| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| F Zhu, W Yan, Z L Zhao, Y B Chai, F Lu, Q Wang, W D Peng, A G Yang, C J Wan. Improved PCR-based subtractive hybridization strategy for cloning differentially expressed genes. BioTechniques. vol 29. issue 2. 2001-02-01. PMID:10948432. |
twenty-seven clones related to apoptosis were identified by reverse dot blot assay. |
2001-02-01 |
2023-08-12 |
human |
| F Zhu, W Yan, Z L Zhao, Y B Chai, F Lu, Q Wang, W D Peng, A G Yang, C J Wan. Improved PCR-based subtractive hybridization strategy for cloning differentially expressed genes. BioTechniques. vol 29. issue 2. 2001-02-01. PMID:10948432. |
apr-1, apr-2, apr-3 and tnf were reidentified by reverse dot blot, and it is suggested that they might be related to apoptosis. |
2001-02-01 |
2023-08-12 |
human |
| K C Wang, A L Cheng, S E Chuang, H C Hsu, I J S. Retinoic acid-induced apoptotic pathway in T-cell lymphoma: Identification of four groups of genes with differential biological functions. Experimental hematology. vol 28. issue 12. 2001-01-25. PMID:11146166. |
the cloned gene fragments were then analyzed and confirmed by northern blot analysis on h9 and sr786 cell lines.atra-induced apoptosis of t-cell lymphoma was protein synthesis-dependent. |
2001-01-25 |
2023-08-12 |
Not clear |
| Q N Diep, R M Touyz, E L Schiffri. Docosahexaenoic acid, a peroxisome proliferator-activated receptor-alpha ligand, induces apoptosis in vascular smooth muscle cells by stimulation of p38 mitogen-activated protein kinase. Hypertension (Dallas, Tex. : 1979). vol 36. issue 5. 2000-12-28. PMID:11082155. |
to clarify possible pathways of apoptosis, we evaluated the expression of phosphorylated p38 mitogen-activated protein kinases, bax, bcl-2, cytochrome c, and peroxisome proliferator-activated receptor-alpha (ppar-alpha) with western blot analysis. |
2000-12-28 |
2023-08-12 |
rat |
| R Moriya, T Uehara, Y Nomur. Mechanism of nitric oxide-induced apoptosis in human neuroblastoma SH-SY5Y cells. FEBS letters. vol 484. issue 3. 2000-12-22. PMID:11078888. |
western blot analysis showed that pro-forms of caspase-2, -3, -6, and -7 are decreased during apoptosis. |
2000-12-22 |
2023-08-12 |
human |
| J J Lysiak, S D Turner, T T Turne. Molecular pathway of germ cell apoptosis following ischemia/reperfusion of the rat testis. Biology of reproduction. vol 63. issue 5. 2000-12-15. PMID:11058553. |
proteins from seminiferous tubules were used for western blot analysis of cytochrome c. germ cell apoptosis was maximal at 24 h after repair of torsion. |
2000-12-15 |
2023-08-12 |
rat |
| J C Shen, R D Klein, Q Wei, Y Guan, J H Contois, T T Wang, S Chang, S D Hurstin. Low-dose genistein induces cyclin-dependent kinase inhibitors and G(1) cell-cycle arrest in human prostate cancer cells. Molecular carcinogenesis. vol 29. issue 2. 2000-11-30. PMID:11074606. |
viable cell number was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay; cell-cycle progression and apoptosis were evaluated by flow cytometry; apoptosis was also assessed by a histone enzyme-linked immunosorbent assay; and the expression of several cell-cycle- and apoptosis-related genes and their gene products was determined by northern blot analysis, western blot analysis, and/or assays based on polymerase chain reaction. |
2000-11-30 |
2023-08-12 |
human |
| H Sasaki, Y Sheng, F Kotsuji, B K Tsan. Down-regulation of X-linked inhibitor of apoptosis protein induces apoptosis in chemoresistant human ovarian cancer cells. Cancer research. vol 60. issue 20. 2000-11-08. PMID:11059757. |
apoptosis and protein expression (e.g., xiap, caspase-3, p53, mdm2, and p21waf1) were assessed by hoechst 33258 stain and western blot, respectively. |
2000-11-08 |
2023-08-12 |
human |
| T E Vaskivuo, F Stenbäck, P Karhumaa, J Risteli, L Dunkel, J S Tapanaine. Apoptosis and apoptosis-related proteins in human endometrium. Molecular and cellular endocrinology. vol 165. issue 1-2. 2000-11-07. PMID:10940486. |
apoptosis was studied quantitatively by southern blot analysis of internucleosomal cleavage of genomic dna, and qualitatively by using in situ 3'-end labeling of fragmented dna, and bcl-2, bax and ki-67 were detected and quantified immunohistochemically. |
2000-11-07 |
2023-08-12 |
human |
| L S Wang, H J Liu, Z B Xia, H E Broxmeyer, L L. Expression and activation of caspase-3/CPP32 in CD34(+) cord blood cells is linked to apoptosis after growth factor withdrawal. Experimental hematology. vol 28. issue 8. 2000-10-10. PMID:10989191. |
reverse transcriptase-polymerase chain reaction (rt-pcr), western blot, and flow cytometry analysis were used in this study to determine the expression of caspase-3/cpp32 in cd34(+) cb cells during apoptosis. |
2000-10-10 |
2023-08-12 |
Not clear |
| N Mitsiades, V Poulaki, S Tseleni-Balafouta, D A Koutras, I Stamenkovi. Thyroid carcinoma cells are resistant to FAS-mediated apoptosis but sensitive to tumor necrosis factor-related apoptosis-inducing ligand. Cancer research. vol 60. issue 15. 2000-08-31. PMID:10945619. |
