| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| M Zanni, A Tamburro, I Santone, D Rotili. Modulation by transforming growth factor-beta 1 and interleukin-1 beta of proteoglycan release and chondrodisaccharide composition in porcine articular cartilage. Seminars in thrombosis and hemostasis. vol 20. issue 2. 1995-01-17. PMID:7997887. |
gags of conditioned medium and extracellular matrix proteoglycans were digested to about 90% by chondroitin abc lyase, suggesting that cs is the major gag present. |
1995-01-17 |
2023-08-12 |
Not clear |
| J L Fowlkes, K Suzuki, H Nagase, K M Thrailkil. Proteolysis of insulin-like growth factor binding protein-3 during rat pregnancy: a role for matrix metalloproteinases. Endocrinology. vol 135. issue 6. 1995-01-06. PMID:7527335. |
based on our previous findings that matrix metalloproteinases (mmps) degrade igfbp-3 in fibroblast conditioned media, we hypothesized that mmps might be involved in the degradation of igfbp-3 by rat pregnancy serum. |
1995-01-06 |
2023-08-12 |
human |
| B D Boyan, Z Schwartz, S Park-Snyder, D D Dean, F Yang, D Twardzik, L F Bonewal. Latent transforming growth factor-beta is produced by chondrocytes and activated by extracellular matrix vesicles upon exposure to 1,25-(OH)2D3. The Journal of biological chemistry. vol 269. issue 45. 1994-12-16. PMID:7961777. |
since active growth factor was not observed in the conditioned medium, we tested the hypothesis that latent tgf-beta could be activated in the matrix. |
1994-12-16 |
2023-08-12 |
Not clear |
| L C Smith-Thomas, J Fok-Seang, J Stevens, J S Du, E Muir, A Faissner, H M Geller, J H Rogers, J W Fawcet. An inhibitor of neurite outgrowth produced by astrocytes. Journal of cell science. vol 107 ( Pt 6). 1994-11-29. PMID:7962209. |
medium conditioned by the cell lines has the same properties as the matrix, since dorsal root ganglia (drgs) grown in conditioned medium from the neu7 line grow axons poorly, while drgs grown in medium conditioned by a7 or primary astrocytes grow many long axons. |
1994-11-29 |
2023-08-12 |
Not clear |
| J L Fowlkes, J J Enghild, K Suzuki, H Nagas. Matrix metalloproteinases degrade insulin-like growth factor-binding protein-3 in dermal fibroblast cultures. The Journal of biological chemistry. vol 269. issue 41. 1994-11-17. PMID:7523391. |
initial studies using ig-fbp-3-substrate zymography identified several igfbp-3-degrading proteases with m(r) 52,000-72,000, which were inhibitable by edta and were shifted to lower m(r) species after treatment of conditioned medium with an organomercurial, suggesting that they might represent one or more of the matrix metalloproteinases (mmps). |
1994-11-17 |
2023-08-12 |
human |
| L D Truong, M W Majesky, J Pindu. Tenascin is synthesized and secreted by rat mesangial cells in culture and is present in extracellular matrix in human glomerular diseases. Journal of the American Society of Nephrology : JASN. vol 4. issue 10. 1994-09-26. PMID:7520763. |
in this study, the synthesis of tn by cultured rat mesangial cells was documented by the following observations: (1) northern hybridization of total rna extracted from mesangial cells showed two distinct species of tn mrna; (2) immunoblotting of the protein extracted from the conditioned medium demonstrated four tn protein bands; (3) immunoblotting of the protein extracted from the mesangial cell lysate demonstrated at least four tn protein bands; and (4) immunohistochemical techniques identified tn within the cytoplasm of mesangial cells and in the surrounding extracellular matrix. |
1994-09-26 |
2023-08-12 |
human |
