| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Xiao-Xia Wang, Xiao-Qin Liu, Wei Zhang, Xuan Chen, Li Zha. [Orphanin FQ combined with adriamycin reverses multi-drug resistance of K562/ADM and its molecular mechanism]. Zhongguo shi yan xue ye xue za zhi. vol 20. issue 3. 2013-05-16. PMID:22739158. |
mtt method was used to detect the proliferation ability of k562/adm treated with ofq and adm alone and their combination; flow cytometry was performed to measure the cell apoptosis rate; real time-pcr was applied to detect the mdr1 mran expression; western blot was used to determine the p-gp expression. |
2013-05-16 |
2023-08-12 |
Not clear |
| Wei Tan, Wei Zhou, Hong-gang Yu, He-Sheng Luo, Lei She. The DNA methyltransferase inhibitor zebularine induces mitochondria-mediated apoptosis in gastric cancer cells in vitro and in vivo. Biochemical and biophysical research communications. vol 430. issue 1. 2013-05-14. PMID:23167995. |
in the study, the effects of zebularine on the growth and apoptosis of gastric cancer cells were investigated by mtt assay, hoechst assay, western blot analysis, flow cytometric analysis of annexin v-fitc/pi staining, and tunel assay. |
2013-05-14 |
2023-08-12 |
mouse |
| Cheng Chen, Ji-Shi Wang, Chang Ynag, Yan-Yan Yu, Yan Li, Dan Ma, Qin Fan. [CML cell apoptosis induced by AMN107 combined with heme oxygenase-1 inhibitor]. Zhongguo shi yan xue ye xue za zhi. vol 20. issue 4. 2013-05-09. PMID:22931644. |
proliferative rate of cells treated with amn107 (10 µmol/l) and znppix (10 µmol/l) alone or both for different time was observed by mtt and trypan blue methods; the expression of ho-1 in the control group, znppix (10 µmol/l) group, amn107 (10 µmol/l) group, amn107 (10 µmol/l) combined with znppix (10 µmol/l) group was evaluated by semi-quantitative rt-pcr and western blot at 48 h. cell apoptosis was detected by flow cytometry with annexin v/pi double staining at 48 h. the results showed that the strongest inhibition of cell proliferation was detected in combined group, and in a time-dependent manner; the expression level of ho-1 was lowest in combined group; the cell apoptosis rates were (11.38 ± 0.02)%, (17.44 ± 0.08)%, (39.81 ± 0.07)% and (56.46 ± 0.19)% in the control group, znppix group, amn107 group, amn107 combined with znppix group at 48 h respectively. |
2013-05-09 |
2023-08-12 |
Not clear |
| Gonghua Hu, Haiyan Huang, Lingqing Yang, Caigao Zhong, Bo Xia, Yuan Yang, Jianjun Liu, Desheng Wu, Qingchen Liu, Zhixiong Zhuan. Down-regulation of Polη expression leads to increased DNA damage, apoptosis and enhanced S phase arrest in L-02 cells exposed to hydroquinone. Toxicology letters. vol 214. issue 2. 2013-03-18. PMID:22981619. |
in this study, cell survival rate was determined using the mtt assay, dna damage was determined by the comet assay, apoptosis and cell cycle distribution were determined using flow cytometry, the mrna expression levels of polη were determined by real-time pcr, and the protein expression levels of polη and γ-h2ax were determined by western blot, γ-h2ax foci were visualized by confocal laser scanning fluorescence microscopy after cells were exposed to hq at various concentrations for 24h in vitro. |
2013-03-18 |
2023-08-12 |
Not clear |
| Jia Xie, Mei Zhang, Yan-Ping Song, Kai Hu, Jing-Jing Ren, Yun-Jie Zhan. [Caspase-independent apoptosis induced by arsenic trioxide in human multiple myeloma cell RPMI8226]. Zhongguo shi yan xue ye xue za zhi. vol 20. issue 1. 2012-12-20. PMID:22391177. |
mtt method was used to analyze the proliferation inhibition rate; flow cytometry was used to detect the apoptosis rate; western blot was used to determine the expressions of bcl-2 and caspase-3 in rpmi8226 cells. |
2012-12-20 |
2023-08-12 |
human |
| Hui-Ling Shen, Li-Li Fang, Chen Chen, Xin-Jian Fang, Qiao-Yun Chen, Juan Li, Wen-Lin X. [VEGF shRNA enhances the sensitivity of multidrug-resistant leukemia cells to anticancer agent]. Zhongguo shi yan xue ye xue za zhi. vol 19. issue 1. 2012-12-17. PMID:21362217. |
