| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Xiaolong Jiang, Guoqiang Xing, Chunhui Yang, Ajay Verma, Lei Zhang, He L. Stress impairs 5-HT2A receptor-mediated serotonergic facilitation of GABA release in juvenile rat basolateral amygdala. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology. vol 34. issue 2. 2009-02-23. PMID:18536707. |
quantitative rt-pcr and western blot analysis showed that the impairment of 5-ht(2a) receptor signaling primarily resulted from receptor downregulation. |
2009-02-23 |
2023-08-12 |
rat |
| Ju Shi, Michelle Landry, Gonzalo A Carrasco, George Battaglia, Nancy A Mum. Sustained treatment with a 5-HT(2A) receptor agonist causes functional desensitization and reductions in agonist-labeled 5-HT(2A) receptors despite increases in receptor protein levels in rats. Neuropharmacology. vol 55. issue 5. 2009-01-15. PMID:18588902. |
in contrast, western blot analysis demonstrated a significant increase in 5-ht(2a) receptor protein levels with 4 or 7 days of doi treatment to 167% and 191% of control levels, respectively. |
2009-01-15 |
2023-08-12 |
rat |
| Ju Shi, Michelle Landry, Gonzalo A Carrasco, George Battaglia, Nancy A Mum. Sustained treatment with a 5-HT(2A) receptor agonist causes functional desensitization and reductions in agonist-labeled 5-HT(2A) receptors despite increases in receptor protein levels in rats. Neuropharmacology. vol 55. issue 5. 2009-01-15. PMID:18588902. |
taken together, the 5-ht(2a) receptor-stimulated hormone responses, agonist binding data and western blot data suggest that although agonist treatment increases the levels of 5-ht(2a) receptor protein in the cell membrane, there is a reduction in the population of 5-ht(2a) receptors capable of high-affinity binding and mediating a functional response. |
2009-01-15 |
2023-08-12 |
rat |
| A M Harriott, M S Gol. Serotonin type 1D receptors (5HTR) are differentially distributed in nerve fibres innervating craniofacial tissues. Cephalalgia : an international journal of headache. vol 28. issue 9. 2008-12-03. PMID:18557979. |
the density of 5ht(1d)r was quantified in tissues obtained from adult female rats with western blot analysis. |
2008-12-03 |
2023-08-12 |
rat |
| G-L Chen, G M Mille. Rhesus monkey tryptophan hydroxylase-2 coding region haplotypes affect mRNA stability. Neuroscience. vol 155. issue 2. 2008-12-03. PMID:18593594. |
the levels of mtph2 mrna and protein were assessed by quantitative real-time pcr and western blot, respectively, while the intracellular 5-ht was measured by enzyme-linked immunosorbent assay (elisa). |
2008-12-03 |
2023-08-12 |
monkey |
| H Nguyen, H Wang, T le, W Ho, K A Sharkey, M G Swai. Downregulated hypothalamic 5-HT3 receptor expression and enhanced 5-HT3 receptor antagonist-mediated improvement in fatigue-like behaviour in cholestatic rats. Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society. vol 20. issue 3. 2008-03-31. PMID:17919312. |
a significant decrease in hypothalamic 5-ht(3) receptor expression was detected by immunohistochemistry and western blot in bdr vs sham rats, coupled with increased hypothalamic serotonin turnover identified by an elevated 5-hydroxyindoleacetic acid (5-hiaa) to 5-ht ratio in bdr vs sham rats. |
2008-03-31 |
2023-08-12 |
rat |
| Stephen J Kish, Junchao Tong, Oleh Hornykiewicz, Ali Rajput, Li-Jan Chang, Mark Guttman, Yoshiaki Furukaw. Preferential loss of serotonin markers in caudate versus putamen in Parkinson's disease. Brain : a journal of neurology. vol 131. issue Pt 1. 2008-03-06. PMID:17956909. |
