All Relations between blot and serotonin

Publication Sentence Publish Date Extraction Date Species
Neeshu Saroj, Shiv Shanker, Eduardo Serrano-Hernández, Gabriel Manjarrez-Gutiérrez, José-Antonio Mondragón, Saidel Moreno-Martínez, Rosa A Jarillo-Luna, Pedro López-Sánchez, José A Terró. Expression of tryptophan hydroxylase in rat adrenal glands: Upregulation of TPH2 by chronic stress. Psychoneuroendocrinology. vol 171. 2024-10-28. PMID:39467477. on day 15, adrenal glands were collected for tph1 and tph2 immunohistochemistry, western blot, and rt-pcr; tph activity was estimated by quantification of 5-hydroxytryptophan (5-htp) and, indirectly, through measurement of 5-ht and 5-hydroxindolacetic acid (5-hiaa) levels and turnover (5-hiaa/5-ht ratio) by hplc. 2024-10-28 2024-10-31 rat
Ruixiao Guo, Shengli Gao, Xufei Feng, Hua Liu, Xing Ming, Jinqiu Sun, Xinchi Luan, Zhenyu Liu, Weiyi Liu, Feifei Gu. The GABAergic pathway from anterior cingulate cortex to lateral hypothalamus area regulates irritable bowel syndrome in mice and its underlying mechanism. Journal of neurochemistry. 2024-06-15. PMID:38877776. enzyme-linked immunosorbent assay (elisa) and western blot analyses were conducted to determine the expression of histamine, 5-hydroxytryptamine (5-ht), and transient receptor potential vanilloid 4 (trpv4). 2024-06-15 2024-06-17 mouse
Haoyue Deng, Yi Wu, Po Gao, Dexu Kong, Chao Pan, Saihong Xu, Dan Tang, Yingfu Jiao, Daxiang Wen, Weifeng Y. Preoperative Pain Facilitates Postoperative Cognitive Dysfunction via PAG-DR Circuit. Neuroscience. 2023-03-23. PMID:36958595. the immunofluorescence staining and western blot results showed that tryptophan hydroxylase 2 (tph2) for serotonin synthesis in the dr was increased significantly in the rats treated with cfa for 7 days and sevoflurane for 3 hours, while chemo-genetic inhibition of the vlpag-dr projection induced obvious spatial learning and memory impairment. 2023-03-23 2023-08-14 rat
Lili Zou, Yaling Tian, Yuanfei Wang, Dongliang Chen, Xiaomin Lu, Ze Zeng, Zumin Chen, Chenli Lin, Yinji Lian. High-cholesterol diet promotes depression- and anxiety-like behaviors in mice by impact gut microbe and neuroinflammation. Journal of affective disorders. 2023-02-04. PMID:36738999. 5-ht (also named 5-hydroxytryptamine, 5-ht) and related receptors expression were monitored by elisa, rt-pcr and western blot. 2023-02-04 2023-08-14 mouse
Pan Meng, Xi Zhang, Dandan Li, Hui Yang, Xiaoyuan Lin, Hongqing Zhao, Ping Li, Yuhong Wang, Xiaoye Wang, Jinwen G. Leonurine Regulates Hippocampal Nerve Regeneration in Rats with Chronic and Unpredictable Mild Stress by Activating SHH/GLI Signaling Pathway and Restoring Gut Microbiota and Microbial Metabolic Homeostasis. Neural plasticity. vol 2023. 2023-01-17. PMID:36647544. western blot was utilized to measure the expression of bdnf and 5-ht in the hippocampus. 2023-01-17 2023-08-14 rat
Pei He, Binjun He, Shu Li, Wen Chai, Wei Rao, Yu Zhu, Wenzhi Chen, Ping Zhang, Xiong Zhang, Haili Pan, Renshi X. Distribution Features and Potential Effects of Serotonin in the Cerebrum of SOD1 G93A Transgenic Mice. eNeuro. 2022-10-20. PMID:36265904. therefore, we observed the distribution features and potential effects of 5-ht in the cerebrum of g93a sod1 transgenic (tg) and wild-type (wt) mice by fluorescence immunohistochemistry, western blot and enzyme-linked immunosorbent assays, as well as motor function measurements. 2022-10-20 2023-08-14 mouse
Ping Tian, Wei Zhang, Kai-Yan Li, Hong-Wei Li, Kai Ma, De-En Ha. [Effect of Rehmanniae Radix on depression-like behavior and hippocampal monoamine neurotransmitters of chronic unpredictable mild stress model rats]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 17. 2022-09-27. PMID:36164876. western blot was used to detect the protein expressions of tryptophan hydroxylase 2(tph2), serotonin transporter(sert), and monoamine oxidase a(mao-a) in the hippocampus of rats. 2022-09-27 2023-08-14 rat
Ping Su, Shuxin Yan, Jian Yang, Junchao Tong, James Samsom, Fan You, Yun Li, Qiuyue Chen, Anlong Jiang, Dongxu Zhai, Jiahao Chen, Zuoli Sun, Jingjing Zhou, Min Liu, Frank J S Lee, Zhi-Qing David Xu, Xin Wang, Neil Vasdev, Albert H C Wong, Fang Li. Serum amyloid P component (SAP) modulates antidepressant effects through promoting membrane insertion of the serotonin transporter. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology. 2022-09-08. PMID:36076020. we examined the effect of sap on serotonin transporter (sert) expression and localization using western blot, confocal microscopy, and positron emission tomography with the radioligand [ 2022-09-08 2023-08-14 human
