| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Ke Li, Conghu Yua. MicroRNA‑103 modulates tumor progression by targeting KLF7 in non‑small cell lung cancer. International journal of molecular medicine. vol 46. issue 3. 2021-05-07. PMID:32582959. |
in addition, a series of methods, including mtt, colony formation, 5‑ethynyl‑2'‑deoxyuridine, transwell, wound healing, flow cytometric, reverse transcription‑quantitative pcr and western blot assays, were performed, which revealed that overexpression of mir‑103 enhanced cell growth, migration, invasion and epithelial‑mesenchymal transition (emt), and suppressed apoptosis of a549 and h1299 cells. |
2021-05-07 |
2023-08-13 |
Not clear |
| Bendan Long, Yun Wan, Siqin Zhang, Lisa L. LncRNA XIST protects podocyte from high glucose-induced cell injury in diabetic nephropathy by sponging miR-30 and regulating AVEN expression. Archives of physiology and biochemistry. 2021-05-06. PMID:33332155. |
further wb (western blot), mtt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), and flow cytometry confirmed that xist could reverse the expression of aven and ameliorate hg-induced apoptosis. |
2021-05-06 |
2023-08-13 |
Not clear |
| Fengyu Wang, Fengli Wang, Shengyu Zhang, Xiaogang X. MicroRNA-325 inhibits the proliferation and induces the apoptosis of T cell acute lymphoblastic leukemia cells in a BAG2-dependent manner. Experimental and therapeutic medicine. vol 21. issue 6. 2021-05-04. PMID:33936287. |
the effects of mir-325 mimic and bag2 downregulation on the proliferation and apoptosis were assessed by an mtt assay, flow cytometry and western blot analysis. |
2021-05-04 |
2023-08-13 |
Not clear |
| Jiong Zhang, Xiaojun Den. Effects of miR-599 targeting YAP1 on proliferation, invasion and apoptosis of bladder urothelial carcinoma cells. Experimental and molecular pathology. vol 118. 2021-04-27. PMID:33359177. |
j82 cells cultured in vitro were divided into mir-nc group (transfected with analog negative control mir-nc), mir-599 group (transfected with mir-599 analog), mir-599 + pcdna3.1 group (co transfected with mir-599 analog and pcdna3.1 empty vector), mir-599 + pcdna3.1-yap1 group (co transfected with mir-599 analog and pcdna3.1-yap1 over expression vector), the targeting relationship between mir-599 and yap1 was detected by double luciferase reporter gene assay, the expression level of mir-599 was detected by real-time fluorescence quantitative pcr, the expression of yap1 was detected by western blot, cell proliferation was detected by mtt method, cell invasion was detected by transwell's cell experiment, and apoptosis was detected by flow cytometry. |
2021-04-27 |
2023-08-13 |
Not clear |
| Chunfang Han, Haiying Yu, Qingxia Zho. Calcitriol Attenuates HRV-Induced Respiratory Injury through the AMPK-mTOR-ER Stress Signaling Pathway. Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer. vol 40. issue 2. 2021-04-08. PMID:33822515. |
after hrv infection and treatment with calcitriol, the changes of cell viability were detected by mtt assay, the expression of er stress-induced apoptosis and ampk-mtor related proteins by western blot, and the cell apoptosis by flow cytometry assay. |
2021-04-08 |
2023-08-13 |
human |
| Yingchun Zhang, Xiaojuan Chu, Qingjie We. MiR-451 Promotes Cell Apoptosis and Inhibits Autophagy in Pediatric Acute Myeloid Leukemia by Targeting HMGB1. Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer. vol 40. issue 2. 2021-04-08. PMID:33822516. |
after cells were co-transfected with pcdna-hmgb1 and pc-dna-ctrl, we investigated apoptosis and autophagy in mir-451 overexpressed cells perturbed by exogenous hmgb1 through mtt, flow cytometry, and western blot. |
2021-04-08 |
2023-08-13 |
Not clear |
| Baiqi Wang, Lili Xu, Ju Zhang, Xinru Cheng, Qianya Xu, Jian Wang, Fengxia Ma. LncRNA NORAD accelerates the progression and doxorubicin resistance of neuroblastoma through up-regulating HDAC8 via sponging miR-144-3p. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 129. 2021-02-26. PMID:32563146. |
