| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Yanfeng Liu, Ming Zhan. Synergistic Anticancer Effects of Silibinin and Sulforaphane: Targeting Gastric Cancer via PI3K/AKT and ERK1/2 MAPK Pathway Inhibition and Molecular Docking Insights. Journal of biochemical and molecular toxicology. vol 39. issue 4. 2025-03-28. PMID:40152010. |
flow cytometry and western blot analysis were used to evaluate the protein profiles of cell survival, cell cycle, proliferation, and apoptosis. |
2025-03-28 |
2025-03-30 |
Not clear |
| Tong Li, Xiao-Hui Yang, Ming-Ju Shao, Yu-Xia Dong, Lin-Yu Li, Cun-Zhi Li. Effectiveness and mechanism of cisplatin combined with PDT on human lung adenocarcinoma A549 cells transplanted tumor in nude mice. Scientific reports. vol 15. issue 1. 2025-03-25. PMID:40128581. |
the apoptosis of tumor cells in the four groups was observed and compared by the tunel method, and the mrna expression levels of apoptosis-related genes bax, caspase-3 and survivin, as well as the expression levels of the corresponding proteins, were detected by the real-time fluorescence quantitative polymerase chain reaction (rt-qpcr) and the protein immunoblotting technique (western blot) respectively. |
2025-03-25 |
2025-03-27 |
mouse |
| Yunfei Ge, Yu Shi, Chunhong Wei, Dezhi Liu, Longkui Cao, Subramanian Palanisamy, Chongye Fan. Modification of soluble dietary fiber from quinoa bran and investigation of its anticancer activity in vitro. Journal of the science of food and agriculture. 2025-03-24. PMID:40125799. |
then, its ability to induce apoptosis in hepg2 human hepatoma cells was investigated using cell proliferation, scratch wound healing assay, flow cytometry, real-time polymerase chain reaction and western blot. |
2025-03-24 |
2025-03-26 |
human |
| Wenqian Zheng, Yang Liu, Jinru Liu, Yuping Zhao, Peiyu Wang, Yi Wang, Yuxuan Wang, Zeyu Lu, Xiaofan Liu, Yaru Shi, Na Zhou, Fermín Eduardo González, Hongchen Sun, Ding Zhou, Xiaowei X. Copper/calcium co-doped carbon dots for targeted cancer therapy with dual-mode imaging and synergistic induction of cuproptosis and calcium-mediated apoptosis. Journal of colloid and interface science. vol 690. 2025-03-21. PMID:40117884. |
mechanistic studies revealed that cu/ca-cds induced scc7 tumor cell death through copper-induced cuproptosis and calcium overload-mediated apoptosis, as confirmed by western blot, immunofluorescence staining, and rhod-2 calcium probe analyses. |
2025-03-21 |
2025-03-24 |
mouse |
| Zhaorong Yue, Fei Xie, Ruyue Wang, Xin Wang, Hongyu L. Lienal Polypeptide Decreases Immune Thrombocytopenia in a Mouse Model by Upregulating Cytokine Production and Increasing the Levels of CD4 Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research. 2025-03-20. PMID:40113259. |
we first assessed the effects of lp on meg-01 megakaryocytic cells regarding proliferation, apoptosis, and differentiation using methylthiazolyldiphenyl-tetrazolium bromide (mtt) assays, western blot analysis, and flow cytometry for apoptosis and cd41 expression as a differentiation marker. |
2025-03-20 |
2025-03-23 |
mouse |
| Yifei Wu, Xiaochuan Wang, Jing Liu, Zhiqiong Wang, Jinrong Li, Wenhe Zhang, Jie Xiang, Renfu Li, Sha Du, Chunping A. 308-nm excimer laser combined with piperine promotes melanin synthesis through PTEN/PDK1/GSK3b regulation of keratinocyte paracrine. Archives of dermatological research. vol 317. issue 1. 2025-03-19. PMID:40105984. |
elisa, western blot analysis, immunofluorescence and rt‒qpcr were used to measure the levels of proteins and genes, and edu, transwell, and flow cytometric assays were used to assess cell proliferation and apoptosis. |
2025-03-19 |
2025-03-22 |
Not clear |
