| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Yi Zhang, Li Zhang, Qiyu Zhang, Weilong Hong, Xiaohua Li. [microRNA-222 regulates proliferation and apoptosis of fibroblasts in hypertrophic scar via matrix metalloproteinase 1]. Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences. vol 46. issue 6. 2019-05-20. PMID:29658663. |
the cell viability was tested with mtt assay, cell cycle distribution and apoptosis were detected with flow cytometry and the expression levels of proliferation, apoptosis and cell cycle related proteins were determined with western blot. |
2019-05-20 |
2023-08-13 |
Not clear |
| Haibin Yan, Bingyun Zhang, Chongbin Fang, Liqiu Che. miR-340 alleviates chemoresistance of osteosarcoma cells by targeting ZEB1. Anti-cancer drugs. vol 29. issue 5. 2019-05-08. PMID:29494357. |
plasmid construction and transfection, mirna arrays, pcr analyses, and western blot analysis, as well as mtt, apoptosis, and luciferase assays were carried out in mg-63 cells and mg-63/cisplatin (ddp)-resistant cells. |
2019-05-08 |
2023-08-13 |
Not clear |
| Ke-Feng Zhai, Hong Duan, Cai-Yue Cui, Yu-Yao Cao, Jia-Li Si, Hui-Jiao Yang, Yong-Chao Wang, Wen-Gen Cao, Gui-Zhen Gao, Zhao-Jun We. Liquiritin from Glycyrrhiza uralensis Attenuating Rheumatoid Arthritis via Reducing Inflammation, Suppressing Angiogenesis, and Inhibiting MAPK Signaling Pathway. Journal of agricultural and food chemistry. vol 67. issue 10. 2019-03-22. PMID:30785275. |
an mtt assay, hoechst 33342 staining, jc-1 staining, and western blot were used to check the viability, cell apoptosis, mitochondrial membrane potential changes, and the expression of related proteins, respectively. |
2019-03-22 |
2023-08-13 |
Not clear |
| Hao Wu, Chao Zeng, Yiwang Ye, Jixian Liu, Zhimin Mu, Yuancai Xie, Baokun Chen, Qiaohong Nong, Da W. Exosomes from Irradiated Nonsmall Cell Lung Cancer Cells Reduced Sensitivity of Recipient Cells to Anaplastic Lymphoma Kinase Inhibitors. Molecular pharmaceutics. vol 15. issue 5. 2019-03-18. PMID:29595984. |
western blot, flow cytometry, mtt, and xenograft were employed to respectively assess activation of the alk pathway, apoptosis, cell viability, and tumor growth. |
2019-03-18 |
2023-08-13 |
mouse |
| Zhengpeng Zeng, Shenghua Sun, Lihua Xi. [Effect of miR-21 on autophagy, proliferation and apoptosis of macrophages induced by cigarette smoke extract]. Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences. vol 43. issue 12. 2019-02-11. PMID:30643042. |
the effects of mir-21 inhibitor on macrophage autophagy, proliferation and apoptosis were detected by western blot, mtt assay and flow cytometry.
results: compared with the control group, the levels of mir-21 and autophagy in the cse group were significantly increased (both p<0.05). |
2019-02-11 |
2023-08-13 |
Not clear |
| Wanyuan Chen, Weimin Fan, Guoqing Ru, Fang Huang, Xiaming Lu, Xin Zhang, Xiaozhou Mou, Shibing Wan. Gemcitabine combined with an engineered oncolytic vaccinia virus exhibits a synergistic suppressive effect on the tumor growth of pancreatic cancer. Oncology reports. vol 41. issue 1. 2019-01-22. PMID:30365143. |
the expression of smac was evaluated by western blot analysis and quantitative polymerase chain reaction, ovv‑smac cytotoxicity by mtt assay, and apoptosis by flow cytometry and western blot analysis. |
2019-01-22 |
2023-08-13 |
Not clear |
| Bing-Gang Zhou, Chang-Sheng Wei, Song Zhang, Zhi Zhang, Huan-Min Ga. Matrine reversed multidrug resistance of breast cancer MCF-7/ADR cells through PI3K/AKT signaling pathway. Journal of cellular biochemistry. vol 119. issue 5. 2018-12-11. PMID:29130495. |
methyl thiazolyl tetrazolium (mtt) assay was used to detect the inhibitory action; annexin v to detect apoptosis; fluorospectrophotometry to examine intracellular adriamycin (adr) accumulation; and western blot to label the proteins of p-glycoprotein (p-gp), mrp1, pten, p-akt, bcl-2, bax, and caspase-3. |
2018-12-11 |
2023-08-13 |
Not clear |