the proliferation, chemoresistance, apoptosis, metastasis and autophagy of nb cells were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt), flow cytometry, transwell migration and invasion assays and western blot assay, respectively. |
2021-02-26 |
2023-08-13 |
Not clear |
| Yu Liao, Ziyan Wang, Lixin Wang, Yanzhao Lin, Ziyi Ye, Xufang Zeng, Fangning We. MicroRNA-27a-3p directly targets FosB to regulate cell proliferation, apoptosis, and inflammation responses in immunoglobulin a nephropathy. Biochemical and biophysical research communications. vol 529. issue 4. 2021-02-11. PMID:32819575. |
methylthiazolyldiphenyl-tetrazolium bromide (mtt), flow cytometry, real-time polymerase chain reaction, western blot, and enzyme-linked immunosorbent assays were used to verify the regulatory effects of mir-27a-3p and its inhibition on cell proliferation, apoptosis, and release of inflammatory factors in podocytes and hk2 cells. |
2021-02-11 |
2023-08-13 |
human |
| Shaoting Zhang, Guangrong Zhu, Jun Shi, Jihui Yang, Zongying Jiang, Liangying Zhang, Kaikai Dou, Jianmin Su. [PI3K isoforms PI3Kβ and PI3Kδ play different roles in KIT mutation-mediated cell transformation]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 37. issue 1. 2021-02-01. PMID:33441227. |
gist-t1 cells were treated with the same drug, and the activation of kit and its downstream signals was also detected by immunoprecipitation and western blot analysis, and cell proliferation and apoptosis were detected by mtt assay and flow cytometry, respectively. |
2021-02-01 |
2023-08-13 |
Not clear |
| Xueyan Zhang, Wen Liu, Shuxiang We. Luteolin affects keloid fibroblast proliferation and apoptosis by regulating FRAT1 gene expression. Cellular and molecular biology (Noisy-le-Grand, France). vol 66. issue 3. 2021-01-22. PMID:32538769. |
mtt method, western blot, flow cytometry, and real-time quantitative pcr (qpcr) were used to detect cell proliferation, cyclin d1 (cyclind1), p21, b-cell lymphoma / leukemia-2 (bcl-2), bcl-2 related x protein (bax), frat1 protein expression, apoptosis and arhi mrna expression. |
2021-01-22 |
2023-08-13 |
Not clear |
| Shenyu Wang, Gongyin Zhao, Shujie Zhao, Yusen Qiao, Huilin Yan. The Effects of Interleukin-33 (IL-33) on Osteosarcoma Cell Viability, Apoptosis, and Epithelial-Mesenchymal Transition are Mediated Through the PI3K/AKT Pathway. Medical science monitor : international medical journal of experimental and clinical research. vol 26. 2021-01-05. PMID:32312946. |
the effects of il-33 on human osteosarcoma cell viability, apoptosis, emt, and the signaling pathways were studied using the mtt assay, flow cytometry, qrt-pcr, and western blot. |
2021-01-05 |
2023-08-13 |
human |
| Xiangyu Cao, Mingyang Fu, Ruochen Bi, Xiaomei Zheng, Baorong Fu, Siqi Tian, Chengying Liu, Qijiu Li, Jianli Li. Cadmium induced BEAS-2B cells apoptosis and mitochondria damage via MAPK signaling pathway. Chemosphere. vol 263. 2020-12-21. PMID:33297271. |
cell viability, mitochondrial membrane potential, reactive oxygen species (ros) level, apoptosis and the related signaling pathways were detected with mtt assay, rhodamine staining, dcfh-da staining, hoechst33258 staining and western blot methods respectively. |
2020-12-21 |
2023-08-13 |
human |
| Jing Zhao, Kai Zhou, Liang Ma, Huanyu Zhan. MicroRNA-145 overexpression inhibits neuroblastoma tumorigenesis Bioengineered. vol 11. issue 1. 2020-12-14. PMID:32083506. |
the effect of mir-145 on cell viability was evaluated using mtt assays, apoptosis levels were determined using tunel staining, and the mtdh protein expression was determined using western blot and rt-pcr. |
2020-12-14 |
2023-08-13 |
Not clear |
