| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| G C Gobé, J Halliday, I S Dunn, B V Harmon, E Schoch, S M Khan, D J Alla. Expression and localization of the retinoblastoma gene during radiation-induced apoptosis in neonatal rat kidney. Experimental cell research. vol 235. issue 2. 1997-10-16. PMID:9299159. |
we have used our knowledge of this model to analyze control (sham-treated) and irradiated renal tissue for rb mrna transcript levels and localization (northern blot and in situ hybridization (ish)), prb expression (western blot and immunolocalization), apoptosis and mitosis (light and electron microscopy, and dna gel electrophoresis for apoptosis), and cells in s-phase ([3h]thymidine uptake and autoradiography). |
1997-10-16 |
2023-08-12 |
rat |
| K Puranam, W H Qian, K Nikbakht, M Venable, L Obeid, Y Hannun, R M Boustan. Upregulation of Bcl-2 and elevation of ceramide in Batten disease. Neuropediatrics. vol 28. issue 1. 1997-08-20. PMID:9151319. |
this paper provides mechanistic support for the occurrence of apoptosis in this disease: there was marked upregulation of bcl-2 in brain from the late infantile and juvenile types at the protein and rna levels both by immunocytochemistry and by northern blot analysis; there were also a 42% to 197% increase in brain ceramide determinations in brains from three patients with the juvenile type and three patients with the late infantile type. |
1997-08-20 |
2023-08-12 |
Not clear |
| A Handa, T Kashimura, N Kawano, A Yamamoto, S Yoshida, I Jinnai, I Murohashi, M Bessho, K Hirashim. Granulocyte-colony stimulating factor induced apoptosis in radiation-induced murine leukemia cell line. Leukemia. vol 11 Suppl 3. 1997-08-07. PMID:9209400. |
to clarify the mechanism of the induction of apoptosis by g-csf we studied cell cycle and molecular changes in c2m-a5 cells cultured in medium with or without rhg-csf by means of using the flowcytometry and northern and western blot analyses. |
1997-08-07 |
2023-08-12 |
mouse |
| T Lynch, J P Vasilakos, K Raser, K M Keane, B D Shiver. Inhibition of the interleukin-1 beta converting enzyme family rescues neurons from apoptotic death. Molecular psychiatry. vol 2. issue 3. 1997-07-30. PMID:9152987. |
although ice was not detected by northern blot analysis, both cpp32 and nedd2 expression were found to increase during apoptosis. |
1997-07-30 |
2023-08-12 |
rat |
| D R Crawford, R J Lauzon, Y Wang, J E Mazurkiewicz, G P Schools, K J Davie. 16S mitochondrial ribosomal RNA degradation is associated with apoptosis. Free radical biology & medicine. vol 22. issue 7. 1997-07-29. PMID:9098105. |
rna extracted from control and apoptotic cultures, and analyzed by northern blot hybridization, revealed a significant increase in the degradation of mitochondrial 16s ribosomal rna (rrna) that was associated with apoptosis. |
1997-07-29 |
2023-08-12 |
Not clear |
| C M Whitacre, N A Berge. Factors affecting topotecan-induced programmed cell death: adhesion protects cells from apoptosis and impairs cleavage of poly(ADP-ribose)polymerase. Cancer research. vol 57. issue 11. 1997-07-10. PMID:9187115. |
as part of this study, we assessed the use of measuring poly(adp-ribose) polymerase (parp) cleavage by western blot, as an index of apoptosis, relative to measuring chromatin condensation by acridine orange analysis. |
1997-07-10 |
2023-08-12 |
human |
| S C Hsieh, M H Huang, C Y Tsai, Y Y Tsai, S T Tsai, K H Sun, H S Yu, S H Han, C L Y. The expression of genes modulating programmed cell death in normal human polymorphonuclear neutrophils. Biochemical and biophysical research communications. vol 233. issue 3. 1997-06-30. PMID:9168918. |
