| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Che-Yi Chao, Chong-Kuei Lii, Siou-Yu Ye, Chien-Chun Li, Chia-Yang Lu, Ai-Hsuan Lin, Kai-Li Liu, Haw-Wen Che. Docosahexaenoic acid inhibits vascular endothelial growth factor (VEGF)-induced cell migration via the GPR120/PP2A/ERK1/2/eNOS signaling pathway in human umbilical vein endothelial cells. Journal of agricultural and food chemistry. vol 62. issue 18. 2015-11-10. PMID:24734983. |
huvecs were used as the study model, and the mtt assay, western blot, wound-healing assay, and phosphatase activity assay were used to explore the effects of dha on cell migration. |
2015-11-10 |
2023-08-13 |
human |
| Jong Seok Lee, Young Rae Kim, Jun Myoung Park, Young Eon Kim, Nam In Baek, Eock Kee Hon. Cyanidin-3-glucoside isolated from mulberry fruits protects pancreatic β-cells against glucotoxicity-induced apoptosis. Molecular medicine reports. vol 11. issue 4. 2015-09-16. PMID:25501967. |
in addition, the effects of c3g on the glucotoxicity‑induced apoptosis of pancreatic β‑cells was evaluated using mtt assay, immunofluorescent staining, flow cytometric and western blot analyses. |
2015-09-16 |
2023-08-13 |
Not clear |
| Dong Hoon Kwak, Ji-Hye Park, Hak-Seung Lee, Ji-Sook Moon, Seoul Le. Aristolochic Acid I induces ovarian toxicity by inhibition of akt phosphorylation. Chemical research in toxicology. vol 27. issue 12. 2015-08-21. PMID:25406029. |
we used chinese hamster ovary-k1 (cho-k1) cells and an aai injection mouse model: mtt assay was used to assess aa toxicity to cells; ovary size and weight were measured to determine the toxicity of aa to mouse ovary; western blot was used to assess apoptosis; tunel assay was used to evaluate apoptotic cell death; and immunohistochemistry was used to examine the local expression of apoptotic proteins in ovary tissue. |
2015-08-21 |
2023-08-13 |
mouse |
| Sze-Kwan Lam, Yuan-Yuan Li, Chun-Yan Zheng, James Chung-Man H. Downregulation of thymidylate synthase and E2F1 by arsenic trioxide in mesothelioma. International journal of oncology. vol 46. issue 1. 2015-08-11. PMID:25335113. |
a panel of 5 mesothelioma cell lines was used to study the effect of ato on cell viability, protein expression, mrna expression and tyms activity by mtt assay, western blot, qpcr and tritium-release assay, respectively. |
2015-08-11 |
2023-08-13 |
mouse |
| Cunjin Su, Aiming Shi, Guowen Cao, Tao Tao, Ruidong Chen, Zhanhong Hu, Zhu Shen, Hong Tao, Bin Cao, Duanmin Hu, Junjie Ba. Fenofibrate suppressed proliferation and migration of human neuroblastoma cells via oxidative stress dependent of TXNIP upregulation. Biochemical and biophysical research communications. vol 460. issue 4. 2015-07-27. PMID:25839662. |
we used mtt assay to detect cells proliferation, starch wound test to investigate cells migration, h2dcf-da to detect intracellular ros, sirna to interfere txnip and peroxisome proliferator-androgen receptor-alpha (ppar-α) expression, western blot to determine protein levels, flow cytometry to analyze apoptosis. |
2015-07-27 |
2023-08-13 |
human |
| Kanjoormana A Manu, Muthu K Shanmugam, Lalitha Ramachandran, Feng Li, Kodappully Sivaraman Siveen, Arunachalam Chinnathambi, M E Zayed, Sulaiman Ali Alharbi, Frank Arfuso, Alan Prem Kumar, Kwang Seok Ahn, Gautam Seth. Isorhamnetin augments the anti-tumor effect of capecitabine through the negative regulation of NF-κB signaling cascade in gastric cancer. Cancer letters. vol 363. issue 1. 2015-07-20. PMID:25827070. |
the potential effect of ih on viability was analyzed by mtt assay, apoptosis by flow cytometric analysis, and nf-κb activation by dna binding as well as western blot assays. |
2015-07-20 |
2023-08-13 |
mouse |
| Jing Wang, Hong Wei, Baoxia Zhao, Mei Li, Weipeng Lv, Ling Lv, Bo Song, Shen L. The reverse effect of X-ray irradiation on acquired gefitinib resistance in non-small cell lung cancer cell line NCI-H1975 in vitro. Journal of molecular histology. vol 45. issue 6. 2015-07-15. PMID:25008024. |
