| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Evelien Gellynck, Kjetil W Andressen, Béatrice Lintermans, Guy Haegeman, Finn O Levy, Peter Vanhoenacker, Kathleen Van Craenenbroec. Biochemical and pharmacological study of N-linked glycosylation of the human serotonin 5-HT₇a receptor. The FEBS journal. vol 279. issue 11. 2012-07-05. PMID:22448645. |
western blot analysis of hek293t cells transiently expressing the 5-ht(7(a)) receptor in the presence of tunicamycin gave rise to a band shift, indicating the existence of an n-glycosylated form of the 5-ht(7(a)) receptor. |
2012-07-05 |
2023-08-12 |
human |
| C Gómez, G G Curto, F C Baltanás, J Valero, E O'Shea, M I Colado, D Díaz, E Weruaga, J R Alons. Changes in the serotonergic system and in brain-derived neurotrophic factor distribution in the main olfactory bulb of pcd mice before and after mitral cell loss. Neuroscience. vol 201. 2012-05-14. PMID:22133893. |
studies of the amount of 5-ht and its metabolite, 5-hydroxyindole acetic acid (5-hiaa), were performed by means of high-performance liquid chromatography (hplc), and the relative amounts of brain-derived neurotrophin factor, bdnf, and its major receptor, tropomyosin-related kinase b (trkb), were measured by western blot. |
2012-05-14 |
2023-08-12 |
mouse |
| Shogo J Miyagi, Abby C Collie. The development of UDP-glucuronosyltransferases 1A1 and 1A6 in the pediatric liver. Drug metabolism and disposition: the biological fate of chemicals. vol 39. issue 5. 2011-12-30. PMID:21266593. |
the development of ugt1a1 and 1a6 was studied in 50 pediatric liver samples using bilirubin, serotonin activity assays, and western blot as well as pharmacokinetic scaling. |
2011-12-30 |
2023-08-12 |
Not clear |
| C Nothdurfter, S Tanasic, G Rammes, R Rupprech. Modulation of ligand-gated ion channels as a novel pharmacological principle. Pharmacopsychiatry. vol 44 Suppl 1. 2011-12-19. PMID:21544743. |
gradient fractions were assayed for antidepressant concentrations by hplc; 5-ht (3) receptor membrane distribution was determined by western blot. |
2011-12-19 |
2023-08-12 |
Not clear |
| Elena Magrini, Ildikò Szabò, Andrea Doni, Javier Cibella, Antonella Viol. Serotonin-mediated tuning of human helper T cell responsiveness to the chemokine CXCL12. PloS one. vol 6. issue 8. 2011-12-19. PMID:21853036. |
by real-time pcr, western blot analysis and electrophysiological patch clamp experiments, we demonstrate that the type 3 5-ht receptor (5-ht(3)) is functionally expressed in human primary t cells. |
2011-12-19 |
2023-08-12 |
human |
| Robert R Rozeske, Andrew K Evans, Matthew G Frank, Linda R Watkins, Christopher A Lowry, Steven F Maie. Uncontrollable, but not controllable, stress desensitizes 5-HT1A receptors in the dorsal raphe nucleus. The Journal of neuroscience : the official journal of the Society for Neuroscience. vol 31. issue 40. 2011-11-18. PMID:21976495. |
last, western blot analysis revealed that uncontrollable stress leads to desensitization rather than downregulation of drn 5-ht(1a) receptors. |
2011-11-18 |
2023-08-12 |
rat |
| Rachel A Hill, Simon S Murray, Paul G Halley, Michele D Binder, Sally J Martin, Maarten van den Buus. Brain-derived neurotrophic factor expression is increased in the hippocampus of 5-HT(2C) receptor knockout mice. Hippocampus. vol 21. issue 4. 2011-09-28. PMID:20087884. |
western blot analysis demonstrated a significant 2.2-fold increase in the expression of the mature form of bdnf in 5-ht(2c) ko mice when compared with wild-type controls (wt) in the hippocampus (p = 0.008), but not frontal cortex or striatum. |
2011-09-28 |
2023-08-12 |
mouse |