in order to understand the molecular basis of pmn apoptosis, the expression of apoptosis-related (fas, fas-ligand, p53, and c-myc) and survival-related (bcl-2) genes was detected by flow cytometry, western blot and reverse transcription-assisted polymerase chain reaction (rt-pcr). |
1997-06-30 |
2023-08-12 |
human |
| F Potvin, E Petitclerc, F Marceau, P E Poubell. Mechanisms of action of antimalarials in inflammation: induction of apoptosis in human endothelial cells. Journal of immunology (Baltimore, Md. : 1950). vol 158. issue 4. 1997-03-05. PMID:9029128. |
northern blot analysis revealed a rapidly increased expression of the bcl-x(s) gene without any change in the expression of the bcl-2 gene, indicating that huvecs under chloroquine were undergoing apoptosis. |
1997-03-05 |
2023-08-12 |
human |
| G Forloni, O Bugiani, F Tagliavini, M Salmon. Apoptosis-mediated neurotoxicity induced by beta-amyloid and PrP fragments. Molecular and chemical neuropathology. vol 28. issue 1-3. 1997-01-23. PMID:8871955. |
the intracellular cascade of events activated by peptides was investigated by northern blot and pcr analysis of expression of early genes (c-fos, c-jun, c-myc) and other proteins (p53, sgp-2 bcl-2, hsp70, ich-1) potentially involved in apoptosis. |
1997-01-23 |
2023-08-12 |
Not clear |
| X Ge, S Yannai, G Rennert, N Gruener, F A Fare. 3,3'-Diindolylmethane induces apoptosis in human cancer cells. Biochemical and biophysical research communications. vol 228. issue 1. 1996-12-13. PMID:8912651. |
western blot showed that wild-type p53 protein was unchanged after mcf-7 cells had been exposed to 50 microm 3,3'-diindolylmethane for 8 h. this study provides evidences that 3,3'-diindolylmethane induces apoptosis in human cancer cells and that the induction of apoptosis is independent of p53 pathway. |
1996-12-13 |
2023-08-12 |
human |
| H Yamada, M Nakagawa, I Higuchi, R Ohkubo, M Osam. Type II muscle fibers are stained by anti-Fas antibody. Journal of the neurological sciences. vol 134. issue 1-2. 1996-09-27. PMID:8747853. |
to examine whether apoptosis related proteins are present in skeletal muscles we studied biopsied muscles immunohistochemically and by western blot analysis. |
1996-09-27 |
2023-08-12 |
Not clear |
| Z Han, D Chatterjee, J Early, P Pantazis, E A Hendrickson, J H Wych. Isolation and characterization of an apoptosis-resistant variant of human leukemia HL-60 cells that has switched expression from Bcl-2 to Bcl-xL. Cancer research. vol 56. issue 7. 1996-05-13. PMID:8603411. |
western blot analysis demonstrated that the parental hl-60 cells expressed both bcl-2 and bax, two factors known to be intimately involved in the control of apoptosis. |
1996-05-13 |
2023-08-12 |
human |
| L Scaglia, F E Smith, S Bonner-Wei. Apoptosis contributes to the involution of beta cell mass in the post partum rat pancreas. Endocrinology. vol 136. issue 12. 1995-12-28. PMID:7588296. |
the expression of trpm 2 and tgf beta 1, often enhanced in models of apoptosis, were studied during the post partum period by northern blot analysis and immunohistochemistry. |
1995-12-28 |
2023-08-12 |
rat |
| G Häcker, D L Vau. Expression of candidate cell death genes in cell lines during apoptosis. Biochemistry and cell biology = Biochimie et biologie cellulaire. vol 72. issue 11-12. 1995-10-05. PMID:7654317. |
we have cloned mouse candidate cell death genes rp-2 and nedd2 and used northern blot analysis to study their expression in a growth-factor-dependent cell line (fdc-p1) that can be induced to undergo apoptosis by growth factor withdrawal and in a thymoma line (st4) that undergoes apoptosis when irradiated. |
1995-10-05 |
2023-08-12 |