in the three cell lines, subsequently cell growth curves and cell population doubling time were calculated by cell proliferation assay, the changes of cell viability were evaluated by trypan blue dye exclusion method and mtt assay, the cell cycle distribution and apoptosis were investigated by flow cytometry, the expressions of e-cadherin and vimentin used to indicate epithelial-mesenchymal transition (emt) were determined by western blot analysis, the protein expressions in egfr/kras/braf transduction pathway were detected by immunocytochemistry, and the mutations of egfr, kras and braf were detected by high resolution melting analysis and direct sequencing. |
2015-07-15 |
2023-08-13 |
human |
| Wen-Xiao Xu, Yang Liu, Sheng-Zhi Liu, Yu Zhang, Guo-Fen Qiao, Jinglong Ya. Arsenic trioxide exerts a double effect on osteoblast growth in vitro. Environmental toxicology and pharmacology. vol 38. issue 2. 2015-06-23. PMID:25128771. |
cell survives, apoptosis, collagen, and molecular targets were identified by multiple detecting techniques, including mtt assay, electron microscopy, collagen detecting kit, tunel kit, and western blot in hfob1.19 human osteoblasts cell line. |
2015-06-23 |
2023-08-13 |
human |
| Wei Tan, Hong-Gang Yu, He-Sheng Lu. Inhibition of the p38 MAPK pathway sensitizes human gastric cells to doxorubicin treatment in vitro and in vivo. Molecular medicine reports. vol 10. issue 6. 2015-06-19. PMID:25270341. |
the effect of doxorubicin on the growth and apoptosis of gastric cancer cells in the presence or absence of sb203580 was investigated by western blot analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, hoechst staining, annexin v-fitc/propidium iodide staining followed by flow cytometry analysis, and the terminal deoxynucleotidyl transferase-mediated dutp nick end labeling (tunel) assay. |
2015-06-19 |
2023-08-13 |
human |
| Yan Jing Li, Peng Huang, Cai Ling Jiang, De Xin Jia, Xiao Xue Du, Jian Hua Zhou, Yu Han, Hong Sui, Xiao Li Wei, Lei Liu, Heng Heng Yuan, Ting Ting Zhang, Wen Jie Zhang, Rui Xie, Xiao Hui Lang, Li Ying Wang, Tao Liu, Yu Xian Bai, Ye Tia. Sonodynamically induced anti-tumor effect of 5-aminolevulinic acid on pancreatic cancer cells. Ultrasound in medicine & biology. vol 40. issue 11. 2015-06-18. PMID:25220273. |
cell viability was determined by mtt (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) analysis; mitochondrial membrane potential was assessed using the fluorescent probe jc-1; apoptosis was evaluated by flow cytometry; cell morphology was investigated by scanning electron microscopy; apoptosis-related protein expression was analyzed by western blot assay. |
2015-06-18 |
2023-08-13 |
human |
| Taofeng Zhu, Ruhua Chen, Hao Yu, Yan Feng, Jianqiang Chen, Qin Lu, Jing Xie, Weiliang Ding, Tieliang M. Antitumor effect of a copper (II) complex of a coumarin derivative and phenanthroline on lung adenocarcinoma cells and the mechanism of action. Molecular medicine reports. vol 10. issue 5. 2015-06-09. PMID:25176185. |
an mtt assay was performed to determine the cell proliferation ratio, cell apoptosis was detected by annexin v/propidium iodide staining with flow cytometric analysis and western blot analysis was employed to evaluate the expression levels of apoptosis-associated proteins. |
2015-06-09 |
2023-08-13 |
mouse |
| Xu-Hui Zhou, Chao-Qun Yang, Cheng-Lin Zhang, Yang Gao, Hong-Bin Yuan, Ce Wan. RASSF5 inhibits growth and invasion and induces apoptosis in osteosarcoma cells through activation of MST1/LATS1 signaling. Oncology reports. vol 32. issue 4. 2015-06-01. PMID:25109282. |
a gain-of-function approach was used to observe the effects of lentiviral vector-mediated overexpression of rassf5 (lv-rassf5) on cell growth, invasion and apoptosis, respectively, as indicated by mtt, transwell and flow cytometry assays, and the expression levels of mammalian sterile 20-like (mst1) kinase, large tumor suppressor 1 (lats1), proliferating cell nuclear antigen (pcna), matrix metallopeptidase-9 (mmp-9) and p53 were detected by real-time pcr and western blot assays in os cells (mg-63 and u-2 os). |
2015-06-01 |
2023-08-13 |
human |
| Xing Ai, Zhuomin Jia, Shuanglin Liu, Jiajun Wang, Xu Zhan. Notch-1 regulates proliferation and differentiation of human bladder cancer cell lines by inhibiting expression of Krüppel-like factor 4. Oncology reports. vol 32. issue 4. 2015-06-01. PMID:25109409. |
cell proliferation, apoptosis and expression of notch-1 and klf4 were assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, flow cytometry assay with annexin v-fitc/pi staining, and reverse transcriptase polymerase chain reaction (rt-pcr) and western blot analysis, respectively. |
