| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Dengtao Liu, Ping Han, Chunhai Gao, Wei Gao, Xiaocui Yao, Shulan Li. microRNA-155 Modulates Hepatic Stellate Cell Proliferation, Apoptosis, and Cell Cycle Progression in Rats With Alcoholic Hepatitis Frontiers in pharmacology. vol 11. 2020-09-28. PMID:32317960. |
the biological prediction website microrna.org and dual-luciferase reporter gene assay were used to identify whether socs1 was a direct target of mir-155, and the effects of mir-155 and socs1 on the viability, cycle progression, and apoptosis of hepatic stellate cells were assessed using rt-qpcr, western blot assay, mtt assay, annexin v/pi double staining, and pi single staining. |
2020-09-28 |
2023-08-13 |
rat |
| Xiaozhi Deng, Lihong Shang, Min Du, Lan Yuan, Lijing Xiong, Xiaoli Xi. Mechanism underlying the significant role of the miR-4262/SIRT1 axis in children with inflammatory bowel disease. Experimental and therapeutic medicine. vol 20. issue 3. 2020-09-28. PMID:32765699. |
mtt assay, flow cytometry and western blot analysis were performed to assess cell viability, apoptosis and nf-κb signaling pathway-related protein levels, respectively. |
2020-09-28 |
2023-08-13 |
Not clear |
| Shefeng Zheng, Jinnv Liu, Zhangjian Zhao, Ruifang Son. Role of STAT3/mTOR pathway in chronic kidney injury. American journal of translational research. vol 12. issue 7. 2020-09-28. PMID:32774701. |
glomerular mesangial cells were divided into control group, model group, stat3 inhibitor (s3i-201) group followed by analysis of cell proliferation by mtt assay, cell apoptosis by flow cytometry, formation of autophagosomes by electron microscopy, expression of stat3/mtor signaling proteins and autophagy proteins lc3ii and p62 by western blot, expression of e-cadherin and vimentin by immunofluorescence. |
2020-09-28 |
2023-08-13 |
mouse |
| Ben Wang, Sha Yan, Yuxin Yi, Yingxue Huang, Zhili Deng, Yiya Zhang, Qingchuan Zheng, Hongfu Xie, Ji L. Purified Vitexin Compound 1 Inhibits UVA-Induced Cellular Senescence in Human Dermal Fibroblasts by Binding Mitogen-Activated Protein Kinase 1. Frontiers in cell and developmental biology. vol 8. 2020-09-28. PMID:32850814. |
here, we demonstrated that vb1 significantly attenuates ultraviolet a (uva)-induced senescence in human dermal fibroblasts (hdfs), as evidenced by senescence-associated β-gal staining, mtt assays, and western blot analysis of the expression of p16 and matrix metalloproteinase-1 (mmp-1). |
2020-09-28 |
2023-08-13 |
mouse |
| Bo Zhang, Haijun Zhang, Hao Luo, Chao Yang, Yi Yua. Sinomenine can promote the proliferation and differentiation of osteoblasts by regulating the Akt/Runx2 signaling pathway in MC3T3-E1 cells. Die Pharmazie. vol 74. issue 12. 2020-09-23. PMID:31907116. |
the proliferation and apoptosis of the cells were determined by mtt assay at 0, 24, 48 and 72 h, and flow cytometry method was used to determine the effect of sinomenine on the apoptosis of mc3t3-e1 cells at 72 h; furthermore, after 72 h, the cell culture supernatants were collected, and the levels of alkaline phosphatase and osteocalcin were examined by enzyme-linked immunosorbent assay (elisa); next, cells were collected, and the expression of type i collagen (col1a1), osteopontin (opn), protein kinase b (akt), runt-related transcription factor 2 (runx2), b-cell lymphoma 2 (bcl-2) and bcl-2-associated x protein (bax) were examined by rt-qpcr and western blot methods. |
2020-09-23 |
2023-08-13 |
mouse |
| Jiao Yue, Xin-Shang Wang, Bin Feng, Li-Ning Hu, Liu-Kun Yang, Liang Lu, Kun Zhang, Ya-Tao Wang, Shui-Bing Li. Activation of G-Protein-Coupled Receptor 30 Protects Neurons against Excitotoxicity through Inhibiting Excessive Autophagy Induced by Glutamate. ACS chemical neuroscience. vol 10. issue 10. 2020-09-04. PMID:31545891. |
in this study, methyl thiazolyl tetrazolium (mtt), western blot, monodansylcadaverine (mdc) staining, and immunofluorescent staining were employed to detect the role of autophagy in cultured primary cortical neurons after glutamate exposure and g1 treatment. |
2020-09-04 |
2023-08-13 |
Not clear |
| Daniela Carneiro de Lima, Silvana Vianna Rodrigues, Gilson Teles Boaventura, Hee-Yeon Cho, Thomas C Chen, Axel H Schönthal, Clovis Orlando Da Fonsec. Simultaneous measurement of perillyl alcohol and its metabolite perillic acid in plasma and lung after inhalational administration in Wistar rats. Drug testing and analysis. vol 12. issue 2. 2020-09-01. PMID:31800149. |
