| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Sujun Huang, Binwen Wu, Dongfeng Li, Weihong Zhou, Gang Deng, Kaijun Zhang, Youjia L. Knockdown of astrocyte elevated gene-1 inhibits tumor growth and modifies microRNAs expression profiles in human colorectal cancer cells. Biochemical and biophysical research communications. vol 444. issue 3. 2014-05-06. PMID:24462870. |
their phenotypic changes were analyzed by western blot, mtt and transwell matrix penetration assays. |
2014-05-06 |
2023-08-12 |
human |
| Si-Qun Wen, Liang-Ming Ma, Yu-Jin Lu, Bo Ba. [Triptolide inhibits proliferation and induces apoptosis of imatinib resistant K562/G01 cells]. Zhongguo shi yan xue ye xue za zhi. vol 21. issue 5. 2014-05-05. PMID:24156423. |
mtt assay was used to detect the effect of imatinib or triptolide alone and their combination on k562/g01 proliferation; the cell cycle, apoptosis rate, p-gp protein expression were detected by flow cytometry (fcm); the expression of p-gp was assessed by western blot; the bcr/abl gene expression was assayed by real time quantitative pcr. |
2014-05-05 |
2023-08-12 |
Not clear |
| Hong-Pu Chen, Yi-Qun Huang, Xu-Dong M. [Antiproliferative effects of LY294002 on MCL Jeko-1 cell line and its mechanism]. Zhongguo shi yan xue ye xue za zhi. vol 21. issue 5. 2014-05-05. PMID:24156430. |
the proliferation inhibitory rate of jeko-1 cells treated by different doses of ly294002 was assayed by mtt method; the level of apoptosis of jeko-1 cells was detected by flow cytometry; the expression level of apoptosis-related protein cyclin d1, bcl-2, procaspase-3 and pi3k/akt signaling pathway protein phosphorylated-akt (p-akt), phosphorylated-tor (p-mtor), phosphorylated-p70s6k (p-p70s6k) phosphorylated-akt (p-akt) in jeko-1 cells were determined by western blot. |
2014-05-05 |
2023-08-12 |
Not clear |
| Ya-Juan Chen, Hui-Min Li, Wei Lu, Chen Qin. [Blocking effect of arsenic trioxide on the proliferation and cell cycle of human Burkitt lymphoma cells and its related mechanism]. Zhongguo shi yan xue ye xue za zhi. vol 21. issue 6. 2014-05-01. PMID:24370028. |
human burkitt lymphoma cell line namalwa was used as the model, the effect of as2o3 on cell proliferation, cell cycle and apoptosis, as well as the expression of cell cycle modulation related genes, including mrna and protein level, were detected by mtt method, flow cytometry, real-time quantitative pcr (rq-pcr) and western blot, respectively. |
2014-05-01 |
2023-08-12 |
human |
| Rong Sun, Ke Wang, Dongdong Wu, Xiaoyu Li, Yangbing O. Protective effect of paeoniflorin against glutamate-induced neurotoxicity in PC12 cells via Bcl-2/Bax signal pathway. Folia neuropathologica. vol 50. issue 3. 2014-04-14. PMID:23023341. |
pc12 cells were cultured in vitro, cell viability was assessed by mtt assay, cell apoptosis as well as mitochondrial membrane potential (mmp) were detected by flow cytometric analysis and the expression profiles of apoptosis-related proteins including bcl-2 and bax were investigated by western blot. |
2014-04-14 |
2023-08-12 |
rat |
| Jin Rhyu, Min Sook Kim, Mi-Kyoung You, Mi-Ae Bang, Hyeon-A Ki. Pear pomace water extract inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes. Nutrition research and practice. vol 8. issue 1. 2014-03-10. PMID:24611103. |
the effects of pear pomace extract on cell viability and the anti-adipogenic and proapoptotic effects were investigated via mtt assay, oil red o staining, western blot analysis and apoptosis assay. |
2014-03-10 |
2023-08-12 |
Not clear |
| S Zhang, Y Qi, H Zhang, W He, Q Zhou, S Gui, Y Wan. Melatonin inhibits cell growth and migration, but promotes apoptosis in gastric cancer cell line, SGC7901. Biotechnic & histochemistry : official publication of the Biological Stain Commission. vol 88. issue 6. 2014-02-10. PMID:23477595. |