| Jose G Lopez-Lopez, Javier Moral-Sanz, Giovanna Frazziano, Maria J Gomez-Villalobos, Laura Moreno, Carmen Menendez, Jorge Flores-Hernandez, Jose A Lorente, Angel Cogolludo, Francisco Perez-Vizcain. Type 1 diabetes-induced hyper-responsiveness to 5-hydroxytryptamine in rat pulmonary arteries via oxidative stress and induction of cyclooxygenase-2. The Journal of pharmacology and experimental therapeutics. vol 338. issue 1. 2011-08-30. PMID:21521772. |
after 6 weeks, diabetic animals showed a down-regulation of the lung bone morphogenetic protein receptor type 2, up-regulation of 5-hydroxytryptamine (5-ht) 2a receptors and cyclooxygenase-2 (cox-2) proteins as measured by western blot analysis, and increased contractile responses to 5-ht in isolated intrapulmonary arteries. |
2011-08-30 |
2023-08-12 |
rat |
| Yun Li, Xiao-Feng Li, Hua Guo, Jing-Dong Xu, Xiao-Hui Zhang, Li-Sheng Li, Xiao-Yan Feng, Yue Zhang, Zhong-Ping Duan, Jin-Xia Zh. Colonic submucosal 5-HT(3) receptor-mediated somatostatin-dependent secretoinhibitory pathway is suppressed in water-immersion restraint stressed rats. European journal of pharmacology. vol 656. issue 1-3. 2011-06-13. PMID:21291881. |
real-time pcr and western blot were performed to study the expression of the 5-ht(3) receptor, 5-ht(4) receptor, and somatostatin receptor 2. |
2011-06-13 |
2023-08-12 |
rat |
| Elisabeth A Riksen, Astrid K Stunes, Anne Kalvik, Björn I Gustafsson, Malcolm L Snead, Unni Syversen, Ståle P Lyngstadaas, Janne E Reselan. Serotonin and fluoxetine receptors are expressed in enamel organs and LS8 cells and modulate gene expression in LS8 cells. European journal of oral sciences. vol 118. issue 6. 2011-03-24. PMID:21083617. |
using rt-pcr and western blot analysis we found that enamel organs from 3-d-old mice and ameloblast-like cells (ls8 cells) express functional serotonin receptors, the rate-limiting enzyme in serotonin synthesis (thp1), as well as the serotonin transporter (5htt), indicating that enamel organs and ameloblasts are able to respond to serotonin and regulate serotonin availability. |
2011-03-24 |
2023-08-12 |
mouse |
| Noelle C Anastasio, Maria Fe Lanfranco, Marcy J Bubar, Patricia K Seitz, Sonja J Stutz, Andrew G McGinnis, Cheryl S Watson, Kathryn A Cunningha. Serotonin 5-HT2C receptor protein expression is enriched in synaptosomal and post-synaptic compartments of rat cortex. Journal of neurochemistry. vol 113. issue 6. 2010-08-06. PMID:20345755. |
utilizing multiples tissues (brain, peripheral tissues), protein fractions (synaptosomal, post-synaptic density), and controls (peptide neutralization, 5-ht(2c)r stably-expressing cells), we established the selectivity of two commercially available 5-ht(2c)r antibodies and employed the antibodies in western blot and immunoprecipitation studies of prefrontal cortex (pfc) and motor cortex, two regions implicated in cognitive, emotional and motor dysfunction. |
2010-08-06 |
2023-08-12 |
rat |
| T Oufkir, M Arseneault, J T Sanderson, C Vaillancour. The 5-HT 2A serotonin receptor enhances cell viability, affects cell cycle progression and activates MEK-ERK1/2 and JAK2-STAT3 signalling pathways in human choriocarcinoma cell lines. Placenta. vol 31. issue 5. 2010-08-03. PMID:20338635. |
we also investigated, by co-immunoprecipitation and western blot analysis, the involvement of the mek-erk1/2 and jak2-stat3 signalling pathways following activation of the placental 5-ht(2a) receptor. |
2010-08-03 |
2023-08-12 |
human |
| Gino Giannaccini, Laura Betti, Lionella Palego, Lara Schmid, Laura Fabbrini, Caterina Pelosini, Claudia Gargini, Ylenia Da Valle, Mario Lanza, Alessandro Marsili, Margherita Maffei, Ferruccio Santini, Paolo Vitti, Aldo Pinchera, Antonio Lucacchin. Human serotonin transporter expression during megakaryocytic differentiation of MEG-01 cells. Neurochemical research. vol 35. issue 4. 2010-08-02. PMID:20041293. |
