| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Zhi-Jian Meng, Ke Ta. Enhancement of Chemosensitivity by Stathmin-1 Silencing in Gastric Cancer Cells In Situ and In Vivo. Oncology research. vol 23. issue 1. 2019-11-20. PMID:29021019. |
the cell growth inhibition, expression of associated proteins, cell cycle, and apoptosis were evaluated by mtt, western blot, and flow cytometric assays, respectively. |
2019-11-20 |
2023-08-13 |
Not clear |
| Zhibin Wang, Xuan Zhao, Zhiming Ma, Li Liu, Bin Wang, Yuan L. Modulation on gallbladder carcinoma by TGF-β1 via IGFBP-2. Cancer biomarkers : section A of Disease markers. 2019-11-20. PMID:30614799. |
the aim of this study was to investigate the effect and mechanism of tgf-β in gc using gc cell line noz cells.in vitro cultured noz cell was randomly assigned into control, si-nc and tgf-β1 sirna groups and were transfected with sirna negative control (nc) or tgf-β1 sirna followed by analysis of tgf-β1 expression by real-time pcr, cell proliferation by mtt assay, cell apoptosis and cell invasion, as well as expression of proteins in epithelial-mesenchymal transition (emt), p38, smad2/3 and smad4 phosphorylation by western blot, insulin-like growth factor-binding protein-2 (igfbp-2) level by elisa. |
2019-11-20 |
2023-08-13 |
Not clear |
| J Li, S H Zhang, D He, J F Wang, J Q L. Paeoniflorin reduced the cardiotoxicity of aconitine in h9c2 cells. Journal of biological regulators and homeostatic agents. vol 33. issue 5. 2019-11-11. PMID:31576730. |
mtt assay, western blot analysis and real-time pcr were used to measure cell proliferation, apoptosis, ion channels and oxidative stress. |
2019-11-11 |
2023-08-13 |
Not clear |
| Tao Yu, Ji Qu, Yang Wang, Hui Ji. Ligustrazine protects chondrocyte against IL-1β induced injury by regulation of SOX9/NF-κB signaling pathway. Journal of cellular biochemistry. vol 119. issue 9. 2019-10-17. PMID:29806884. |
cell viability, apoptosis, and apoptosis-related factors were examined by mtt, flow cytometry, and western blot. |
2019-10-17 |
2023-08-13 |
human |
| S Li, J J L. Zoledronic acid modulates human osteosarcoma cells proliferation via GSK-3β activation. Neoplasma. vol 66. issue 5. 2019-10-07. PMID:31288526. |
the effects of gsk-3β on zol-induced apoptosis among these groups were characterized by flow cytometry, mtt assay, transmission electron microscopy (tem) and western blot. |
2019-10-07 |
2023-08-13 |
human |
| Ying Xie, Xu Yan, Ling Su. The Mechanism of Bufalin-Induced Apoptosis of K562/A02. Medical science monitor : international medical journal of experimental and clinical research. vol 25. 2019-10-02. PMID:30955024. |
we used mtt and flow cytometry to detect apoptosis, and rt-pcr and western blot were used to detect endoplasmic reticulum stress and apoptosis gene action. |
2019-10-02 |
2023-08-13 |
Not clear |
| Jing-Kang Wang, Li-Chao Wang, Yong Jiang, Peng-Fei Tu, Ke-Wu Zen. [Neuroprotective effects and mechanism of ethanol extract of Cistanche tubulosa against oxygen-glucose deprivation/reperfusion]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 44. issue 13. 2019-09-24. PMID:31359678. |
5,25,50 mg·l-1) by detecting cell viability with mtt assay,apoptosis with ao/eb and hoechst 33258,mitochondrial membrane potential changes with jc-1 staining,mitochondrial oxidative stress with mitosox staining,as well as the apoptosis-related protein expression( parp,cleaved parp,caspase-3,cleaved caspase-3,bax,bcl-2) with western blot. |
2019-09-24 |
2023-08-13 |
Not clear |
| Ulises Daniel Orlando, Ana Fernanda Castillo, Mayra Agustina Ríos Medrano, Angela Rosaria Solano, Paula Mariana Maloberti, Ernesto Jorge Podest. Acyl-CoA synthetase-4 is implicated in drug resistance in breast cancer cell lines involving the regulation of energy-dependent transporter expression. Biochemical pharmacology. vol 159. 2019-09-03. PMID:30414939. |
