| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| G Calvaruso, M Carabillò, M Giuliano, M Lauricella, A D'Anneo, R Vento, G Tesorier. Sodium phenylbutyrate induces apoptosis in human retinoblastoma Y79 cells: the effect of combined treatment with the topoisomerase I-inhibitor topotecan. International journal of oncology. vol 18. issue 6. 2001-07-26. PMID:11351256. |
western blot analysis demonstrated that exposure of y79 cells to phenylbutyrate decreased the level of the antiapoptotic factor bcl-2 and induced the activation of caspase-3, a key enzyme in the execution phase of apoptosis. |
2001-07-26 |
2023-08-12 |
human |
| S Urayama, A Kawakami, T Nakashima, S Yamasaki, A Hida, H Ida, M Kamachi, H Nakamura, T Origuchi, K Migita, Y Kawabe, K Eguch. New disease-modifying antirheumatic drug 2 acetylthiomethyl-4-(4-methylphenyl)-4-oxobutanoic acid (KE-298) selectively augments activation-induced T cell death. The Journal of laboratory and clinical medicine. vol 138. issue 1. 2001-07-26. PMID:11433223. |
fas expression and caspase-3 activity in activated t cells were also examined by flow cytometry, and expression of fas ligand (fasl), bcl-2-related proteins, and x chromosome-linked inhibitor of apoptosis protein (xiap) was determined by western blot analysis. |
2001-07-26 |
2023-08-12 |
Not clear |
| J Dai, R Shen, M Sumitomo, J S Goldberg, Y Geng, D Navarro, S Xu, J A Koutcher, M Garzotto, C T Powell, D M Nanu. Tumor-suppressive effects of neutral endopeptidase in androgen-independent prostate cancer cells. Clinical cancer research : an official journal of the American Association for Cancer Research. vol 7. issue 5. 2001-07-19. PMID:11350908. |
analysis of the mechanisms of nep-induced growth suppression revealed that nep expression in wt-5 cells induced a 4-fold increase in the number of pc cells undergoing apoptosis, and increased the expression of p21 tumor suppressor gene protein and the level of unphosphorylated retinoblastoma protein as determined by western blot. |
2001-07-19 |
2023-08-12 |
mouse |
| E Engidawork, T Gulesserian, R Seidl, N Cairns, G Lube. Expression of apoptosis related proteins in brains of patients with Alzheimer's disease. Neuroscience letters. vol 303. issue 2. 2001-06-28. PMID:11311497. |
to further substantiate this hypothesis we investigated the expression of a recently identified apoptosis related proteins and other apoptosis regulators in frontal cortex and cerebellum of ad by western blot and enzyme-linked immunsorbent assay technique. |
2001-06-28 |
2023-08-12 |
caenorhabditis_elegans |
| K Dimas, C Demetzos, V Vaos, P Ioannidis, T Tranga. Labdane type diterpenes down-regulate the expression of c-Myc protein, but not of Bcl-2, in human leukemia T-cells undergoing apoptosis. Leukemia research. vol 25. issue 6. 2001-06-28. PMID:11337016. |
western blot analysis showed that of c-myc proto-oncogene levels were markedly reduced before massive apoptosis ensued in h33aj-ja1 and molt3 cells, while bcl-2 expression remained unaffected. |
2001-06-28 |
2023-08-12 |
human |
| B Montaner, R Pérez-Tomá. Prodigiosin-induced apoptosis in human colon cancer cells. Life sciences. vol 68. issue 17. 2001-06-14. PMID:11388704. |
dld-1 and sw-620 human colon adenocarcinoma cells, nrk and swiss-3t3 nonmalignant cells were assayed by the mtt assay, fragmentation pattern of dna, hoechst 33342 staining and study of parp cleavage by western blot, in order to characterize the prodigiosin-induced apoptosis. |
