Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
Neeshu Saroj, Shiv Shanker, Eduardo Serrano-Hernández, Gabriel Manjarrez-Gutiérrez, José-Antonio Mondragón, Saidel Moreno-Martínez, Rosa A Jarillo-Luna, Pedro López-Sánchez, José A Terró. Expression of tryptophan hydroxylase in rat adrenal glands: Upregulation of TPH2 by chronic stress. Psychoneuroendocrinology. vol 171. 2024-10-28. PMID:39467477. |
on day 15, adrenal glands were collected for tph1 and tph2 immunohistochemistry, western blot, and rt-pcr; tph activity was estimated by quantification of 5-hydroxytryptophan (5-htp) and, indirectly, through measurement of 5-ht and 5-hydroxindolacetic acid (5-hiaa) levels and turnover (5-hiaa/5-ht ratio) by hplc. |
2024-10-28 |
2024-10-31 |
rat |
Yu-Yao Zou, Xiao-Yu Wu, Lan Shu, Pei-Lin Ji, Hua-Shan Gong, Wei-Fang Ron. [Perinatal antibiotics exposure causes increase in serum 5-hydroxytryptamine level as well as changes in behavior and gastrointestinal motility in the male offspring in mice]. Sheng li xue bao : [Acta physiologica Sinica]. vol 72. issue 3. 2020-09-24. PMID:32572427. |
serum 5-ht levels were detected with elisa, and potassium/sodium hyperpolarization activated cyclic nucleotide-gated channel 2 (hcn2) and tryptophan hydroxylase 1 (tph1) expression levels in colon epithelium of offspring were detected by western blot and rt-qpcr. |
2020-09-24 |
2023-08-13 |
mouse |
F Jiménez-Trejo, I Coronado-Mares, M Boeta, I González-Santoyo, R Vigueras-Villaseñor, C Arriaga-Canon, L A Herrera, M Tapia-Rodrígue. Identification of serotoninergic system components in stallion sperm. Histology and histopathology. vol 33. issue 9. 2019-01-10. PMID:29658099. |
therefore, using both immunofluorescence and western blot techniques, we searched for and found some serotonin markers such as 5-ht, 5-ht1b, 5-ht2a, 5-ht₃ receptors, both tph1 and maoa enzymes, and serotonin transporter (5-htt) in stallion sperm. |
2019-01-10 |
2023-08-13 |
Not clear |
Francisco Jiménez-Trejo, Miguel Tapia-Rodríguez, Marco Cerbón, Donald M Kuhn, Gabriel Manjarrez-Gutiérrez, C Adriana Mendoza-Rodríguez, Ofir Picaz. Evidence of 5-HT components in human sperm: implications for protein tyrosine phosphorylation and the physiology of motility. Reproduction (Cambridge, England). vol 144. issue 6. 2013-04-26. PMID:23028123. |
we used both immunocytochemistry and western blot to detect serotoninergic markers such as 5-ht, tph1, mao(a), 5-ht(1b), 5-ht(3), and 5ht(t); hplc for tph enzymatic activity; computer assisted semen analysis assays to measure sperm motility parameters and pharmacological approaches to show the effect of 5-ht in sperm motility and tyrosine phosphorylation was assessed by western blot. |
2013-04-26 |
2023-08-12 |
human |