assays were conducted on cell viability (mtt), cell proliferation (brdu), drug efflux (flow cytometry), acsl4-responsive drug resistance abc transporter genes (rna-seq), transporter mrna expression, protein levels and signaling pathway participation (real-time pcr and western blot). |
2019-09-03 |
2023-08-13 |
Not clear |
| Chunyang Kang, Mingyang Kang, Yingying Han, Tuo Zhang, Wei Quan, Jian Ga. 6-Gingerols (6G) reduces hypoxia-induced PC-12 cells apoptosis and autophagy through regulation of miR-103/BNIP3. Artificial cells, nanomedicine, and biotechnology. vol 47. issue 1. 2019-09-02. PMID:31043087. |
cell viability, apoptosis and its related proteins were studied by the approaches of mtt assay, flow cytometry assay and western blot analysis, respectively. |
2019-09-02 |
2023-08-13 |
rat |
| Yanhua Wang, Bo Li, Xiaofen Zhan. Scutellaria barbata D. Don (SBD) protects oxygen glucose deprivation/reperfusion-induced injuries of PC12 cells by up-regulating Nrf2. Artificial cells, nanomedicine, and biotechnology. vol 47. issue 1. 2019-08-29. PMID:31062620. |
cell viability, apoptosis and proliferation were detected using mtt assay, flow cytometry, ki67 staining and western blot. |
2019-08-29 |
2023-08-13 |
Not clear |
| Ning Zhang, Ketao Wang, Zhongli Li, Tao Lu. Comparative study of different anticoagulants and coagulants in the evaluation of clinical application of platelet-rich plasma (PRP) standardization. Cell and tissue banking. vol 20. issue 1. 2019-08-02. PMID:30729369. |
the effects of different coagulants with prp on proliferation, osteogenic differentiation of bmscs were analyzed by methyl thiazolyl tetrazolium assay (mtt), alp staining, von kossa staining, confocal microscopic observation, rt-pcr and western blot at the morphological, cellular and molecular levels. |
2019-08-02 |
2023-08-13 |
Not clear |
| Dingyun Li, Tao Zhang, Jiajun Lai, Jian Zhang, Ting Wang, Yafei Ling, Shengquan He, Zhiwei H. MicroRNA‑25/ATXN3 interaction regulates human colon cancer cell growth and migration. Molecular medicine reports. vol 19. issue 5. 2019-08-01. PMID:30942397. |
cell viability and cell migration were analyzed using mtt and wound healing assays, respectively, apoptosis was analyzed using flow cytometry, and western blot analysis was conducted to determine the protein expression of ataxin‑3 (atxn3), apoptosis regulator bax (bax) and cyclin d1. |
2019-08-01 |
2023-08-13 |
human |
| Jie Zheng, Su Min, Bin Hu, Qin Liu, Yunqiang Wa. Nrdp1 is involved in hippocampus apoptosis in cardiopulmonary bypass-induced cognitive dysfunction via the regulation of ErbB3 protein levels. International journal of molecular medicine. vol 43. issue 4. 2019-07-30. PMID:30720051. |
apoptosis in the hippocampus regions were measured using a terminal deoxynucleotidyl‑transferase‑mediated dutp nick end labelling assay, the viability and apoptosis level of the cells were measured via an mtt assay and flow cytometry, respectively, and the expression levels of nrdp1, erb‑b2 receptor tyrosine kinase 3 (erbb3), phosphorylated‑protein kinase b (p‑akt) and cleaved (c‑) caspase‑3 were detected using western blot analysis. |
2019-07-30 |
2023-08-13 |
rat |
| Chia-Cheng Su, Kun-Lin Hsieh, Po-Len Liu, Hsin-Chih Yeh, Shu-Pin Huang, Shih-Hua Fang, Wei-Chung Cheng, Kuan-Hua Huang, Fang-Yen Chiu, I-Ling Lin, Ming-Yii Huang, Chia-Yang L. AICAR Induces Apoptosis and Inhibits Migration and Invasion in Prostate Cancer Cells Through an AMPK/mTOR-Dependent Pathway. International journal of molecular sciences. vol 20. issue 7. 2019-07-30. PMID:30987073. |
