| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Yue Xing, Di Yang, Jing Lu, De-Li Don. Insulin prevents bone morphogenetic protein-4 induced cardiomyocyte apoptosis through activating Akt. Biochemical and biophysical research communications. vol 456. issue 2. 2015-03-13. PMID:25490386. |
cell viability and apoptosis were measured by using mtt, live and dead staining, caspase-3 activity assays, and the protein expressions were measured by using western blot technique. |
2015-03-13 |
2023-08-13 |
Not clear |
| Jiying Jiang, Shuna Yu, Zhengchen Jiang, Cuihong Liang, Wenbo Yu, Jin Li, Xiaodong Du, Hailiang Wang, Xianghong Gao, Xin Wan. N-acetyl-serotonin protects HepG2 cells from oxidative stress injury induced by hydrogen peroxide. Oxidative medicine and cellular longevity. vol 2014. 2015-02-04. PMID:25013541. |
to determine whether pretreatment with nas could reduce hydrogen peroxide- (h2o2-) induced oxidative stress in hepg2 cells by inhibiting the mitochondrial apoptosis pathway, we investigated the h2o2-induced oxidative damage to hepg2 cells with or without nas using mtt, hoechst 33342, rhodamine 123, terminal dutp nick end labeling assay (tunel), dihydrodichlorofluorescein (h2dcf), annexin v and propidium iodide (pi) double staining, immunocytochemistry, and western blot. |
2015-02-04 |
2023-08-13 |
Not clear |
| Wu Hui, Chen Kuisheng, Zhang Hongxin, Ding Yanzhi, Zhao Zhihua, Zhang H. Influence of smoothened siRNA on human esophageal cancer cell line EC9706 proliferation and apoptosis. Pakistan journal of pharmaceutical sciences. vol 27. issue 5 Suppl. 2015-01-22. PMID:25262515. |
utilizing smosirna transfected esophageal squamous carcinoma ec9706 cells, adopting rt-pcr and western blot technology to detect smo, gli1 mrna and protein expression in each group of cells, employing mtt and fcm to detect the influence of smosirna on cell proliferation and apoptosis. |
2015-01-22 |
2023-08-13 |
human |
| Raniere Fagundes Melo-Silveira, Gabriel Pereira Fidelis, Rony Lucas Silva Viana, Vinícius Campelo Soeiro, Rodrigo Augusto da Silva, Daisy Machado, Leandro Silva Costa, Carmen Veríssima Ferreira, Hugo Alexandre Oliveira Roch. Antioxidant and antiproliferative activities of methanolic extract from a neglected agricultural product: corn cobs. Molecules (Basel, Switzerland). vol 19. issue 4. 2015-01-16. PMID:24879583. |
furthermore, its antiproliferative property against tumor cells was assessed through mtt assays and proteins related to apoptosis in tumor cells were examined by western blot. |
2015-01-16 |
2023-08-13 |
cat |
| Koichi Shoji, Jun Teishima, Tetsutaro Hayashi, Shinya Ohara, Wallace L Mckeehan, Akio Matsubar. Restoration of fibroblast growth factor receptor 2IIIb enhances the chemosensitivity of human prostate cancer cells. Oncology reports. vol 32. issue 1. 2015-01-14. PMID:24839986. |
the effects of chemotherapeutic agents (docetaxel, cisplatin, 5-fluorouracil and zoledronic acid) on cell viability and apoptosis were examined by mtt assay and western blot analysis, respectively. |
2015-01-14 |
2023-08-13 |
human |
| Liu Ying, Zhu Zu-An, Liu Qing-Hua, Kong Qing-Yan, Liu Lei, Cui Tao, Wu Yong-Pin. RAD001 can reverse drug resistance of SGC7901/DDP cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. vol 35. issue 9. 2014-12-29. PMID:24920069. |
to investigate the role of rad001 in the reversing of drug resistance of sgc7901/ddp, we cultured sgc7901/ddp cells with different groups of drugs (rad001, cisplatin (ddp) alone, or the combination of rad001 and ddp); after that, we detected the drug sensitivity, cell apoptosis, and levels of p-gp, mrp1, and survivin in the cells of sgc7901/ddp by mtt (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphe-nyltetrazolium bromide) assay, flow cytometry, immunohistochemical analysis, and western blot analysis. |
2014-12-29 |
2023-08-13 |
Not clear |
