| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Wenhua Wu, Yaping Li, Xiaokang Wu, Junrong Liang, Weiming You, Xinyuan He, Qinhui Feng, Ting Li, Xiaoli Ji. Carnosic acid nanocluster-based framework combined with PD-1 inhibitors impeded tumorigenesis and enhanced immunotherapy in hepatocellular carcinoma. Functional & integrative genomics. vol 24. issue 1. 2024-01-05. PMID:38182693. |
the effects of ca-nbf and anti-pd-1 on cell proliferation, cell cycle, apoptosis, invasion, and migration were evaluated by mtt assay, flow cytometry, and scratch test. |
2024-01-05 |
2024-01-08 |
mouse |
| Qian Chen, Yunfeng Li, Tailiang Lu, Jihui Luo, Li Yang, Zheng Zhou, Zeyu Tian, Siwen Tan, Qi Li. miR-373 promotes invasion and metastasis of colorectal cancer cells via activating ERK/MAPK pathway. Scientific reports. vol 14. issue 1. 2024-01-03. PMID:38167930. |
biological functions such as cell proliferation, invasion and apoptosis are being detected by mtt, cck-8, transwell, cell cycle analysis, and flow cytometry experiments to verify the changes in the biological behavior of colon cancer cells after overexpression and interference of mir-373 in sw-480 cells and to explore the effects of mir-373 on cell proliferation, invasion, and apoptosis in colon cancer cells. |
2024-01-03 |
2024-01-06 |
human |
| Xiaopeng Peng, Haoyao Yuan, Guangtian Chen, Yuliang Guo, Qiuer Liang, Qiming Chen, Weidong Ca. Investigation on the effect of ulinastatin on the apoptosis of vascular smooth muscle cells in rats with aortic dissection based on the Sirt1/FoxO3a pathway. Cellular and molecular biology (Noisy-le-Grand, France). vol 69. issue 13. 2023-12-30. PMID:38158682. |
for this purpose a rat model of aortic dissection (ad) was constructed by giving drinking water containing 0.08% β-aminopropionitrile (bapn) to rats, he staining was used to observe the pathological changes of the aorta in ad rats; the diseased blood vessels of ad rats were taken for primary culture and passage of vsmcs, the morphology of vsmcs was observed, and vsmcs were identify with immunofluorescence staining; vsmcs were treated with culture media containing 0, 1000, 2000, 3000, 4000, 5000, 6000, 7000 u/ml ulinastatin, and mtt kit was used to determine the effect of ulinastatin on vsmc proliferation in ad rats; the vsmc of ad rats were divided into blank group (normal culture), ulinastatin group (medium containing 5000 u/ml ulinastatin), sirt1 inhibitor group (medium containing 1 μmol/l ex527), ulinastatin + sirt1 inhibitor group (medium containing 5000 u/ml ulinastatin, 1 μmol/l ex527), flow cytometry was used to detect the vsmc apoptosis in each group, wb was used to detect the expression of vsmc apoptosis-related proteins and sirt1/foxo3a pathway-related proteins in each group. |
2023-12-30 |
2024-01-05 |
rat |
| Ali Rajabi, Majid Nejati, Mina Homayoonfal, Abbas Arj, Zahra Sadat Razavi, Amirreza Ostadian, Bahareh Mohammadzadeh, Massoud Vosough, Merat Karimi, Neda Rahimian, Michael R Hamblin, Ali Arash Anoushirvani, Hamed Mirzae. Doxorubicin-loaded zymosan nanoparticles: Synergistic cytotoxicity and modulation of apoptosis and Wnt/β-catenin signaling pathway in C26 colorectal cancer cells. International journal of biological macromolecules. 2023-12-24. PMID:38143055. |
the effect of these agents on c26 cell viability was evaluated by mtt tests and the expression of genes connected with the wnt/β-catenin pathway and apoptosis were analyzed by rt-qpcr and western blotting. |
2023-12-24 |
2023-12-27 |
Not clear |
| Lihong Zhang, Qingzi Guo, Ran An, Shuhan Shen, Lin Yi. In vitro ischemic preconditioning mediates the Ca Brain research. 2023-12-23. PMID:38141801. |
cell viability was detected by mtt, apoptosis by hoechst 33,258 staining, the levels of tnf-α and il-1β mrna by rt-qpcr and elisa, and the levels of can, nfat, cox-2 by rt-qpcr and western blot. |
2023-12-23 |
2023-12-25 |
Not clear |
| Ling-Ray Tai, Yi-Fen Chiang, Ko-Chieh Huang, Hsin-Yuan Chen, Mohamed Ali, Shih-Min Hsi. Hinokitiol as a modulator of TLR4 signaling and apoptotic pathways in atopic dermatitis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 170. 2023-12-21. PMID:38128179. |