we measured levels of sert immunoreactivity, and for comparison, protein levels of tryptophan hydroxylase (tph; the marker synthetic enzyme) using a western blot procedure, as well as concentrations of serotonin, its metabolite 5-hydroxyindoleacetic acid (5-hiaa), and dopamine by hplc in post-mortem striatum of patients with pd and normal controls. |
2008-03-06 |
2023-08-12 |
Not clear |
| Michael W McLane, George Hatzidimitriou, Jie Yuan, Una McCann, George Ricaurt. Heating induces aggregation and decreases detection of serotonin transporter protein on western blots. Synapse (New York, N.Y.). vol 61. issue 10. 2007-10-31. PMID:17623834. |
in recent years, there has been growing interest in the use of western blot analysis to monitor changes in the abundance of the serotonin transporter (sert) protein. |
2007-10-31 |
2023-08-12 |
Not clear |
| Benita Sjögren, Mark W Hamblin, Per Svenningsso. Cholesterol depletion reduces serotonin binding and signaling via human 5-HT(7(a)) receptors. European journal of pharmacology. vol 552. issue 1-3. 2007-01-04. PMID:17064686. |
cholesterol depletion also reduced the total level of 5-ht(7) receptor protein determined by western blot analysis. |
2007-01-04 |
2023-08-12 |
human |
| Carmen Mannucci, Michele Tedesco, Maria Bellomo, Achille P Caputi, Gioacchino Calapa. Long-term effects of nicotine on the forced swimming test in mice: an experimental model for the study of depression caused by smoke. Neurochemistry international. vol 49. issue 5. 2006-10-18. PMID:16697079. |
western blot analysis showed a great reduction of 5-ht(1a) receptor expression in nicotine mice measured at t=0 (last day of treatment) and at 15 and 30 days after nicotine withdrawal compared to control mice. |
2006-10-18 |
2023-08-12 |
mouse |
| Amy R Simon, Mariano Severgnini, Satoe Takahashi, Liliana Rozo, Bathsheeba Andrahbi, Abena Agyeman, Brent H Cochran, Regina M Day, Barry L Fanbur. 5-HT induction of c-fos gene expression requires reactive oxygen species and Rac1 and Ras GTPases. Cell biochemistry and biophysics. vol 42. issue 3. 2005-10-27. PMID:15976459. |
here, we show by western blot that 5-ht upregulates c-fos, an immediate early gene product known to regulate the entrance of quiescent cells into the cell cycle. |
2005-10-27 |
2023-08-12 |
cattle |
| Alexander D Dmitriev, Magnolia I Factor, Olga L Segal, Elena V Pavlova, Yulia S Massino, Maria B Smirnova, Dinora A Yakovleva, Dmitriy A Dmitriev, Elena A Kizim, Galina I Kolyaskina, Oleg S Bruso. Western blot analysis of human and rat serotonin transporter in platelets and brain using site-specific antibodies: evidence that transporter undergoes endoproteolytic cleavage. Clinica chimica acta; international journal of clinical chemistry. vol 356. issue 1-2. 2005-08-25. PMID:15936305. |
western blot analysis of human and rat serotonin transporter in platelets and brain using site-specific antibodies: evidence that transporter undergoes endoproteolytic cleavage. |
2005-08-25 |
2023-08-12 |
human |
| Richard B Rothman, Subramaniam Jayanthi, Jean L Cadet, Xiaoying Wang, Christina M Dersch, Michael H Bauman. Substituted amphetamines that produce long-term serotonin depletion in rat brain ("neurotoxicity") do not decrease serotonin transporter protein expression. Annals of the New York Academy of Sciences. vol 1025. 2005-02-18. PMID:15542713. |
despite the large decreases in sert binding and depletion of tissue 5-ht that occurred with d-fen administration, sert protein expression, as determined by western blot analysis, did not change in either tissue or time point. |
2005-02-18 |
2023-08-12 |
rat |
| Cécile Mahé, Michel Bernhard, Ionel Bobirnac, Corinna Keser, Erika Loetscher, Dominik Feuerbach, Kumlesh K Dev, Philippe Schoeffte. Functional expression of the serotonin 5-HT7 receptor in human glioblastoma cell lines. British journal of pharmacology. vol 143. issue 3. 2005-01-18. PMID:15339860. |