| Min Chul Kim, Hee Jung Lee, Bora Lim, Ki-Tae Ha, Sung Young Kim, Insuk So, Byung Joo Ki. Quercetin induces apoptosis by inhibiting MAPKs and TRPM7 channels in AGS cells. International journal of molecular medicine. vol 33. issue 6. 2014-12-18. PMID:24647664. |
to investigate the signaling pathway of quercetin-induced apoptosis in the ags cells, western blot analysis and mtt assay were performed. |
2014-12-18 |
2023-08-12 |
human |
| Huanlong Liu, Xueyan Chen, Yu Han, Chaoyi Li, Pingping Chen, Suwen Su, Yongjian Zhang, Zhenhua Pa. Rho kinase inhibition by fasudil suppresses lipopolysaccharide-induced apoptosis of rat pulmonary microvascular endothelial cells via JNK and p38 MAPK pathway. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 68. issue 3. 2014-12-12. PMID:24406296. |
the results suggested that fasudil effectively prevented lps-induced injury of rat pmvecs, as determined by mtt assay, ldh activity assay, apoptosis and western blot analysis of apoptosis-related proteins bcl-2 and bax. |
2014-12-12 |
2023-08-12 |
rat |
| Zhenhua Pei, Fengqiu Zhang, Zhongying Niu, Shenggen Sh. Effect of icariin on cell proliferation and the expression of bone resorption/formation-related markers in human periodontal ligament cells. Molecular medicine reports. vol 8. issue 5. 2014-11-13. PMID:24065204. |
thus, in the present study, the effect of icariin on cell proliferation and the expression of osteoprotegerin (opg), receptor activator of nuclear factor-κb ligand (rankl), core binding factor α1 (cbfa1) and osteocalcin (oc) was investigated in human periodontal ligament cells, by an mtt assay, qpcr and western blot analysis. |
2014-11-13 |
2023-08-12 |
human |
| S U Kang, J-H Cho, J W Chang, Y S Shin, K I Kim, J K Park, S S Yang, J-S Lee, E Moon, K Lee, C-H Ki. Nonthermal plasma induces head and neck cancer cell death: the potential involvement of mitogen-activated protein kinase-dependent mitochondrial reactive oxygen species. Cell death & disease. vol 5. 2014-10-21. PMID:24525732. |
the effects of ntp on apoptosis were investigated using mtt, terminal deoxynucleotidyl transferase-mediated dutp-biotin nick-end labeling, annexin v assays, and western blot analysis. |
2014-10-21 |
2023-08-12 |
mouse |
| Gongbo Li, Sakina M Petiwala, Dana R Pierce, Larisa Nonn, Jeremy J Johnso. Selective modulation of endoplasmic reticulum stress markers in prostate cancer cells by a standardized mangosteen fruit extract. PloS one. vol 8. issue 12. 2014-10-08. PMID:24367485. |
flow cytometry, mtt, brdu and western blot were used to evaluate cell apoptosis, viability, proliferation and er stress. |
2014-10-08 |
2023-08-12 |
mouse |
| Ting Zou, Xiangqi Tang, Zhiling Huang, Niangui Xu, Zhiping H. The Pael-R gene does not mediate the changes in rotenone-induced Parkinson's disease model cells. Neural regeneration research. vol 9. issue 4. 2014-09-10. PMID:25206827. |
the results of reverse-transcription polymerase chain reaction and western blot analysis showed that pael-r expression was decreased after rna interference compared with the control group (no treatment) and the model group (rotenone treatment), while the rate of apoptosis and survival of dopaminergic cells did not differ significantly between groups, as detected by flow cytometry and an mtt assay. |
2014-09-10 |
2023-08-13 |
Not clear |
| Ravikanth Danda, Gopinath Krishnan, Kalaivani Ganapathy, Uma Maheswari Krishnan, Khetan Vikas, Sailaja Elchuri, Nivedita Chatterjee, Subramanian Krishnakuma. Targeted expression of suicide gene by tissue-specific promoter and microRNA regulation for cancer gene therapy. PloS one. vol 8. issue 12. 2014-08-25. PMID:24391761. |
the cell proliferation was measured by mtt assay, apoptosis was measured by probing cleaved caspase3, epcam and tk expression were quantified by western blot. |
2014-08-25 |
2023-08-12 |
Not clear |
| Abida Arshad, Xuechai Chen, Zhenzhen Cong, Hong Qing, Yulin Den. TRPC1 protects dopaminergic SH-SY5Y cells from MPP+, salsolinol, and N-methyl-(R)-salsolinol-induced cytotoxicity. Acta biochimica et biophysica Sinica. vol 46. issue 1. 2014-08-04. PMID:24252728. |
