All Relations between apoptosis and blot

Publication Sentence Publish Date Extraction Date Species
Daxia Cai, Feng Tian, Minhua Wu, Jianfei Tu, Yonghui Wan. UBE2C is a diagnosis and therapeutic biomarker involved in immune infiltration of cancers including lung adenocarcinoma. Journal of Cancer. vol 15. issue 6. 2024-02-19. PMID:38370368. rna interference technology, cck8 assay, flow cytometry and apoptosis detection, and western blot were used for in vitro experiments. 2024-02-19 2024-02-21 Not clear
Enzhuang Pan, Yue Xin, Xueqing Li, Kaixin Ping, Xing Li, Ying Sun, Xuhui Xu, Jingquan Don. Immunoprotective effect of silybin through blocking p53-driven caspase-9-Apaf-1-Cyt c complex formation and immune dysfunction after difenoconazole exposure in carp spleen. Environmental science and pollution research international. 2024-02-15. PMID:38358624. by hematoxylin-eosin staining, dihydroethidium staining, immunohistochemical staining, terminal deoxynucleotidyl transferase-mediated dutp nick-end labeling assay, quantitative real-time pcr assay, western blot analysis, biochemical assays, and immune function indicator assays, we found that silybin could prevent difenoconazole-induced spleen tissue damage, oxidative stress, and immune dysfunction, and inhibited apoptosis of carp spleen tissue cells by suppressing the formation of p53-driven caspase-9-apoptotic protease activating factor-1-cytochrome c complex. 2024-02-15 2024-02-17 Not clear
Huawei Xu, Peng Shen, Jian Fang, Jihua Jiang, Yinsheng Shi, Pengcheng Xu, Renya Jiang, Zhengfei Wan. LINC00624 affects hepatocellular carcinoma proliferation and apoptosis through the miR-342-3p/DNAJC5 axis. Journal of biochemical and molecular toxicology. vol 38. issue 2. 2024-02-13. PMID:38348704. colony formation, flow cytometry and western blot tests were used to determine cell proliferation and apoptosis, respectively. 2024-02-13 2024-02-15 Not clear
Yin-Yin Zhao, Jun Li, Hao-Qi Wang, Hao-Bo Zheng, Shi-Wei Ma, Guang-Zhou Zho. Activation of autophagy promotes the inhibitory effect of curcumin analog EF-24 against MDA-MB-231 cancer cells. Journal of biochemical and molecular toxicology. vol 38. issue 2. 2024-02-13. PMID:38348710. ef-24 also induced mitochondrial apoptosis in mda-mb-231 cells as detected by hoechst 33342 staining, flow cytometry analysis, and western blot analysis. 2024-02-13 2024-02-15 Not clear
Nazım Abdülkadir Kankılıç, Hasan Şimşek, Nurhan Akaras, Cihan Gür, Mustafa İleritürk, Sefa Küçükler, Serkan A Akarsu, Fatih M Kandemi. Protective effects of naringin on colistin-induced damage in rat testicular tissue: Modulating the levels of Nrf-2/HO-1, AKT-2/FOXO1A, Bax/Bcl2/Caspase-3, and Beclin-1/LC3A/LC3B signaling pathways. Journal of biochemical and molecular toxicology. vol 38. issue 2. 2024-02-13. PMID:38348713. the parameters of oxidative stress, inflammation, apoptosis, and autophagic damage were evaluated by using biochemical, molecular, western blot, and histological methods in testicular issues. 2024-02-13 2024-02-15 rat
Ying Gao, Weijie Tao, Shoujun Wang, Ran Duan, Zhendong Zhan. AKR1C3 silencing inhibits autophagy-dependent glycolysis in thyroid cancer cells by inactivating ERK signaling. Drug development research. vol 85. issue 1. 2024-02-13. PMID:38349266. additionally, the expression of proteins related to cell cycle, apoptosis, glycolysis, autophagy, and extracellular signal-regulated kinase (erk) signaling in cells and tumor tissues was assessed by western blot. 2024-02-13 2024-02-15 mouse