| Paksiree Saranaruk, Ryusho Kariya, Gunya Sittithumcharee, Parichart Boueroy, Thidarut Boonmars, Kanlayanee Sawanyawisuth, Chaisiri Wongkham, Sopit Wongkham, Seiji Okada, Kulthida Vaeteewoottachar. Chromomycin A3 suppresses cholangiocarcinoma growth by induction of S phase cell cycle arrest and suppression of Sp1‑related anti‑apoptotic proteins. International journal of molecular medicine. vol 45. issue 4. 2020-11-30. PMID:32124934. |
the effects of cma3 on anti‑proliferation, cell cycle arrest and apoptosis induction in cca cells were demonstrated by mtt assay, flow cytometry and western blot analysis. |
2020-11-30 |
2023-08-13 |
mouse |
| Claudia G Fresta, Annamaria Fidilio, Giacomo Lazzarino, Nicolò Musso, Margherita Grasso, Sara Merlo, Angela M Amorini, Claudio Bucolo, Barbara Tavazzi, Giuseppe Lazzarino, Susan M Lunte, Filippo Caraci, Giuseppe Carus. Modulation of Pro-Oxidant and Pro-Inflammatory Activities of M1 Macrophages by the Natural Dipeptide Carnosine. International journal of molecular sciences. vol 21. issue 3. 2020-11-05. PMID:31991717. |
an ample set of parameters aimed to evaluate cytotoxicity (mtt assay), energy metabolism (hplc), gene expressions (high-throughput real-time pcr (qrt-pcr)), protein expressions (western blot) and nitric oxide production (qrt-pcr and hplc), was used to assess the effects of carnosine on activated macrophages challenged with a non cytotoxic lps (100 ng/ml) + ifn-γ (600 u/ml) concentration. |
2020-11-05 |
2023-08-13 |
cat |
| Beyza Goncu, Ece Sevgi, Cagla Kizilarslan Hancer, Guzin Gokay, Nur Ozte. Differential anti-proliferative and apoptotic effects of lichen species on human prostate carcinoma cells. PloS one. vol 15. issue 9. 2020-10-26. PMID:32997661. |
lichen extracts were performed by ethanol, methanol, and acetone separately by using the soxhlet apparatus and the effects of the extracts on cell viability, proliferation, and apoptosis were measured with the utilization of mtt, ldh assay, annexin v assay, and western blot. |
2020-10-26 |
2023-08-13 |
human |
| Yueran Li, Songshu Xiao, Liu Dan, Min Xu. P16 Oncology letters. vol 9. issue 3. 2020-10-01. PMID:25663864. |
in addition, the proliferation and apoptosis rates of p16 knockdown siha-ddp cells were measured by mtt assay and annexin v flow cytometry and the subsequent changes in cyclin d1 and prb expression were analyzed by western blot analysis. |
2020-10-01 |
2023-08-13 |
human |
| Yunhua Tang, Xiangyang Zhang, Fan Qi, Mingfeng Chen, Yuan Li, Longfei Liu, Wei He, Zhuo Li, Xiongbing Z. Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line. Experimental and therapeutic medicine. vol 9. issue 5. 2020-10-01. PMID:26136904. |
mtt assay was used to estimate the proliferation of the t24 cells; flow cytometric analysis was used to estimate the effect of afatinib on t24 cell apoptosis; cell invasion ability was assessed by a transwell invasion assay; and western blot analysis was used to detect the expression of bcl-2, bax, akt, extracellular-signal-regulated kinase (erk)1/2, matrix metalloproteinase (mmp)-2 and mmp-9. |
2020-10-01 |
2023-08-13 |
human |
| Guangxin Zhang, Mei Sun, Yifan Zhang, Peiyan Hua, Xin Li, Ranji Cui, Xingyi Zhan. Dracorhodin perchlorate induces G Oncology letters. vol 10. issue 1. 2020-10-01. PMID:26171006. |
therefore, the present study aimed to investigate the effects of dp on cell viability, the cell cycle and apoptosis, using an mtt assay, flow cytometry and western blot studies. |
2020-10-01 |
2023-08-13 |
human |
| Jiaxin Li, Xuping Mao, Xing Wang, Ganggang Miao, Jiaxin L. miR-433 reduces cell viability and promotes cell apoptosis by regulating MACC1 in colorectal cancer. Oncology letters. vol 13. issue 1. 2020-10-01. PMID:28123526. |
furthermore, flow cytometry and mtt assays were used to examine the apoptosis, cell cycle distribution and viability of human colorectal cancer cells, and luciferase reporter and western blot assays were performed to verify the regulatory mechanism of mir-433 on macc1. |
2020-10-01 |
2023-08-13 |
human |