Wenfei Wang, Yichun Wang, Qiuting Guo, Huiting Li, Zhaoqiang Wang, Jia Li, Taotao Li, Tiantian Tang, Yujiao Wang, Yanzhuo Jia, Yao Wang, Junbo Zou, Yajun Shi, Dongyan Guo, Ming Yang, Xiaofei Zhang, Jing Su. Valerian essential oil for treating insomnia Frontiers in nutrition. vol 9. 2022-08-22. PMID:35990355. valerian essential oil was given orally for treatment and the contents of 5-hydroxytryptamine receptor 1 a (5-ht1ar), γ-aminobutyric acid (gaba), cyclic adenosine monophosphate (camp), and protein kinase a (pka) in the hippocampus of rats in each group were detected by enzyme-linked immunosorbent assay (elisa), western blot, polymerase chain reaction (pcr), and immunohistochemistry. 2022-08-22 2023-08-14 rat
Kazi M Hoque, Mikio Hayashi, Irshad A Sheikh, Aditi Banerjee, Subhash C Verma, Normand Leblanc, Caroline J Zeiss, Nadia Ameen, Subhra Chakrabort. Elevated 5-hydroxytryptamine in COVID-19 Stimulates ANO1 Mediated Cl Secretion in Lung & Intestinal Epithelial Cells. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. vol 36 Suppl 1. 2022-05-13. PMID:35553771. using western blot, immunostaining, and electrophysiology, we characterized the localization of human ano1 and its stimulation by 5-ht on cl secretion in lung and intestinal epithelium. 2022-05-13 2023-08-13 human
Shanshan Liu, Qing Liu, Yanjie Ju, Lei Li. Downregulation of miR-383 reduces depression-like behavior through targeting Wnt family member 2 (Wnt2) in rats. Scientific reports. vol 11. issue 1. 2021-11-08. PMID:33927285. the expression levels of mirnas were detected by qrt-pcr, and the expression levels of wnt2, depression-impacted proteins (gfap, bdnf, creb), brain neurotransmitters (5-ht, ne, da) and apoptosis-related proteins (bax and bcl-2) were evaluated by qrt-pcr and western blot. 2021-11-08 2023-08-13 rat
Emiri Tarbit, Indu Singh, Jason Nigel Peart, Svetlana Bivol, Roselyn Barbara Rose'Meye. Increased release of serotonin from rat primary isolated adult cardiac myofibroblasts. Scientific reports. vol 11. issue 1. 2021-10-22. PMID:34645867. serotonin metabolism and receptor protein expression was assessed using western blot techniques and serotonin levels measured using elisa. 2021-10-22 2023-08-13 rat
Carolina Aparecida Faria Almeida, Antonio Alves Pereira-Junior, Jéssica Gonçalves Rangel, Bruna Pinheiro Pereira, Karla Cristinne Mancini Costa, Vitor Bruno, Gabriela Oliveira Silveira, Carla Speroni Ceron, Mauricio Yonamine, Rosana Camarini, Raphael Caio Tamborelli Garcia, Tania Marcourakis, Larissa Helena Torre. Ayahuasca, a psychedelic beverage, modulates neuroplasticity induced by ethanol in mice. Behavioural brain research. vol 416. 2021-10-05. PMID:34437939. dopamine receptors, serotonin receptors, dynorphin, and prodynorphin levels were quantified in the striatum and hippocampus by blot analysis. 2021-10-05 2023-08-13 mouse
Xin Ye, Bo-Chul Shin, Claire Baldauf, Amit Ganguly, Shubhamoy Ghosh, Sherin U Devaska. Developing Brain Glucose Transporters, Serotonin, Serotonin Transporter, and Oxytocin Receptor Expression in Response to Early-Life Hypocaloric and Hypercaloric Dietary, and Air Pollutant Exposures. Developmental neuroscience. vol 43. issue 1. 2021-08-25. PMID:33774619. employing maternal diet restriction (intrauterine growth restriction [iugr]), high-fat (hf) dietary modifications, and prenatal exposure to simulated ap, fetal (e19) murine brain 5-ht was assessed by elisa with sert and oxtr being localized by immunohistochemistry and measured by quantitative western blot analysis. 2021-08-25 2023-08-13 Not clear