we addressed the expression of fas, dr4, and dr5 in thyroid carcinoma cell lines and in 31 thyroid carcinoma specimens by western blot analysis and immunohistochemistry, respectively, and tested the sensitivity of thyroid carcinoma cell lines to fas- and trail-induced apoptosis. |
2000-08-31 |
2023-08-12 |
Not clear |
| D Dai, B C Moulton, T F Ogl. Regression of the decidualized mesometrium and decidual cell apoptosis are associated with a shift in expression of Bcl2 family members. Biology of reproduction. vol 63. issue 1. 2000-08-24. PMID:10859259. |
expression of bax and bcl2 and the incidence of apoptosis were evaluated throughout gestation by western blot analysis and detection of dna fragments. |
2000-08-24 |
2023-08-12 |
rat |
| J Xu, Z Xu, Y Jiang, X Qian, Y Huan. Cryptorchidism induces mouse testicular germ cell apoptosis and changes in bcl-2 and bax protein expression. Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer. vol 19. issue 1-2. 2000-08-22. PMID:10905505. |
western blot analysis showed that the bcl-2 protein down-regulated and the bax protein up-regulated apoptosis. |
2000-08-22 |
2023-08-12 |
mouse |
| W J Zhang, K Ohnishi, H Yoshida, L Pan, L Maksumova, F Muratkhodjaev, J M Luo, K Shigeno, S Fujisawa, K Naito, S Nakamura, K Shinjo, A Takeshita, R Ohn. Spicamycin and KRN5500 induce apoptosis in myeloid and lymphoid cell lines with down-regulation of bcl-2 expression and modulation of promyelocytic leukemia protein. Japanese journal of cancer research : Gann. vol 91. issue 6. 2000-08-18. PMID:10874212. |
western blot analysis of bcl-2, bcl-xl and bax expression and immunofluorescence analysis of promyelocytic leukemia (pml) protein indicated that apoptosis induced by spicamycin and krn5500 was associated with down-regulation of bcl-2 expression and modulation of pml protein. |
2000-08-18 |
2023-08-12 |
mouse |
| A P Simões-Wüst, R A Olie, O Gautschi, S H Leech, R Häner, J Hall, D Fabbro, R A Stahel, U Zangemeister-Wittk. Bcl-xl antisense treatment induces apoptosis in breast carcinoma cells. International journal of cancer. vol 87. issue 4. 2000-08-18. PMID:10918201. |
the antisense effect of oligonucleotide 4259 was examined on the mrna and protein level using real-time pcr and western blot analysis, respectively, and the induction of cell death was investigated in viability and apoptosis assays. |
2000-08-18 |
2023-08-12 |
Not clear |
| T L Beumer, H L Roepers-Gajadien, I S Gademan, T M Lock, H B Kal, D G De Rooi. Apoptosis regulation in the testis: involvement of Bcl-2 family members. Molecular reproduction and development. vol 56. issue 3. 2000-08-09. PMID:10862001. |
using immunohistochemical techniques and western blot analysis, the possible role of bcl-2 family members bax, bcl-2, bcl-x(s), and bcl-x(l) in male germ cell density-related apoptosis and dna damage induced apoptosis was studied. |
2000-08-09 |
2023-08-12 |
mouse |
| V M González, M A Fuertes, M J Pérez-Alvarez, G Cervantes, V Moreno, C Alonso, J M Pére. Induction of apoptosis by the bis-Pt(III) complex [Pt(2)(2-mercaptopyrimidine)(4)Cl(2)]. Biochemical pharmacology. vol 60. issue 3. 2000-07-13. PMID:10856432. |
interestingly, western blot data indicated that apoptosis induction by pt-spym in hela, ch1, and ch1cisr cells was not associated with drastic changes in p53 levels. |
2000-07-13 |
2023-08-12 |
human |
| N Baghdassarian, Y Bertrand, P Ffrench, P Duhaut, P A Bryon, M Ffrenc. Role of BCL-2 and cell cycle regulatory proteins for corticosensitivity assessment in childhood acute lymphoblastic leukaemia. British journal of haematology. vol 109. issue 1. 2000-07-12. PMID:10848789. |
fifty-two children presenting with all were studied at diagnosis and during the first 48 h of treatment for cell proliferation and apoptosis level by measurement of dna content, and for expression of several cell proliferation regulatory proteins by means of western blot. |
2000-07-12 |
2023-08-12 |
Not clear |
| S J Baker, E P Redd. Cloning of murine G1RP, a novel gene related to Drosophila melanogaster g1. Gene. vol 248. issue 1-2. 2000-07-06. PMID:10806348. |
northern blot analysis using rnas derived from 32dcl3 cells that have been grown in the absence of il-3 demonstrates that the g1rp message is upregulated in these cells following the removal of il-3, suggesting that this gene may regulate growth factor withdrawal-induced apoptosis of myeloid precursor cells. |
2000-07-06 |
2023-08-12 |
drosophila_melanogaster |
| A Zaragoza, C Díez-Fernández, A M Alvarez, D Andrés, M Cascale. Effect of N-acetylcysteine and deferoxamine on endogenous antioxidant defense system gene expression in a rat hepatocyte model of cocaine cytotoxicity. Biochimica et biophysica acta. vol 1496. issue 2-3. 2000-06-29. PMID:10771087. |
lysis of the cell membranes was evidenced by lactate dehydrogenase leakage, apoptosis was observed by detecting a hypodiploid peak (<2c) in dna histograms obtained by flow cytometry, peroxide production was quantified with 2', 7'-dichlorodihydrofluorescein diacetate and gene expression of the antioxidant enzymes: mn- and cu,zn-superoxide dismutases, catalase and glutathione peroxidase were measured by northern blot analysis. |
2000-06-29 |
2023-08-12 |
rat |