the aim of the present study was to assess the expression of native 5-ht(7) receptors and 5-ht(7) receptor mrna in a number of human glioblastoma cell lines, by means of camp measurements, western blot analysis and reverse transcriptase-polymerase chain reaction (rt-pcr) analysis. |
2005-01-18 |
2023-08-12 |
human |
| Cécile Mahé, Michel Bernhard, Ionel Bobirnac, Corinna Keser, Erika Loetscher, Dominik Feuerbach, Kumlesh K Dev, Philippe Schoeffte. Functional expression of the serotonin 5-HT7 receptor in human glioblastoma cell lines. British journal of pharmacology. vol 143. issue 3. 2005-01-18. PMID:15339860. |
western blot analysis revealed the presence of immunoreactive bands corresponding to the human 5-ht(7) receptor in extracts of all human glioblastoma cell lines. |
2005-01-18 |
2023-08-12 |
human |
| Raquel Hernanz, Maria J Alonso, Ana M Briones, Elisabet Vila, Ulf Simonsen, Mercedes Salaice. Mechanisms involved in the early increase of serotonin contraction evoked by endotoxin in rat middle cerebral arteries. British journal of pharmacology. vol 140. issue 4. 2004-11-09. PMID:14534151. |
the present study investigated the mechanisms involved in the increased 5-hydroxytryptamine (5-ht) vasoconstriction observed in rat middle cerebral arteries exposed in vitro to lipopolysaccharide (lps, 10 microg x ml-1) for 1-5 h. functional, immunohistochemical and western blot analysis and superoxide anion measurements by ethidium fluorescence were performed. |
2004-11-09 |
2023-08-12 |
rat |
| Isabelle Cloëz-Tayarani, Anne-France Petit-Bertron, Homer D Venters, Jean-Marc Cavaillo. Differential effect of serotonin on cytokine production in lipopolysaccharide-stimulated human peripheral blood mononuclear cells: involvement of 5-hydroxytryptamine2A receptors. International immunology. vol 15. issue 2. 2004-01-06. PMID:12578853. |
accordingly, we detected the presence of 5-ht(2a) receptors in pbmc by western blot analysis. |
2004-01-06 |
2023-08-12 |
human |
| Richard B Rothman, Subramaniam Jayanthi, Xiaoying Wang, Christina M Dersch, Jean L Cadet, Thomas Prisinzano, Kenner C Rice, Michael H Bauman. High-dose fenfluramine administration decreases serotonin transporter binding, but not serotonin transporter protein levels, in rat forebrain. Synapse (New York, N.Y.). vol 50. issue 3. 2004-01-06. PMID:14515341. |
despite the large decreases in sert binding and depletion of tissue 5-ht that occurred with d-fen administration, sert protein expression, as determined by western blot analysis, did not change in either tissue or time point. |
2004-01-06 |
2023-08-12 |
rat |
| Robert Meller, Joseph M Babity, David G Grahame-Smit. 5-HT2A receptor activation leads to increased BDNF mRNA expression in C6 glioma cells. Neuromolecular medicine. vol 1. issue 3. 2003-01-28. PMID:12095161. |
however, because it is unclear whether 5-ht manipulations directly affect bdnf expression, we examined bdnf mrna levels in c6 glioma cells following incubation with 5-ht using reverse transcription polymerase chain reaction (rt-pcr) and northern blot analysis. |
2003-01-28 |
2023-08-12 |
Not clear |
| Adamantios Serafeim, Gillian Grafton, Anita Chamba, Christopher D Gregory, Randy D Blakely, Norman G Bowery, Nicholas M Barnes, John Gordo. 5-Hydroxytryptamine drives apoptosis in biopsylike Burkitt lymphoma cells: reversal by selective serotonin reuptake inhibitors. Blood. vol 99. issue 7. 2002-05-02. PMID:11895792. |
western blot analysis showed that bl cells expressed protein for the 5-ht transporter, and transport assays confirmed active uptake of serotonin by the cells. |
2002-05-02 |
2023-08-12 |
Not clear |