rt-pcr was used to detect the expression of vegf and mrp1 at the mrna level;western blot was used to analyze the expression of vegf, mrp1, akt, p-akt at the protein level; mtt was used to determine the ic(50) value of transfected cells to doxorubicin (dox); flow cytometry was used to detect cell apoptosis and intracellular rho123 retention. |
2012-12-17 |
2023-08-12 |
human |
| Seung Hyun Oh, Young Mi Whang, Hye-Young Min, Seung Ho Han, Ju-Hee Kang, Ki-Hoon Song, Bonnie S Glisson, Yeul Hong Kim, Ho-Young Le. Histone deacetylase inhibitors enhance the apoptotic activity of insulin-like growth factor binding protein-3 by blocking PKC-induced IGFBP-3 degradation. International journal of cancer. vol 131. issue 10. 2012-12-10. PMID:22362554. |
the effects of the combination of rigfbp-3 and a number of hdac inhibitors on cell proliferation and apoptosis were assessed in vitro and in vivo by using the mtt assay, a flow cytometry-based tunel assay, western blot analyses and the nsclc xenograft tumor model. |
2012-12-10 |
2023-08-12 |
Not clear |
| Yan-Ling Ren, Hong-Yan Ton. [Effect of homoharringtonine combined with AG490 on JAK2-STAT5 associated signal pathway in HEL cells]. Zhongguo shi yan xue ye xue za zhi. vol 19. issue 5. 2012-12-06. PMID:22040954. |
the cell survival rates were tested by mtt, apoptosis was tested by flow cytometry after hel cells were treated by 20 ng/ml hht, 100 µmol/l ag490 and 20 ng/ml hht in combination with 100 µmol/l ag490, while the signal proteins such as p-jak2, p-stat5 and bcl-xl activated by abnormal activated jak2 were tested by western blot. |
2012-12-06 |
2023-08-12 |
Not clear |
| Xu-Chun He, Jian-Da H. [Effects of emodin on human erythroleukemia cell line HEL]. Zhongguo shi yan xue ye xue za zhi. vol 19. issue 5. 2012-12-06. PMID:22040955. |
the proliferation inhibition was detected by mtt method; the change of morphology was observed by ao/eb stains; the cell cycle and apoptosis was analyzed by flow cytometry; the expressions of akt, p-akt, p-gsk3β and hsp70 proteins were determined by western blot. |
2012-12-06 |
2023-08-12 |
human |
| Suguru Dateoka, Yuichi Ohnishi, Kenji Kakud. Effects of CRM197, a specific inhibitor of HB-EGF, in oral cancer. Medical molecular morphology. vol 45. issue 2. 2012-10-30. PMID:22718294. |
cells treated with crm197 were analyzed based on cell viability, mtt assay, invasion assay, western blot, and zymography. |
2012-10-30 |
2023-08-12 |
mouse |
| Jie Cao, Dong-Ling Dai, Long Yao, Hui-Hong Yu, Bo Ning, Qin Zhang, Juan Chen, Wen-Hui Cheng, Wei Shen, Zhao-Xia Yan. Saturated fatty acid induction of endoplasmic reticulum stress and apoptosis in human liver cells via the PERK/ATF4/CHOP signaling pathway. Molecular and cellular biochemistry. vol 364. issue 1-2. 2012-09-20. PMID:22246806. |
mtt assay was used for cell viability, flow cytometry and hoechst 33258 staining for apoptosis, rt-pcr for mrna expression, and western blot for protein expression. |
2012-09-20 |
2023-08-12 |
human |
| Zu-An Zhu, Zheng-Qiu Zhu, Hong-Xing Cai, Ying Li. Reversion of multidrug resistance by SKI-II in SGC7901/DDP cells and exploration of underlying mechanisms. Asian Pacific journal of cancer prevention : APJCP. vol 13. issue 2. 2012-09-20. PMID:22524836. |
then cell growth, apoptosis, micro- morphological changes, and expression of sphk1, p-gp, nf-kb, bcl-2 and bax were assessed by mtt assay, flow cytometry, electron microscopy, immunocytochemistry and western blot assay respectively. |
2012-09-20 |
2023-08-12 |
Not clear |
| Na Wang, Zhihua Li, Fen Tian, Ying Feng, Jintao Huang, Chaohong Li, Fukang Xi. PKCα inhibited apoptosis by decreasing the activity of JNK in MCF-7/ADR cells. Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie. vol 64. issue 5. 2012-08-31. PMID:21106355. |
mtt, dna ladder and flow cytometry were used to detect cell growth inhibition or apoptosis while western blot was used to detect the activation of proteins. |
2012-08-31 |
