| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Y Akao, H Yamada, Y Nakagaw. Arsenic-induced apoptosis in malignant cells in vitro. Leukemia & lymphoma. vol 37. issue 1-2. 2000-05-18. PMID:10721769. |
in addition to the involvement of caspase 3 in arsenic trioxide-induced apoptosis of nb4 cells, the activation of caspase 8 was also shown to be involved by western blot analysis or by apoptosis inhibition assay using caspase 8 inhibitor ac-ietd-cho. |
2000-05-18 |
2023-08-12 |
mouse |
| S B Brown, M C Clarke, L Magowan, H Sanderson, J Savil. Constitutive death of platelets leading to scavenger receptor-mediated phagocytosis. A caspase-independent cell clearance program. The Journal of biological chemistry. vol 275. issue 8. 2000-03-30. PMID:10681593. |
however, and in contrast with constitutive death of other inflammatory blood cells by apoptosis, these events were not affected by caspase inhibitors, nor was there evidence of caspase-3 activation either by hydrolysis of analog peptide substrates or western blot analysis, serving to emphasize that neither programmed cell death nor clearance by phagocytes need involve caspases. |
2000-03-30 |
2023-08-12 |
Not clear |
| A M Rodríguez-López, O Flores, C Martínez-Salgado, N Eleno, J M López-Novoa, M Aréval. Increased apoptosis susceptibility in mesangial cells from spontaneously hypertensive rats. Microvascular research. vol 59. issue 1. 2000-02-25. PMID:10625574. |
we assessed apoptosis by propidium iodide staining, by tunel nitrite production (griess reaction), by inducible nitric oxide synthase (inos) and bcl-2 and bax by western blot. |
2000-02-25 |
2023-08-12 |
rat |
| Y L Sun, Y Zhao, X Hong, Z H Zha. Cytochrome c release and caspase activation during menadione-induced apoptosis in plants. FEBS letters. vol 462. issue 3. 2000-02-02. PMID:10622718. |
western blot analysis indicated that the caspase specific inhibitors ac-devd-cho (ac-asp-glu-val-asp-aldehyde) and ac-yvad-cho (n-acetyl-try-val-ala-aspartinal) inhibited the degradation of a caspase 3 specific substrate parp (poly(adp-ribose) polymerase), and they had no effect on the release of cytochrome c. further study showed that menadione could not induce apoptosis of mouse liver nuclei in tobacco cytosol extract containing no mitochondria. |
2000-02-02 |
2023-08-12 |
mouse |
| L y Xue, J He, N L Oleinic. Promotion of photodynamic therapy-induced apoptosis by stress kinases. Cell death and differentiation. vol 6. issue 9. 2000-01-04. PMID:10510467. |
to ascertain whether activation of one or more mapks could mediate pdt-induced apoptosis, western blot analysis has been performed on the proteins of ly-r and cho cells at various times following lethal (90 - 99% cell kill) doses of pdt photosensitized by the phthalocyanine pc 4. |
2000-01-04 |
2023-08-12 |
Not clear |
| J H Richburg, A Nañez, H Ga. Participation of the Fas-signaling system in the initiation of germ cell apoptosis in young rat testes after exposure to mono-(2-ethylhexyl) phthalate. Toxicology and applied pharmacology. vol 160. issue 3. 1999-12-09. PMID:10544061. |
western blot analysis demonstrated a time-dependent increase in the production of the soluble form of fasl after mehp exposure and suggests that it may participate in triggering apoptosis in germ cells that have lost their intimate association with the sertoli cells. |
1999-12-09 |
2023-08-12 |
rat |
| K Erkkilä, V Pentikäinen, M Wikström, M Parvinen, L Dunke. Partial oxygen pressure and mitochondrial permeability transition affect germ cell apoptosis in the human testis. The Journal of clinical endocrinology and metabolism. vol 84. issue 11. 1999-11-26. PMID:10566681. |
apoptosis was significantly suppressed in an inversely dose-dependent fashion at partial oxygen pressures below 10%, as detected by southern blot analysis of dna fragmentation, dna labeling in situ, and electron microscopy. |
1999-11-26 |
2023-08-12 |
human |
| I Petrache, M E Choi, L E Otterbein, B Y Chin, L L Mantell, S Horowitz, A M Cho. Mitogen-activated protein kinase pathway mediates hyperoxia-induced apoptosis in cultured macrophage cells. The American journal of physiology. vol 277. issue 3. 1999-10-28. PMID:10484467. |
to further delineate the signaling pathway of hyperoxia-induced apoptosis in raw 264.7 macrophages, we first show that hyperoxia can activate the mitogen-activated protein kinase (mapk) pathway, the extracellular signal-regulated kinases (erks) p42/p44, in a time-dependent manner as assessed by increased phosphorylation of erk1/erk2 by western blot analyses. |
1999-10-28 |
2023-08-12 |
mouse |
| L Chen, S Zheng, M C Willingham, W Fa. [Cloning of genes associated with taxol-induced apoptosis of human breast cancer cells]. Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae. vol 19. issue 3. 1999-10-05. PMID:10453491. |
results demonstrated that the expressive alteration of twelve clones isolated was associated with taxol-induced apoptosis identified by northern blot. |
1999-10-05 |
2023-08-12 |
human |
| P C Konturek, T Brzozowski, S J Konturek, R Pajdo, J E Konturek, S Kwiecień, A Taut, E G Hah. Apoptosis in gastric mucosa with stress-induced gastric ulcers. Journal of physiology and pharmacology : an official journal of the Polish Physiological Society. vol 50. issue 2. 1999-09-22. PMID:10424718. |
