| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Qi Qi, Pei Zhang, Qi-Xiang Li, Qiong Pan, Hai-Lun Zheng, Su-Rong Zha. [Effect of oridonin on apoptosis and intracellular reactive oxygen species level in triple-negative breast cancer MDA-MB-231 cells]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 42. issue 12. 2018-06-29. PMID:28822194. |
the inhibitory effect of oridonin on proliferation of mda-mb-231 cells was measured by mtt assay; apoptosis was analyzed by flow cytometry with pi staining and annexin v-fitc/pi staining; intracellular reactive oxygen species (ros) level was determined by ros detection kit, and expressions of parp, bcl-2, caspase-3 were analyzed by western blot. |
2018-06-29 |
2023-08-13 |
Not clear |
| Md Rashedunnabi Akanda, Byung-Yong Par. Involvement of MAPK/NF-κB signal transduction pathways: Camellia japonica mitigates inflammation and gastric ulcer. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 95. 2018-06-11. PMID:28926923. |
to evaluate the protective roles of camellia japonica on lps-induced inflammation in raw 264.7 cells and hcl/etoh-induced gastric ulcer in mice; we applied 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt), nitric oxide (no), reactive oxygen species (ros), histopathology, malondialdehyde (mda), quantitative real-time polymerase chain reaction (qpcr), immunohistochemistry (ihc), and western blot analyses. |
2018-06-11 |
2023-08-13 |
mouse |
| Weiwei Zhang, Ying Li, Peng Wan. Long non-coding RNA-ROR aggravates myocardial ischemia/reperfusion injury. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. vol 51. issue 6. 2018-05-29. PMID:29694511. |
cell viability, apoptosis, apoptosis-associated factors, and p38/mapk pathway were examined by mtt, flow cytometry, and western blot assays. |
2018-05-29 |
2023-08-13 |
human |
| Feifan Xu, Yuanlong Han, Dandan Zhu, Hua Tian, Huiming Zhu, Jingjing Ren, Delin Gu, Yinong Dua. Construction of a recombinant pIRES2-EGFP-ARTS plasmid and its effect on LX-2 cells. Molecular medicine reports. vol 16. issue 4. 2018-05-21. PMID:28791356. |
in addition, the effects of pires2‑egfp‑arts on the activation, apoptosis, viability and migration of lx‑2 cells were assessed by western blot analysis, tunel staining, an mtt assay, and scratch and transwell assays, respectively. |
2018-05-21 |
2023-08-13 |
human |
| Qiang Wan, Yu-Ping Yang, Zhong-Yong Li. [Puerarin attenuates PM2.5-induced vascular endothelial cells injury via ERK1/2 signaling pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 41. issue 12. 2018-05-18. PMID:28901078. |
different concentrations of pm2.5 (0,20, 200, 400 mg•l⁻¹) were used to contaminate ea.hy926 cells for 24 h. the cells survival rate was detected by mtt assay; cells apoptosis of ea.hy926cells was detected by flow cytometry; the protein levels of p-erk1/2, bax and bcl-2 were detected by western blot; the contents of tumor necrosis factor-α (tnf-α), interleukin-6 (il-6), malonaldehyde (mda), and the activities of superoxide dismutase (sod) and lactic dehydrogenase (ldh) were measured by elisa. |
2018-05-18 |
2023-08-13 |
human |
| Jong Seok Lee, Jun Myoung Park, Sarah Lee, Hye Jin Lee, Hee-Sun Yang, Joohong Yeo, Ki Rim Lee, Byung Hyun Choi, Eock Kee Hon. Hispidin rescues palmitate‑induced insulin resistance in C2C12 myotubes. Molecular medicine reports. vol 16. issue 4. 2018-05-14. PMID:28731188. |
the present study investigated the effects of hispidin on palmitate‑induced insulin resistance in c2c12 skeletal muscle myotubes via an mtt assay, glucose uptake assay, oil‑red‑o staining and western blot analysis. |
2018-05-14 |
2023-08-13 |
Not clear |
| Dong Zhao, Jinhua Jia, Hong Sha. miR-30e targets GLIPR-2 to modulate diabetic nephropathy: Journal of molecular endocrinology. vol 59. issue 2. 2018-05-10. PMID:28733476. |
mtt and flow cytometry were utilized to measure the proliferation and apoptosis of rtecs, while qrt-pcr and western blot to detect the expression of glipr-2- and emt-related factors. |