| F E Varzaneh, G Shillabeer, K L Wong, D C La. Extracellular matrix components secreted by microvascular endothelial cells stimulate preadipocyte differentiation in vitro. Metabolism: clinical and experimental. vol 43. issue 7. 1994-08-10. PMID:8028517. |
extracellular matrix components (ec) were prepared by differential centrifugation of medium conditioned by microvascular endothelial cells (cm). |
1994-08-10 |
2023-08-12 |
Not clear |
| M D Johnson, H R Kim, L Chesler, G Tsao-Wu, N Bouck, P J Polverin. Inhibition of angiogenesis by tissue inhibitor of metalloproteinase. Journal of cellular physiology. vol 160. issue 1. 1994-08-04. PMID:7517405. |
using an in vivo angiogenesis assay, the endothelial cell response to known angiogenic factors, basic fibroblast growth factor (bfgf) and adipocyte conditioned medium, was blocked by an inhibitor of matrix metalloproteinase activity, timp-1. |
1994-08-04 |
2023-08-12 |
Not clear |
| S Hyuga, Y Nishikawa, K Sakata, H Tanaka, S Yamagata, K Sugita, S Saga, M Matsuyama, S Shimiz. Autocrine factor enhancing the secretion of M(r) 95,000 gelatinase (matrix metalloproteinase 9) in serum-free medium conditioned with murine metastatic colon carcinoma cells. Cancer research. vol 54. issue 13. 1994-07-27. PMID:8012988. |
autocrine factor enhancing the secretion of m(r) 95,000 gelatinase (matrix metalloproteinase 9) in serum-free medium conditioned with murine metastatic colon carcinoma cells. |
1994-07-27 |
2023-08-12 |
Not clear |
| B P Himelstein, R Canete-Soler, E J Bernhard, R J Musche. Induction of fibroblast 92 kDa gelatinase/type IV collagenase expression by direct contact with metastatic tumor cells. Journal of cell science. vol 107 ( Pt 2). 1994-07-08. PMID:8207074. |
in addition, ref could not be stimulated to produce the 92 kda gelatinase by 2.8-derived conditioned medium, by 2.8-derived extracellular matrix, or by isolated matrix components. |
1994-07-08 |
2023-08-12 |
mouse |
| S J Braunhut, M A Mose. Retinoids modulate endothelial cell production of matrix-degrading proteases and tissue inhibitors of metalloproteinases (TIMP). The Journal of biological chemistry. vol 269. issue 18. 1994-06-09. PMID:8175780. |
substrate gel electrophoresis (zymography) revealed several major matrix metalloproteinases (mmp), of approximately 98-96, 72-68, and 46-45 kda, whose activities were altered in their amounts in the conditioned media (cm) of ec following retinol or retinoic acid treatment when compared to amounts detected in cm of control cells. |
1994-06-09 |
2023-08-12 |
Not clear |
| N Fujimoto, N Mouri, K Iwata, E Ohuchi, Y Okada, T Hayakaw. A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 2 (72-kDa gelatinase/type IV collagenase) using monoclonal antibodies. Clinica chimica acta; international journal of clinical chemistry. vol 221. issue 1-2. 1994-05-12. PMID:8149645. |
a one-step sandwich enzyme immunoassay (eia) for human matrix metalloproteinase 2 (mmp-2, 72-kda gelatinase/type iv collagenase, ec 3.4.24.24) was established with a pair of monoclonal antibodies prepared against the precursor form of mmp-2 (prommp-2) purified from the conditioned medium of human skin fibroblasts or against a synthetic peptide corresponding to the n-terminal domain of prommp-2. |
1994-05-12 |
2023-08-12 |
human |
| A Kennedy, R N Fran. Cultured ocular cells and extracellular matrices: role of growth factors, retinoic acid and cell polarity. Current eye research. vol 12. issue 8. 1993-12-21. PMID:8222729. |
however, in some experiments performed with lots of matrigel (a commercially available basement membrane extract from a murine tumor) obtained prior to mid-1991, media "conditioned" by incubation with this matrix did show enhanced ability to facilitate ec and rpe cell proliferation. |
1993-12-21 |
2023-08-12 |
cattle |