the apoptosis rate in the gastric mucosa was determined by the terminal deoxynucleotidyl transferase (tdt) mediated 2-deoxyuridine 5-triphosphate (dutp)-biotin nick end-labeling (tunel) staining method and the expression of bax and bcl-2 mrna was analyzed by rt-pcr and southern blot hybridization. |
1999-09-22 |
2023-08-12 |
rat |
| S Nandi, P P Banerjee, B R Zirki. Germ cell apoptosis in the testes of Sprague Dawley rats following testosterone withdrawal by ethane 1,2-dimethanesulfonate administration: relationship to Fas? Biology of reproduction. vol 61. issue 1. 1999-09-03. PMID:10377033. |
apoptosis was examined in situ and biochemically, and fas protein content in the testis was monitored by western blot analysis. |
1999-09-03 |
2023-08-12 |
rat |
| Y Akao, Y Nakagawa, K Akiyam. Arsenic trioxide induces apoptosis in neuroblastoma cell lines through the activation of caspase 3 in vitro. FEBS letters. vol 455. issue 1-2. 1999-08-16. PMID:10428472. |
as2o3-induced apoptosis in neuroblastoma cells was shown to occur through the activation of caspase 3, as judged from western blot analysis and apoptosis inhibition assay. |
1999-08-16 |
2023-08-12 |
Not clear |
| Y Akao, Y Nakagawa, W Maruyama, T Takahashi, M Nao. Apoptosis induced by an endogenous neurotoxin, N-methyl(R)salsolinol, is mediated by activation of caspase 3. Neuroscience letters. vol 267. issue 3. 1999-08-03. PMID:10380999. |
in this study, we tried to elucidate the intracellular mechanism of apoptosis induced by n-methyl(r)salsolinol, and proved activation of caspase 3 after incubation with this toxin by western blot analysis. |
1999-08-03 |
2023-08-12 |
human |
| P C Konturek, P Pierzchalski, S J Konturek, H Meixner, G Faller, T Kirchner, E G Hah. Helicobacter pylori induces apoptosis in gastric mucosa through an upregulation of Bax expression in humans. Scandinavian journal of gastroenterology. vol 34. issue 4. 1999-07-22. PMID:10365897. |
the apoptosis was analyzed by means of terminal deoxyribonucleotide transferase-mediated digoxigenin-11-deoxyuridine triphosphate biotin nick-end labeling (tunel) staining, and the expression of mrna for bax and bcl-2 by reverse transcription polymerase chain reaction (rt-pcr) and southern blot. |
1999-07-22 |
2023-08-12 |
human |
| T T Turner, T A Rile. p53 independent, region-specific epithelial apoptosis is induced in the rat epididymis by deprivation of luminal factors. Molecular reproduction and development. vol 53. issue 2. 1999-07-19. PMID:10331457. |
p53, a key apoptotic-pathway molecule in many tissues and conditions was tested by immunohistochemical and western blot techniques and was not upregulated in the initial segment epithelium within the time cells were undergoing apoptosis and well before the wave of apoptosis was complete. |
1999-07-19 |
2023-08-12 |
rat |
| J Zheng, Y P Deng, C Lin, M Fu, P G Xiao, M W. Arsenic trioxide induces apoptosis of HPV16 DNA-immortalized human cervical epithelial cells and selectively inhibits viral gene expression. International journal of cancer. vol 82. issue 2. 1999-07-19. PMID:10389765. |
this effect was evidenced by a reduction of cell viability in the mtt assay, g1 arrest and significant apoptosis upon flow-cytometric analysis, presence of apoptotic bodies, formation of dna ladders upon gel electrophoresis and inhibition of viral early gene expression by rt-pcr and western blot. |
1999-07-19 |
2023-08-12 |
human |
| R Robles, X J Tao, A M Trbovich, D V Maravel, R Nahum, G I Perez, K I Tilly, J L Till. Localization, regulation and possible consequences of apoptotic protease-activating factor-1 (Apaf-1) expression in granulosa cells of the mouse ovary. Endocrinology. vol 140. issue 6. 1999-06-03. PMID:10342853. |
western blot analysis of fractionated protein extracts prepared from granulosa cells before and after in vitro culture without hormonal support to induce apoptosis indicated that mitochondrial cytochrome c release, a biochemical step required for the activation of apaf-1, occurs in granulosa cells cultured in vitro. |
1999-06-03 |
2023-08-12 |
mouse |
| A Rickers, N Peters, V Badock, R Beyaert, P Vandenabeele, B Dörken, K Bommer. Cleavage of transcription factor SP1 by caspases during anti-IgM-induced B-cell apoptosis. European journal of biochemistry. vol 261. issue 1. 1999-05-19. PMID:10103059. |
western blot analysis revealed two cleavage products of approximately 68 kda and 45 kda after induction of apoptosis. |
1999-05-19 |
2023-08-12 |
human |
| K M McGinnis, M E Gnegy, K K Wan. Endogenous bax translocation in SH-SY5Y human neuroblastoma cells and cerebellar granule neurons undergoing apoptosis. Journal of neurochemistry. vol 72. issue 5. 1999-05-06. PMID:10217266. |
we used western blot analysis to demonstrate that the proapoptotic protein bax translocated from the cytosolic to the mitochondrial fraction in sh-sy5y human neuroblastoma cells undergoing staurosporine- or egta-mediated apoptosis. |
1999-05-06 |
2023-08-12 |
human |
| C Gao, N Tsuchid. Activation of caspases in p53-induced transactivation-independent apoptosis. Japanese journal of cancer research : Gann. vol 90. issue 2. 1999-04-14. PMID:10189888. |
western blot analysis showed that parp, cpp32 and ich-1 precursors were cleaved during apoptosis. |
1999-04-14 |
2023-08-12 |
human |