| Simin Wu, Qixing Zhou, Ziqi Gao, Jiaxu Man, Wei He, Jingying Feng, Xiaoyong Li, Dongying Zhan. Chlorogenic acid targets SIRT6 to relieve UVB - induced UV damage. Archives of dermatological research. vol 317. issue 1. 2025-03-19. PMID:40105999. |
furthermore, the results of comet electrophoresis and western blot (wb) experiments demonstrate that cga and oss-128,167 (sirt6 inhibitor) can effectively inhibit dna damage caused by uvb, thereby alleviating cell apoptosis. |
2025-03-19 |
2025-03-22 |
Not clear |
| Peng Cheng, Huan Li, Hai-Wei Chen, Zhi-Qiang Wang, Pei-Wu Li, Hai-Hong Zhan. 5-Azacytidine inhibits endoplasmic reticulum stress and apoptosis of nucleus pulposus cells by preserving PPARγ via promoter demethylation. In vitro cellular & developmental biology. Animal. 2025-03-19. PMID:40102314. |
western blot (bcl-2, bax, caspase-3, chop, grp78, atf-6), tunel, and chop immunofluorescence results showed that 5aza attenuated il-1β-induced apoptosis and ers of np cells. |
2025-03-19 |
2025-03-21 |
Not clear |
| Jingya Chen, Zhenglei Ji, Di Wu, Siyang Wei, Wanjing Zhu, Guisen Peng, Mingjie Hu, Yunli Zhao, Huazhang W. MYBL2 promotes cell proliferation and inhibits cell apoptosis via PI3K/AKT and BCL2/BAX/Cleaved-caspase-3 signaling pathway in gastric cancer cells. Scientific reports. vol 15. issue 1. 2025-03-18. PMID:40097530. |
gc cell proliferation, cell cycle and apoptosis were determined by cck-8 and flow cytometry assays, and proteins were examined by western blot analysis. |
2025-03-18 |
2025-03-20 |
Not clear |
| Shubham Upadhayay, Vivek Uttam, Puneet Kuma. G-Protein-Coupled Estrogen Receptor 1 (GPER1) Activation Mitigates Haloperidol-Induced Neurotoxicity in SHSY-5Y Cells and Improves Motor Functions in Adult Zebrafish. Neurochemical research. vol 50. issue 2. 2025-03-15. PMID:40087195. |
moreover, cell viability was analyzed using mtt assay; apoptosis was done using a confocal microscope, and molecular mechanism investigated through western blot and qrt-pcr analysis. |
2025-03-15 |
2025-03-18 |
zebrafish |
| William C Cho, Chi F Won. Potential benefits of combined treatment with Hsp90 inhibitor AUY922 and cisplatin for overcoming drug resistance in nasopharyngeal carcinoma. American journal of cancer research. vol 15. issue 2. 2025-03-14. PMID:40084353. |
the response of cisplatin-resistant npc cells to auy922 and/or cisplatin was assessed through proliferation assay, cell cycle analysis, annexin v apoptosis detection, western blot analysis, |
2025-03-14 |
2025-03-16 |
human |
| Yi Liu, Yina Wang, Adili Salai, Yueying Yang, Yang Wan. Induction of miR-224 by Reactive Oxygen Species Regulates RASSF6 and Thus Modulates Malignant Behaviors and Chemosensitivity in Esophageal Squamous Cell Carcinoma. Journal of biochemical and molecular toxicology. vol 39. issue 3. 2025-03-11. PMID:40067291. |
the expression of mir-224 and hif-1α in esophageal cancer cells under hypoxic induction and their relationship with ros was studied using rt-qpcr and western blot assays; cell viability and apoptosis under hypoxia, as well as the effects of mir-224 on cell proliferation and drug resistance, were investigated using cck8, annexin v-fitc/pi, h2dcfda staining and western blot assays. |
2025-03-11 |
2025-03-14 |
Not clear |
| Tian-Wen Qiu, Zhan Jin, Zhi-Zhan Fu, Xin-Jiang Yan, Cheng-Peng Zhan, Hui-Wen Zheng, Mei-Ying Li, Guo-Feng Y. High-temperature requirement serine protease A2 inhibitor UCF-101 ameliorates damaged neurons in traumatic brain-injured rats by the AMPK/NF-κB pathway. Open life sciences. vol 20. issue 1. 2025-03-10. PMID:40059880. |
modified neurological severity score (mnss) cognitive function, cerebral edema index, hematoxylin-eosin staining, tunel staining for apoptosis, ethidium bromide staining for blood-brain barrier (bbb) permeability, enzyme-linked immunosorbent assay for inflammation response, and western blot analysis were performed. |
