| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Lihong Zhang, Qingzi Guo, Ran An, Shuhan Shen, Lin Yi. In vitro ischemic preconditioning mediates the Ca Brain research. 2023-12-23. PMID:38141801. |
cell viability was detected by mtt, apoptosis by hoechst 33,258 staining, the levels of tnf-α and il-1β mrna by rt-qpcr and elisa, and the levels of can, nfat, cox-2 by rt-qpcr and western blot. |
2023-12-23 |
2023-12-25 |
Not clear |
| Mine Caglayan, Sibel Ozde. Potential impacts of bisphenols on prostate cells: An overview of cytotoxicity, proliferation, oxidative stress, apoptosis, and ER-stress response activation. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2023-12-22. PMID:38134982. |
pnt1a and pc-3 were treated with bpa, bpf and bps at concentrations of 0.1, 1 and 10 μm for 48 h cytotoxicity, brdu cell proliferation, ros generation, apoptosis detection, gene expression analysis and western blot analysis were performed. |
2023-12-22 |
2023-12-25 |
human |
| Ling-Ray Tai, Yi-Fen Chiang, Ko-Chieh Huang, Hsin-Yuan Chen, Mohamed Ali, Shih-Min Hsi. Hinokitiol as a modulator of TLR4 signaling and apoptotic pathways in atopic dermatitis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 170. 2023-12-21. PMID:38128179. |
additionally, the cell culture model enabled the evaluation of apoptosis and ros levels using mtt assay, crystal violet staining, western blot, and dcfda assays. |
2023-12-21 |
2023-12-24 |
Not clear |
| Gao Yuan, Ning Li, Xue Qing, Wu Lujia, Huang Kai, Xu Xiangyang, Yuan Ye, Li Qin, Liu Xiaohong, Han Li. BFGF attenuates aortic valvular interstitial cell calcification by inhibiting endoplasmic reticulum stress-mediated apoptosis. Experimental cell research. 2023-12-19. PMID:38113969. |
the protein expression level was detected by western blot, and apoptosis was determined by flow cytometry. |
2023-12-19 |
2023-12-23 |
rat |
| Ting-Ting Li, Jie Chen, En-Sheng Ji, Ya-Jing Gu. [Effect and mechanism of Danggui Buxue Decoction-containing serum in mitigating H9c2 cell injury caused by exposure to intermittent low oxygen]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 48. issue 21. 2023-12-19. PMID:38114184. |
western blot was employed to determine the expression levels of the autophagy-related proteins microtubule-associated proteins light chain 3Ⅱ(lc3Ⅱ), microtubule-associated proteins light chain 3Ⅰ(lc3Ⅰ), p62, parkin and apoptosis related proteins pro caspase-3, caspase-3, b-cell lymphoma-2(bcl-2), bcl-2-associated x(bax). |
2023-12-19 |
2023-12-23 |
Not clear |
| Xinru Yang, Jian Liang, Yue Shu, Linlin Wei, Cailing Wen, Hui Luo, Liqing Ma, Tian Qin, Bin Wang, Siyu Zeng, Ying Liu, Chun Zho. Asperosaponin VI facilitates the regeneration of skeletal muscle injury by suppressing GSK-3β-mediated cell apoptosis. Journal of cellular biochemistry. 2023-12-11. PMID:38079224. |
apoptosis and autophagy-related protein expression were evaluated t s by western blot, and muscle regeneration markers were quantified by quantitative polymerase chain reaction. |
2023-12-11 |
2023-12-17 |
mouse |
| Ramadan F Abdelaziz, Ahmed M Hussein, Mohamed H Kotob, Christina Weiss, Krzysztof Chelminski, Tamara Stojanovic, Christian R Studenik, Mohammed Auf. Enhancement of Radiation Sensitivity by Cathepsin L Suppression in Colon Carcinoma Cells. International journal of molecular sciences. vol 24. issue 23. 2023-12-09. PMID:38069428. |
in addition, the study conducted protein expression analysis by western blot and immunofluorescence assay, cytotoxicity mtt, and apoptosis assays. |
2023-12-09 |
2023-12-17 |
Not clear |