2018-05-10 |
2023-08-13 |
mouse |
| Nadiar Mussin, Seung Cheol Oh, Kwang Woong Lee, Min Young Park, Sooin Seo, Nam Joon Yi, Hyeyoung Kim, Kyung Chul Yoon, Sung Woo Ahn, Hyo Sin Kim, Suk Kyun Hong, Dong Kyu Oh, Kyung Suk Su. Sirolimus and Metformin Synergistically Inhibits Colon Cancer In Vitro and In Vivo. Journal of Korean medical science. vol 32. issue 9. 2018-04-30. PMID:28776332. |
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay was performed and western blot analyses were performed for mtor pathway proteins, apoptosis proteins, and epithelial-mesenchymal-transition (emt) proteins. |
2018-04-30 |
2023-08-13 |
mouse |
| Wenlei Li, Guotao Jia, Yanwen Qu, Qian Du, Baoguo Liu, Bin Li. Long Non-Coding RNA (LncRNA) HOXA11-AS Promotes Breast Cancer Invasion and Metastasis by Regulating Epithelial-Mesenchymal Transition. Medical science monitor : international medical journal of experimental and clinical research. vol 23. 2018-04-23. PMID:28701685. |
material and methods in this study, fluorescent quantitative real-time pcr (qrt-pcr), mtt assay and clone formation assay, flow cytometry, transwell assay and wound healing assay, immunofluorescence, and western blot analysis were conducted to detect the expression of lncrna hoxa11-as, cell proliferation activity, cell apoptosis rate and cell cycle distribution, the changes of cell invasion and metastasis capacity, and the expressions of molecular markers of epithelial-mesenchymal transition (emt), respectively. |
2018-04-23 |
2023-08-13 |
mouse |
| Fenghui Chen, Lixin Zhang, Erwei Wang, Chaofeng Zhang, Xiaoting L. LncRNA GAS5 regulates ischemic stroke as a competing endogenous RNA for miR-137 to regulate the Notch1 signaling pathway. Biochemical and biophysical research communications. vol 496. issue 1. 2018-02-15. PMID:29307821. |
the mechanism of gas5 in ischemic stroke was evaluated both in vivo and in vitro via bioinformatics analysis, mtt, flow cytometry, luciferase assay, rna immunoprecipitation, and western blot. |
2018-02-15 |
2023-08-13 |
mouse |
| Yun Li, Wei Sun, Song Han, Jianing Li, Shu Ding, Wei Wang, Yanling Yi. IGF-1-Involved Negative Feedback of NR2B NMDA Subunits Protects Cultured Hippocampal Neurons Against NMDA-Induced Excitotoxicity. Molecular neurobiology. vol 54. issue 1. 2018-02-08. PMID:26758454. |
in this study, we observed that igf-1 can salvage the hippocampal neurons in an autophagy-independent manner after 150-μm nmda exposure using thiazolyl blue tetrazolium bromide (mtt), lactate dehydrogenase (ldh), western blot assay, and transmission electron microscopy. |
2018-02-08 |
2023-08-13 |
Not clear |
| Raffaele Pezzani, Beatrice Rubin, Loris Bertazza, Marco Redaelli, Susi Barollo, Halenya Monticelli, Enke Baldini, Caterina Mian, Carla Mucignat, Carla Scaroni, Franco Mantero, Salvatore Ulisse, Maurizio Iacobone, Marco Boscar. The aurora kinase inhibitor VX-680 shows anti-cancer effects in primary metastatic cells and the SW13 cell line. Investigational new drugs. vol 34. issue 5. 2017-12-14. PMID:27177645. |
biomolecular and cellular tests were performed (such as mtt, thymidine assay, wright's staining, cell cycle and apoptosis analysis, western blot, qrt-pcr, and mutation analysis). |
2017-12-14 |
2023-08-13 |
Not clear |
| Xia Chen, Dan Peng, Yi Shen, Bo Liu, Hongkang Zhou, Huai Tao, Jufang Huan. The potential combinational effect of miR-34a with celecoxib in osteosarcoma. Anti-cancer drugs. vol 28. issue 8. 2017-12-06. PMID:28650862. |
in this study, the effects of cel, mir-34a mimics, and their combination on cell proliferation (mtt assay), migration (in-vitro scratch assay), invasion (transwell assay), mrna (real-time pcr), and protein (western blot) expression of associated signal transductions were investigated in cultured mg63 cells. |
2017-12-06 |
2023-08-13 |
Not clear |