Qiao-Hui Liu, Ke Zhang, Shu-Shu Feng, Li-Juan Zhang, Shun-Ying Li, Hang-Yu Wang, Jin-Hui Wan. Rosavin Alleviates LPS-Induced Acute Lung Injure by Modulating the TLR-4/NF-κB/MAPK Singnaling Pathways. International journal of molecular sciences. vol 25. issue 3. 2024-02-10. PMID:38339153. transcriptomics analysis and western blot assays were utilized to verify the molecular mechanism, rosavin (20, 40, and 80 mg/kg) dose-dependently ameliorated histopathological alterations, reduced the levels of inflammatory factors, and inhibited the tlr-4/nf-κb/mapk signaling pathway and apoptosis activation. 2024-02-10 2024-02-12 mouse
Jifang Liang, Junkun Zhang, Jixiu Fan, Shuxian Chen, Weidong W. ANXA3 interference inactivates ERK/ELK1 pathway to mitigate inflammation and apoptosis in sepsis-associated acute lung injury. Molecular immunology. vol 167. 2024-02-04. PMID:38310670. western blot analyzed the expression of proteins associated with inflammation, apoptosis and extracellular-signal-regulated kinase (erk)/ets-like gene 1 (elk1) signaling. 2024-02-04 2024-02-07 mouse
Feng Hu, Chaoyang Li. TRPM2 knockdown attenuates myocardial apoptosis and promotes autophagy in HFD/STZ-induced diabetic mice via regulating the MEK/ERK and mTORC1 signaling pathway. Molecular and cellular biochemistry. 2024-02-02. PMID:38308007. western blot, real-time quantitative pcr (rt-qpcr), immunohistochemistry and fluorescence, electron, cck-8, and flow cytometry were used to evaluate the phenotype of cardiac inflammation, fibrosis, apoptosis, and autophagy. 2024-02-02 2024-02-05 mouse
Yuzhou Xue, Yu Hu, Shikai Yu, Wenyan Zhu, Lin Liu, Minghao Luo, Suxin Luo, Jian Shen, Longxiang Huang, Jie Liu, Dingyi Lv, Wenming Zhang, Jingyu Wang, Xiang L. The lncRNA GAS5 upregulates ANXA2 to mediate the macrophage inflammatory response during atherosclerosis development. Heliyon. vol 10. issue 2. 2024-01-31. PMID:38293536. apoptosis and inflammatory markers in macrophages were detected by western blot. 2024-01-31 2024-02-02 Not clear
Ting Liu, Congjie Zhai, Bo Tian, Chao Li, Shuangshuang Han, Shihui Wang, Mingda Xuan, Dehua Liu, Yunxia Zhao, Hongyan Zhao, Weifang Yu, Jia Wan. Downregulation of Roundabout guidance receptor 2 suppresses hepatocellular carcinoma progression by interacting with Y-box binding protein 1. Scientific reports. vol 14. issue 1. 2024-01-31. PMID:38297025. then we established hepg2 and huh7 hepatoma cell lines with knock-down robo2 by transfection with lentiviral vectors, and examined the occurrence of emt, proliferation and apoptosis abilities in hcc cells by western blot, flow cytometry, wound healing assay and tunel staining. 2024-01-31 2024-02-03 human
Cian Zhang, Yongying Liu, Jing Zhao, Jingyu Xu, Bei Pei, Xuan Liu, Xiaoqiang Yu. KIAA0101 promotes cisplatin resistance through regulating cell apoptosis in lung cancer cells. Cellular and molecular biology (Noisy-le-Grand, France). vol 69. issue 14. 2024-01-27. PMID:38279448. western blot analysis was performed to measure the levels of p53-related apoptosis proteins. 2024-01-27 2024-01-29 human
Yulin Zhang, Hongxian Wu, Chaozhi Xu, Shanqian Li, Yue Hu, Zongyi Zhang, Guixian Wu, Yuling Liu, Lin Yang, Yue Huang, Wenjun Lu, Lina H. (-)-Epigallocatechin gallate alleviates chronic unpredictable mild stress-induced depressive symptoms in mice by regulating the mTOR autophagy pathway and inhibiting NLRP3 inflammasome activation. Food science & nutrition. vol 12. issue 1. 2024-01-25. PMID:38268911. then, elisa, western blot and qt-pcr tests were used to assess the expression of the key components of the nlrp3 inflammasome and its downstream inflammatory effectors (e.g., il-1β, il-18), autophagy markers (beclin-1, lc3, p62) and apoptosis markers (bax, bcl-2) in mouse brain tissues. 2024-01-25 2024-01-27 mouse