| Zhifang Tang, Jingyuan Li, Chuan L. Post-Transcriptional Regulator RBM47 Stabilizes FBXO2 mRNA to Advance Osteoarthritis Development: WGCNA Analysis and Experimental Validation. Biochemical genetics. 2023-12-09. PMID:38070024. |
functional analysis results from the mtt assay, flow cytometry, evaluation of ldh activities and inflammatory mediators, and western blot analysis of extracellular matrix (ecm) proteins, showed that rbm47 knockdown significantly alleviated inflammation, apoptosis, and ecm degradation in interleukin 1β (il-1β)-treated chondrocytes. |
2023-12-09 |
2023-12-17 |
Not clear |
| Yiting Hou, Lihua Dong, Lihua Ca. Saxagliptin reduces the injury of Alzheimer's disease cell model by down-regulating the expression of miR-483-5p. Cellular and molecular biology (Noisy-le-Grand, France). vol 69. issue 12. 2023-12-08. PMID:38063096. |
then the levels of mda, sod and gsh-px in each group were detected by elisa method; cell apoptosis was detected by flow cytometry; the protein expression levels of bax and bcl-2 were detected by western blot; the expression level of mir-483-5p was detected by rt-qpcr. |
2023-12-08 |
2023-12-17 |
Not clear |
| Daomin Lu, Yuzheng Yang, Wukai Ma, Xueming Yao, Yi Ling, Ying Huang, Qiuyi Wang, Chengyu Xia, Lei Ho. Stigmasterol Depresses the Proliferation and Facilitates the Apoptosis of Fibroblast-Like Synoviocytes via the PI3K/AKT Signaling Pathway in Collagen-Induced Arthritis Rats. Alternative therapies in health and medicine. 2023-12-08. PMID:38064612. |
in addition, we investigated the mechanism of stigmasterol inhibiting fls proliferation and promoting its apoptosis by qpcr, western blot, and immunofluorescence assays. |
2023-12-08 |
2023-12-17 |
rat |
| Xixi Li, Zhipeng Huang, Hua Chao, Xunxun Li. The mechanism of Semen Persicae-Flos Carthami in treating hypertrophic scar: A study based on network pharmacological analysis and in vitro experiments. Chemical biology & drug design. 2023-12-06. PMID:38056934. |
besides, a modified explant culture method was applied to separate primary hypertrophic scar fibroblasts (hsfs); mtt assay to detect cell viability of hsfs after treatment by sp-fc for 24 h; annexin v-fitc/pi double staining combined with flow cytometry to test apoptosis; western blot to check the protein expression level of p53; and real-time fluorescence quantitative pcr to determine mrna level of p53. |
2023-12-06 |
2023-12-10 |
Not clear |
| Yun Chen, Bocheng Zhang, Cui Zhong, Yuqing Zhou, Lei Xue, Chenhui Luo, Liang Yi, Qian Gong, Ying Lon. let-7g sensitized liver cancer cells to 5-fluorouracil by downregulating ABCC10 expression. Chemical biology & drug design. 2023-12-06. PMID:38054583. |
the levels of biomarkers related to cell cycle progression and apoptosis were detected by western blot assays. |
2023-12-06 |
2023-12-10 |
mouse |
| Yunchang Cao, Hongbo Wen, Chaoqun Leng, Shaolong Fen. MiR-29a mediates the apoptotic effects of TNF-α on endothelial cells through inhibiting PI3K/AKT/BCL-2 axis. Journal of biochemical and molecular toxicology. 2023-12-04. PMID:38047396. |
real-time quantitative pcr and western blot were performed to detect the levels of target rnas and proteins/their phosphorylation in huvecs. tnf-α could inhibit huvec proliferation and induce huvec apoptosis in a positive dose- and time-dependent manner, with a similar way of mir-29a upregulation, but no effects on mir-29b/c. |
2023-12-04 |
2023-12-10 |
human |
| Qin Liu, Zhen-Peng Niu, Kun Yang, Jing-Rui Song, Xue-Nai Wei, Yu-Bing Huang, Chun-Mao Yuan, Yan-Mei L. Synergistic combination of isogarcinol isolated from edible fruits of Garcinia multiflora and dexamethasone to overcome leukemia glucocorticoid resistance. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 170. 2023-12-01. PMID:38039755. |