toxicity was established by a standard mtt assay, and apoptosis markers were analyzed by western blot. |
2020-09-01 |
2023-08-13 |
rat |
| Sadaf Ghanaatgar-Kasbi, Forouzan Amerizadeh, Farzad Rahmani, Seyed Mahdi Hassanian, Majid Khazaei, Gordon A Ferns, Amir Ava. AMP-kinase inhibitor dorsomorphin reduces the proliferation and migration behavior of colorectal cancer cells by targeting the AKT/mTOR pathway. IUBMB life. vol 71. issue 12. 2020-07-07. PMID:31359579. |
the antiproliferative, antimigratory, apoptotic activity and effect of this agent on cell cycle was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, wound healing assay, and flow cytometry, respectively, while the expression of genes involved in wnt/pi3k pathways was assessed at mrna and/or proteins by reverse transcription polymerase chain reaction (rt-pcr) or western blot. |
2020-07-07 |
2023-08-13 |
Not clear |
| X Qin, X Y Wang, J W Fei, F H Li, J Han, H X Wan. MiR-20a promotes lung tumorigenesis by targeting RUNX3 via TGF-β signaling pathway. Journal of biological regulators and homeostatic agents. vol 34. issue 2. 2020-04-29. PMID:32347076. |
the analysis of cell growth and apoptosis were performed by mtt, xenograft models, western blot assays. |
2020-04-29 |
2023-08-13 |
human |
| Zi-Jian Yang, Jun-Yao Huang, Ye-Fei Gao, Jun-Yao Huang, Wen-Ji Huang, Jun-Yao Huang, Shi-Lei Xu, Jun-Yao Huang, Jing-Jing Jin, Jun-Yao Huang, Ye-Dong Wan, Jun-Yao Huang, Ming Yan, Jun-Yao Huang, Hong-Jiao Mao, Jun-Yao Huang, Yun Zhang, Jun-Yao Huan. [Effects of bergapten on damages of osteocytes MLO-Y4 induced by TCP wear particles and its mechanism]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology. vol 35. issue 6. 2020-04-14. PMID:32239867. |
mtt assay and calcein-am staining were used to determine the viability of mlo-y4 cells; hoechst 33342 staining and the flow cytometry were applied to detect the apoptosis of mlo-y4; real-time pcr was performed to examine the mrna levels of dentin matrix protein1 (dmp-1), sclerostin (sost) and fibroblast growth factor23 (fgf23); western blot was performed to examine protein expressions of glucose-regulated protein 78 (grp78), protein kinase r-like er kinase (perk) phospho-perk (p-perk), eukaryotic initiation factor 2α (eif2α), phospho-eif2α (p-eif2α), activating transcription factor 4 (aft4), c/ebp homologous protein (chop) and caspase-3 in mlo-y4 cells. |
2020-04-14 |
2023-08-13 |
Not clear |
| Zhiyuan Zhu, Pingde Zhang, Ning Li, Karrie Mei Yee Kiang, Stephen Yin Cheng, Vincent Kam-Wai Wong, Gilberto Ka-Kit Leun. Lovastatin Enhances Cytotoxicity of Temozolomide via Impairing Autophagic Flux in Glioblastoma Cells. BioMed research international. vol 2019. 2020-03-25. PMID:31662972. |
cytotoxicity was measured by mtt and colony formation assays; apoptosis was measured by flow cytometry; the cellular autophagic function was detected by the egfp-mrfp-lc3 reporter and western blot assay. |
2020-03-25 |
2023-08-13 |
Not clear |
| Jinghua Jiao, Yuheng Wang, Xiaofeng Sun, Xiaojing Jian. Midazolam induces A549 cell apoptosis International journal of clinical and experimental pathology. vol 11. issue 3. 2020-03-25. PMID:31938232. |
apoptosis of the nsclc cells in response to midazolam was determined by mtt assay, flow cytometry, and western blot. |
2020-03-25 |
2023-08-13 |
Not clear |
| Xingbin Dai, Bangyun Ma, Pengjun Jiang, Zuqiong Xu, Xiangtu Kong, Xuemei Su. Tenacigenin B Has Anti-Tumor Effect in Lymphoma by In Vitro and In Vivo Study. Medical science monitor : international medical journal of experimental and clinical research. vol 25. 2020-01-24. PMID:31473762. |
measuring the cell proliferation of difference groups was done by mtt assay; cell apoptosis and cell cycle of difference groups were evaluated by flow cytometer; relative mrna expression was evaluated by real-time polymerase chain reaction (rt-pcr), and relative protein expressions were measured by western blot assay in an in vitro study. |
2020-01-24 |
2023-08-13 |
mouse |
| Yiqing Liu, Li Xu, Bingru Lu, Miaoqing Zhao, Li Li, Wenping Sun, Zhanjun Qiu, Bingchang Zhan. LncRNA H19/microRNA-675/PPARα axis regulates liver cell injury and energy metabolism remodelling induced by hepatitis B X protein via Akt/mTOR signalling. Molecular immunology. vol 116. 2020-01-22. PMID:31574452. |
