All Relations between blot and diaphragm

Publication Sentence Publish Date Extraction Date Species
Yurou Wang, Yun Chu, Hongkai Dai, Yingfang Zheng, Renyu Chen, Chenchen Zhou, Yanxia Zhong, Chengye Zhan, Jinlong Lu. Protective role of pretreatment with Anisodamine against sepsis-induced diaphragm atrophy via inhibiting JAK2/STAT3 pathway. International immunopharmacology. vol 133. 2024-04-23. PMID:38652962. the expression of murf1, atrogin1 and jak2/stat3 signaling pathway components in the diaphragm and c2c12 myotubes was measured by rt-qpcr and western blot. 2024-04-23 2024-04-26 mouse
Marcos Maciel Junior, Samara Camaçari de Carvalho, Paula Andrea Saenz Suarez, Humberto Santo Neto, Maria Julia Marque. Fish oil attenuated dystrophic muscle markers of inflammation via FFA1 and FFA4 in the mdx mouse model of DMD. Anatomical record (Hoboken, N.J. : 2007). vol 304. issue 6. 2021-09-30. PMID:33136305. after 1 month of treatment, plasma markers of myonecrosis (total and cardiac creatine kinase; ck), the levels of ffa1 and ffa4 and of the markers of inflammation, nuclear transcription factor kappa b (nfkb), tumor necrosis factor alpha (tnf-α) and interleukin 1β (il-1β) were analyzed in the diaphragm muscle and heart by western blot. 2021-09-30 2023-08-13 mouse
Age Kärssin, Noora Remes, Kaidi Korge, Mare Viigipuu, Christen Rune Stensvold, Maria Angeles Gómez-Morales, Alessandra Ludovisi, Pikka Jokelainen, Brian Lasse. HERBIVORES AS ACCIDENTAL HOSTS FOR TRICHINELLA: SEARCH FOR EVIDENCE OF TRICHINELLA INFECTION AND EXPOSURE IN FREE-RANGING MOOSE (ALCES ALCES) IN A HIGHLY ENDEMIC SETTING. Journal of wildlife diseases. vol 57. issue 1. 2021-06-22. PMID:33635991. a total of 460 moose were tested directly by artificial digestion of diaphragm muscle, 463 moose were tested indirectly by enzyme-linked immunosorbent assay (elisa), and 34 moose also by western blot. 2021-06-22 2023-08-13 Not clear
Joseph X DiMari. KLF10 Gene Expression Modulates Fibrosis in Dystrophic Skeletal Muscle. The American journal of pathology. vol 188. issue 5. 2019-04-22. PMID:29458012. on the basis of rt-pcr, western blot, and histological analyses of mouse tibialis anterior and diaphragm muscles, collagen type i (col1a1) and fibronectin gene expression and protein deposition were increased in klf10 2019-04-22 2023-08-13 mouse
Jin Wu, Tian Jin, Hong Wang, Shi-Tong L. Sepsis decreases the activity of acetylcholinesterase by reducing its expression at the neuromuscular junction. Molecular medicine reports. vol 16. issue 4. 2018-06-08. PMID:28849127. furthermore, ache expression levels at the nmj, and in the whole muscle fibers of the diaphragm, were detected by immunohistofluorescence staining and western blot analysis, respectively. 2018-06-08 2023-08-13 rat
Wanming Zhao, Xingyu Wang, Kai-Hui Sun, Lan Zho. α-smooth muscle actin is not a marker of fibrogenic cell activity in skeletal muscle fibrosis. PloS one. vol 13. issue 1. 2018-03-09. PMID:29320561. α-sma expression was detected by quantitative rt-pcr and western blot in fibrogenic cells sorted from diaphragm and quadriceps muscles of the coli-gfp/mdx5cv mice. 2018-03-09 2023-08-13 mouse
Guangyu Jiao, Liying Hao, Mengmeng Wang, Bin Zhong, Miao Yu, Shuang Zhao, Pingping Wang, Rui Feng, Shutao Tan, Liu Che. Upregulation of endoplasmic reticulum stress is associated with diaphragm contractile dysfunction in a rat model of sepsis. Molecular medicine reports. vol 15. issue 1. 2017-03-30. PMID:27959404. the mrna and protein expression of glucose‑regulated protein 78 kda (grp78), glucose-regulated protein 94 kda (grp94), c/ebp homologous protein (chop), endoplasmic reticulum protein 44 (erp44), protein disulfide-isomerase like protein (erp57) and protein disulfide isomerase family a member 4 (erp72) in diaphragm muscles were measured using reverse transcription‑quantitative polymerase chain reaction and western blot analysis. 2017-03-30 2023-08-13 rat