cell growth was performed by mtt assay and soft agar assay; cell apoptosis was examined by annexin v/propidium iodide (pi) staining and poly adp ribose polymerase (parp) cleavage western blot, while cell migration and invasion were evaluated by wound-healing assay and transwell assay respectively. |
2019-07-30 |
2023-08-13 |
Not clear |
| Biao Cai, Shu Ye, Yan Wang, Ting-Ting Wang, Liang Wang, Ai-Juan Jiang, Zheng-Qing Fang, Guo-Ming Shen, Dao-Jun Xi. [Effects of Huangpu Tongqiao capsule on apoptosis of Alzheimer's disease cell model]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 43. issue 11. 2019-07-18. PMID:29945394. |
after primary cultured hippocampal neurons ad cell model was induced by aβ₂₅₋₃₅, cell survival rate was detected by mtt, cell apoptosis rate was assayed by flow cytometry, and protein expressions of bax, cyt c and caspase-3 were determined by western blot analysis. |
2019-07-18 |
2023-08-13 |
Not clear |
| Chang Min Lee, Jongsung Lee, Myeong Jin Nam, Youn Soo Choi, See-Hyoung Par. Tomentosin Displays Anti-Carcinogenic Effect in Human Osteosarcoma MG-63 Cells via the Induction of Intracellular Reactive Oxygen Species. International journal of molecular sciences. vol 20. issue 6. 2019-07-09. PMID:30917517. |
after treatment with tomentosin, mg-63 cells were analyzed using the mtt assay, colony formation assay, cell counting assay, wound healing assay, boyden chamber assay, zymography assay, cell cycle analysis, fitc annexin v apoptosis assay, terminal deoxynucleotidyl transferase dutp nick end labeling assay, western blot analysis, and ros detection analysis. |
2019-07-09 |
2023-08-13 |
human |
| Jiping Gao, Yu Wang, Guoqiang Xu, Jianing Wei, Kai Chang, Xiaolin Tian, Maolin Liu, Xiaoyan Yan, Meijun Huo, Guohua Son. Selenium attenuates apoptosis and p-AMPK expressions in fluoride-induced NRK-52E cells. Environmental science and pollution research international. vol 26. issue 15. 2019-07-02. PMID:30949948. |
at present, in this study, the normal rat kidney cell (nrk-52e) was used to investigate the potentially protective mechanism of se against naf-induced apoptosis, by using the methods of pathology, colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, flow cytometry, and western blot. |
2019-07-02 |
2023-08-13 |
rat |
| Da Gao, Zhen Xiao, Hui-Ping Li, Dong-Hai Han, Ya-Peng Zhan. The mechanism study of miR-125b in occurrence and progression of multiple myeloma. Cancer medicine. vol 7. issue 1. 2019-06-24. PMID:29214735. |
the influence of lenti-sh-mir-125b on cell viability and the notch-1 pathway-related proteins were assessed by mtt method and western blot, respectively. |
2019-06-24 |
2023-08-13 |
mouse |
| Xiaogang Tang, Qiulong Ding, Chao Chen, Fei Chen, Xiyang Zhou, Chu Jie Hong, Wensheng Pa. Micheliolide inhibits gastric cancer growth Die Pharmazie. vol 74. issue 3. 2019-06-21. PMID:30961685. |
in this study, we detected the effects of mcl on gastric cancer cell proliferation and apoptosis by performing mtt, colony formation, tunel and western blot assays, and found that mcl inhibited gastric cancer cell proliferation and promoted apoptosis |
2019-06-21 |
2023-08-13 |
Not clear |
| Song Yang, Wanzhong Tang, Yuchao He, Linbao Wen, Bin Sun, Shengli L. Long non-coding RNA and microRNA-675/let-7a mediates the protective effect of melatonin against early brain injury after subarachnoid hemorrhage via targeting TP53 and neural growth factor. Cell death & disease. vol 9. issue 2. 2019-06-12. PMID:29367587. |
real-time pcr, western blot analysis, immunohistochemistry, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, and flow cytometry analysis were performed to detect the effect of mt on h19, mir-675, let-7a, tp53, neural growth factor (ngf) levels, cell viability, and apoptosis status. |
2019-06-12 |
2023-08-13 |
Not clear |