| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| W Lee-Kwon, D Park, M Bernie. Nucleotide excision repair is not required for the antiapoptotic function of insulin-like growth factor 1. Experimental cell research. vol 241. issue 2. 1998-07-14. PMID:9637787. |
igf-1 effectively blocked apoptosis, concomitant with increased mtt activity, in a pair of cho cell lines expressing inactive ercc1 (43-3b cells) and the transfected line of the mutant carrying the expressed human ercc1 gene (83-g5 cells). |
1998-07-14 |
2023-08-12 |
human |
| H Burger, K Nooter, A W Boersma, C J Kortland, G Stote. Expression of p53, Bcl-2 and Bax in cisplatin-induced apoptosis in testicular germ cell tumour cell lines. British journal of cancer. vol 77. issue 10. 1998-06-29. PMID:9635829. |
for all four cell lines, the induction of apoptosis by cisplatin correlated with drug sensitivity in the mtt assay. |
1998-06-29 |
2023-08-12 |
Not clear |
| Y Zhao, J Cao, H Ma, J Li. Apoptosis induced by tea polyphenols in HL-60 cells. Cancer letters. vol 121. issue 2. 1998-05-13. PMID:9570354. |
the apoptosis effects of tea polyphenols were observed on the human promyelocytic leukemic cells (hl-60) by an mtt reduction test, dna agarose gel electrophoresis and a transmission electronic microscopy technique. |
1998-05-13 |
2023-08-12 |
human |
| K Nonaka, A Ishisaki, M Muro, S Kato, M Oido, K Nakashima, Y Kowashi, T Nishihar. Possible involvement of protein kinase C in apoptotic cell death of macrophages infected with Actinobacillus actinomycetemcomitans. FEMS microbiology letters. vol 159. issue 2. 1998-03-26. PMID:9503618. |
in this study, we examined the role of protein kinases in the induction of apoptosis in a. actinomycetemcomitans-infected j774.1 cells by the mtt assay, fluorescence microscopy and flow cytometric analysis. |
1998-03-26 |
2023-08-12 |
mouse |
| M Matsuoka, B Wispriyono, H Igis. Induction of c-fos gene by mercury chloride in LLC-PK1 cells. Chemico-biological interactions. vol 108. issue 1-2. 1998-02-24. PMID:9463523. |
the cytotoxicity of hgcl2 as assayed by mitochondrial dehydrogenase activity (mtt conversion) was observed after 18 h exposure but not at 0.5-8 h. also, the decrease in cell viability was accompanied with dna fragmentation, which is characteristic of apoptosis. |
1998-02-24 |
2023-08-12 |
Not clear |
| T Efferth, U Fabry, R Osiek. Apoptosis and resistance to daunorubicin in human leukemic cells. Leukemia. vol 11. issue 7. 1997-07-17. PMID:9205009. |
on the other hand, we used the mtt assay and measured apoptosis and proliferative activity (s- and g2m-phases of the cell cycle) by flow cytometry. |
1997-07-17 |
2023-08-12 |
human |
| T Efferth, U Fabry, R Osiek. Apoptosis and resistance to daunorubicin in human leukemic cells. Leukemia. vol 11. issue 7. 1997-07-17. PMID:9205009. |
low proficiency of mnc to undergo apoptosis and low proliferative activity rather than p-gp-mediated mdr correlated with dnr resistance as measured by the mtt assay. |
1997-07-17 |
2023-08-12 |
human |
| T Efferth, U Fabry, R Osiek. Apoptosis and resistance to daunorubicin in human leukemic cells. Leukemia. vol 11. issue 7. 1997-07-17. PMID:9205009. |
thus, measurement of apoptosis by flow cytometry may be less convenient than the mtt assay for determination of chemosensitivity, if clinical samples with unknown patterns of responsiveness are to be tested. |
1997-07-17 |
2023-08-12 |
human |
| T Efferth, U Fabry, R Osiek. Apoptosis and resistance to daunorubicin in human leukemic cells. Leukemia. vol 11. issue 7. 1997-07-17. PMID:9205009. |
spontaneous apoptosis in untreated mnc following 24 h incubation in vitro correlated significantly with dnr sensitivity in the mtt assay. |
1997-07-17 |
2023-08-12 |
human |
| Y Huang, A M Ibrado, J C Reed, G Bullock, S Ray, C Tang, K Bhall. Co-expression of several molecular mechanisms of multidrug resistance and their significance for paclitaxel cytotoxicity in human AML HL-60 cells. Leukemia. vol 11. issue 2. 1997-02-24. PMID:9009089. |
pgp, but not mrp, overexpression significantly impaired paclitaxel accumulation and paclitaxel-induced apoptosis, as well as reduced its cytotoxic effects as determined by the mtt assay. |
1997-02-24 |
2023-08-12 |
human |
| M Párrizas, A R Saltiel, D LeRoit. Insulin-like growth factor 1 inhibits apoptosis using the phosphatidylinositol 3'-kinase and mitogen-activated protein kinase pathways. The Journal of biological chemistry. vol 272. issue 1. 1997-02-18. PMID:8995241. |
after 4-6 h of serum deprivation, apoptosis was initiated, concomitant with a 30% decrease in cell number and a 75% decrease in mtt activity. |
1997-02-18 |
2023-08-12 |
Not clear |
| G Basha, P Yap, F Penninck. Comparative study of classical, colorimetric and immunologic staining methods for the assessment of tumor cell viability. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. vol 17. issue 6. 1997-01-16. PMID:8938951. |
