| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| W H Kim, H W Schnaper, M Nomizu, Y Yamada, H K Kleinma. Apoptosis in human fibrosarcoma cells is induced by a multimeric synthetic Tyr-Ile-Gly-Ser-Arg (YIGSR)-containing polypeptide from laminin. Cancer research. vol 54. issue 18. 1994-09-27. PMID:8069868. |
tumor cells treated with this peptide showed the expected morphological changes associated with apoptosis, acridine orange staining of nuclei, increased numbers of 3'-oh ends of dna in nuclei, a dna ladder pattern on agarose gels, and increased transforming growth factor beta 1 mrna by northern blot. |
1994-09-27 |
2023-08-12 |
human |
| W D Jarvis, A J Turner, L F Povirk, R S Traylor, S Gran. Induction of apoptotic DNA fragmentation and cell death in HL-60 human promyelocytic leukemia cells by pharmacological inhibitors of protein kinase C. Cancer research. vol 54. issue 7. 1994-04-28. PMID:7511048. |
finally, northern blot analysis revealed that exposure to calphostin c at a concentration that induced apoptosis (100 nm) failed to alter expression of bcl-2, an oncogene known to block apoptosis in both lymphoid and myeloid leukemia cells. |
1994-04-28 |
2023-08-12 |
human |
| A O Hueber, G Raposo, M Pierres, H T H. Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism. The Journal of experimental medicine. vol 179. issue 3. 1994-03-30. PMID:7906706. |
use of a quantitative dna dot blot assay indicated that thy-1-mediated thymocyte apoptosis was not blocked by rna or protein synthesis inhibitors, egta, or by cyclosporin a, and differed, therefore, from "activation-driven cell death". |
1994-03-30 |
2023-08-12 |
mouse |
| J Delic, M Morange, H Magdelena. Ubiquitin pathway involvement in human lymphocyte gamma-irradiation-induced apoptosis. Molecular and cellular biology. vol 13. issue 8. 1993-08-24. PMID:8393139. |
using in vitro nuclear transcription assays (run-on), northern (rna) blot analysis, immunolocalization studies, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis after immunoprecipitation, we demonstrate that (i) the ubiquitin mrna level is increased as a consequence of the activation of ubiquitin gene transcription 15 to 90 min after initiation of apoptosis; (ii) specifically in apoptotic cells, and not in all irradiated cells, nuclear proteins are highly ubiquitinated; and (iii) ubiquitin sequence-specific antisense oligonucleotide inhibition results in a decreased level of ubiquitinated nuclear proteins and considerably diminishes the proportion of cells exhibiting the apoptotic death pattern. |
1993-08-24 |
2023-08-12 |
human |
| A Facchinetti, L Tessarollo, M Mazzocchi, R Kingston, D Collavo, G Bias. An improved method for the detection of DNA fragmentation. Journal of immunological methods. vol 136. issue 1. 1991-03-22. PMID:1704901. |
an application of the southern blot technique is described which permits the detection of dna fragmentation due to cell death by apoptosis. |
1991-03-22 |
2023-08-11 |
Not clear |