2001-06-14 |
2023-08-12 |
human |
| P E Mirkes, S A Little, C C Umpierr. Co-localization of active caspase-3 and DNA fragmentation (TUNEL) in normal and hyperthermia-induced abnormal mouse development. Teratology. vol 63. issue 3. 2001-06-07. PMID:11283970. |
previous work has shown that caspase-3 activation and dna fragmentation, two hallmarks of apoptosis, are induced in day 9 mouse embryos exposed to hyperthermia (43 degrees c); however, the methods used to assess caspase-3 activation (western blot) and dna fragmentation (gel electrophoresis) did not allow these apoptotic events to be localized to specific cells within the embryo. |
2001-06-07 |
2023-08-12 |
mouse |
| J G Seol, W H Park, E S Kim, C W Jung, J M Hyun, Y Y Lee, B K Ki. Potential role of caspase-3 and -9 in arsenic trioxide-mediated apoptosis in PCI-1 head and neck cancer cells. International journal of oncology. vol 18. issue 2. 2001-05-24. PMID:11172589. |
to address the mechanism of apoptosis, a western blot assay was performed, showing that bax was up-regulated without a change in bcl-2. |
2001-05-24 |
2023-08-12 |
mouse |
| C X Guo, J Ma, X C Zhou, Y X Li. Expression of HSP70-2 gene during germ cell apoptosis in rat unilateral cryptorchid testes. Archives of andrology. vol 46. issue 2. 2001-05-21. PMID:11297064. |
to investigate the role of hsp70-2 gene in germ cell apoptosis induced by heat stress, its expression changes were examined in rat normal and unilateral cryptorchid testes by using in situ hybridization, immunohistochemistry, and northern blot analysis techniques. |
2001-05-21 |
2023-08-12 |
rat |
| G W Wang, Z Zhou, J B Klein, Y J Kan. Inhibition of hypoxia/reoxygenation-induced apoptosis in metallothionein-overexpressing cardiomyocytes. American journal of physiology. Heart and circulatory physiology. vol 280. issue 5. 2001-05-17. PMID:11299233. |
in association with apoptosis, mitochondrial cytochrome c release, as determined by western blot, was observed to occur in nontransgenic cardiomyocytes. |
2001-05-17 |
2023-08-12 |
mouse |
| Z Zhou, S F Jia, M C Hung, E S Kleinerma. E1A sensitizes HER2/neu-overexpressing Ewing's sarcoma cells to topoisomerase II-targeting anticancer drugs. Cancer research. vol 61. issue 8. 2001-05-10. PMID:11309298. |
e1a-induced apoptosis was demonstrated by both flow cytometric analysis and western blot analysis using a poly(adp-ribose) polymerase antibody. |
2001-05-10 |
2023-08-12 |
human |
| A Lansiaux, W Laine, B Baldeyrou, C Mahieu, N Wattez, H Vezin, F J Martinez, A Piñeyro, C Baill. DNA topoisomerase II inhibition by peroxisomicine A(1) and its radical metabolite induces apoptotic cell death of HL-60 and HL-60/MX2 human leukemia cells. Chemical research in toxicology. vol 14. issue 1. 2001-04-26. PMID:11170504. |
the cytometry and western blot analyses reveal that peroxisomicine induces apoptosis in leukemia cells. |
2001-04-26 |
2023-08-12 |
human |
| E Engidawork, T Gulesserian, B C Yoo, N Cairns, G Lube. Alteration of caspases and apoptosis-related proteins in brains of patients with Alzheimer's disease. Biochemical and biophysical research communications. vol 281. issue 1. 2001-04-05. PMID:11178964. |
to further substantiate the role of apoptosis in ad, we investigated the levels of nine different proteins involved in apoptosis by western blot technique in frontal cortex and cerebellum of control and ad subjects. |
2001-04-05 |
2023-08-12 |
human |