mouse |
| J J Yen, Y C Hsieh, C L Yen, C C Chang, S Lin, H F Yang-Ye. Restoring the apoptosis suppression response to IL-5 confers on erythroleukemic cells a phenotype of IL-5-dependent growth. Journal of immunology (Baltimore, Md. : 1950). vol 154. issue 5. 1995-03-29. PMID:7868889. |
the following findings suggest that overexpression of the hil-5 receptor alpha-chain may be responsible for restoring the apoptosis suppression ability of il-5: 1) the growth of jytf-1 cells remained cytokine-dependent; 2) the proliferation of jytf-1 cells in il-5 was not mediated by autocrine secretion; 3) jytf-1 and tf-1 cells responded similarly to other cytokines such as human erythropoietin; 4) northern blot analysis revealed that jytf-1 cells expressed approximately eightfold more il-5 receptor alpha-chain mrna than did tf-1. |
1995-03-29 |
2023-08-12 |
human |
| A O Hueber, M Pierres, H T H. Quantitating apoptosis by a nonradioactive DNA dot blot assay. Analytical biochemistry. vol 221. issue 2. 1995-01-30. PMID:7810894. |
quantitating apoptosis by a nonradioactive dna dot blot assay. |
1995-01-30 |
2023-08-12 |
Not clear |
| W H Kim, H W Schnaper, M Nomizu, Y Yamada, H K Kleinma. Apoptosis in human fibrosarcoma cells is induced by a multimeric synthetic Tyr-Ile-Gly-Ser-Arg (YIGSR)-containing polypeptide from laminin. Cancer research. vol 54. issue 18. 1994-09-27. PMID:8069868. |
tumor cells treated with this peptide showed the expected morphological changes associated with apoptosis, acridine orange staining of nuclei, increased numbers of 3'-oh ends of dna in nuclei, a dna ladder pattern on agarose gels, and increased transforming growth factor beta 1 mrna by northern blot. |
1994-09-27 |
2023-08-12 |
human |
| W D Jarvis, A J Turner, L F Povirk, R S Traylor, S Gran. Induction of apoptotic DNA fragmentation and cell death in HL-60 human promyelocytic leukemia cells by pharmacological inhibitors of protein kinase C. Cancer research. vol 54. issue 7. 1994-04-28. PMID:7511048. |
finally, northern blot analysis revealed that exposure to calphostin c at a concentration that induced apoptosis (100 nm) failed to alter expression of bcl-2, an oncogene known to block apoptosis in both lymphoid and myeloid leukemia cells. |
1994-04-28 |
2023-08-12 |
human |
| A O Hueber, G Raposo, M Pierres, H T H. Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism. The Journal of experimental medicine. vol 179. issue 3. 1994-03-30. PMID:7906706. |
use of a quantitative dna dot blot assay indicated that thy-1-mediated thymocyte apoptosis was not blocked by rna or protein synthesis inhibitors, egta, or by cyclosporin a, and differed, therefore, from "activation-driven cell death". |
1994-03-30 |
2023-08-12 |
mouse |
| J Delic, M Morange, H Magdelena. Ubiquitin pathway involvement in human lymphocyte gamma-irradiation-induced apoptosis. Molecular and cellular biology. vol 13. issue 8. 1993-08-24. PMID:8393139. |
using in vitro nuclear transcription assays (run-on), northern (rna) blot analysis, immunolocalization studies, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis after immunoprecipitation, we demonstrate that (i) the ubiquitin mrna level is increased as a consequence of the activation of ubiquitin gene transcription 15 to 90 min after initiation of apoptosis; (ii) specifically in apoptotic cells, and not in all irradiated cells, nuclear proteins are highly ubiquitinated; and (iii) ubiquitin sequence-specific antisense oligonucleotide inhibition results in a decreased level of ubiquitinated nuclear proteins and considerably diminishes the proportion of cells exhibiting the apoptotic death pattern. |
1993-08-24 |
2023-08-12 |
human |