the trypan blue exclusion test, the mtt test and an immunostaining test for apoptosis were performed before and after incubation of sw620 human colonic carcinoma cells with different cytotoxic agents (ctas) in order to assess tumor cell viability and cta efficacy in vitro. |
1997-01-16 |
2023-08-12 |
human |
| G Bullock, S Ray, J C Reed, S Krajewski, A M Ibrado, Y Huang, K Bhall. Intracellular metabolism of Ara-C and resulting DNA fragmentation and apoptosis of human AML HL-60 cells possessing disparate levels of Bcl-2 protein. Leukemia. vol 10. issue 11. 1996-12-03. PMID:8892676. |
significantly greater loss of survival (by mtt assay) and flowcytometric and morphologically recognizable apoptosis were observed in hl-60/neo cells. |
1996-12-03 |
2023-08-12 |
human |
| B Björkerud, S Björkeru. Contrary effects of lightly and strongly oxidized LDL with potent promotion of growth versus apoptosis on arterial smooth muscle cells, macrophages, and fibroblasts. Arteriosclerosis, thrombosis, and vascular biology. vol 16. issue 3. 1996-07-02. PMID:8630668. |
growth was evaluated by measuring the synthesis of dna and culture size (mtt method) and apoptosis by using the in situ labeling of internucleosomally degraded dna and, in the case of smcs, the appearance of chromatin condensation. |
1996-07-02 |
2023-08-12 |
human |
| C M Cagnoli, C Atabay, E Kharlamova, H Mane. Melatonin protects neurons from singlet oxygen-induced apoptosis. Journal of pineal research. vol 18. issue 4. 1996-01-30. PMID:8531053. |
we found that this procedure triggers neuronal death, which is preceded by mitochondrial impairment (assayed by the rate of the reduction of mtt, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide, into formazan), and by dna fragmentation--a marker of apoptosis. |
1996-01-30 |
2023-08-12 |
Not clear |
| Y Ueno, K Umemori, E Niimi, S Tanuma, S Nagata, M Sugamata, T Ihara, M Sekijima, K Kawai, I Uen. Induction of apoptosis by T-2 toxin and other natural toxins in HL-60 human promyelotic leukemia cells. Natural toxins. vol 3. issue 3. 1995-09-28. PMID:7648021. |
detailed experiments on dose and time dependencies revealed that t-2 toxin induced the apoptosis at 10 ng/ml (= 4 x 10(-8) m) levels within 2-6 hr without significant cytotoxicity evaluated by the dye exclusion and mtt. |
1995-09-28 |
2023-08-12 |
human |
| R Graber, G A Los. Changes in the activities of signal transduction and transport membrane enzymes in CEM lymphoblastoid cells by glucocorticoid-induced apoptosis. Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology. vol 8. issue 2. 1995-07-27. PMID:7786813. |
apoptosis was seen after incubation with 10(-6)m dexamethasone for 48 h, with decreased cell number, cellular activation (mtt) and s-phase percentage; enhanced dna fragmentation and propidium iodide uptake; and ultrastructural changes in the chromatin and cell membranes. |
1995-07-27 |
2023-08-12 |
Not clear |
| A M del Llano, C Zorrilla, J A Lavergn. Multifunctional immunological monitoring of HIV positive patients: a novel staging system. Puerto Rico health sciences journal. vol 12. issue 4. 1994-04-22. PMID:8140207. |
the classifying functional parameters are: the mitochondrial metabolic activity of freshly isolated bmnc, measured by an mtt reduction assay, the detection of apoptosis in 72 hour cultures of these cells assessed by propidium iodide staining and dual parametric flow cytometric analysis, and their proliferative response to pokeweed mitogen. |
1994-04-22 |
2023-08-12 |
Not clear |
| A A van de Loosdrecht, G J Ossenkoppele, R H Beelen, M G Broekhoven, A M Dräger, M M Langenhuijse. Apoptosis in tumor necrosis factor-alpha-dependent, monocyte-mediated leukemic cell death: a functional, morphologic, and flow-cytometric analysis. Experimental hematology. vol 21. issue 13. 1993-12-23. PMID:8243565. |
tnf-alpha-induced apoptosis (10(4) u/ml) as measured by multiparameter flow cytometry (propidium iodide [pi]) paralleled the functional decrease in cell viability (mtt assay) of 20 +/- 3% after 24 hours up to a maximum of 50 +/- 4% after 48 hours. |
1993-12-23 |
2023-08-12 |
human |
| A A van de Loosdrecht, G J Ossenkoppele, R H Beelen, M G Broekhoven, A M Dräger, M M Langenhuijse. Apoptosis in tumor necrosis factor-alpha-dependent, monocyte-mediated leukemic cell death: a functional, morphologic, and flow-cytometric analysis. Experimental hematology. vol 21. issue 13. 1993-12-23. PMID:8243565. |
after prolonged incubation times, monocyte-mediated leukemic cell death could be quantified as apoptosis by flow cytometry, whereas no decrease in net cell viability of tumor cells relative to the initial cell number could be observed by mtt spectrophotometry. |
1993-12-23 |
2023-08-12 |
human |