| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Wei-Zhe Liang, Yuan-Yi Chia, Huai-Jhih Sun, Gwo-Ching Su. Exploration of beauvericin's toxic effects and mechanisms in human astrocytes and N-acetylcysteine's protective role. Toxicon : official journal of the International Society on Toxinology. 2024-04-26. PMID:38670497. |
western blot analysis of cells treated with bea revealed altered protein levels of bax, cleaved caspase-9, and caspase-3, along with an increased bax/bcl-2 ratio, indicating the induction of apoptosis. |
2024-04-26 |
2024-04-29 |
human |
| Jiuming Dai, Jin Liu, Yucheng Shen, Bing Zhang, Chaonian Li, Zhidong Li. Regulation of endoplasmic reticulum stress on autophagy and apoptosis of nucleus pulposus cells in intervertebral disc degeneration and its related mechanisms. PeerJ. vol 12. 2024-04-26. PMID:38666076. |
cell apoptosis was detected by flow cytometry and the expression of lc3ii/i was detected by western blot. |
2024-04-26 |
2024-04-28 |
Not clear |
| Jiuming Dai, Jin Liu, Yucheng Shen, Bing Zhang, Chaonian Li, Zhidong Li. Regulation of endoplasmic reticulum stress on autophagy and apoptosis of nucleus pulposus cells in intervertebral disc degeneration and its related mechanisms. PeerJ. vol 12. 2024-04-26. PMID:38666076. |
then, above functional experiments were conducted again and western blot was employed for the evaluation of autophagy-, apoptosis and ers-related proteins. |
2024-04-26 |
2024-04-28 |
Not clear |
| Sheng-Yun Wu, Ke-Ru Cheng, Yan-Yun Zhou, Yin-Fang Wan. [Microtubule-associated tumor suppressor 1 inhibits hemin-induced apoptosis of vascular endothelial cells via hemeoxygenase 1]. Sheng li xue bao : [Acta physiologica Sinica]. vol 76. issue 2. 2024-04-25. PMID:38658371. |
the effect of mtus1 or hmox1 knockdown on hemin-induced apoptosis in huvec, and the overexpression of nrf2 on hemin-induced apoptosis in mtus1 knockdown 293t cells were assayed with cck8 and western blot. |
2024-04-25 |
2024-04-28 |
human |
| Yunhao Jia, Han Yang, Jinsong Yu, Zhong Li, Guangwei Jia, Bo Din. Crocin enhances the sensitivity to paclitaxel in human breast cancer cells by reducing BIRC5 expression. Chemical biology & drug design. vol 103. issue 2. 2024-04-25. PMID:38661582. |
baculoviral inhibitor of apoptosis repeat containing 5 (birc5) expression was detected by qrt-pcr and western blot analysis. |
2024-04-25 |
2024-04-28 |
human |
| Zhiguang Liu, Chuang Sun, Yujia Shi, Zhipeng Wang, Lianzheng Zhou, Qian Zhang, Zhengdao Ma. MiR-4454 in Combined Allergic Rhinitis and Asthma Syndrome (CARAS): Clinical significance and mechanistic insights into airway inflammation. Cellular and molecular biology (Noisy-le-Grand, France). vol 70. issue 3. 2024-04-23. PMID:38650129. |
house dust mite (hdm) sensitization and challenge-induced bronchial epithelial cells to simulate the asthma state model in vitro, mir-4454 mimics and inhibitor transfection to detect the expression level of pro-inflammatory cytokines, cell survival rate and migration ability, flow cytometry and western blot (wb) detection of cell cycle, apoptosis and inflammation-related protein levels. |
2024-04-23 |
2024-04-26 |
human |
| Kurnegala Manikanta, Manoj Paul, Vaddaragudisalu D Sandesha, Shanmuga S Mahalingam, Thimmasandra Narayan Ramesh, Krishnegowda Harishkumar, Shashank S Koundinya, Shivanna Naveen, Kempaiah Kemparaju, Kesturu S Giris. Oxidative Stress-Induced Platelet Apoptosis/Activation: Alleviation by Purified Curcumin via ASK1-JNK/p-38 Pathway. Biochemistry. Biokhimiia. vol 89. issue 3. 2024-04-22. PMID:38648762. |