| Yuexia Liao, Yang Xu, Mengyao Cao, Yuanyuan Huan, Lei Zhu, Ye Jiang, Weigan Shen, Guoqiang Zh. Luteolin Induces Apoptosis and Autophagy in Mouse Macrophage ANA-1 Cells via the Bcl-2 Pathway. Journal of immunology research. vol 2018. 2018-12-11. PMID:30246034. |
the viability of the cells was determined by an mtt assay, apoptosis was determined by flow cytometric analysis, the level of cell autophagy was observed by confocal microscopy, and the expression levels of apoptotic or autophagic and antiapoptotic or antiautophagic proteins were detected by western blot analysis. |
2018-12-11 |
2023-08-13 |
mouse |
| Wei Han, Fang Cao, Xiao-Jiao Gao, Hua-Bing Wang, Fang Chen, Si-Jian Cai, Cong Zhang, Yong-Wei Hu, Jun Ma, Xing Gu, Hou-Zhong Din. ZIC1 acts a tumor suppressor in breast cancer by targeting survivin. International journal of oncology. vol 53. issue 3. 2018-11-26. PMID:29956756. |
subsequently, the mda-mb-231 and sk-br3 cells with a lower zic1 expression were transfected with rlv-zic1-pgk-puro lentivirus or rlv-zsgreen-pgk‑puro lentivirus in order to observe any alterations in cell proliferation and apoptosis through mtt assay, colony formation assay, mitochondrial membrane potential assay and flow cytometric analysis, and to analyze the modulation of molecular mechanisms by western blot analysis. |
2018-11-26 |
2023-08-13 |
mouse |
| Chuan Lin, Yu Qin, Hua Zhang, Ming-Yang Gao, Yan-Fu Wan. EGF upregulates RFPL3 and hTERT via the MEK signaling pathway in non‑small cell lung cancer cells. Oncology reports. vol 40. issue 1. 2018-10-11. PMID:29749533. |
expression of rfpl3 and htert at the mrna and protein levels was determined by real‑time quantitative pcr (rt‑qpcr) and western blot analysis; cell viability was detected by mtt assay, and apoptosis was assayed via flow cytometry. |
2018-10-11 |
2023-08-13 |
human |
| Xiaoli Li, Jian Liu, Li Qian, Honggang Ke, Chan Yao, Wei Tian, Yifei Liu, Jianguo Zhan. Expression of PFKFB3 and Ki67 in lung adenocarcinomas and targeting PFKFB3 as a therapeutic strategy. Molecular and cellular biochemistry. vol 445. issue 1-2. 2018-09-17. PMID:29327288. |
cell viability, cell cycle, cell apoptosis, cell migration, and invasion were analyzed by mtt, flow cytometry, western blot analysis, wound healing assay, and transwell chamber assay. |
2018-09-17 |
2023-08-13 |
human |
| Wei Shi, Xiaoling Wang, Lihong Ruan, Jiamei Fu, Fang Liu, Jinfeng Q. MiR-200a promotes epithelial-mesenchymal transition of endometrial cancer cells by negatively regulating FOXA2 expression. Die Pharmazie. vol 72. issue 11. 2018-08-29. PMID:29442045. |
mtt assay measured cell proliferation, apoptosis assay measured apoptosis, transwell invasion assay measured cell invasion, and western blot measured the protein expression of foxa2, e-cadherin, and vimentin. |
2018-08-29 |
2023-08-13 |
Not clear |
| Yan Feng, Li-Li Zu, Lei Zhan. MicroRNA-26b inhibits the tumor growth of human liver cancer through the PI3K/Akt and NF-κB/MMP-9/VEGF pathways. Oncology reports. vol 39. issue 5. 2018-08-29. PMID:29498407. |
forced upregulation of microrna-26b had anticancer effects, inhibited cell proliferation, induced apoptosis and promoted bax and caspase-3/-9 protein expression in a liver cancer cell line as determined by mtt assay, flow cytometry and western blot analysis. |
2018-08-29 |
2023-08-13 |
human |
| Lixue Zou, Jun Liu, Hougen L. Influence of protein kinase RIPK4 expression on the apoptosis and proliferation of chondrocytes in osteoarthritis. Molecular medicine reports. vol 17. issue 2. 2018-08-20. PMID:29257245. |
chondrocytes proliferation was detected by mtt assay, cell apoptosis was indicated using flow cytometry and wnt/β‑catenin signaling pathway related‑proteins were investigated using western blot analysis. |
2018-08-20 |
2023-08-13 |
Not clear |