mrna and mir-675 levels were determined by quantitative real-time pcr (qrt-pcr), protein levels were determined by western blot, cell viability was measured by the mtt assay, cell apoptosis was measured by flow cytometry, inflammatory cytokine production was determined by elisa, oxidative stress and energy metabolism were assessed by commercial kits, and the target relationship between pparα and mir-675 was confirmed by the dual-luciferase reporter assay. |
2020-01-22 |
2023-08-13 |
Not clear |
| Dan Zhou, Wei Zhai, Miaomiao Zhan. Mesenchymal stem cell-derived extracellular vesicles promote apoptosis in RSC96 Schwann cells through the activation of the ERK pathway. International journal of clinical and experimental pathology. vol 11. issue 11. 2020-01-21. PMID:31949596. |
the effects of msc-evs on rsc96 cell proliferation, migration, apoptosis, and the protein levels were analyzed using the mtt and colony-forming assays, the transwell and wound healing assays, flow cytometry, and western blot, respectively. |
2020-01-21 |
2023-08-13 |
rat |
| Wanida Sukketsiri, Supita Tanasawet, Furoida Moolsap, Mayuree H Tantisira, Pilaiwanwadee Hutamekalin, Varomyalin Tipmane. ECa 233 Suppresses LPS-Induced Proinflammatory Responses in Macrophages via Suppressing ERK1/2, p38 MAPK and Akt Pathways. Biological & pharmaceutical bulletin. vol 42. issue 8. 2020-01-14. PMID:31366870. |
this study aimed to investigate its anti-inflammatory effect and mechanisms of eca 233 in lipopolysaccharide (lps)-stimulated raw264.7 macrophages, through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, nitric oxide (no) assay, reactive oxygen species (ros) production assay, enzyme-linked immunosorbent assay (elisa) and western blot analysis. |
2020-01-14 |
2023-08-13 |
Not clear |
| Yih-Gang Goan, Wen-Tung Wu, Chih-I Liu, Choo-Aun Neoh, Yu-Jen W. Involvement of Mitochondrial Dysfunction, Endoplasmic Reticulum Stress, and the PI3K/AKT/mTOR Pathway in Nobiletin-Induced Apoptosis of Human Bladder Cancer Cells. Molecules (Basel, Switzerland). vol 24. issue 16. 2020-01-09. PMID:31398899. |
using an mtt assay, agarose gel electrophoresis, a wound-healing assay, flow cytometry, and western blot analysis, this study investigated the signaling pathways involved in nob-induced apoptosis in bftc human bladder cancer cells. |
2020-01-09 |
2023-08-13 |
human |
| Hala Soltana, Aline Pinon, Youness Limami, Younes Zaid, Loubna Khalki, Nabil Zaid, Driss Salah, Uteuliyev Yerzhan Sabitaliyevich, Alain Simon, Bertrand Liagre, Mohamed Hammam. Antitumoral activity of Ficus carica L. on colorectal cancer cell lines. Cellular and molecular biology (Noisy-le-Grand, France). vol 65. issue 6. 2020-01-08. PMID:31472041. |
we evaluated the effects of increasing concentrations of ficus carica extracts and latex on hct-116 and ht-29 human colorectal cell proliferation using mtt assay and apoptosis induction by evaluating parp cleavage by western blot analysis. |
2020-01-08 |
2023-08-13 |
human |
| You Cheng, Lunhui Huang, Yichao Wang, Qianyu Huo, Yanhong Shao, Huijing Bao, Zhaoyang Li, Yunde Liu, Xue L. Strontium promotes osteogenic differentiation by activating autophagy via the the AMPK/mTOR signaling pathway in MC3T3‑E1 cells. International journal of molecular medicine. vol 44. issue 2. 2019-12-23. PMID:31173178. |
the effects of sr on cell proliferation and osteogenic differentiation were analyzed by mtt assay, rt‑qpcr, western blot analysis, alkaline phosphatase (alp) and alizarin red staining assays. |
2019-12-23 |
2023-08-13 |
Not clear |
| Jianqiang Zhang, Jing Wang, Xinan Wu, Yuhui We. Ginsenoside Rb1 inhibits proliferation and promotes apoptosis by regulating HMGB1 in uterine fibroid cells. Artificial cells, nanomedicine, and biotechnology. vol 47. issue 1. 2019-12-16. PMID:31313594. |
to study the effects of ginsenoside rb1 and the molecular mechanisms on proliferation and apoptosis of uterine fibroid cells, rb1 + pc dna3.1, rb1 + pc dna3.1-hmgb1, si-nc or si-hmgb1 was transfected into uterine fibroid cells by liposome method; the inhibitory rate and proliferation of human uterine fibroid cells were detected by mtt assay; apoptosis of uterine fibroid cells was detected by flow cytometry assay; hmgb1 protein expression in uterine fibroid cells was detected by western blot assay. |
2019-12-16 |
2023-08-13 |
human |