Jisheng Zhang, Haiyan Fu, Yan Xu, Yunfei Niu, Xiaofei A. Hyperoside reduces albuminuria in diabetic nephropathy at the early stage through ameliorating renal damage and podocyte injury. Journal of natural medicines. vol 70. issue 4. 2017-03-22. PMID:27255369. further investigations via immunofluorescence staining, real-time reverse transcription polymerase chain reaction and western blot analysis showed that the decreased slit diaphragm protein nephrin and podocin mrna expression and protein levels in dn mice were restored by hyperoside treatment. 2017-03-22 2023-08-13 mouse
Leticia Montanholi Apolinário, Samara Camaçari De Carvalho, Humberto Santo Neto, Maria Julia Marque. Long-Term Therapy With Omega-3 Ameliorates Myonecrosis and Benefits Skeletal Muscle Regeneration in Mdx Mice. Anatomical record (Hoboken, N.J. : 2007). vol 298. issue 9. 2016-05-11. PMID:26011009. the quadriceps and diaphragm muscles were removed and processed for histopathology and western blot. 2016-05-11 2023-08-13 mouse
Joanne M Bando, Mario Fournier, Xiaoyu Da, Michael I Lewi. Effects of malnutrition with or without eicosapentaenoic acid on proteolytic pathways in diaphragm. Respiratory physiology & neurobiology. vol 180. issue 1. 2012-04-04. PMID:22019487. pathways mediating muscle proteolysis with severe short-term nutritional deprivation (nd)±epa were evaluated, including diaphragm fiber-specific cross-sectional areas, mrna (real-time pcr) and protein expression (western blot). 2012-04-04 2023-08-12 rat
Cíntia Yuri Matsumura, Ana Paula Tiemi Taniguti, Adriana Pertille, Humberto Santo Neto, Maria Julia Marque. Stretch-activated calcium channel protein TRPC1 is correlated with the different degrees of the dystrophic phenotype in mdx mice. American journal of physiology. Cell physiology. vol 301. issue 6. 2012-01-18. PMID:21900691. western blot analysis showed higher levels of trpc1 in diaphragm muscle compared with stn and limb muscles. 2012-01-18 2023-08-12 mouse
M E Schuppers, G Rosenberg, R Graf, V Eidam, C Wittwer, W Zimmermann, B Gottstein, C F Fre. A study to demonstrate freedom from Trichinella spp. in domestic pigs in Switzerland. Zoonoses and public health. vol 57. issue 7-8. 2011-04-08. PMID:19968841. an elisa was used as the initial screening test, and sera reacting in elisa were further investigated using both a western blot for serology and an artificial digestion test with 20 g of diaphragm tissue for larval detection. 2011-04-08 2023-08-12 Not clear
Ping Huang, Xinyu S Zhao, Matthew Fields, Richard M Ransohoff, Lan Zho. Imatinib attenuates skeletal muscle dystrophy in mdx mice. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. vol 23. issue 8. 2009-08-18. PMID:19289603. phosphorylation of the tyrosine kinase targets of imatinib was assessed by western blot in diaphragm tissue and in primary cultures of peritoneal macrophages and skeletal muscle fibroblasts. 2009-08-18 2023-08-12 mouse
Helio C Manso Filho, Kenneth H McKeever, Mary E Gordon, Helena Emilia C Costa, Malcolm Watfor. Novel findings regarding Glut-4 expression in adipose tissue and muscle in horses--a preliminary report. Veterinary journal (London, England : 1997). vol 174. issue 3. 2008-02-27. PMID:17174126. glut-4 expression was determined by western blot using samples obtained from post mortem biopsies obtained from four muscles (gluteus medius, semitendinosus, heart, and diaphragm), and four adipose tissues (subcutaneous, retroperitoneal, mesenteric, and omental) in three horses. 2008-02-27 2023-08-12 Not clear