| D J Hill, A Logan, M Ong, D De Sousa, A M Gonzale. Basic fibroblast growth factor is synthesized and released by isolated ovine fetal growth plate chondrocytes: potential role as an autocrine mitogen. Growth factors (Chur, Switzerland). vol 6. issue 4. 1993-12-10. PMID:1340207. |
serum-free, conditioned culture medium, extracellular matrix and chondrocyte cytoplasm contained 52 +/- 2 pm/micrograms dna, 66 +/- 2 pm/micrograms dna and 22 +/- 3 pm/micrograms dna basic fgf, respectively (mean +/- s.e.m., n = 8 fetuses), for cells obtained from animals of 35-40 days' gestation when assessed by radioimmunoassay. |
1993-12-10 |
2023-08-11 |
Not clear |
| D J Hill, A Logan, M Ong, D De Sousa, A M Gonzale. Basic fibroblast growth factor is synthesized and released by isolated ovine fetal growth plate chondrocytes: potential role as an autocrine mitogen. Growth factors (Chur, Switzerland). vol 6. issue 4. 1993-12-10. PMID:1340207. |
the appearance of radiommunoassayable basic fgf in conditioned medium, extracellular matrix, and chondrocyte cytoplasm observed during culture was blocked by co-incubation with cycloheximide. |
1993-12-10 |
2023-08-11 |
Not clear |
| S Sumitani, S Kasayama, B Sat. A role for heparan sulfate in androgen-induced deoxyribonucleic acid synthesis of mouse mammary carcinoma (Shionogi carcinoma 115)-derived SC-3 cells. Endocrinology. vol 132. issue 3. 1993-04-01. PMID:8440181. |
during this early period, autocrine growth-promoting activity was found to be associated with cell surface and extracellular matrix, but not to be present in the conditioned medium. |
1993-04-01 |
2023-08-12 |
mouse |
| D I Israel, J Nove, K M Kerns, I K Moutsatsos, R J Kaufma. Expression and characterization of bone morphogenetic protein-2 in Chinese hamster ovary cells. Growth factors (Chur, Switzerland). vol 7. issue 2. 1992-12-15. PMID:1419071. |
bmp-2 associates with both the extracellular matrix and the surface of cho cells, which may in part account for the unequal levels of extracellular propeptide and mature forms of the molecule in the conditioned medium. |
1992-12-15 |
2023-08-11 |
human |
| O Corticchiato, J F Cajot, M Abrahamson, S J Chan, D Keppler, B Sorda. Cystatin C and cathepsin B in human colon carcinoma: expression by cell lines and matrix degradation. International journal of cancer. vol 52. issue 4. 1992-11-23. PMID:1399147. |
this activity was totally neutralized by recombinant cystatin c, suggesting a potential for interaction between released extracellular cathepsin b and cystatin c. in vitro assays of degradation of extracellular matrix showed that cysteine proteinase inhibitors could decrease matrix degradation induced by pepsin-activated conditioned media. |
1992-11-23 |
2023-08-11 |
human |
| T Sreenath, L M Matrisian, W Stetler-Stevenson, S Gattoni-Celli, R O Pozzatt. Expression of matrix metalloproteinase genes in transformed rat cell lines of high and low metastatic potential. Cancer research. vol 52. issue 18. 1992-10-08. PMID:1325287. |
conditioned medium from those cell lines was assayed by western blot analysis for the production of four separate matrix metalloproteinases to see whether a correlation could be found between protease expression and the metastatic phenotype. |
1992-10-08 |
2023-08-11 |
rat |
| N Pavloff, P W Staskus, N S Kishnani, S P Hawke. A new inhibitor of metalloproteinases from chicken: ChIMP-3. A third member of the TIMP family. The Journal of biological chemistry. vol 267. issue 24. 1992-09-25. PMID:1512267. |
in this paper, we report that of the three most abundant chimps, chimp-3 and chimp-a are extracellular matrix components, whereas chimp-2 is found in the media conditioned by the cells. |
1992-09-25 |
2023-08-11 |
chicken |