Yue Wu, Dan Chen, Longguang L. EXPRESS: Morinda officinalis polysaccharide promotes the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via microRNA-210-3p / scavenger receptor class A member 3. Journal of investigative medicine : the official publication of the American Federation for Clinical Research. 2024-01-24. PMID:38264863. cell counting kit-8 assay was employed to detect cell viability; terminal-deoxynucleoitidyl transferase mediated nick end labeling assay and flow cytometry were adopted to detect apoptosis; alkaline phosphatase (alp) activity assay kit was applied to detect alp activity; western blot was executed to quantify the expression levels of scara3, osteogenic and adipogenic differentiation markers. 2024-01-24 2024-01-26 human
Peipei Wang, Shitong Min, Congliang Chen, Junmei Hu, Dapeng Wei, Xia Wan. Phytohemagglutinin from Phaseolus vulgaris enhances the lung cancer cell chemotherapy sensitivity by changing cell membrane permeability. Journal of natural medicines. 2024-01-24. PMID:38265611. our results demonstrated that the pha significantly enhanced the sensitivity of lung cancer cells to ddp and adm, and western blot showed that pha combined with ddp or adm enhance cytotoxic effects by inhibiting autophagy and promoting apoptosis. 2024-01-24 2024-01-26 Not clear
XueFeng Feng, Lin Yang, Xiaojun Liu, Menghan Liu, Lu Liu, Jing Liu, JianMin Lu. Long non-coding RNA small nucleolar RNA host gene 29 drives chronic myeloid leukemia progression via microRNA-483-3p/Casitas B-lineage Lymphoma axis-mediated activation of the phosphoinositide 3-kinase/Akt pathway. Medical oncology (Northwood, London, England). vol 41. issue 2. 2024-01-22. PMID:38252204. western blot analysis was employed to assess protein expressions related to cellular proliferation, apoptosis, and oncogenesis. 2024-01-22 2024-01-24 human
Chandramohan Kiruthiga, Devasahayam Jaya Balan, Nagaiah Hari Prasath, Muthushanmugam Manikandakrishnan, Sakthivel Jafni, Narayanasamy Marimuthu Prabhu, Shunmugiah Karutha Pandian, Kasi Pandima Dev. Synergistic induction of apoptosis in lung cancer cells through co-delivery of PLGA phytol/α-bisabolol nanoparticles. Naunyn-Schmiedeberg's archives of pharmacology. 2024-01-19. PMID:38240783. apoptosis was confirmed through annexin v-fitc/pi staining and western blot analysis, which also revealed changes in bax and bcl-2 protein expression. 2024-01-19 2024-01-21 zebrafish
Chao Ma, Jizhi Zhao, Lian Zhou, Congwei Jia, Yanping Shi, Xing Li, Kedi Jihu, Tao Zhan. Targeting ENPP1 depletion may be a promising therapeutic strategy for treating oral squamous cell carcinoma via cytotoxic autophagy-related apoptosis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. vol 38. issue 2. 2024-01-17. PMID:38231531. cellular key proteins related to cell autophagy and apoptosis were evaluated by western blot and immunofluorescent staining. 2024-01-17 2024-01-19 mouse
Chongyun Wu, Qianting Deng, Ling Zhu, Timon Cheng-Yi Liu, Rui Duan, Luodan Yan. Methylene Blue Pretreatment Protects Against Repeated Neonatal Isoflurane Exposure-Induced Brain Injury and Memory Loss. Molecular neurobiology. 2024-01-17. PMID:38233687. immunofluorescence staining, f-jade c staining, tunel staining, and western blot analysis were performed to determine mitochondrial fragmentation, neuronal injury, degeneration, and apoptosis. 2024-01-17 2024-01-20 rat
Lin Bai, Yaping Zhang, Changmin Zheng, Shifu Xu, Yining He, Guangqiang Yu, Delun Huang, Yulin Huang, Mingxing Li, Changlong X. Tanshinone IIA protects mouse testes from heat stress injury by inhibiting apoptosis and TGFβ1/Smad2/Smad3 signaling pathway. Cell stress & chaperones. vol 28. issue 6. 2024-01-15. PMID:38225017. using western blot technology, we found that the hs group upregulated tgfβ1/smad2/smad3 pathway protein expression, causing cell apoptosis, testicular tissue organic lesions, and affecting testicular secretion function. 2024-01-15 2024-01-18 mouse