He Yu, Zhong-Mei He, Kun Shi, Yan Zhao, Ying Zong, Jian-Ming Li, Wei-Jia Chen, Rui D. [Study on antidepressant effect of total alkaloids of Fibraurea recisa]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 46. issue 14. 2021-08-18. PMID:34402292. this study aimed to investigate the antidepressant effects of total alkaloids of fibraurea recisa in ht22 cells damaged by corticosterone (cort) in vitro and in a mouse model of chronic unpredictable mild stress (cums) as well as the underlying mechanisms.in cellular experiments,the viability of cort-damaged ht22 cells was detected using cell counting kit-8 (cck-8),and the cell apoptosis was detected by hoechst 33258 staining.in animal experiments,c57bl/6n mice were randomly divided into the control group,model group,low (100 mg·kg~(-1)),medium (200 mg·kg~(-1)) and high (400 mg·kg~(-1))-dose of total alkaloids of f.recisa groups,and positive control group.after 21 days of cums exposure,their depressive behaviors were observed in behavioral and morris water maze tests.the serum levels of 5-hydroxytryptamine (5-ht),dopamine (da),and norepinephrine (ne) were assessed by elisa.the expression levels of apoptosis-related proteins bcl-2,bax and cleaved caspase-3 in ht22 cells and mouse hippocampus were detected by western blot.the results suggested that total alkaloids of f.recisa alleviated the damage of ht22 cells induced by cort in a dose-dependent manner.the hoechst 33258 staining uncovered that total alkaloids of f.recisa better reduced the blue spots and inhibited cell apoptosis.the results of animal experiments showed that total alkaloids of f.recisa significantly improved the depression-like behaviors of mice and increased the serum levels of 5-ht,da and ne as compared with those in the model group.the western blot assays revealed a significant up-regulation of bcl-2 protein expression,but an obvious reduction in bax and cleaved caspase-3protein expression in the total alkaloids of f.recisa group.in conclusion,total alkaloids of f.recisa inhibited depression possibly by regulating the apoptosis-related protein expression or elevating the monoamine neurotransmitter levels in the brain. 2021-08-18 2023-08-13 mouse
Hui-Rong Guo, Bai-Ling Huang, Ya-Li Wang, Yan-Yan Zhang, Quan-Gang Ma, Pei-Pei Lv, Yu-Ming Re. Effect of Escitalopram on Serum GDNF and BDNF Levels and 5-HT Level of Brain Tissue of Obsessive-Compulsive Disorder Rats. Cellular and molecular neurobiology. vol 40. issue 6. 2021-01-22. PMID:31927718. in addition, western blot was used to determine the brain tissue protein levels of gdnf and bdnf and high-performance liquid chromatography + electrochemistry method to determine the 5-ht level of brain tissue. 2021-01-22 2023-08-13 rat
Yun-Yun Shao, Yi-Ting Guo, Jian-Ping Gao, Jun-Jin Liu, Zhuang-Peng Chang, Xiao-Juan Feng, Ding Xu, Gui-Feng Deng, Rui-Gang Ho. Shaoyao-Gancao Decoction Relieves Visceral Hyperalgesia in TNBS-Induced Postinflammatory Irritable Bowel Syndrome via Inactivating Transient Receptor Potential Vanilloid Type 1 and Reducing Serotonin Synthesis. Evidence-based complementary and alternative medicine : eCAM. vol 2020. 2020-10-31. PMID:33123210. rt-qpcr and western blot analyses were employed to detect the gene and protein level of tryptophan hydroxylase (tph), serotonin reuptake transporter (sert), and transient receptor potential vanilloid 1 (trpv1). 2020-10-31 2023-08-13 rat
Lin Ru Wang, Sang-Hoon Kim, Seung-Soo Bae. Effects of treadmill exercise on the anxiety-like behavior through modulation of GSK3β/β-catenin signaling in the maternal separation rat pup. Journal of exercise rehabilitation. vol 15. issue 2. 2020-10-01. PMID:31111002. for this study, elevated plus maze test, immunohistochemistry for serotonin (5-hydroxytryptamine, 5-ht), tryptophan hydroxylase (tph), and western blot for total gsk3β (t-gsk3β), phosphorylated gsk3β (p-gsk3β), total β-catenin (t-β-catenin), and phosphorylated β-catenin (p-β-catenin) were conducted. 2020-10-01 2023-08-13 rat
Mal-Soon Shin, Tae-Woon Kim, Jae-Min Lee, Yun-Hee Sung, Baek-Vin Li. Treadmill exercise alleviates depressive symptoms in rotenone-induced Parkinson disease rats. Journal of exercise rehabilitation. vol 13. issue 2. 2020-09-30. PMID:28503522. forced swimming test, immunohistochemistry for serotonin (5-hydroxytryptamine, 5-ht), tryptophan hydroxylase (tph), and western blot for serotonin 1a (5-ht1a) receptor were conducted. 2020-09-30 2023-08-13 rat
Hui Li, Tong Wang, Cuige Shi, Yutao Yang, Xiaoxiao Li, Yan Wu, Zhi-Qing David X. Inhibition of GALR1 in PFC Alleviates Depressive-Like Behaviors in Postpartum Depression Rat Model by Upregulating CREB-BNDF and 5-HT Levels. Frontiers in psychiatry. vol 9. 2020-09-30. PMID:30487761. moreover, western blot and hplc assays demonstrated that the downregulation of creb-bdnf signaling and 5-ht levels in the pfc of ppd rats were reversed after galr1-sirna injection. 2020-09-30 2023-08-13 rat