| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| S A Monk, K Desai, C A Brady, J M Williams, L Lin, A Princivalle, A G Hope, N M Barne. Generation of a selective 5-HT3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in rat hippocampus. Neuropharmacology. vol 41. issue 8. 2002-03-26. PMID:11747906. |
western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-ht(3a)) or heteromeric (h5-ht(3a/3b)) receptors, as well as immunocytochemical studies with the same cell lines, indicated that ap86/3 recognised, selectively, the 5-ht(3b) subunit. |
2002-03-26 |
2023-08-12 |
rat |
| D Hranilović, C M Herak-Kramberger, L Cicin-Sain, I Sabolić, B Jerne. Serotonin transporter in rat platelets. Level of protein expression underlies inherited differences in uptake kinetics. Life sciences. vol 69. issue 1. 2001-07-05. PMID:11411805. |
searching for the basis of these differences, we performed quantitative western blot analysis of serotonin transporter (5htt) in platelet membranes isolated from both rat sublines. |
2001-07-05 |
2023-08-12 |
rat |
| H Kawano, H Tsuji, H Nishimura, S Kimura, S Yano, N Ukimura, Y Kunieda, M Yoshizumi, T Sugano, K Nakagawa, H Masuda, S Sawada, M Nakagaw. Serotonin induces the expression of tissue factor and plasminogen activator inhibitor-1 in cultured rat aortic endothelial cells. Blood. vol 97. issue 6. 2001-04-26. PMID:11238110. |
endothelial cells were stimulated with various concentrations of 5-ht (0.1 approximately 10 microm), and the expressions of tissue factor (tf), tissue factor pathway inhibitor (tfpi), plasminogen activator inhibitor-1 (pai-1), and tissue-type plasminogen activator (tpa) messenger rnas (mrnas) were evaluated by northern blot analysis. |
2001-04-26 |
2023-08-12 |
rat |
| W G Gallardo, A Hagiwara, K Hara, K Soyano, T W Snel. GABA, 5-HT and amino acids in the rotifers Brachionus plicatilis and Brachionus rotundiformis. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. vol 127. issue 3. 2001-02-01. PMID:11118940. |
in the present study, the endogenous presence of gaba and 5-ht in the rotifers b. plicatilis (nh3l and kamiura strains) and brachionus rotundiformis (langkawi strain) were confirmed by dot blot immunoassay and high-performance liquid chromatography (hplc). |
2001-02-01 |
2023-08-12 |
Not clear |
| P C Guest, K Salim, H A Skynner, S E George, J N Bresnick, G McAlliste. Identification and characterization of a truncated variant of the 5-hydroxytryptamine(2A) receptor produced by alternative splicing. Brain research. vol 876. issue 1-2. 2000-10-18. PMID:10973616. |
western blot analysis of hek-293 cells transfected transiently with a 5-ht(2a-tr) construct showed that a 30-kda protein was expressed on cell membranes. |
2000-10-18 |
2023-08-12 |
human |
| K R Oliver, A M Kinsey, A Wainwright, D J Sirinathsinghj. Localization of 5-ht(5A) receptor-like immunoreactivity in the rat brain. Brain research. vol 867. issue 1-2. 2000-08-04. PMID:10837806. |
in this study, antibodies raised to a 5-ht(5a)-specific peptide were characterized using dot blot, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) and immunohistochemistry, and the distribution of 5-ht(5a)-like immunoreactive material determined in rat brain. |
2000-08-04 |
2023-08-12 |
rat |
| N Z Lu, T A Shlaes, C Gundlah, S E Dziennis, R E Lyle, C L Bethe. Ovarian steroid action on tryptophan hydroxylase protein and serotonin compared to localization of ovarian steroid receptors in midbrain of guinea pigs. Endocrine. vol 11. issue 3. 2000-05-11. PMID:10786822. |