cells were cultured for 8 days with 10(-7)m 4-beta-12-tetradecanoylphorbol-13-acetate (beta-tpa) in the presence of 10% fetal bovine serum (fbs) and sert was assessed by real time pcr, immunofluorescence microscopy, western blot and [(3)h]5-ht re-uptake. |
2010-08-02 |
2023-08-12 |
human |
| Victor V Uteshev, Srinivasan Tupal, Yashanad Mhaskar, Carl L Faingol. Abnormal serotonin receptor expression in DBA/2 mice associated with susceptibility to sudden death due to respiratory arrest. Epilepsy research. vol 88. issue 2-3. 2010-05-03. PMID:20018491. |
the present study compared the expression of 5-ht subtype receptor proteins in excised brainstem tissue, containing the rostral ventral medulla respiratory region in dba/2 mice vs. seizure-resistant c57bl/6j mice, using western blot analysis. |
2010-05-03 |
2023-08-12 |
mouse |
| Hong-Yu Liu, Lu-Qing Pan, De-Bin Zhen. Effects of injection of biogenic amines on expression of gill related ion transporter mRNA and alpha-subunit protein in Litopenaeus vannamei. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. vol 154. issue 1. 2009-09-11. PMID:19176220. |
western blot and immunocytochemistry analysis were employeed to investigate the effects of biogenic amines such as dopamine (da) and 5-hydroxytryptamine (5-ht) injection on expression of protein abundance and immunostained sites. |
2009-09-11 |
2023-08-12 |
Not clear |
| Zhi Li, Xiao-Jun Zhang, Hong-xi Xu, Joseph J Y Sung, Zhao-xiang Bia. Intracolonical administration of protease-activated receptor-2 agonists produced visceral hyperalgesia by up-regulating serotonin in the colon of rats. European journal of pharmacology. vol 606. issue 1-3. 2009-09-01. PMID:19374846. |
the enterochromaffin (ec) cell number, 5-ht content and tryrptophan hydroxylase (tph) protein expression were detected with immunohistochemistry, fluorescent measurement and western blot analysis. |
2009-09-01 |
2023-08-12 |
rat |
| J A Henderson, C L Bethe. Differential effects of ovarian steroids and raloxifene on serotonin 1A and 2C receptor protein expression in macaques. Endocrine. vol 33. issue 3. 2009-06-19. PMID:19021000. |
to further understand the role of ovarian hormones in the function of the serotonin neural system, we investigated the effects of estradiol (e), progesterone (p), and raloxifene on 5ht 1a and 2c receptor protein expression in the dorsal raphe region using western blot analysis. |
2009-06-19 |
2023-08-12 |
monkey |
| R C Shelton, E Sanders-Bush, D H Manier, D A Lewi. Elevated 5-HT 2A receptors in postmortem prefrontal cortex in major depression is associated with reduced activity of protein kinase A. Neuroscience. vol 158. issue 4. 2009-05-20. PMID:19111907. |
levels of 5-ht(1a), 5-ht(2a), and 5-ht(2c) receptor were quantitated via western blot analyses. |
2009-05-20 |
2023-08-12 |
human |
| K E Borgmann-Winter, N E Rawson, H-Y Wang, H Wang, M L Macdonald, M H Ozdener, K K Yee, G Gomez, J Xu, B Bryant, G Adamek, N Mirza, E Pribitkin, C-G Hah. Human olfactory epithelial cells generated in vitro express diverse neuronal characteristics. Neuroscience. vol 158. issue 2. 2009-04-15. PMID:18996445. |
western blot analysis revealed the expression of neurotransmitter receptors for dopamine (d2r), 5-ht (5ht2c) and nmda subtypes 1 and 2a/2b. |
2009-04-15 |
2023-08-12 |
human |
| Nicolas Desbuards, Daniel Antier, Gael Y Rochefort, Coralie S Apfeldorfer, Emanuel Schenck, Gilles Hanton, Jean-Marc Hyveli. Dexfenfluramine discontinuous treatment does not worsen hypoxia-induced pulmonary vascular remodeling but activates RhoA/ROCK pathway: consequences on pulmonary hypertension. European journal of pharmacology. vol 602. issue 2-3. 2009-03-30. PMID:19049806. |
western blot, rt-pcr and immunofluorescence analysis revealed a greater expression of 5-ht transporter and rock, as well as a greater activation of rhoa in dexfenfluramine-hypoxic rats compared to hypoxic rats. |
2009-03-30 |
2023-08-12 |
rat |