| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Jiajiong Yang, Hong Wu, Nan Hu, Xiaosong Gu, Fei Din. Effects of bone marrow stromal cell-conditioned medium on primary cultures of peripheral nerve tissues and cells. Neurochemical research. vol 34. issue 9. 2009-10-19. PMID:19350389. |
in this study, rat dorsal root ganglion (drg) explants, rat drg neurons, and rat schwann cells (scs) were treated with monkey msc-conditioned medium, respectively, and then subjected to mtt assay, bromodeoxyuridine/hoechst 33342 double staining, flow cytometry, immunohistochemistry, real-time quantitative pcr, and western blot analysis, respectively. |
2009-10-19 |
2023-08-12 |
rat |
| C Chen, P Tang, J Yue, P Ren, X Liu, X Zhao, Z Y. Effect of siRNA targeting HIF-1alpha combined L-ascorbate on biological behavior of hypoxic MiaPaCa2 cells. Technology in cancer research & treatment. vol 8. issue 3. 2009-08-11. PMID:19445542. |
then, we observed the expression of hif-1alpha mrna and protein, the proliferation, apoptosis, and migration of miapaca2 cells by real-time pcr, western blot, mtt, tunel, and transwell assay, respectively. |
2009-08-11 |
2023-08-12 |
human |
| Wen-Juan Wang, Qing-Quan Li, Jing-Da Xu, Xi-Xi Cao, Hai-Xia Li, Feng Tang, Qi Chen, Jin-Ming Yang, Zu-De Xu, Xiu-Ping Li. Over-expression of ubiquitin carboxy terminal hydrolase-L1 induces apoptosis in breast cancer cells. International journal of oncology. vol 33. issue 5. 2008-12-16. PMID:18949367. |
using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (mtt) assay, western blot, hoechst 33258 staining assay and flow cytometry, we found that over-expression of uch-l1 in mcf7 cells induced apoptosis. |
2008-12-16 |
2023-08-12 |
mouse |
| Wei Zhou, Xu Zhu, Liang Zhu, Yong Yao Cui, Hao Wang, Hong Qi, Qiu Shi Ren, Hong Zhuan Che. Neuroprotection of muscarinic receptor agonist pilocarpine against glutamate-induced apoptosis in retinal neurons. Cellular and molecular neurobiology. vol 28. issue 2. 2008-07-30. PMID:18172757. |
in rat primary retinal cultures, identification of neuron, cell viability, apoptosis, intracellular ca(2+) concentration, mitochondrial membrane potential, gene expression were studied by immunofluorescence, mtt, high content scanning, confocal microscopy, reverse-transcription pcr, and western blot analysis, respectively. |
2008-07-30 |
2023-08-12 |
rat |
| Jae Hong Seo, Hwa Jung Sung, Chul Won Choi, Byung Soo Kim, Sang Won Shin, Yeul Hong Kim, Bon Hong Min, Jun Suk Ki. Extrinsic nitric oxide donor partially reverses arginine deiminase induced cell growth inhibition through NFkappaB and Bcl-X L. Investigational new drugs. vol 26. issue 3. 2008-07-28. PMID:18180877. |
an mtt assay was used to assess cell survival after treatment, nitric oxide assays to determine nitrite levels and western blot analysis to determine the expression of the no mediators, nfkappab and bcl-x l. interestingly, we found that the extrinsic no donor only partially reversed adi-induced inhibition of cell growth in a dose-dependent pattern and resulted in an induction of nfkappab and bcl-x l expression. |
2008-07-28 |
2023-08-12 |
human |
| Sanjica Jakopec, Dalibor Karlović, Klara Dubravcić, Drago Batinić, Jasna Sorić, Anamaria Brozović, Danijel Buljan, Maja Osma. Lithium effect on glutamate induced damage in glioblastoma cells. Collegium antropologicum. vol 32 Suppl 1. 2008-05-01. PMID:18405064. |
cytotoxicity of glutamate was examined by spectrophotometric mtt assay, while the expression of apoptosis related genes was examined by western blot method. |
2008-05-01 |
2023-08-12 |
human |
| Qing Wu, Yan Chen, Xing-Gang Li, Yuan-Yan Tan. [Regulatory effect of curcumin on p300 and HDAC1 in B-NHL cells]. Zhongguo shi yan xue ye xue za zhi. vol 14. issue 2. 2007-12-14. PMID:16638200. |
the in vitro cultured raji cells were treated with curcumin at various concentrations (6.25-50 micromol/l) and at different time points (0, 6, 12, 24 and 48 hours), the inhibitory ratio of cell growth was measured by mtt assay, the cell apoptosis rate was detected by flow cytometry with annexin v-fitc/pi double staining, the changes of p300 and hdac1 mrna expression and protein level in raji cells were determined by rt-pcr and western blot. |