in addition, we questioned whether serotonin neurons of guinea pigs contain ovarian steroid receptors (estrogen receptoralpha[eralpha], estrogen receptor beta[erbeta], progestin receptors [prs]) that could directly mediate the actions of e or p. western blot and densitometric analysis for tph were used on raphe extracts from untreated-ovariectomized (ovx), ovx-e-treated (28 d), and ovx-e+p-treated (14 d e+14 d e+p) guinea pigs. |
2000-05-11 |
2023-08-12 |
Not clear |
| J E McDuffie, E D Motley, L E Limbird, M A Malequ. 5-hydroxytryptamine stimulates phosphorylation of p44/p42 mitogen-activated protein kinase activation in bovine aortic endothelial cell cultures. Journal of cardiovascular pharmacology. vol 35. issue 3. 2000-04-11. PMID:10710124. |
as shown by western blot analysis, 5-ht and the 5-ht1b-selective agonist 5-nonyloxytryptamine (5-not) stimulate time- and concentration-dependent (0.001-10 microm) phosphorylation of mapk in these cells. |
2000-04-11 |
2023-08-12 |
cattle |
| T Nilsson, J Longmore, D Shaw, E Pantev, J A Bard, T Branchek, L Edvinsso. Characterisation of 5-HT receptors in human coronary arteries by molecular and pharmacological techniques. European journal of pharmacology. vol 372. issue 1. 1999-07-21. PMID:10374714. |
expression of messenger ribonucleic acid (mrna) encoding different 5-ht receptors in human coronary arteries, atrium, ventricle wall and epicardium was determined using reverse transcriptase-polymerase chain reaction (rt-pcr) and southern blot analysis. |
1999-07-21 |
2023-08-12 |
human |
| B Jernej, D Hranilović, L Cicin-Sai. Serotonin transporter on rat platelets: levels of mRNA underlie inherited differences in uptake kinetics. Neurochemistry international. vol 33. issue 6. 1999-05-06. PMID:10098721. |
in order to study the genetic background of these inherited differences, comparative northern blot analysis of the platelet serotonin transporter messenger rna levels of the animals from the two sublines was performed. |
1999-05-06 |
2023-08-12 |
rat |
| J Liu, P Y Wang, D C Luo, Z H Yu, B Y Su. [Effects of serotonin on the proliferation of pulmonary arterial smooth muscle cell under hypoxia]. Sheng li xue bao : [Acta physiologica Sinica]. vol 49. issue 3. 1998-12-16. PMID:9812814. |
the effects of hypoxia on the bovine pulmonary arterial smooth muscle cell (pasm) proliferation and serotonin transporter gene expression were investigated using 3h-thymidine incorporation, immunocytochemical staining and mrna dot blot hybridyzation, the results were as follows, pasm 3h-thymidine incorporation increased in response to anoxia for 24 h (p < 0.05). |
1998-12-16 |
2023-08-12 |
cattle |
| J Liu, P Y Wang, D C Luo, Z H Yu, B Y Su. [Effects of serotonin on the proliferation of pulmonary arterial smooth muscle cell under hypoxia]. Sheng li xue bao : [Acta physiologica Sinica]. vol 49. issue 3. 1998-12-16. PMID:9812814. |
dot blot analysis showed hypoxia increased serotonin transporter mrna expression of pasm (p < 0.01). |
1998-12-16 |
2023-08-12 |
cattle |
| K Kageyama, F Tozawa, N Horiba, H Watanobe, T Sud. Serotonin stimulates corticotropin-releasing factor gene expression in the hypothalamic paraventricular nucleus of conscious rats. Neuroscience letters. vol 243. issue 1-3. 1998-05-14. PMID:9535102. |
northern blot analysis revealed that the crf messenger rna (mrna) level in the pvn as well as the proopiomelanocortin mrna level in the anterior pituitary significantly increased 120 min after the 5-ht injections (50-250 nmol/side). |
1998-05-14 |
2023-08-12 |
rat |