| Eduarda Lopes-Costa, Mariana Abreu, Daniela Gargiulo, Eduardo Rocha, Alice A Ramo. Anticancer effects of seaweed compounds fucoxanthin and phloroglucinol, alone and in combination with 5-fluorouracil in colon cells. Journal of toxicology and environmental health. Part A. vol 80. issue 13-15. 2017-10-19. PMID:28850007. |
effects of these compounds on cell viability, induction of dna damage, and cell death were evaluated using mtt assay, comet assay, nuclear condensation assay, and western blot. |
2017-10-19 |
2023-08-13 |
human |
| Lifeng Wang, Yingjie Lu, Xian Liu, Xiaoyun Wan. Ghrelin protected neonatal rat cardiomyocyte against hypoxia/reoxygenation injury by inhibiting apoptosis through Akt-mTOR signal. Molecular biology reports. vol 44. issue 2. 2017-10-10. PMID:28281036. |
we isolated the nrcms, established myocardial h/r model, blocked growth hormone secretagogue receptor (ghsr) by sirna technique, examined cell activity by mtt and ldh assay, detected apoptosis by hoechst 33258 staining and flow cytometry and determined the expression levels of apoptosis related proteins and signaling pathway proteins by western blot. |
2017-10-10 |
2023-08-13 |
rat |
| Q D Mao, W Zhang, K Zhao, B Cao, H Yuan, L Z Wei, M Q Song, X S Li. MicroRNA-455 suppresses the oncogenic function of HDAC2 in human colorectal cancer. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. vol 50. issue 6. 2017-09-25. PMID:28538837. |
finally, cells were transfected, respectively, with mir-455 mimics or mir-455 negative control (mir-nc) and the expression of hdac2, cell proliferation and apoptosis of transfected cells were respectively evaluated by western blot analysis, mtt assay and flow cytometry. |
2017-09-25 |
2023-08-13 |
human |
| X-G Li, W-G Sui, C-Q Gao, H-C Yan, Y-L Yin, H-C Li, X-Q Wan. L-Glutamate deficiency can trigger proliferation inhibition via down regulation of the mTOR/S6K1 pathway in pig intestinal epithelial cells. Journal of animal science. vol 94. issue 4. 2017-05-31. PMID:27136013. |
cell proliferation ( = 24), cell cycle distribution ( = 6), cell apoptosis ( = 6), and expression levels of proteins of interest ( = 4) were determined by mtt assay, flow cytometry, or western blot. |
2017-05-31 |
2023-08-13 |
Not clear |
| D Bai, S Cong, L P Zh. Attenuation of Focal Adhesion Kinase Reduces Lipopolysaccharide-Induced Inflammation Injury through Inactivation of the Wnt and NF-κB Pathways in A549 Cells. Biochemistry. Biokhimiia. vol 82. issue 4. 2017-05-31. PMID:28371601. |
flow cytometry, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, quantitative reverse transcription polymerase chain reaction, and western blot analysis were used to explore the correlation of fak expression with cell apoptosis, viability, and the inflammatory cytokine activity in a549 cells. |
2017-05-31 |
2023-08-13 |
Not clear |
| Ning Yang, Yuying Zhao, Zhipeng Wang, Yu Liu, Yanqiao Zhan. Scutellarin suppresses growth and causes apoptosis of human colorectal cancer cells by regulating the p53 pathway. Molecular medicine reports. vol 15. issue 2. 2017-05-16. PMID:28035355. |
human colon cancer cells were exposed to different concentrations of scutellarin, and cellular growth and apoptosis were evaluated by mtt assay, terminal deoxynucleotidyl transferase‑mediated dutp nick end labeling (tunel) staining, western blot analysis and other assays. |
2017-05-16 |
2023-08-13 |
human |
| Francesca Uberti, Claudio Bardelli, Vera Morsanuto, Sabrina Ghirlanda, Andrea Cochis, Claudio Molinar. Stimulation of the Nonneuronal Cholinergic System by Highly Diluted Acetylcholine in Keratinocytes. Cells, tissues, organs. vol 203. issue 4. 2017-05-15. PMID:27883993. |
two different concentrations (10 fg/ml and 1 pg/ml) and formulations (kinetically activated and nonkinetically activated) of acetylcholine were used to verify keratinocyte viability, proliferation, and migration and the intracellular pathways involved using mtt, crystal violet, wound healing, and western blot compared to 147 ng/ml acetylcholine. |
2017-05-15 |
2023-08-13 |
human |