furthermore, western blot analysis revealed that iso and dex inhibits the pi3k/akt/mtor signaling pathway and promotes the nuclear translocation of glucocorticoid receptor (gr), which activates target genes nr3c1 and tsc22d3, leading to apoptosis in jurkat cells. |
2023-12-01 |
2023-12-10 |
mouse |
| Sungryeal Kim, Jeong Mi Kim, Eun Jeong Jeon, Ji Won Kim, Mi Eun Choi, Jin-Mi Park, Jeong-Seok Cho. Supernatant of activated platelet-rich plasma rejuvenated aging-induced hyposalivation in mouse. Scientific reports. vol 13. issue 1. 2023-12-01. PMID:38040732. |
the decrease of senescence, apoptosis, and inflammation observed by western blot in 22 m + saprp group. |
2023-12-01 |
2023-12-10 |
mouse |
| Tong Wu, Yanan Sheng, Yu Tian, Changyuan Wan. Vitexin Regulates Heat Shock Protein Expression by Modulating ROS Levels Thereby Protecting against Heat-Stress-Induced Apoptosis. Molecules (Basel, Switzerland). vol 28. issue 22. 2023-11-25. PMID:38005362. |
therefore, in this study, we used caco-2 cells to establish a heat stress model and vitamin c as a positive control to investigate the regulatory effects of vitexin on heat-stress-induced apoptosis and the related mechanisms using cell counting kit-8, flow cytometry, real-time quantitative polymerase chain reaction, and western blot. |
2023-11-25 |
2023-11-28 |
human |
| Yiping Zhu, Junling Ren, Xu Wu, Yuan Zhang, Ying Wang, Jinwen Xu, Qiuyu Tan, Yuxin Jiang, Yulei L. lncRNA ENST00000422059 promotes cell proliferation and inhibits cell apoptosis in breast cancer by regulating the miR-145-5p/KLF5 axis. Acta biochimica et biophysica Sinica. 2023-11-24. PMID:37997376. |
we further verify that mir-145-5p regulated klf5 expression to promote cell apoptosis and inhibit cell proliferation in bc via dual-luciferase reporter assay, western blot analysis, qrt-pcr, cck-8 assay and cell apoptosis assay. |
2023-11-24 |
2023-11-29 |
Not clear |
| Yiping Zhu, Junling Ren, Xu Wu, Yuan Zhang, Ying Wang, Jinwen Xu, Qiuyu Tan, Yuxin Jiang, Yulei L. lncRNA ENST00000422059 promotes cell proliferation and inhibits cell apoptosis in breast cancer by regulating the miR-145-5p/KLF5 axis. Acta biochimica et biophysica Sinica. 2023-11-24. PMID:37997376. |
subsequently, fish, western blot analysis, qrt-pcr, cck-8 and cell apoptosis assays verified that enst00000422059 increases klf5 protein expression by sponging mirna to promote cell proliferation and inhibit cell apoptosis. |
2023-11-24 |
2023-11-29 |
Not clear |
| Meng Yuan, Asli F Ceylan, Rifeng Gao, Hong Zhu, Yingmei Zhang, Jun Re. Selective inhibition of the NLRP3 inflammasome protects against acute ethanol-induced cardiotoxicity in an FBXL2-dependent manner. Acta biochimica et biophysica Sinica. 2023-11-23. PMID:37994158. |
levels of nlrp3 inflammasome, mitophagy and apoptosis are evaluated by western blot analysis and immunofluorescence techniques. |
2023-11-23 |
2023-11-29 |
mouse |
| Jian Hao, Zhenhan Li, Li Xie, Bingbing Yu, Boyuan Ma, Yubiao Yang, Xuchen Ma, Bitao Wang, Xianhu Zho. Syringaresinol promotes the recovery of spinal cord injury by inhibiting neuron apoptosis via activating the ubiquitination factor E4B/AKT Serine/Threonine kinase signal pathway. Brain research. 2023-11-22. PMID:37992795. |
second, mtt was employed to evaluate cell proliferation rate, western blot was performed to detect protein expression, rt-qpcr was conducted to detect mrna expression levels, flow cytometry and 5-ethynyl-2'-deoxyuridine (edu) staining were used to determine cell apoptosis, and immunofluorescence and immunohistochemistry were used to estimate the expression of rna binding fox-1 homolog 3 and clipped caspase 3. |
2023-11-22 |
2023-11-29 |
rat |