| K P Lesch, J Meyer, K Glatz, G Flügge, A Hinney, J Hebebrand, S M Klauck, A Poustka, F Poustka, D Bengel, R Mössner, P Riederer, A Heil. The 5-HT transporter gene-linked polymorphic region (5-HTTLPR) in evolutionary perspective: alternative biallelic variation in rhesus monkeys. Rapid communication. Journal of neural transmission (Vienna, Austria : 1996). vol 104. issue 11-12. 1998-04-29. PMID:9503271. |
pcr, southern blot, and sequence analyses of the 5-ht transporter gene's 5'-flanking region in different mammalian species confirmed the presence of the 5-httlpr in platyrrhini and catarrhini (hominoids, cercopithecoids) but not in prosimian primates and other mammals. |
1998-04-29 |
2023-08-12 |
human |
| R Franzen, J Schoenen, P Leprince, E Joosten, G Moonen, D Marti. Effects of macrophage transplantation in the injured adult rat spinal cord: a combined immunocytochemical and biochemical study. Journal of neuroscience research. vol 51. issue 3. 1998-03-31. PMID:9486767. |
at various survival times, we have studied their effect on the expression of neuronal (neurofilaments [nf], calcitonin gene-related peptide [cgrp], 5-hydroxytryptamine [5-ht]) and nonneuronal markers (myelin-associated glycoprotein [mag], glial fibrillary acidic protein [gfap], laminin) by using semiquantitative western blot and immunohistochemical techniques. |
1998-03-31 |
2023-08-12 |
rat |
| D Hranilović, K P Lesch, D Ugarković, L Cicin-Sain, B Jerne. Identification of serotonin transporter mRNA in rat platelets. Journal of neural transmission (Vienna, Austria : 1996). vol 103. issue 8-9. 1997-05-12. PMID:9013389. |
northern blot analysis, using 32p-labeled 5htt cdna as a probe, identified approximately 3.3 kb long 5htt mrna. |
1997-05-12 |
2023-08-12 |
rat |
| C Gargalidis-Moudanos, A Remaury, N Pizzinat, A Parin. Predominant expression of monoamine oxidase B isoform in rabbit renal proximal tubule: regulation by I2 imidazoline ligands in intact cells. Molecular pharmacology. vol 51. issue 4. 1997-05-06. PMID:9106629. |
two findings indicate that mao-b is the predominant isoform expressed in the renal proximal tubule cells: 1) western blot performed with an anti-mao-a/mao-b polyclonal antiserum revealed a single 55-kda band corresponding to mao-b; 2) enzyme assays showed an elevated mao-b activity ([14c]beta-phenylethylamine oxidation: vmax = 1.31 +/- 0.41 nmol/min/mg protein), whereas mao-a activity was only detectable ([14c]5-ht oxidation: vmax = 80.3 +/- 19 pmol/min/mg protein). |
1997-05-06 |
2023-08-12 |
rabbit |
| R Kekuda, V Torres-Zamorano, F H Leibach, V Ganapath. Human serotonin transporter: regulation by the neuroprotective agent aurintricarboxylic acid and by epidermal growth factor. Journal of neurochemistry. vol 68. issue 4. 1997-04-28. PMID:9084414. |
northern blot hybridization revealed that ata treatment caused a marked increase in the steady-state levels of serotonin transporter-specific transcripts. |
1997-04-28 |
2023-08-12 |
human |
| S Ovalle, E Casanova, C Garate, A Alonso-Llamazares, M A Chinchetru, P Calv. Immunodetection of serotonin transporter from mouse brain. Neuroreport. vol 6. issue 17. 1996-09-24. PMID:8747152. |
western blot analysis of mouse brain membranes detected the serotonin transporter as a 71 kda polypeptide. |
1996-09-24 |
2023-08-12 |
mouse |
| S W Watts, C H Yeum, G Campbell, R C Web. Serotonin stimulates protein tyrosyl phosphorylation and vascular contraction via tyrosine kinase. Journal of vascular research. vol 33. issue 4. 1996-08-30. PMID:8695753. |
western blot analysis using antiphosphotyrosine antibody confirmed that 5-ht produced a time- and concentration-dependent increase in the phosphotyrosine immunoreactivity of a 42-kd protein in cultured aortic smooth muscle cells. |
1996-08-30 |
2023-08-12 |
rat |