| Shan-shan Meng, Guan Gui, Lu-juan Li, Bin Liu, Hong-xia Liang, Liang-ce Liu, Chao-shen Huangf. [Exposure of human hepatoma cells to nitrite and ammonia promotes invasive activity through activation of ROS/ODC pathway]. Yao xue xue bao = Acta pharmaceutica Sinica. vol 51. issue 7. 2018-08-20. PMID:29897188. |
smmc-7721 cells were treated with sodium nitrite, ammonium chloride, sodium nitrite and ammonium chloride mixture for 24 h, the cell viability was analyzed using the mtt assay, cell invasion was analyzed with the transwell assay, the intracellular ros levels were detected with a reactive oxygen species (ros) test kits, the expression of intracellular odc was examined with immunofluorescence and western blot, the expression of matrix metallopeptidase-2 (mmp-2) and mmp-9 were analyzed by western blot. |
2018-08-20 |
2023-08-13 |
human |
| Ou-Gen Liu, Xiao-Yan Xiong, Chun-Ming Li, Xian-Sheng Zhou, Si-Si L. Role of Xeroderma Pigmentosum Group D in Cell Cycle and Apoptosis in Cutaneous Squamous Cell Carcinoma A431 Cells. Medical science monitor : international medical journal of experimental and clinical research. vol 24. 2018-08-13. PMID:29362353. |
material and methods to explore the role of xpd in a431 cells, we overexpressed xpd in a431 cells and performed mtt assay, flow cytometry, and western blot analysis to examine cell proliferation, cell apoptosis, and genes expression. |
2018-08-13 |
2023-08-13 |
Not clear |
| Dayu Li, Yun Liu, Chunbo Yu, Xiping Liu, Fang Fa. [Knockdown of DNA-PKcs inhibits cell cycle and its mechanism of drug-resistant Bel7402/5-Fu hepatocellular carcinoma cells]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 33. issue 12. 2018-08-09. PMID:29382425. |
methods after cationic liposome-mediated sidna-pkcs oligonucleotide transfection, the drug sensitivity of bel7402/5-fu cells to 5-fluorouracil (5-fu) and adriamycin (adm) was determined by mtt assay; the cell cycle were detected by flow cytometry; meanwhile, the protein expressions of cell cycle-related proteins p21, cell cycle protein b1 (cyclin b1), cell cycle division protein 2 (cdc2) were tested by western blotting; the expressions of ataxia telangiectasia mutated (atm) and p53 at both mrna and protein levels were detected by real-time pcr and western blot analysis. |
2018-08-09 |
2023-08-13 |
Not clear |
| Qiang Liu, Hui Yang, Shengnan Xu, Xuemei Su. Downregulation of p300 alleviates LPS-induced inflammatory injuries through regulation of RhoA/ROCK/NF-κB pathways in A549 cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 97. 2018-08-03. PMID:29091886. |
mtt, flow cytometry, qrt-pcr and western blot assays were used to assess the effect of p300 on a549 cells viability, apoptosis and inflammatory cytokines expressions. |
2018-08-03 |
2023-08-13 |
Not clear |
| Zhenkai Lou, Xingguo Li, Xueling Zhao, Kaili Du, Xing Li, Bing Wan. Resveratrol attenuates hydrogen peroxide‑induced apoptosis, reactive oxygen species generation, and PSGL‑1 and VWF activation in human umbilical vein endothelial cells, potentially via MAPK signalling pathways. Molecular medicine reports. vol 17. issue 2. 2018-07-18. PMID:29207192. |
huvecs were pretreated with resveratrol for 2 h and incubated with hydrogen peroxide (h2o2) for 24 h prior to the evaluation of cell viability, ros generation, apoptosis and thrombosis‑associated marker expression by performing mtt assays, 2',7'‑dichlorofluorescin diacetate reagent, flow cytometry, and reverse transcription‑quantitative polymerase chain reaction (rt‑qpcr) and western blot analysis, respectively. |
2018-07-18 |
2023-08-13 |
human |
| Shuke Ge, Dan Wang, Qinglong Kong, Wei Gao, Jiayi Su. Function of miR-152 as a Tumor Suppressor in Human Breast Cancer by Targeting PIK3CA. Oncology research. vol 25. issue 8. 2018-07-02. PMID:28247844. |
the efficiency of mir-152-3p transfection was evaluated by quantitative real-time pcr, and the effects on cell viability and apoptosis as well as on the pi3k/akt signaling pathway were investigated by mtt assay, flow cytometry, and western blot analysis, respectively. |
2018-07-02 |
2023-08-13 |
human |