| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Ping Yang, Jing Wang, Hong-Mei Jing, Xiao-Yan K. [Effect of magnetic nanoparticle of Fe3O4 combined with daunorubicin on lymphoma cell line Raji]. Zhongguo shi yan xue ye xue za zhi. vol 19. issue 2. 2012-01-12. PMID:21518493. |
the raji cells were cultured with fe3o4-magnetic nanoparticle and daunorubicin polymerized by means of mechanical absorption, the cell viability was detected by mtt and trypan-blue exclusion; the apoptosis was detected by flow cytometry, the expressions of p53 and p65 are measured by western blot. |
2012-01-12 |
2023-08-12 |
Not clear |
| Xiaoqing Guo, Guangzhi Liu, Isaiah G Schauer, Gong Yang, Imelda Mercado-Uribe, Fan Yang, Shiwu Zhang, Yuanli He, Jinsong Li. Overexpression of the β subunit of human chorionic gonadotropin promotes the transformation of human ovarian epithelial cells and ovarian tumorigenesis. The American journal of pathology. vol 179. issue 3. 2011-12-09. PMID:21763678. |
immortalized nontumorigenic ovarian epithelial t29 and t80 cells stably overexpressing β-hcg were examined for alterations in cell cycle and apoptotic status by flow cytometry, expression of proteins regulating cell cycle and apoptosis by western blot, proliferation status by mtt assay, anchorage-independent colony formation, and mouse tumor formation. |
2011-12-09 |
2023-08-12 |
mouse |
| Hui Zhao, Qing-Xian Bai, Gao-Sheng Huang, Li-Jie Yang, Peng Yue, Xiao-Yan Zhang, Lu Wang, Lin-Ni Fan, Jin Zh. [Bortezomib depresses osteoblast apoptosis induced by mouse myeloma cells]. Zhongguo shi yan xue ye xue za zhi. vol 18. issue 5. 2011-10-13. PMID:21129258. |
the inhibitory effect of bzb on proliferation of mc-3t3e1 assayed by modified mtt method, the apoptosis of mc-3t3e1 cells was determined by flow cytometry with annexin v/pi staining, the expressions of osteoblast markers, runx2/cbfa1, osteocalcin (ocn) and osterix (osx) in mc-3t3e1 treated with bzb were detected by rt-pcr and western blot respectively. |
2011-10-13 |
2023-08-12 |
mouse |
| Fu-Xu Wang, Fang Zhao, Ling Pan, Xue-Jun Zhang, Jian-Min Luo, Jing-Ci Yang, Li Yao, Xing-Yan DU, Zuo-Ren Don. [Preliminary study on the effect of TRAIL on adhesion and apoptosis of multiple myeloma cell line RPMI8226 and its mechanism]. Zhongguo shi yan xue ye xue za zhi. vol 18. issue 1. 2011-10-06. PMID:20137126. |
the inhibition effects of trail on cells growth and adhesion were assayed by mtt; cell apoptosis was detected by annexin v and pi; expression of genes bax, bcl-2, mcl-1, carp1, carp2, xiap and cflip were determined by semi-quantitative rt-pcr; apoptosis-related protein expression was detected by western blot. |
2011-10-06 |
2023-08-12 |
Not clear |
| Yong-Lan Ma, Hong Yi, Fei-Lan Yang, Yuan Zhang, Ya Wang, Ying Wan. [Celastrol down-regulates expression of P-Akt and cyclin D1 in HL-60 cells and induces apoptosis]. Zhongguo shi yan xue ye xue za zhi. vol 18. issue 4. 2011-09-30. PMID:20723296. |
the proliferative activity of hl-60 cells treated with 0.25 - 8.0 μmol/l of celastrol for 24 - 72 hours was assayed by mtt method, the effects of celastrol on apoptosis and cell cycle of hl-60 were detected by tunel staining and flow cytometry with annexin v-fitc/pi double labeling, the expression of pakt and cyclin d1 at protein and gene level in hl-60 cells treated with celastrol were measured by western blot and rt-pcr. |
2011-09-30 |
2023-08-12 |
Not clear |
| Xiao-Hua Li, Tian-Yang Xue, Wei Xu, Ji-Zhao Ga. [Effect of proteasome inhibitor MG-132 on L1210 cell apoptosis and its mechanism]. Zhongguo shi yan xue ye xue za zhi. vol 18. issue 2. 2011-09-29. PMID:20416160. |
cell viability was tested by mtt assay, apoptosis rate was detected by using flow cytometry, activity of caspase 3 was detected by colorimetry, the expression of nf-kappab nuclear protein was detected by western blot. |
2011-09-29 |
2023-08-12 |
Not clear |