additionally, the cell culture model enabled the evaluation of apoptosis and ros levels using mtt assay, crystal violet staining, western blot, and dcfda assays. |
2023-12-21 |
2023-12-24 |
Not clear |
| Ting Ma, Xinguo Ge, Jie Zhu, Chengxin Song, Pinhao Wang, Jiali Ca. Dioscin Impedes Proliferation, Metastasis and Enhances Autophagy of Gastric Cancer Cells via Regulating the USP8/TGM2 Pathway. Molecular biotechnology. 2023-12-12. PMID:38085503. |
cell viability, proliferation, apoptosis and invasion were measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-h-tetrazolium bromide (mtt), 5-ethynyl-2'-deoxyuridine (edu), flow cytometry and transwell assays, respectively. |
2023-12-12 |
2023-12-17 |
Not clear |
| E H Ibrahim, M Y Alshahrani, H A Ghramh, A F El-Kott, M Kilany, K Morsy, R Taha, A A El-Mansi, M A Sayed, H C Chandramoorthy, A E Ahmed, H Alothaid, K A Khan, A M Eldi. Immunomodulatory and anti-cancer potential of cloves (Syzygium aromaticum) bud extract and its phytogenic silver nanoparticles. Journal of physiology and pharmacology : an official journal of the Polish Physiological Society. vol 74. issue 5. 2023-12-12. PMID:38085521. |
cae and cae + nanosilver were examined for their active biomolecules using ftir analysis, p53 contents using real-time pcr, apoptosis and cell cycle arrest power on ht-29 cancer cell line via flow cytometerty and immunomodulatory potential utilizing mtt assay. |
2023-12-12 |
2023-12-17 |
Not clear |
| Ramadan F Abdelaziz, Ahmed M Hussein, Mohamed H Kotob, Christina Weiss, Krzysztof Chelminski, Tamara Stojanovic, Christian R Studenik, Mohammed Auf. Enhancement of Radiation Sensitivity by Cathepsin L Suppression in Colon Carcinoma Cells. International journal of molecular sciences. vol 24. issue 23. 2023-12-09. PMID:38069428. |
in addition, the study conducted protein expression analysis by western blot and immunofluorescence assay, cytotoxicity mtt, and apoptosis assays. |
2023-12-09 |
2023-12-17 |
Not clear |
| Zhifang Tang, Jingyuan Li, Chuan L. Post-Transcriptional Regulator RBM47 Stabilizes FBXO2 mRNA to Advance Osteoarthritis Development: WGCNA Analysis and Experimental Validation. Biochemical genetics. 2023-12-09. PMID:38070024. |
functional analysis results from the mtt assay, flow cytometry, evaluation of ldh activities and inflammatory mediators, and western blot analysis of extracellular matrix (ecm) proteins, showed that rbm47 knockdown significantly alleviated inflammation, apoptosis, and ecm degradation in interleukin 1β (il-1β)-treated chondrocytes. |
2023-12-09 |
2023-12-17 |
Not clear |
| Xixi Li, Zhipeng Huang, Hua Chao, Xunxun Li. The mechanism of Semen Persicae-Flos Carthami in treating hypertrophic scar: A study based on network pharmacological analysis and in vitro experiments. Chemical biology & drug design. 2023-12-06. PMID:38056934. |
besides, a modified explant culture method was applied to separate primary hypertrophic scar fibroblasts (hsfs); mtt assay to detect cell viability of hsfs after treatment by sp-fc for 24 h; annexin v-fitc/pi double staining combined with flow cytometry to test apoptosis; western blot to check the protein expression level of p53; and real-time fluorescence quantitative pcr to determine mrna level of p53. |
2023-12-06 |
2023-12-10 |
Not clear |
| Panigrahi Chandan, Arora Dev, Devaraj Ezhilarasan, Karthik Shree Harin. Boldine Treatment Induces Cytotoxicity in Human Colorectal Carcinoma and Osteosarcoma Cells. Cureus. vol 15. issue 11. 2023-12-04. PMID:38046745. |
results the mtt assay, microscopic analysis, and staining showed that boldine had dose-dependent cytotoxic effects against hct-116 and saos-2 cell lines by inhibiting their proliferation, viability, and migration, and inducing ros-mediated apoptosis. |
2023-12-04 |
2023-12-10 |
human |
| Yunchang Cao, Hongbo Wen, Chaoqun Leng, Shaolong Fen. MiR-29a mediates the apoptotic effects of TNF-α on endothelial cells through inhibiting PI3K/AKT/BCL-2 axis. Journal of biochemical and molecular toxicology. 2023-12-04. PMID:38047396. |
in this study, mtt assay and flow cytometer analysis were employed respectively to determine the proliferation and apoptosis of human umbilical vascular endothelial cells (huvecs) under tnf-α exposure. |