mtt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays, dapi (4',6-diamidino-2-phenylindole), fluorescein isothiocyanate-annexin-v/propidium iodide, western blot analysis, and jc-1 labeling revealed that the three indicated drugs could induce caspase-dependent, mitochondrial-mediated apoptosis. |
2014-08-04 |
2023-08-12 |
Not clear |
| Rui Zeng, Yan Xiong, Fengming Zhu, Zufu Ma, Wenhui Liao, Yong He, JinSeng He, Wei Li, Juan Yang, Qian Lu, Gang Xu, Ying Ya. Fenofibrate attenuated glucose-induced mesangial cells proliferation and extracellular matrix synthesis via PI3K/AKT and ERK1/2. PloS one. vol 8. issue 10. 2014-06-24. PMID:24130796. |
in this study, the effect of fenofibrate on high-glucose induced cell proliferation and extracellular matrix exertion and its mechanisms were investigated in cultured rat mesangial cells by the methylthiazoletetrazolium (mtt) assay, flow cytometry and western blot. |
2014-06-24 |
2023-08-12 |
rat |
| Chuanyu Sun, Shuai Xu, Jia Guo, Ke Xu, Changchun Wan. The inhibitory and apoptotic effects of docetaxel-loaded mesoporous magnetic colloidal nanocrystal clusters on bladder cancer T24 cells in vitro. Journal of biomedical nanotechnology. vol 10. issue 3. 2014-06-12. PMID:24730241. |
a series of analyses for cell uptake, cell viability, cell cycle, cell apoptosis and some cell proteins were performed by transmission electron microscopy, mtt assay, flow cytometry, cell nuclei staining, annexin v staining assay, western blot assay and caspase-3 activity assay, respectively. |
2014-06-12 |
2023-08-13 |
Not clear |
| Jingjing Kong, Guiru Ren, Ning Jia, Yanfu Wang, Hua Zhang, Wei Zhang, Bingkun Chen, Yunpeng Ca. Effects of nicorandil in neuroprotective activation of PI3K/AKT pathways in a cellular model of Alzheimer's disease. European neurology. vol 70. issue 3-4. 2014-06-11. PMID:24008440. |
the cells were then collected for mtt, apoptosis assay, and western blot. |
2014-06-11 |
2023-08-12 |
Not clear |
| Hao Li, Xin Xiang Li, Qing Ma, Jia Cu. The variability of oxLDL-induced cytotoxicity on different types of cell lines. Cell biochemistry and biophysics. vol 67. issue 2. 2014-06-07. PMID:23479334. |
huvec, k562/ao2 and ec-9706 cell lines were cultured in the presence of oxldl, and cell proliferation was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) assay, apoptosis and cell cycle by flow cytometer, mrna expression by rt-pcr and protein expression by western blot. |
2014-06-07 |
2023-08-12 |
human |
| Yahong Zhao, Weijia Zhao, Shu Yu, Yibing Guo, Xiaosong Gu, Yumin Yan. Biocompatibility evaluation of electrospun silk fibroin nanofibrous mats with primarily cultured rat hippocampal neurons. Bio-medical materials and engineering. vol 23. issue 6. 2014-05-30. PMID:24165556. |
mtt assay and immunocytochemistry in couple with western blot analysis respectively indicated there was no significant difference in both the cell viability and expression levels of some proteins, including gap-43, map-2, nf, and β-tubulin, between hippocampal neurons cultured in the electrospun sf nanofibrous mat extract and in plain neuronal medium. |
2014-05-30 |
2023-08-12 |
rat |
| Hui-Qing Zhang, Bo He, Nian Fang, Shan Lu, Yu-Qian Liao, Yi-Ye Wa. Autophagy inhibition sensitizes cisplatin cytotoxicity in human gastric cancer cell line SGC7901. Asian Pacific journal of cancer prevention : APJCP. vol 14. issue 8. 2014-05-15. PMID:24083726. |
after sgc7901 cells were treated with ddp and/or chloroquine, cell proliferation was measured using mtt assay; cell apoptosis was determined by flow cytometry; autophagy and apotosis-related proteins expression were detected by western blot; and quantitative analysis of autophagy after monodansylcadaverine (mdc) staining was performed using fluorescence microscopy. |
2014-05-15 |
2023-08-12 |
human |