| Hairong Cheng, Shanshan Li, Yuying Fan, Xiaoge Gao, Miao Hao, Jia Wang, Xiaoyan Zhang, Guihua Tai, Yifa Zho. Comparative studies of the antiproliferative effects of ginseng polysaccharides on HT-29 human colon cancer cells. Medical oncology (Northwood, London, England). vol 28. issue 1. 2011-08-02. PMID:20165990. |
ginseng polysaccharide fractions and their temperature-modified products were assayed for their effects on ht-29 cell proliferation by mtt assay, on cell cycle progression by flow cytometry, and on caspase-3 activation by western blot analysis. |
2011-08-02 |
2023-08-12 |
human |
| Ping Liu, Lei Zhao, Shu-Ling Zhang, Ji-Zhou Xian. Modified Wendan Decoction can Attenuate Neurotoxic Action Associated with Alzheimer's Disease. Evidence-based complementary and alternative medicine : eCAM. vol 6. issue 3. 2011-07-14. PMID:18955245. |
cell mortality and survival rate was examined by trypan blue stain assay and mtt method and caspase-3 expression was detected by western blot, while cell apoptosis was examined by flow cytometry. |
2011-07-14 |
2023-08-12 |
rat |
| Siddig Ibrahim Abdelwahab, Syam Mohan, Manal Mohamed Elhassan, Nabil Al-Mekhlafi, Abdelbasit Adam Mariod, Ahmad Bustamam Abdul, Mahmood Ameen Abdulla, Khalid M Alkharf. Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway. Evidence-based complementary and alternative medicine : eCAM. vol 2011. 2011-07-14. PMID:21234328. |
the role of os against hydrogen peroxide induced apoptosis on mda-m231 epithelial cells was examined using mtt assay, phase contrast microscope, colorimetric assay of caspase-3, western blot and quantitative real-time pcr. |
2011-07-14 |
2023-08-12 |
cat |
| Zhen-Chuan Shang, Ting-Ting Song, Yang-Tang Hou, Li Fu, Tao Deng, Hai Yi, Tao Zhang, Bing-Zhong Su. [Role of mitochondria pathway in signal transduction of chronic myeloid leukemia]. Zhongguo shi yan xue ye xue za zhi. vol 17. issue 2. 2011-07-12. PMID:19379550. |
after bcr3/abl2 antisense oligodeoxynucleotide (aso) was introduced into cml cell line k562 cells by liposomal transfection, the cell viability was detected by mtt assay, the cell apoptosis was determined by flow cytometry (fcm), the mitochondrial membrane potential (deltapsi) was labeled by rhodamine 123 and examined by fcm, and the expression of mitochondrial apoptosis signal transduction pathway related proteins cytochrome c was analyzed by western blot. |
2011-07-12 |
2023-08-12 |
Not clear |
| Zhiwei Chen, Xiaomin Niu, Ziming Li, Yongfeng Yu, Xiangyun Ye, Shun Lu, Zhen Che. Effect of ubiquitin carboxy-terminal hydrolase 37 on apoptotic in A549 cells. Cell biochemistry and function. vol 29. issue 2. 2011-06-20. PMID:21287580. |
using mtt assay, western blot, hoechst 33342 staining assay and flow cytometry, we found that silencing of uch37 in a549 cells induced apoptosis. |
2011-06-20 |
2023-08-12 |
Not clear |
| Min Hao, Shu-yuan Li, Chang-kai Sun, Jingyu-Xu, Yuan Lin, Ke-xin Liu, Li Wang, Chuan-xun Li, Qin Zhou, Jian-ling Du, Hua L. Amelioration effects of berberine on diabetic microendothelial injury model by the combination of high glucose and advanced glycation end products in vitro. European journal of pharmacology. vol 654. issue 3. 2011-05-31. PMID:21236251. |
we prepared ages, established the high glucose-ages injured microec models by mtt assay, which was further supported by significantly decreased nitric oxide (no) release, no synthase (nos) and thrombomodulin production with elisa, western blot and rt-pcr analysis. |
2011-05-31 |
2023-08-12 |
Not clear |
| Eduardo Parra, Jorge Ferreir. Knockdown of the c-Jun-N-terminal kinase expression by siRNA inhibits MCF-7 breast carcinoma cell line growth. Oncology reports. vol 24. issue 5. 2011-02-18. PMID:20878129. |
we assessed the effects of jnk-1 or jnk-2 silencing on cell growth by western blot analysis, soft agar assay, cell proliferation assay, cell viability by mtt assay and caspase assay in vitro. |
2011-02-18 |
2023-08-12 |
human |
| Gabriella Aviello, Ian Rowland, Christopher I Gill, Angela Maria Acquaviva, Francesco Capasso, Mark McCann, Raffaele Capasso, Angelo A Izzo, Francesca Borrell. Anti-proliferative effect of rhein, an anthraquinone isolated from Cassia species, on Caco-2 human adenocarcinoma cells. Journal of cellular and molecular medicine. vol 14. issue 7. 2011-01-20. PMID:19538468. |