western blot analysis confirms protective effect of the purified curcumin against oxidative stress-induced platelet apoptosis and activation via downregulation of mapks protein activation, including ask1, jnk, and p-38. |
2024-04-22 |
2024-04-26 |
Not clear |
| Hongjuan Duan, Shaojin Li, Chuangwei Li, Lan Tian, Yun Ma, Xiaoyan Ca. Alfalfa xeno-miR159a regulates bovine mammary epithelial cell proliferation and milk protein synthesis by targeting PTPRF. Scientific reports. vol 14. issue 1. 2024-04-20. PMID:38643232. |
cell proliferation and apoptosis were detected using the cell counting kit 8 (cck-8) assay, 5-ethynyl-2'-deoxyuridine (edu) staining, western blot, and flow cytometry. |
2024-04-20 |
2024-04-23 |
cattle |
| Fatma Uysal, Gozde Sukur, Nazlican Bozdemir, Ozgur Cina. Antioxidant supplementation may effect DNA methylation patterns, apoptosis, and ROS levels in developing mouse embryos. Histochemistry and cell biology. 2024-04-16. PMID:38627305. |
protein expression levels of dnmt1, 3a, 3b, and 3l enzymes were analyzed by immunofluorescence and western blot, while global dna methylation, apoptosis, and ros levels were evaluated by immunofluorescence. |
2024-04-16 |
2024-04-19 |
mouse |
| Yining Lei, Manqin Li, Xinran Liu, Lu Zhang, Ruyi Zhang, Fei Ca. Nerolidol rescues hippocampal injury of diabetic rats through inhibiting NLRP3 inflammasome and regulation of MAPK/AKT pathway. BioFactors (Oxford, England). 2024-04-16. PMID:38624190. |
western blot was used to detect apoptosis (bcl-2, bax, and cleaved-caspase-3); synapses (postsynaptic densifying protein 95, syn1, and synaptophysin); mitochondria (drp1, opa1, mfn1, and mfn2); er (grp78, atf6, chop, and caspase-12); nlrp3 inflammasome (nlrp3, asc, and caspase-1); inflammatory cytokines (il-18, il-1β, and tnf-α); akt (p-akt); and mitogen-activated protein kinase (mapk) pathway (p-erk, p-p38, and p-jnk) related protein expression. |
2024-04-16 |
2024-04-18 |
rat |
| Ye-Won Kim, Seul-Gi Yang, Byoung-Boo Seo, Deog-Bon Koo, Hyo-Jin Par. Deoxynivalenol leads to endoplasmic reticulum stress-mediated apoptosis via the IRE1/JNK/CHOP pathways in porcine embryos. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2024-04-12. PMID:38608924. |
western blot analysis showed that ire1/jnk/chop signaling was activated in don-exposed porcine embryos, indicating that er stress-associated apoptosis was instigated. |
2024-04-12 |
2024-04-15 |
Not clear |
| Showkat Ali Ganie, Rayees Ahmad Naik, Ovas Ahmad Dar, Luqman Jameel Rather, Mohammed A Assiri, Qing L. Design and fabrication of functionalized curdlan-curcumin delivery system to facilitate the therapeutic effects of curcumin on breast cancer. International journal of biological macromolecules. 2024-04-12. PMID:38608982. |
the results obtained from western blot analysis demonstrated that the co-administration of cur and cum effectively induced apoptosis in mcf-7 cells. |
2024-04-12 |
2024-04-15 |
Not clear |
| Xiaomeng Xu, Cheng Yin, Bing Dong, Yuewen Li, Shi Liu, Jun Che. LncRNA XIST Protects Against Polycystic Ovary Syndrome via the Regulation of miR-212-3p/RASA1 Axis. Biochemical genetics. 2024-04-12. PMID:38609670. |
cell viability, apoptosis, protein expression of bcl-2 and bax were assessed by mtt, flow cytometry analysis, rt-qpcr and western blot, respectively. |
2024-04-12 |
2024-04-15 |
Not clear |
| Mingbao Gu, Chenchao Li, Qingyu Deng, Ximiao Chen, Rui Le. Celastrol enhances the viability of random-pattern skin flaps by regulating autophagy through the AMPK-mTOR-TFEB axis. Phytotherapy research : PTR. 2024-04-11. PMID:38600729. |