2007-12-14 |
2023-08-12 |
Not clear |
| b' J Mal\\xc3\\xadkov\\xc3\\xa1, A Zdarilov\\xc3\\xa1, A Hlobilkov\\xc3\\xa1, J Ulrichov\\xc3\\xa. The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine. Cell biology and toxicology. vol 22. issue 6. 2007-04-12. PMID:16964588.' |
che and sa treatment of cell lines for 24 h resulted in (1) inhibition of cell viability in a dose-dependent manner in all tested cells (as evaluated by mtt test and bromodeoxyuridine incorporation assay); (2) dose-dependent increase in dna damage in all tested cells (as evaluated by dna comet assay); (3) changes in apoptosis (assessed by western blot analysis and tunel assay); and (4) significant induction of cyclin kinase inhibitors p21(waf1/cip1) and p27(kip1) in prostate cancer cells (identified by western blot analysis). |
2007-04-12 |
2023-08-12 |
human |
| Sandra Osbild, Laurent Brault, Eric Battaglia, Denyse Bagre. Resistance to cisplatin and adriamycin is associated with the inhibition of glutathione efflux in MCF-7-derived cells. Anticancer research. vol 26. issue 5A. 2006-11-30. PMID:17094488. |
cytotoxicity was evaluated by mtt assay, reactive oxygen species (ros), apoptosis and necrosis by flow cytometry, glutathione (gsh) by hplc, and bcl-2, bax and parp expression by western blot. |
2006-11-30 |
2023-08-12 |
human |
| Chris A Owen, Jamuna Selvakumaran, Ioan Notingher, Gavin Jell, Larry L Hench, Molly M Steven. In vitro toxicology evaluation of pharmaceuticals using Raman micro-spectroscopy. Journal of cellular biochemistry. vol 99. issue 1. 2006-11-06. PMID:16598770. |
dna fragmentation was confirmed by western blot analysis of apoptosis regulator protein p53 and cell metabolic activity determined by mtt assay. |
2006-11-06 |
2023-08-12 |
human |
| Zhen Xian Du, Hai Yan Zhang, Da Xin Gao, Hua Qin Wang, Yong Jun Li, Guo Liang Li. Antisurvivin oligonucleotides inhibit growth and induce apoptosis in human medullary thyroid carcinoma cells. Experimental & molecular medicine. vol 38. issue 3. 2006-08-18. PMID:16819281. |
in tt cells, we confirmed survivin expression and its down-regulation by asodns using rt-pcr and western blot analyses, and investigated effects of asodns on viability and growth by mtt assay and apoptosis by apoptotic analyses including dna laddering assay, acridine orange/ethidium bromide staining and flow cytometric cell cycle analysis. |
2006-08-18 |
2023-08-12 |
human |
| Jiajun Liu, Renwei Huang, Dongjun Lin, Xiangyuan Wu, Jun Peng, Qu Lin, Xianglin Pan, Maohong Zhang, Ming Hou, Feng Che. Apoptotic effect of oridonin on NB4 cells and its mechanism. Leukemia & lymphoma. vol 46. issue 4. 2005-10-05. PMID:16019488. |
cell growth inhibition, apoptosis and related pathways were assessed by mtt assay as well as flow cytometry (fcm) and western blot analysis. |
2005-10-05 |
2023-08-12 |
Not clear |
| Hyun-Kyung Chang, Hye-Young Yang, Taeck-Hyun Lee, Min-Chul Shin, Myoung-Hwa Lee, Mal-Soon Shin, Chang-Ju Kim, Ok-Jin Kim, Seon-Pyo Hong, Sonhae Ch. Armeniacae semen extract suppresses lipopolysaccharide-induced expressions of cyclooxygenase [correction of cycloosygenase]-2 and inducible nitric oxide synthase in mouse BV2 microglial cells. Biological & pharmaceutical bulletin. vol 28. issue 3. 2005-09-13. PMID:15744067. |
in this study, the effect of armeniacae semen extract on lipopolysaccharide-induced inflammation was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, reverse transcription-polymerase chain reaction (rt-pcr), western blot, prostaglandin e2 immunoassay, and nitric oxide detection on mouse bv2 microglial cells. |
2005-09-13 |
2023-08-12 |
mouse |
| Myoung-Hwa Lee, Mi-Hyeon Jang, Eun-Kyung Kim, Sang-Wan Han, Seong-Yeon Cho, Chang-Ju Ki. Nitric oxide induces apoptosis in mouse C2C12 myoblast cells. Journal of pharmacological sciences. vol 97. issue 3. 2005-08-29. PMID:15781989. |