2015-06-01 |
2023-08-13 |
human |
| Shingo Takano, Eiichi Ishikawa, Masahide Matsuda, Tetsuya Yamamoto, Akira Matsumur. Interferon-β inhibits glioma angiogenesis through downregulation of vascular endothelial growth factor and upregulation of interferon inducible protein 10. International journal of oncology. vol 45. issue 5. 2015-05-25. PMID:25175315. |
the effect of ifn-β for these cell lines were evaluated by means of proliferation (mtt assay), conditioned medium induced huvec migration, vegf and interferon inducible protein 10 (ip10, angiogenesis inhibitor) expression by rt-pcr and western blot analysis. |
2015-05-25 |
2023-08-13 |
mouse |
| Bo Huang, Hongli Zhou, Xianping Lang, Zhiliang Li. siRNA‑induced ABCE1 silencing inhibits proliferation and invasion of breast cancer cells. Molecular medicine reports. vol 10. issue 4. 2015-05-22. PMID:25070080. |
the effect of abce1 knockdown on cell proliferation, invasion, apoptosis and gene expression was then assessed using mtt assay, transwell migration assay, flow cytometry and western blot analysis, respectively. |
2015-05-22 |
2023-08-13 |
human |
| Hong-Nan Huo, Kun-Peng Xie, Li-Meng Wang, Ying Jiang, Wei Zou, Ming-Jie Xi. [Relationship between the inhibitory effect of fraxetin on breast cancer and estrogen signaling pathway]. Sheng li xue bao : [Acta physiologica Sinica]. vol 65. issue 3. 2015-05-16. PMID:23788190. |
the article used mtt assay, flow cytometer analysis and western blot to detect the inhibition of fraxetin on mcf-7 cell cycle distribution and apoptosis, erα, cyclin d1 and bcl-2 expression levels, mapk and pi3k signaling pathway to investigate the mechanism of anti-breast cancer of fraxetin. |
2015-05-16 |
2023-08-12 |
Not clear |
| Ping Zhang, He-Sheng Luo, Ming Li, Shi-Yun Ta. Artesunate inhibits the growth and induces apoptosis of human gastric cancer cells by downregulating COX-2. OncoTargets and therapy. vol 8. 2015-05-06. PMID:25945055. |
the effects of artesunate on the growth and apoptosis of gastric cancer cells were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, flow cytometric analysis of annexin v-fluorescein isothiocyanate/propidium iodide staining, rhodamine 123 staining, and western blot analysis. |
2015-05-06 |
2023-08-13 |
human |
| Yong-Zhan Zhen, Ya-Jun Lin, Kai-Ji Li, Xiao-Shan Yang, Yu-Fang Zhao, Jie Wei, Jing-Bo Wei, Gang H. Gambogic Acid lysinate induces apoptosis in breast cancer mcf-7 cells by increasing reactive oxygen species. Evidence-based complementary and alternative medicine : eCAM. vol 2015. 2015-04-13. PMID:25866542. |
inhibition of cell proliferation was determined by mtt assay; intracellular ros level was detected by staining cells with dcfh-da; cell apoptosis was determined by flow cytometer and the mechanism of gal was investigated by western blot. |
2015-04-13 |
2023-08-13 |
human |
| Jia-Xin Fan, Ying-Jian Zeng, Jian-Wei Wu, Zhang-Qiu Li, Yuan-Ming Li, Rong Zheng, Guang-Yang Weng, Kun-Yuan Gu. [Synergistic killing effect of arsenic trioxide combined with curcumin on KG1a cells]. Zhongguo shi yan xue ye xue za zhi. vol 22. issue 5. 2015-03-30. PMID:25338570. |
the cell survival rate was mesured by mtt; colony formation capacity was examined by methylcellulose colony formation test; flow cytometry was used to analyse the cell surface molecules, cell apoptosis rate and cell cycle; the cell morphology was observed with wright-giemsa staining and the protein expression of bcl-2, bax, parp was detected by western blot. |
2015-03-30 |
2023-08-13 |
Not clear |
| Ying Wang, Yu-Xiang Han, Zhi-Yun Niu, Xing-Zhe Wang, Huan Hua, Yin-Tao Shang, Fu-Xu Wang, Xue-Jun Zhang, Jian-Min Lu. [BMMSC from blastic phase CML down-regulate leukemia cell apoptosis]. Zhongguo shi yan xue ye xue za zhi. vol 22. issue 5. 2015-03-30. PMID:25338597. |
k562 cells and primary cml-bp cells were co-cultured with bmmsc of different groups; the cell proliferation was detected by mtt method, the cell apoptosis rate and mitochondrial membrane potential were measured by flow cytometry, the expression levels of caspase-8, caspase-9, and activated caspase-3 in cells were measured by western blot. |
2015-03-30 |
2023-08-13 |
Not clear |