the effects of cel on flap angiogenesis, apoptosis, oxidative stress, and autophagy were evaluated by western blot, immunohistochemistry, and immunofluorescence assays. |
2024-04-11 |
2024-04-13 |
Not clear |
| Xunxia Zhu, Xiaoyu Chen, Xuelin Zhang, Liting Zhao, Xiaoyong She. MicroRNA‑373‑3p inhibits the proliferation and invasion of non‑small‑cell lung cancer cells by targeting the GAB2/PI3K/AKT pathway. Oncology letters. vol 27. issue 5. 2024-04-08. PMID:38586211. |
to further examine how mir-373 mediates the emergence of nsclc, western blot, cell counting kit-8, cell invasion and wound-healing assays, as well as apoptosis detection and a luciferase assay were used. |
2024-04-08 |
2024-04-10 |
Not clear |
| Zhitong Lin, Chen Liu, Ziqin Yan, Jing Cheng, Xiancheng Wang, Feilong Zhou, Xilin Lyu, Shiyan Zhang, Daizhou Zhang, Xiangjing Meng, Yujun Zha. Synthesis and biological evaluation of 4-imidazolidinone-containing compounds as potent inhibitors of the MDM2/p53 interaction. European journal of medicinal chemistry. vol 270. 2024-04-06. PMID:38581730. |
mechanistic studies show that 2 and 22 dose-dependently activated p53 and its target genes and induced apoptosis in cells based on the western blot, qpcr, and flow cytometry assays. |
2024-04-06 |
2024-04-10 |
Not clear |
| Daxu Liu, Qing Zhao, Zhaoxu Tu, Siyu Zhang, Shuo Deng, Zhiqiang Xiong, Jin Zeng, Fengchang Wu, Xuejiao Zhang, Baoshan Xin. Inhibitory effects of black phosphorus nanosheets on tumor cell proliferation through downregulation of ADIPOQ and downstream signaling pathways. Chemico-biological interactions. 2024-04-06. PMID:38582339. |
herein, we employed a range of biological techniques, including cytotoxicity measurement, bioinformatics tools, proteomics, target gene overexpression, western blot analysis, and apoptosis detection, to investigate the toxicity of bpnss across a549, hepg-2, mcf-7, and caco-2 cell lines. |
2024-04-06 |
2024-04-10 |
Not clear |
| Yuanlin Wu, Jiandong Zhang, Junjun Zhao, Bin Wan. Folate-modified liposomes mediate the co-delivery of cisplatin with miR-219a-5p for the targeted treatment of cisplatin-resistant lung cancer. BMC pulmonary medicine. vol 24. issue 1. 2024-04-02. PMID:38561695. |
cellular functional experiments, apoptosis assays, and western blot demonstrated that lipo@ddp@mir-219a-5p@fa effectively reversed ddp resistance in a549 cells, inhibited cell proliferation and migration, and further promoted apoptosis. |
2024-04-02 |
2024-04-04 |
human |
| Yamei Wu, Jing Zhao, Can Cui, Yao Zhang, Qing Zhu, Shunshun Han, Chaowu Yang, Huadong Yi. MiRNA-21-5p induces chicken hepatic lipogenesis by targeting NFIB and KLF3 to suppress the PI3K/AKT signaling pathway. Journal of animal science. 2024-04-02. PMID:38563227. |
then the qpcr, western blot, flow cytometry, elisa, dual-luciferase, and immunofluorescence assays were respectively used to determine the impact of mir-21-5p in the chicken liver, and it turned out that mir-21-5p enhanced lipogenesis, oxidative stress, and inflammatory responses, which ultimately induced hepatocyte apoptosis. |
2024-04-02 |
2024-04-04 |
chicken |
| Huang Linhuan, Zhong Liangying, Lin Shaobin, Zhu Caixia, Cai Danlei, Huang Siqi, Hong Peiming, Kong Shu, Xie Yingjun, Luo Yanmi. Effect of MSX1 on the cellular function of cardiomyocytes. Gene. 2024-03-31. PMID:38556116. |
in this study, we used ac16 cell culture, real-time fluorescence quantitative pcr (qpcr), protein blotting (western blot), flow cytometry apoptosis assay and lactate dehydrogenase (ldh) elisa (enzyme-linked immunosorbnent assay) to investigate the effect of the msx1 gene on cardiomyocyte function. |
2024-03-31 |
2024-04-03 |
Not clear |