to investigate whether nitric oxide (no) induces apoptosis in myoblast cells, the effect of the sodium nitroprusside (snp), no donor, on the apoptosis of mouse c2c12 myoblast cells was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, flow cytometry, 4,6-diamidino-2-phenylindole (dapi) staining, terminal deoxynucleotidyl transferase (tdt)-mediated dutp nick end labeling (tunel) assay, dna fragmentation assay, reverse transcription-polymerase chain reaction (rt-pcr), western blot analysis, and caspase-3 enzyme assay. |
2005-08-29 |
2023-08-12 |
mouse |
| Stephen Hsu, Kajuana Farrey, John Wataha, Jill Lewis, James Borke, Baldev Singh, Haiyan Qin, Carol Lapp, David Lapp, Tuan Nguyen, George Schuste. Role of p21WAF1 in green tea polyphenol-induced growth arrest and apoptosis of oral carcinoma cells. Anticancer research. vol 25. issue 1A. 2005-05-16. PMID:15816520. |
the current study determined the impact of sirna-suppressed p21waf1 and its response to egcg on cell growth, dna synthesis and apoptosis by rt-pcr, western blot, brdu incorporation, mtt and caspase 3 activity assays. |
2005-05-16 |
2023-08-12 |
Not clear |
| Jin-Woo Park, Mariwil G Wong, Margaret Lobo, William C Hyun, Quan-Yang Duh, Orlo H Clar. Modulation of tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by chemotherapy in thyroid cancer cell lines. Thyroid : official journal of the American Thyroid Association. vol 13. issue 12. 2004-08-04. PMID:14751030. |
we used flow cytometry to measure apoptosis, dimethyl-thiazol-diphenyltetrazolium bromide (mtt) assay to measure antiproliferation effects and western blot to determine the expression of bcl family proteins. |
2004-08-04 |
2023-08-12 |
human |
| J H Yang, F Feng, H Qian, H Chen. Chemosensitization of breast carcinoma cells with the use of bcl-2 antisense oligodeoxynucleotide. Breast (Edinburgh, Scotland). vol 13. issue 3. 2004-07-30. PMID:15177426. |
changes in expression of the bcl-2 gene were examined by western blot; cell growths were detected by mtt assay, and apoptosis rates were detected by flow cytometry (fcm). |
2004-07-30 |
2023-08-12 |
Not clear |
| F Marinelli, D La Sala, G Cicciotti, L Cattini, C Trimarchi, S Putti, A Zamparelli, L Giuliani, G Tomassetti, Caterina Cint. Exposure to 900 MHz electromagnetic field induces an unbalance between pro-apoptotic and pro-survival signals in T-lymphoblastoid leukemia CCRF-CEM cells. Journal of cellular physiology. vol 198. issue 2. 2004-02-06. PMID:14603534. |
we evaluated the effects of high-frequency emf on cell growth rate and apoptosis induction, by cell viability (mtt) test, facs analysis and dna ladder, and we investigated pro-apoptotic and pro-survival signaling pathways possibly involved as a function of exposure time by western blot analysis. |
2004-02-06 |
2023-08-12 |
Not clear |
| Jian Li, Lu Zuo, Ti Shen, Zhi-Nan Zhan. [Inhibition of the activation of transcriptional factor NFkappaB during sodium selenite-induced apoptosis in NB4 cells]. Zhongguo shi yan xue ye xue za zhi. vol 10. issue 5. 2003-08-12. PMID:12513738. |
in order to evaluate the effect of sodium selenite on the activation of nfkappab during selenite-induced apoptosis in nb4 cells, western blot was used to measure the level of p65 in nuclear extraction of nb4 cells treated with sodium selenite to reflect the activation of nfkappab; the apoptosis of nb4 cells was determined by morphological observation, dna ladder electrophoresis and flow cytometry; and mtt test was used to measure the growth inhibition of cells. |
2003-08-12 |
2023-08-12 |
Not clear |
| Mi-Hyeon Jang, Min-Chul Shin, Hye-Sook Shin, Khae-Hawn Kim, Heung-Jae Park, Ee-Hwa Kim, Chang-Ju Ki. Alcohol induces apoptosis in TM3 mouse Leydig cells via bax-dependent caspase-3 activation. European journal of pharmacology. vol 449. issue 1-2. 2003-03-20. PMID:12163104. |
to investigate whether ethanol induces apoptosis in leydig cells, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, terminal deoxynuclotidyl transferase-mediated dutp nick end-labeling (tunel) assay, 4,6-diamidino-2-phenylindole (dapi) staining, dna fragmentation assay, caspase-3 enzyme assay, reverse transcription-polymerase chain reaction (rt-pcr) and western blot analysis were performed on tm3 mouse leydig cells. |
2003-03-20 |
2023-08-12 |
mouse |