we investigated the effects of melatonin on cell proliferation, apoptosis, colony formation and cell migration in the gastric adenocarcinoma cell line, sgc7901, using mtt assay, hoechst 33258 staining, flow cytometry, western blot, caspase-3 activity assay, soft agar colony formation assay, and scratch-wound assay. |
2014-02-10 |
2023-08-12 |
Not clear |
| Li-Lin Ye, Wei-Ke Cao, Yu-Ye Shi, Zhi-Kui Deng, Shan-Dong Tao, Ping Ji, Xian-Hua Fu, Ming-Zhu Zong, Yu-Feng L. [Effect of homoharringtonine on expression of NF-κB and BCL-2 proteins in K562 cells]. Zhongguo shi yan xue ye xue za zhi. vol 21. issue 1. 2013-12-16. PMID:23484696. |
the cells proliferation was assayed with mtt method, the cell apoptosis, cell cycle and bcl-2 expression were analyzed with flow cytometry, nf-κb protein expression was detected with western blot. |
2013-12-16 |
2023-08-12 |
Not clear |
| Bao-Lan Liu, Xin Liu, Nai-Cen Zhou, Mei-Ying Qi, Bo X. [Mechanisms of oxaliplatin-induced apoptosis of human multiple myeloma cell RPMI8226]. Zhongguo shi yan xue ye xue za zhi. vol 21. issue 1. 2013-12-16. PMID:23484700. |
the proliferation inhibitory rate of rpmi8226 cells was assayed by mtt, the morphological changes of rpmi-8226 cells were observed by inverted fluorescent microscopy and transmission electron microscopy, the apoptosis rate and the cell cycle distribution of rpmi-8226 cells were detected by flow cytometry, the effects of oxaliplatin on the expression of bcl-2, caspase-8, caspase-3 mrna were tested by rt-pcr, bcl-2 protein expression of rpmi-8226 cells was analyzed by western blot. |
2013-12-16 |
2023-08-12 |
human |
| Yi Huang, Jian-Da Hu, Yan-An Wu, Jing Zheng, Yuan-Lin Qi, Ying-Yu Chen, Xiao-Li Huan. [Effects of eEF1A1 re-expression on proliferation and apoptosis of Jurkat cells with knocked down eEF1A1 gene and its mechanisms]. Zhongguo shi yan xue ye xue za zhi. vol 21. issue 2. 2013-12-16. PMID:23628016. |
then, the expressions of eef1a1 mrna and protein were detected by real time pcr(rt-pcr) and western blot respectively.cell proliferation, apoptosis and cycle were detected by mtt method, annexin v-apc labeling and dna ploidy analysis respectively. |
2013-12-16 |
2023-08-12 |
human |
| Ling Zhang, Yan-Ping Ma, Gu Jia, Yu-Jin Lu, Li Zhang, Hong Lu, Ming-Xing Chan. [Inhibitory effect of histone deacetylase inhibitor LBH589 on multiple myeloma MM1R cells in vitro]. Zhongguo shi yan xue ye xue za zhi. vol 20. issue 5. 2013-12-05. PMID:23114131. |
the effect of lbh589 (10, 20, 50 nmol/l) alone or combined with bor (10, 20 nmol/l) on mm1r proliferation was detected by mtt method; the effect of lbh589 on cell cycle and apoptosis of mm1r cells were determined by flow cytometry; the histone h4 acetylation level of mm1r cells treated with lbh589 (10, 20, 50 nmol/l) for 24 h was analyzed by western blot. |
2013-12-05 |
2023-08-12 |
Not clear |
| Lei Xia, Wei Guan, Dong Wang, Yun-Song Zhang, Lin-Li Zeng, Zeng-Peng Li, Ge Wang, Zhen-Zhou Yan. Killing effect of Ad5/F35-APE1 siRNA recombinant adenovirus in combination with hematoporphrphyrin derivative-mediated photodynamic therapy on human nonsmall cell lung cancer. BioMed research international. vol 2013. 2013-11-12. PMID:23509821. |
by using the technologies of mtt, flow cytometry, elisa, and western blot, we observed that the proliferation inhibition and apoptosis of the a549 cells were significantly higher than the control group (p < 0.05) after hpd-pdt was performed. |
2013-11-12 |
2023-08-12 |
human |
| Yun-Hee Sung, Mal-Soon Shin, Il-Gyu Ko, Sung-Eun Kim, Chang-Ju Kim, Hyun-Jong Ahn, Hye-Sun Yoon, Bong-Jae Le. Ulinastatin suppresses lipopolysaccharide-induced prostaglandin E2 synthesis and nitric oxide production through the downregulation of nuclear factor‑κB in BV2 mouse microglial cells. International journal of molecular medicine. vol 31. issue 5. 2013-10-29. PMID:23546639. |
to accomplish this, we performed a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, reverse transcription-polymerase chain reaction (rt-pcr), western blot analysis, electrophoretic mobility gel shift assay (emsa), prostaglandin e(2) (pge(2)) immunoassay and nitric oxide (no) detection. |