2025-03-10 |
2025-03-12 |
rat |
| Wenwen Yu, Yubo Shi, Xiaoqiong Bao, Xiangxiang Chen, Yangyang Ni, Jincong Wang, Hua Y. Hyaluronan-mediated motility receptor-mediated aerobic glycolysis enhances stem-like properties and chemoresistance in lung adenocarcinoma. The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology. 2025-03-07. PMID:40051130. |
stemness was evaluated through cell aggregation assays and western blot, and drug responsiveness was gauged using cck-8 assays, alongside flow cytometry for apoptosis analysis. |
2025-03-07 |
2025-03-09 |
Not clear |
| Ahmed M El-Dessouki, Mohamed E Kaml, Mohammed F El-Yaman. Modulation of AMPK by esomeprazole and canagliflozin mitigates methotrexate-induced hepatotoxicity: involvement of MAPK/JNK/ERK, JAK1/STAT3, and PI3K/Akt signaling pathways. Naunyn-Schmiedeberg's archives of pharmacology. 2025-03-07. PMID:40055205. |
liver injury, oxidative stress, inflammation, and apoptosis were assessed using biochemical, histopathological, immunohistochemical, qrt-pcr, and western blot analyses. |
2025-03-07 |
2025-03-12 |
rat |
| Wenya Du, Yumei Dai, Linzhi Yue, Tao Ma, Lixian W. [miR-207 targets autophagy-associated protein LAMP2 to regulate the mechanism of macrophage-mycobacterium tuberculosis interaction]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 41. issue 2. 2025-03-06. PMID:40047468. |
western blot analysis was performed to measure the relative expression of lamp2 proteins, apoptosis proteins, pyroptosis proteins, and autophagy proteins. |
2025-03-06 |
2025-03-08 |
Not clear |
| Wenya Du, Yumei Dai, Linzhi Yue, Tao Ma, Lixian W. [miR-207 targets autophagy-associated protein LAMP2 to regulate the mechanism of macrophage-mycobacterium tuberculosis interaction]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 41. issue 2. 2025-03-06. PMID:40047468. |
real-time fluorescence quantitative pcr and western blot analysis revealed that the relative expression of apoptosis and pyroptosis-related proteins and genes in the control group was lower in the op-lamp2 group compared to the ep group, and higher in the silamp2 group compared to the silamp2-nc group. |
2025-03-06 |
2025-03-08 |
Not clear |
| Jiluan Zhang, Zihan Chen, Shibo Wang, Lingxue Kong, Jinjuan Li. Prodelphinidin from purple sweet potato induces apoptosis in human triple-negative breast cancer cells via ROS-mediated ER stress activation. Medical oncology (Northwood, London, England). vol 42. issue 4. 2025-03-06. PMID:40048036. |
flow cytometry and western blot analysis revealed that pd induced cell apoptosis by down-regulating bcl-2, activating caspase-3/9, and cleaving parp. |
2025-03-06 |
2025-03-08 |
human |
| Fei Zhao, Guiqing Liu, Lijuan Xiong, Liang Yao, Lijun Wang, Zheng Zhan. Effect of sufentanil on the proliferation, apoptosis, and epithelial-mesenchymal transition of ovarian cancer cells by regulating the SMAD3/SNAIL signaling pathway. Journal of molecular histology. vol 56. issue 2. 2025-03-03. PMID:40032693. |
after 48 h of culture, cell migration was evaluated by the scratch assay; cell invasion was assessed using the transwell chamber assay; cell apoptosis was measured via flow cytometry; the growth status of the cells was observed under an optical microscope; the expression of n-cadherin, e-cadherin, smad3, tgf-β, and snail proteins was detected by western blot. |
2025-03-03 |
2025-03-06 |
Not clear |
| Jui-Ho Wang, Yun-Wen Chen, Shuchen Hsieh, Mei-Lang Kun. Nanosized Ginger-Derived Phenolic Zingerone Obstructs Cell Cycle G2/M Progression and Initiates Apoptosis in Human Colorectal Cancer. Environmental toxicology. 2025-02-26. PMID:40001302. |
western blot analysis also suggested that zingerone nps mediate cell apoptosis by upregulating caspase 3/parp signaling. |
2025-02-26 |
2025-02-28 |
human |