Lv, Jia Luan, Jun Zhan. Calycosin may Alleviate Ang II-Induced Pro-proliferative Effects on Glomerular Mesangial Cells via Partially Inhibiting Autophagy and ERK Signaling Pathway. Biological & pharmaceutical bulletin. vol 43. issue 12. 2021-08-16. PMID:32999213. |
human glomerular mesangial cells (gmcs) were cultured and treated by ang ii and 0, 0.1, 1, or 10 µm calycosin, and the viability and proliferation of the cells were determined by methyl thiazolyl tetrazolium (mtt) and 5-ethynil-2'-deoxyuridine (edu) staining; moreover, the apoptosis of the cells was examined by flow cytometry assay; furthermore, the expression levels of extracellular signal-regulated kinase (erk), p-erk, anti-apoptotic factor bcl-2, as well as pro-apoptotic factor bax have been examined by western blot (wb) methods; finally, the expression of autophagic markers in each group was examined by wb and immunocytochemistry methods. |
2021-08-16 |
2023-08-13 |
human |
| Yifan Chen, Min Zhang, Xinghao Ji, Jinjin Zhao, Shuang Qin, Yinghua Ji, Ruijuan Fan, Yanting Liu, Ping L. The anti-cancer effect of metoclopramide on triple-negative breast cancer cells. Die Pharmazie. vol 76. issue 4. 2021-08-13. PMID:33849703. |
this study aimed at elucidating the effect of metoclopramide on triple-negative breast cancer, mda-mb-231 cells were treated with various concentrations of metoclopramide, the cell proliferation was detected by mtt method, the apoptosis rate was detected by annexin v/pi double staining method, the expression change of death-related protein was detected by western blot. |
2021-08-13 |
2023-08-13 |
Not clear |
| Rong Ding, Wenyue Kang, Duozhi Wu, Lin Wan. Protective effect of propofol via the regulation of ovarian granulosa cell proliferation and apoptosis. Experimental and therapeutic medicine. vol 22. issue 3. 2021-08-06. PMID:34345270. |
kgn cells were transfected with mir-451a inhibitor or inhibitor control sequences for 6 h and treated with 10 µg/ml propofol for an additional 48 h. the results from the mtt, rt-qpcr and western blot assays indicated that 10 µg/ml propofol inhibited cell viability, induced apoptosis, enhanced cleaved caspase 3 expression, reduced pro-caspase 3 levels and inhibited the protein and mrna expression of wnt3a and β-catenin. |
2021-08-06 |
2023-08-13 |
human |
| Degui Shu, Yufen Xu, Wenyu Che. Knockdown of lncRNA BLACAT1 reverses the resistance of afatinib to non-small cell lung cancer via modulating STAT3 signalling. Journal of drug targeting. vol 28. issue 3. 2021-07-16. PMID:31359792. |
mtt assay, colony formation assay, apoptosis analysis, qrt-pcr and western blot analysis, immunohistochemistry, and in vivo study were carried out. |
2021-07-16 |
2023-08-13 |
Not clear |
| Shu Wang, Yuqing Huang, Guangyan Luo, Xin Yang, Wei Huan. Cyanidin-3-O-glucoside attenuates high glucose-induced podocyte dysfunction by inhibiting apoptosis and promoting autophagy via activation of SIRT1/AMPK pathway. Canadian journal of physiology and pharmacology. vol 99. issue 6. 2021-06-22. PMID:33049148. |
mtt, flow cytometry assay, and western blot analysis showed that c3g could reverse the increase of cell apoptosis under high glucose treatment in mpc5 cells by upregulation of |
2021-06-22 |
2023-08-13 |
Not clear |
| Wensi Chen, Shiping He, Daoman Xian. Hypoxia-induced retinal pigment epithelium cell-derived bFGF promotes the migration and angiogenesis of HUVECs through regulating TGF-β1/smad2/3 pathway. Gene. vol 790. 2021-06-07. PMID:33964379. |
after cell culture in hypoxia conditions, the cell viability, apoptosis, and the expressions of bfgf and vascular endothelial growth factor a (vegfa) in human rpes were detected by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt), flow cytometry, western blot, rt-qpcr, or elisa. |
2021-06-07 |
2023-08-13 |
human |
| Aihua Liu, Zhongfu Zuo, Linlin Liu, Lihua Li. Down-regulation of NTSR3 inhibits cell growth and metastasis, as well as the PI3K-AKT and MAPK signaling pathways in colorectal cancer. Biochemistry and cell biology = Biochimie et biologie cellulaire. vol 98. issue 5. 2021-05-20. PMID:32125883. |
western blot analysis, real-time pcr, immunofluorescence staining, mtt, cell cycle assay, cell apoptosis assay, hoechst staining, caspase-3 and caspase-9 activity assays, cell adhesion assay, wound healing assay, and a transwell assay were used in this study. |
2021-05-20 |
2023-08-13 |
Not clear |
| Xiaomei Du, Zhiwen Xue, Jianning Lv, Heidou Wan. Expression of the Topoisomerase II Alpha (TOP2A) Gene in Lung Adenocarcinoma Cells and the Association with Patient Outcomes. Medical science monitor : international medical journal of experimental and clinical research. vol 26. 2021-05-20. PMID:33361736. |
for a further elucidation of the value of top2a in luad, the effect of top2a knockout on cell viability and related protein expression of luad cell line a549 in vitro was investigated by using rna interference, mtt, flow cytometry, rt-pcr, and western blot analysis. |
2021-05-20 |
2023-08-13 |
human |
| Zeinab Mohseni, Hamid Sedighian, Raheleh Halabian, Jafar Amani, Elham Behzadi, Abbas Ali Imani Foolad. Potent in vitro antitumor activity of B-subunit of Shiga toxin conjugated to the diphtheria toxin against breast cancer. European journal of pharmacology. vol 899. 2021-05-17. PMID:33753109. |
t47d, skbr3, 4t1 and mcf7 cell lines were treated separately with purified dt389-stxb recombinant protein and functional activity of dt389-stxb was analyzed by the cell enzyme-linked immunosorbentassay (elisa), mtt, icc, western blot and apoptosis tests. |
2021-05-17 |
2023-08-13 |
human |
| Xiang Zhang, Jiong-Ming You, Xiao-Jun Dong, Yang W. Administration of mircoRNA-135b-reinforced exosomes derived from MSCs ameliorates glucocorticoid-induced osteonecrosis of femoral head (ONFH) in rats. Journal of cellular and molecular medicine. vol 24. issue 23. 2021-05-12. PMID:33089961. |
real-time pcr, western blot analysis, ihc assay, tunel assay, mtt assay and flow cytometry were performed to detect the effect of hips-msc-exos and mir-135b on cell apoptosis and the expression of pdcd4/caspase-3/ocn. |
2021-05-12 |
2023-08-13 |
rat |