2013-10-29 |
2023-08-12 |
mouse |
| Xiao-Dong Sun, Xing-E Liu, Dong-Sheng Huan. Curcumin reverses the epithelial-mesenchymal transition of pancreatic cancer cells by inhibiting the Hedgehog signaling pathway. Oncology reports. vol 29. issue 6. 2013-10-29. PMID:23563640. |
the growth inhibition rate of the cells was measured by mtt assay, apoptosis was detected by flow cytometry, the expression levels of shh, gli1, e-cadherin and vimentin were detected by western blot analysis, and cell invasion and migration ability were examined by transwell cell invasion assay and wound healing assay. |
2013-10-29 |
2023-08-12 |
Not clear |
| An-Ming Tang, Wei-Juan Wang, Bin Mei, Wang-Lai Hu, Mian Wu, Gao-Lin Lian. DEVD-based hydrogelator minimizes cellular apoptosis induction. Scientific reports. vol 3. 2013-10-24. PMID:23673405. |
3-(4,5-dimethylthiazol-2-yl) 2,5 diphenyl tetrazolium bromide (mtt) and western blot analyses indicated that dedv-based hydrogelator 2 induces cell death via apoptotic pathway while the devd-based hydrogelator 1 minimizes cellular apoptosis induction. |
2013-10-24 |
2023-08-12 |
Not clear |
| Ying Liu, Zu-an Zhu, Shang-Nuan Zhang, Jie Mou, Lei Liu, Tao Cui, Dong-Sheng Pe. Combinational effect of PPARγ agonist and RXR agonist on the growth of SGC7901 gastric carcinoma cells in vitro. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. vol 34. issue 4. 2013-09-26. PMID:23605322. |
then, the cell growth, apoptosis, morphological changes, and the expression of pparγ, rxrγ, bcl-2, and bax were detected by mtt assay, flow cytometry, he staining, immunocytochemistry staining, and western blot assay, respectively. |
2013-09-26 |
2023-08-12 |
Not clear |
| Yuan Gao, Zan Liu, Xiuwei Zhang, Jiani He, Yongqi Pan, Fengxia Hao, Lingling Xie, Qingchang Li, Xueshan Qiu, Enhua Wan. Inhibition of cytoplasmic GSK-3β increases cisplatin resistance through activation of Wnt/β-catenin signaling in A549/DDP cells. Cancer letters. vol 336. issue 1. 2013-08-15. PMID:23673211. |
the regulation of cisplatin resistance, apoptosis, β-catenin and survivin protein expression by inhibition of cytoplasmic gsk-3β were determined by mtt assay, flow cytometry analysis, immunofluorescence technique and western blot analysis. |
2013-08-15 |
2023-08-12 |
human |
| Dae-Young Lee, Mi-Kyoung Lee, Geum-Soog Kim, Hyung-Jun Noh, Min-Ho Le. Brazilin inhibits growth and induces apoptosis in human glioblastoma cells. Molecules (Basel, Switzerland). vol 18. issue 2. 2013-07-09. PMID:23429418. |
cell proliferation was investigated by mtt assays and growth curve analysis, apoptosis was assessed by facs analysis and western blot studies. |
2013-07-09 |
2023-08-12 |
human |
| Xiaoqin Mu, Kaiwen He, Hui Sun, Xin Zhou, Lingling Chang, Xin Li, Wenfeng Chu, Guofen Qiao, Yanjie L. Hydrogen peroxide induces overexpression of angiotensin-converting enzyme in human umbilical vein endothelial cells. Free radical research. vol 47. issue 2. 2013-06-25. PMID:23153326. |
we used tetrazolium bromide (mtt) assay for cell viability, terminal deoxynucleotidyl transferase-mediated dutp nick end labeling (tunel) assay for cell apoptosis, enzyme-linked immunosorbent assay (elisa) for camp measurement, real-time pcr for mrna detection, and western blot for protein analysis in the study. |
2013-06-25 |
2023-08-12 |
human |
| Hai-Bo Liu, Mei Zhang, Cheng-Liang Li, Peng-Cheng H. [Effect of clofarabine on proliferation and Bcl-2 expression of NB4 cells]. Zhongguo shi yan xue ye xue za zhi. vol 20. issue 3. 2013-05-16. PMID:22739157. |
mtt method was used to detect proliferation of nb4 cells treated with clofarabine 0.01 - 0.1 µmol/l for 48 h. the treated with clofarabine 0.01 - 0.1 µmol/l for 24 h, apoptosis rate and bcl-2 expression of nb4 cells were measured by flow cytometry and western blot respectively. |
2013-05-16 |
2023-08-12 |
Not clear |