Stefan Matecki, François Rivier, Gerald Hugon, Christelle Koechlin, Alain Michel, Christian Prefaut, Dominique Mornet, Michele Ramonatx. The effect of respiratory muscle training with CO2 breathing on cellular adaptation of mdx mouse diaphragm. Neuromuscular disorders : NMD. vol 15. issue 6. 2005-07-25. PMID:15907290. western blot analysis of diaphragm showed: (1) an over-expression of alpha-dystrobrevin in mdx and c57bl10 training group compared to control group (8100+/-710 versus 6100+/-520 and 2800+/-400 versus 2200+/-250 arbitrary units); (2) a decrease in utrophin expression only in mdx training group compared to control group (2100+/-320 versus 3100+/-125 arbitrary units). 2005-07-25 2023-08-12 mouse
Natalia Gousseva, Rohan T Bake. Gene structure, alternate splicing, tissue distribution, cellular localization, and developmental expression pattern of mouse deubiquitinating enzyme isoforms Usp2-45 and Usp2-69. Gene expression. vol 11. issue 3-4. 2004-02-02. PMID:14686789. using northern blot, western blot, and immunohistochemistry, usp2 expression was observed in many adult and embryonic tissues including testis, heart, skeletal muscle, diaphragm, brain, kidney, liver, pancreas, lung, and skin. 2004-02-02 2023-08-12 mouse
M de Paula Brotto, S A van Leyen, L S Brotto, J P Jin, C M Nosek, T M Nose. Hypoxia/fatigue-induced degradation of troponin I and troponin C: new insights into physiologic muscle fatigue. Pflugers Archiv : European journal of physiology. vol 442. issue 5. 2001-12-11. PMID:11512030. we report for the first time that the effects of hypoxia-fatigue, namely to decrease maximal tetanic force, maximal calcium-activated force and calcium sensitivity of the mouse diaphragm muscle, are associated with the degradation of troponins tni and tnc (western blot analysis). 2001-12-11 2023-08-12 mouse
J Gea, Q Hamid, G Czaika, E Zhu, V Mohan-Ram, G Goldspink, A Grassin. Expression of myosin heavy-chain isoforms in the respiratory muscles following inspiratory resistive breathing. American journal of respiratory and critical care medicine. vol 161. issue 4 Pt 1. 2000-06-08. PMID:10764323. mrna expression was assessed in the diaphragm, external intercostal muscle, and a limb muscle, using both slot- blot and in situ hybridizations with isoform-specific probes. 2000-06-08 2023-08-12 Not clear
M Balage, D Larbaud, E Debras, J F Hocquette, J Grizar. Acute hyperinsulinemia fails to change GLUT-4 content in crude membranes from goat skeletal muscles and adipose tissue. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. vol 120. issue 3. 1998-12-15. PMID:9787827. glut-4 protein was assessed in crude membrane preparations from adipose tissue and four skeletal muscles (longissimus dorsi, tensor fasciae latae, anconeus and diaphragm) by western blot analysis. 1998-12-15 2023-08-12 Not clear
G Fiermonte, V Dolce, F Palmier. Expression in Escherichia coli, functional characterization, and tissue distribution of isoforms A and B of the phosphate carrier from bovine mitochondria. The Journal of biological chemistry. vol 273. issue 35. 1998-09-24. PMID:9712911. western blot analysis demonstrated that isoform a is present in high amounts in heart, skeletal muscle, and diaphragm mitochondria, whereas isoform b is present in the mitochondria of all tissues examined. 1998-09-24 2023-08-12 cattle