2023-08-12 |
Not clear |
| Ting-Tsz Ou, Chau-Jong Wang, Guang-Uei Hung, Cheng-Hsun Wu, Huei-Jane Le. Aqueous Extract of Shi-Liu-Wei-Liu-Qi-Yin Induces G2/M Phase Arrest and Apoptosis in Human Bladder Carcinoma Cells via Fas and Mitochondrial Pathway. Evidence-based complementary and alternative medicine : eCAM. vol 2011. 2012-08-23. PMID:19383839. |
we evaluated the effects of slwlqy on apoptosis-related experiments inducing in tsgh-8301 cells by (i) 3-(4,5-dimethylthiazol-zyl)-2,5-diphenylterazolium bromide (mtt) for cytotoxicity; (ii) cell-cycle analysis and (iii) western blot analysis of the g2/m-phase and apoptosis regulatory proteins. |
2012-08-23 |
2023-08-12 |
mouse |
| Fang Zeng, Yun Cheng Li, Gang Chen, Yong Kui Zhang, Ye Kai Wang, Shi Quan Zhou, Li Na Ma, Ji Hang Zhou, Yan Yan Huang, Wang Yu Zhu, Xiao Guang Li. Nicotine inhibits cisplatin-induced apoptosis in NCI-H446 cells. Medical oncology (Northwood, London, England). vol 29. issue 1. 2012-08-23. PMID:21267677. |
an mtt assay, annexin v-fitc staining, rt-pcr, and western blot were applied to identify the viability of cells, stages of apoptosis, mrna and signaling proteins expression, respectively. |
2012-08-23 |
2023-08-12 |
Not clear |
| Yingmei Wang, Yuanxi Zhu, Lizhi Zhang, Wenyan Tian, Shaofang Hua, Jing Zhao, Huiying Zhang, Fengxia Xu. Insulin promotes proliferation, survival, and invasion in endometrial carcinoma by activating the MEK/ERK pathway. Cancer letters. vol 322. issue 2. 2012-08-20. PMID:22459351. |
the involvement of insulin in endometrial carcinoma (ec) was investigated using radioimmunoassay, western blot, immunoprecipitation, mtt, and annexin v-fitc/pi assays in tissue samples and cultured cells. |
2012-08-20 |
2023-08-12 |
Not clear |
| Mei Liu, Xiao Yang, Jinjin Fan, Rui Zhang, Jun Wu, Youjia Zeng, Jing Nie, Xueqing Y. Altered tight junctions and fence function in NRK-52E cells induced by aristolochic acid. Human & experimental toxicology. vol 31. issue 1. 2012-05-08. PMID:21558304. |
cell viability was detected by mtt, cell apoptosis by flow cytometric analysis, the expression of zonula occludens-1 (zo-1), e-cadherin and polarity scaffold (par3) by western blot and immunofluorescence, cell membrane permeability by transepithelial electrical resistance (teer). |
2012-05-08 |
2023-08-12 |
Not clear |
| Bin Li, Dong-Sung Lee, Gil-Saeng Jeong, Youn-Chul Ki. Involvement of heme oxygenase-1 induction in the cytoprotective and immunomodulatory activities of 6,4'-dihydroxy-7-methoxyflavanone in murine hippocampal and microglia cells. European journal of pharmacology. vol 674. issue 2-3. 2012-05-03. PMID:22119385. |
the effect of dmf on cell viability was determined by mtt assay and the effects of dmf on pro-inflammatory enzymes and cytokines were analyzed by western blot and elisa. |
2012-05-03 |
2023-08-12 |
mouse |
| Silvia Caggia, Massimo Libra, Grazia Malaponte, Venera Cardil. Modulation of YY1 and p53 expression by transforming growth factor-β3 in prostate cell lines. Cytokine. vol 56. issue 2. 2012-01-30. PMID:21807531. |
the expression of yy1, p53, pi3k, akt, pakt, pten, bcl-2, bax, and inos was evaluated through western blot analysis on bph-1, lncap, and du-145 cultures treated with 10 and 50 ng/ml of tgf-β3 for 24 h. the production of nitric oxide (no) was determined by griess reagent and cell viability through mtt assay. |
2012-01-30 |
2023-08-12 |
human |
| Xiao-Feng Li, Jing-He Li, Chun-Hong Wang, Xiu-Li Wang, Qiu-Ju Liu, Wen Xu, Bo Jia, Lin Qiu, Jun M. [Apoptosis of KBM5R cell line with T315I point mutation induced by arsenic trioxide]. Zhongguo shi yan xue ye xue za zhi. vol 19. issue 3. 2012-01-19. PMID:21729541. |
resistance of kbm5r cells to im and proliferation of kbm5 and kbm5r cells treated with ato were detected by mtt; apoptosis of kbm5 and kbm5r cells were quantified by flow cytometry; the expression of apoptosis-related protein caspase-3, -8, -9 was determined by western blot. |
2012-01-19 |
2023-08-12 |
Not clear |