| K H Choi, T I Kim, D L Chong, H Y Lee, D S Ha. Gentamicin induced apoptosis of renal tubular epithelial (LLC-PK1) cells. The Korean journal of internal medicine. vol 15. issue 3. 2001-03-22. PMID:11242810. |
however, the 15-day gm treatment did not cause a significant elevation in the expression of the 45 kd fas protein, the cell surface molecule that stimulates apoptosis, by western blot analysis. |
2001-03-22 |
2023-08-12 |
Not clear |
| P Mercié, F Bello. [Physicopathologic mechanisms and methods of analysis of cellular apoptosis]. La Revue de medecine interne. vol 22. issue 1. 2001-03-08. PMID:11218312. |
the methods of identification of the apoptosis pathways are: morphological modifications observed in microscopy, the evaluation of the difference of the mitochondrial membrane potential, the measurement of the devdase activity, the labelling of the phosphatidylserines by the annexin v on the cell surface, and the western blot allowing the identification of the activated caspases. |
2001-03-08 |
2023-08-12 |
human |
| M Artwohl, T Hölzenbein, L Wagner, A Freudenthaler, W Waldhäusl, S M Baumgartner-Parze. Levamisole induced apoptosis in cultured vascular endothelial cells. British journal of pharmacology. vol 131. issue 8. 2001-02-22. PMID:11139434. |
cells exposed (24 h) to levamisole (range: 0.5 - 2 mmol l(-1)) alone or in combination with antioxidants (10 mmol l(-1) glutathione or 5 mmol l(-1) n-acetylcysteine or 0.1 mmol l(-1) tocopherol) were evaluated for apoptosis ((3)h-thymidine assays, in situ staining), mrna/protein expression (northern/western blot), and proliferation ((3)h-thymidine incorporation). |
2001-02-22 |
2023-08-12 |
human |
| F Zhu, W Yan, Z L Zhao, Y B Chai, F Lu, Q Wang, W D Peng, A G Yang, C J Wan. Improved PCR-based subtractive hybridization strategy for cloning differentially expressed genes. BioTechniques. vol 29. issue 2. 2001-02-01. PMID:10948432. |
twenty-seven clones related to apoptosis were identified by reverse dot blot assay. |
2001-02-01 |
2023-08-12 |
human |
| F Zhu, W Yan, Z L Zhao, Y B Chai, F Lu, Q Wang, W D Peng, A G Yang, C J Wan. Improved PCR-based subtractive hybridization strategy for cloning differentially expressed genes. BioTechniques. vol 29. issue 2. 2001-02-01. PMID:10948432. |
apr-1, apr-2, apr-3 and tnf were reidentified by reverse dot blot, and it is suggested that they might be related to apoptosis. |
2001-02-01 |
2023-08-12 |
human |
| K C Wang, A L Cheng, S E Chuang, H C Hsu, I J S. Retinoic acid-induced apoptotic pathway in T-cell lymphoma: Identification of four groups of genes with differential biological functions. Experimental hematology. vol 28. issue 12. 2001-01-25. PMID:11146166. |
the cloned gene fragments were then analyzed and confirmed by northern blot analysis on h9 and sr786 cell lines.atra-induced apoptosis of t-cell lymphoma was protein synthesis-dependent. |
2001-01-25 |
2023-08-12 |
Not clear |
| Q N Diep, R M Touyz, E L Schiffri. Docosahexaenoic acid, a peroxisome proliferator-activated receptor-alpha ligand, induces apoptosis in vascular smooth muscle cells by stimulation of p38 mitogen-activated protein kinase. Hypertension (Dallas, Tex. : 1979). vol 36. issue 5. 2000-12-28. PMID:11082155. |
to clarify possible pathways of apoptosis, we evaluated the expression of phosphorylated p38 mitogen-activated protein kinases, bax, bcl-2, cytochrome c, and peroxisome proliferator-activated receptor-alpha (ppar-alpha) with western blot analysis. |
2000-12-28 |
2023-08-12 |
rat |