cytotoxicity studies were performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt), neutral red (nr) and trans-epithelial electrical resistance (teer) assays whereas (3)h-thymidine incorporation and western blot analysis were used to evaluate the effect of rhein on cell proliferation. |
2011-01-20 |
2023-08-12 |
human |
| Debby Reuveni, Drora Halperin, Itamar Shalit, Esther Priel, Ina Fabia. Moxifloxacin enhances etoposide-induced cytotoxic, apoptotic and anti-topoisomerase II effects in a human colon carcinoma cell line. International journal of oncology. vol 37. issue 2. 2010-11-09. PMID:20596674. |
decatenation assay, band depletion and western blot analysis, cytotoxic assay (mtt), flow cytometric studies (cell cycle and survivin expression), apoptosis (dapi-sulforhodamine staining and caspase 3 activity) and il-8 and vegf secretion were determined. |
2010-11-09 |
2023-08-12 |
human |
| Qin Hu, Ruile Pan, Liwei Wang, Bo Peng, Jingtian Tang, Xinmin Li. Platycodon grandiflorum induces apoptosis in SKOV3 human ovarian cancer cells through mitochondrial-dependent pathway. The American journal of Chinese medicine. vol 38. issue 2. 2010-07-29. PMID:20387232. |
3-(4,5- dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (mtt) assay was used to monitor cell numbers, annexin-v/propidium iodide (pi) staining, rt-pcr and western blot were used to examine cell apoptosis, caspases activation. |
2010-07-29 |
2023-08-12 |
human |
| Roberto E Favoni, Alessandra Pattarozzi, Michele Lo Casto, Federica Barbieri, Monica Gatti, Laura Paleari, Adriana Bajetto, Carola Porcile, Giovanni Gaudino, Luciano Mutti, Giorgio Corte, Tullio Flori. Gefitinib targets EGFR dimerization and ERK1/2 phosphorylation to inhibit pleural mesothelioma cell proliferation. Current cancer drug targets. vol 10. issue 2. 2010-07-22. PMID:20088784. |
cell proliferation, dna synthesis and apoptosis were measured by mtt, thymidine incorporation and facs analysis; egfr, erk1/2 and akt expression and phosphorylation by western blot, whereas receptor sites were analyzed by binding studies. |
2010-07-22 |
2023-08-12 |
human |
| Yunlan Li, Jinjie Liu, Qingshan L. Mechanisms by which the antitumor compound di-n-butyl-di-(4-chlorobenzohydroxamato)tin(IV) induces apoptosis and the mitochondrial-mediated signaling pathway in human cancer SGC-7901 cells. Molecular carcinogenesis. vol 49. issue 6. 2010-06-29. PMID:20336762. |
inhibition of proliferation of four cancer cell lines compared with normal human hepatic l-o2 cells, cancer cell apoptosis, and expression of related mrna and protein were detected using the methyl thiazolyl tetrazolium (mtt), flow cytometry, reverse transcription polymerase chain reaction (rt-pcr), western blot, and dna ladder assays, and electron microscopy and immunocytochemistry. |
2010-06-29 |
2023-08-12 |
human |
| Guo-Guang Wang, Min Xu, Hai-Feng Duan, Gen-Bao Zhan. [Apoptosis of K562 cells induced by extract of Agkistrodon Halys' venom]. Zhongguo shi yan xue ye xue za zhi. vol 16. issue 3. 2010-01-12. PMID:18549620. |
the inhibition of k562 cell proliferation was measured by mtt assay; the morphologic changes of k562 cells was observed by microscopy; the apoptosis of k562 cells was measured by flow cytometry; the activity of extracellular signal-regulated kinase (erk) in k562 cells was detected by western blot. |
2010-01-12 |
2023-08-12 |
Not clear |
| Jun-Ling Liu, Rong-Xi Yu, Jun-Fa Che. [Apoptosis of HL-60 cells induced by artesunate in vitro]. Zhongguo shi yan xue ye xue za zhi. vol 15. issue 3. 2009-12-11. PMID:17605854. |
the inhibition of art on hl-60 cells were evaluated by means of mtt assay; cell apoptosis was detected by light microscopy, agarose gel electro-phoresis, flow cytometry; western blot was used to analyze the expression of bcl-2 and icad in cells during apoptosis induced by art. |
2009-12-11 |
2023-08-12 |
Not clear |