2023-12-04 |
2023-12-10 |
human |
| Buket Altinok Gunes, Tulin Ozkan, Nurbanu Gonulkirmaz, Asuman Sungurogl. The evaluation of the anti-cancer effects of Anoectochilus roxburghii on hematological cancers in vitro. Medical oncology (Northwood, London, England). vol 41. issue 1. 2023-12-03. PMID:38044345. |
the spectrophotometric mtt assay and the flow cytometric annexin v staining were used to examine the viability and apoptosis of the cells, respectively. |
2023-12-03 |
2023-12-10 |
Not clear |
| Zhe Zhang, Qinghua Zhang, Yani Yu, Shan S. Epigallocatechin gallate inhibits ovarian cancer cell growth and induces cell apoptosis via activation of FOXO3A. In vitro cellular & developmental biology. Animal. 2023-12-01. PMID:38038884. |
to evaluate the in vitro inhibitory effects of egcg against ovarian cancer, mtt assay, colony formation assay, apoptosis assay, and wound healing assay, were performed. |
2023-12-01 |
2023-12-10 |
Not clear |
| Han Wang, Yonghao Xiao, Yuanguo Zhang, Zhu Meng, Chenyu Zhao, Fanshan Qiu, Chongchong Li, Zengguo Fen. Study on the effect of type III recombinant humanized collagen on human vascular endothelial cells. Tissue engineering. Part C, Methods. 2023-11-29. PMID:38019085. |
the effect of hcolii on proliferation of ea.hy926 cells was detected by mtt assay, the effect of hcolii on cell migration was investigated by scratch assay, the effect of hcoliii on cell cycle and apoptosis was detected by flow cytometry, the ability of hcolii to induce angiogenesis of ea.hy926 cells was evaluated by angiogenesis assay, and the effect of hcolii on vegf expression was detected by rt-pcr analysis. |
2023-11-29 |
2023-12-07 |
human |
| Jie Wang, Hao Yin, Wei Zhu, Qingyi He, Haitang Zhang, Lu Sun, Yunxiao Qiao, Yanwei Xian. Research on the resistance of isoviolanthin to hydrogen peroxide-triggered injury of skin keratinocytes based on Transcriptome sequencing and molecular docking. Medicine. vol 102. issue 47. 2023-11-28. PMID:38013320. |
in this study, methyl thiazolyl tetrazolium (mtt) assay and western blots were used to investigate the skin-protective effect of isoviolanthin, a di-c-glycoside derived from dendrobium officinale, on hydrogen peroxide (h2o2)-triggered apoptosis of skin keratinocytes. |
2023-11-28 |
2023-11-29 |
human |
| Muttiah Barathan, Naveen Kumar Hawala Shivashekaregowda, See Mee Hoong, Kumutha Malar Vellasamy, Jamuna Vadivel. Anticancer effect of aromatic isoniazid derivatives in human gastric adenocarcinoma cells. Toxicology and applied pharmacology. 2023-11-25. PMID:38007073. |
the cytotoxic effect of ithb4 has been determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) assay and further confirmed for anticancer properties by means of apoptosis, reactive oxygen species (ros), nuclear fragmentation, lactate dehydrogenase (ldh), caspases, cytokines and morphological including phenotypic changes of cells assay. |
2023-11-25 |
2023-11-28 |
human |
| Jian Hao, Zhenhan Li, Li Xie, Bingbing Yu, Boyuan Ma, Yubiao Yang, Xuchen Ma, Bitao Wang, Xianhu Zho. Syringaresinol promotes the recovery of spinal cord injury by inhibiting neuron apoptosis via activating the ubiquitination factor E4B/AKT Serine/Threonine kinase signal pathway. Brain research. 2023-11-22. PMID:37992795. |
second, mtt was employed to evaluate cell proliferation rate, western blot was performed to detect protein expression, rt-qpcr was conducted to detect mrna expression levels, flow cytometry and 5-ethynyl-2'-deoxyuridine (edu) staining were used to determine cell apoptosis, and immunofluorescence and immunohistochemistry were used to estimate the expression of rna binding fox-1 homolog 3 and clipped caspase 3. |
2023-11-22 |
2023-11-29 |
rat |
| Mussa Makran, Guadalupe Garcia-Llatas, Amparo Alegría, Antonio Cill. Ethylcoprostanol modulates colorectal cancer cell proliferation and mitigates cytotoxicity of cholesterol metabolites in non-tumor colon cells. Food & function. 2023-11-20. PMID:37982821. |
ethylcoprostanol reduced the tumor cell proliferation (mtt), showing in flow cytometry assays induction of apoptosis |
2023-11-20 |
2023-11-29 |
human |